基于全基因组测序的温州市食源性腹泻病例致泻性大肠杆菌患病率、耐药性及基因型分析

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Panchi Shao, Xuejia Yang, Yuqin Hu, Huihuang Lou, Xuewei Liu, Haiyan Li, Leyi Zhang
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引用次数: 0

摘要

背景:虽然大肠杆菌可能是胃肠道中一种无害的居民,但它也具有致病能力,可引起严重的腹泻和肠外疾病。本研究旨在了解2023年温州市食源性腹泻病例中致泻性大肠杆菌(DEC)的流行情况、耐药性及基因分型,为细菌性食源性疾病的监测和预防提供科学依据。方法:收集4所哨点医院腹泻患者粪便标本,进行DEC的分离,采用荧光定量聚合酶链反应对DEC的病原类型进行鉴定。采用微量肉汤稀释法进行药敏试验,全基因组测序进行基因分型。基于EnteroBase数据库进行聚类分析,评估菌株的遗传相似性。结果:2591份粪便标本中,DEC阳性90份,检出率为3.47%(90/2591)。大肠杆菌以产肠毒素型大肠杆菌(ETEC)和肠聚集型大肠杆菌(EAEC)居多,占总数的87.78%(79/90)。DEC的检出率呈季节性变化,20 ~ 39岁人群检出率最高。ETEC中最常见的毒力基因为estIa/estIb (57.50%, 23/40), EAEC中最常见的毒力基因为aggR(23.08%)。基因分型结果显示,各菌株具有较高的遗传多态性,以HC1100_13为优势克隆。耐药基因blaEC阳性率为96.67%。耐药率最高的是氨苄西林(68.89%),其次是萘啶酸(51.11%),多药耐药率为65.56%。结论:2023年温州市检出的DEC以ETEC和EAEC最为常见。这些菌株表现出显著的遗传多样性,并表现出相当大的抗微生物和多药耐药性,突出了加强监测和干预策略的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence, Antimicrobial Resistance, and Genotypic Characterization of Diarrheagenic Escherichia coli in Foodborne Diarrhea Cases in Wenzhou Based on Whole-Genome Sequencing.

Background: Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. The study aims to investigate the prevalence, antimicrobial resistance, and genotyping of diarrheagenic Escherichia coli (DEC) in foodborne diarrhea cases in Wenzhou City in 2023, providing a scientific foundation for the monitoring and prevention of bacterial foodborne diseases. Method: Fecal samples from patients with diarrhea were collected from four sentinel hospitals for the isolation of DEC. The pathogenic types of DEC were identified using fluorescence quantitative polymerase chain reaction. Antimicrobial susceptibility testing was performed by broth microdilution, while whole-genome sequencing was utilized for genotyping. Clustering analysis based on the EnteroBase database was conducted to assess the genetic similarity of the strains. Results: Among 2591 fecal samples tested, 90 were positive for DEC, resulting in a detection rate of 3.47% (90/2591). The majority of DEC isolates were enterotoxigenic E. coli (ETEC) and enteroaggregative E. coli (EAEC), accounting for 87.78% (79/90) of the total. DEC detection showed a seasonal pattern, with the highest positivity observed in individuals aged 20-39 years. The genotyping of the most prevalent virulence genes in ETEC was estIa/estIb (57.50%, 23/40), while aggR (23.08%) was most common in EAEC. Genotyping revealed high genetic polymorphism among DEC strains, with HC1100_13 being the predominant clone. The prevalence of the resistance gene blaEC was 96.67%. The highest drug resistance rate was observed in ampicillin (68.89%), followed by nalidixic acid (51.11%), and the multidrug resistance rate was 65.56%. Conclusion: ETEC and EAEC were the most common DEC strains detected in Wenzhou in 2023. These strains exhibited significant genetic diversity and displayed considerable antimicrobial and multidrug resistance, highlighting the need for enhanced monitoring and intervention strategies.

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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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