{"title":"基于全基因组测序的温州市食源性腹泻病例致泻性大肠杆菌患病率、耐药性及基因型分析","authors":"Panchi Shao, Xuejia Yang, Yuqin Hu, Huihuang Lou, Xuewei Liu, Haiyan Li, Leyi Zhang","doi":"10.1089/fpd.2025.0014","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> Although <i>Escherichia coli</i> can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. The study aims to investigate the prevalence, antimicrobial resistance, and genotyping of diarrheagenic <i>Escherichia coli</i> (DEC) in foodborne diarrhea cases in Wenzhou City in 2023, providing a scientific foundation for the monitoring and prevention of bacterial foodborne diseases. <b><i>Method:</i></b> Fecal samples from patients with diarrhea were collected from four sentinel hospitals for the isolation of DEC. The pathogenic types of DEC were identified using fluorescence quantitative polymerase chain reaction. Antimicrobial susceptibility testing was performed by broth microdilution, while whole-genome sequencing was utilized for genotyping. Clustering analysis based on the EnteroBase database was conducted to assess the genetic similarity of the strains. <b><i>Results:</i></b> Among 2591 fecal samples tested, 90 were positive for DEC, resulting in a detection rate of 3.47% (90/2591). The majority of DEC isolates were enterotoxigenic <i>E. coli</i> (ETEC) and enteroaggregative <i>E. coli</i> (EAEC), accounting for 87.78% (79/90) of the total. DEC detection showed a seasonal pattern, with the highest positivity observed in individuals aged 20-39 years. The genotyping of the most prevalent virulence genes in ETEC was <i>estIa/estIb</i> (57.50%, 23/40), while <i>aggR</i> (23.08%) was most common in EAEC. Genotyping revealed high genetic polymorphism among DEC strains, with HC1100_13 being the predominant clone. The prevalence of the resistance gene <i>blaEC</i> was 96.67%. The highest drug resistance rate was observed in ampicillin (68.89%), followed by nalidixic acid (51.11%), and the multidrug resistance rate was 65.56%. <b><i>Conclusion:</i></b> ETEC and EAEC were the most common DEC strains detected in Wenzhou in 2023. These strains exhibited significant genetic diversity and displayed considerable antimicrobial and multidrug resistance, highlighting the need for enhanced monitoring and intervention strategies.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence, Antimicrobial Resistance, and Genotypic Characterization of Diarrheagenic <i>Escherichia coli</i> in Foodborne Diarrhea Cases in Wenzhou Based on Whole-Genome Sequencing.\",\"authors\":\"Panchi Shao, Xuejia Yang, Yuqin Hu, Huihuang Lou, Xuewei Liu, Haiyan Li, Leyi Zhang\",\"doi\":\"10.1089/fpd.2025.0014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Background:</i></b> Although <i>Escherichia coli</i> can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. The study aims to investigate the prevalence, antimicrobial resistance, and genotyping of diarrheagenic <i>Escherichia coli</i> (DEC) in foodborne diarrhea cases in Wenzhou City in 2023, providing a scientific foundation for the monitoring and prevention of bacterial foodborne diseases. <b><i>Method:</i></b> Fecal samples from patients with diarrhea were collected from four sentinel hospitals for the isolation of DEC. The pathogenic types of DEC were identified using fluorescence quantitative polymerase chain reaction. Antimicrobial susceptibility testing was performed by broth microdilution, while whole-genome sequencing was utilized for genotyping. Clustering analysis based on the EnteroBase database was conducted to assess the genetic similarity of the strains. <b><i>Results:</i></b> Among 2591 fecal samples tested, 90 were positive for DEC, resulting in a detection rate of 3.47% (90/2591). The majority of DEC isolates were enterotoxigenic <i>E. coli</i> (ETEC) and enteroaggregative <i>E. coli</i> (EAEC), accounting for 87.78% (79/90) of the total. DEC detection showed a seasonal pattern, with the highest positivity observed in individuals aged 20-39 years. The genotyping of the most prevalent virulence genes in ETEC was <i>estIa/estIb</i> (57.50%, 23/40), while <i>aggR</i> (23.08%) was most common in EAEC. Genotyping revealed high genetic polymorphism among DEC strains, with HC1100_13 being the predominant clone. The prevalence of the resistance gene <i>blaEC</i> was 96.67%. The highest drug resistance rate was observed in ampicillin (68.89%), followed by nalidixic acid (51.11%), and the multidrug resistance rate was 65.56%. <b><i>Conclusion:</i></b> ETEC and EAEC were the most common DEC strains detected in Wenzhou in 2023. These strains exhibited significant genetic diversity and displayed considerable antimicrobial and multidrug resistance, highlighting the need for enhanced monitoring and intervention strategies.</p>\",\"PeriodicalId\":12333,\"journal\":{\"name\":\"Foodborne pathogens and disease\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Foodborne pathogens and disease\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1089/fpd.2025.0014\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2025.0014","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Prevalence, Antimicrobial Resistance, and Genotypic Characterization of Diarrheagenic Escherichia coli in Foodborne Diarrhea Cases in Wenzhou Based on Whole-Genome Sequencing.
Background: Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. The study aims to investigate the prevalence, antimicrobial resistance, and genotyping of diarrheagenic Escherichia coli (DEC) in foodborne diarrhea cases in Wenzhou City in 2023, providing a scientific foundation for the monitoring and prevention of bacterial foodborne diseases. Method: Fecal samples from patients with diarrhea were collected from four sentinel hospitals for the isolation of DEC. The pathogenic types of DEC were identified using fluorescence quantitative polymerase chain reaction. Antimicrobial susceptibility testing was performed by broth microdilution, while whole-genome sequencing was utilized for genotyping. Clustering analysis based on the EnteroBase database was conducted to assess the genetic similarity of the strains. Results: Among 2591 fecal samples tested, 90 were positive for DEC, resulting in a detection rate of 3.47% (90/2591). The majority of DEC isolates were enterotoxigenic E. coli (ETEC) and enteroaggregative E. coli (EAEC), accounting for 87.78% (79/90) of the total. DEC detection showed a seasonal pattern, with the highest positivity observed in individuals aged 20-39 years. The genotyping of the most prevalent virulence genes in ETEC was estIa/estIb (57.50%, 23/40), while aggR (23.08%) was most common in EAEC. Genotyping revealed high genetic polymorphism among DEC strains, with HC1100_13 being the predominant clone. The prevalence of the resistance gene blaEC was 96.67%. The highest drug resistance rate was observed in ampicillin (68.89%), followed by nalidixic acid (51.11%), and the multidrug resistance rate was 65.56%. Conclusion: ETEC and EAEC were the most common DEC strains detected in Wenzhou in 2023. These strains exhibited significant genetic diversity and displayed considerable antimicrobial and multidrug resistance, highlighting the need for enhanced monitoring and intervention strategies.
期刊介绍:
Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide.
Foodborne Pathogens and Disease coverage includes:
Agroterrorism
Safety of organically grown and genetically modified foods
Emerging pathogens
Emergence of drug resistance
Methods and technology for rapid and accurate detection
Strategies to destroy or control foodborne pathogens
Novel strategies for the prevention and control of plant and animal diseases that impact food safety
Biosecurity issues and the implications of new regulatory guidelines
Impact of changing lifestyles and consumer demands on food safety.