Emergence and Characterization of the High-Level Tigecycline Resistance Gene tet(X4) in Salmonella enterica Serovar Rissen from Food in China.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Hongzhi Zhang, Wenjie Chen, Xiaoyu Lu, Yingying Liang, Xiao Quan, Xin Liu, Tianqi Shi, Ying Yu, Ruichao Li, Huanyu Wu
{"title":"Emergence and Characterization of the High-Level Tigecycline Resistance Gene <i>tet</i>(X4) in <i>Salmonella enterica</i> Serovar Rissen from Food in China.","authors":"Hongzhi Zhang, Wenjie Chen, Xiaoyu Lu, Yingying Liang, Xiao Quan, Xin Liu, Tianqi Shi, Ying Yu, Ruichao Li, Huanyu Wu","doi":"10.1089/fpd.2024.0101","DOIUrl":null,"url":null,"abstract":"<p><p>The plasmid-mediated <i>tet</i>(X4) gene has exhibited a high-level resistance to tigecycline (TGC), which has raised concerns globally regarding antibiotic resistance. Although the widespread <i>tet</i>(X4) has been found widely in <i>Escherichia coli</i>, it is scarcely found in other Enterobacteriaceae. This study aimed to characterize a ST469 <i>Salmonella enterica</i> serovar Rissen (<i>S.</i> Rissen) isolate harboring <i>tet</i>(X4) from pork, which was identified and characterized via antimicrobial susceptibility testing, conjugation assays, plasmid curing testing, whole-genome sequencing, and bioinformatic analysis. Ten ST469 <i>S.</i> Rissen isolates of 223 <i>Salmonella</i> spp. isolates were isolated from food samples in China during 2021-2023. One of 10 <i>S</i>. Rissen isolates, SM2301, carrying <i>tet</i>(X4) conferred high-level resistance to TGC (minimum inhibitory concentration > 8 µg/mL). The <i>tet</i>(X4) could be conjugated into different recipients, including <i>E. coli</i>, <i>S. enteritidis</i>, and <i>K. pneumoniae</i> isolates. Plasmid curing confirmed that <i>tet</i>(X4) was plasmid-mediated. Genetic analysis revealed that the <i>tet</i>(X4) in the SM2301 isolate was located in the IncFIA(HI1)-IncHI1A-IncHI1B(R27) hybrid plasmid, and the structure of <i>tet</i>(X4) was <i>abh</i>-<i>tet</i>(X4)-IS<i>CR2</i>. To the best of our knowledge, this is the first report of a <i>tet</i>(X4)-positive food-derived <i>S. Rissen</i> isolate. The extending bacterial species of <i>tet</i>(X4)-bearing plasmids suggested the increasing transmission risk of the mobile TGC resistance gene <i>tet</i>(X4) beyond <i>E. coli</i>. This study highlights the emerging and evolution risk of novel resistance genes across various bacterial species. Therefore, further surveillance is warranted to monitor the prevalence of <i>tet</i>(X4) in <i>Salmonella</i> spp. and other bacterial species.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2024.0101","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The plasmid-mediated tet(X4) gene has exhibited a high-level resistance to tigecycline (TGC), which has raised concerns globally regarding antibiotic resistance. Although the widespread tet(X4) has been found widely in Escherichia coli, it is scarcely found in other Enterobacteriaceae. This study aimed to characterize a ST469 Salmonella enterica serovar Rissen (S. Rissen) isolate harboring tet(X4) from pork, which was identified and characterized via antimicrobial susceptibility testing, conjugation assays, plasmid curing testing, whole-genome sequencing, and bioinformatic analysis. Ten ST469 S. Rissen isolates of 223 Salmonella spp. isolates were isolated from food samples in China during 2021-2023. One of 10 S. Rissen isolates, SM2301, carrying tet(X4) conferred high-level resistance to TGC (minimum inhibitory concentration > 8 µg/mL). The tet(X4) could be conjugated into different recipients, including E. coli, S. enteritidis, and K. pneumoniae isolates. Plasmid curing confirmed that tet(X4) was plasmid-mediated. Genetic analysis revealed that the tet(X4) in the SM2301 isolate was located in the IncFIA(HI1)-IncHI1A-IncHI1B(R27) hybrid plasmid, and the structure of tet(X4) was abh-tet(X4)-ISCR2. To the best of our knowledge, this is the first report of a tet(X4)-positive food-derived S. Rissen isolate. The extending bacterial species of tet(X4)-bearing plasmids suggested the increasing transmission risk of the mobile TGC resistance gene tet(X4) beyond E. coli. This study highlights the emerging and evolution risk of novel resistance genes across various bacterial species. Therefore, further surveillance is warranted to monitor the prevalence of tet(X4) in Salmonella spp. and other bacterial species.

中国食品中瑞森沙门氏菌对替加环素的高水平耐药基因 tet(X4) 的出现及其特征。
质粒介导的 tet(X4)基因对替加环素(TGC)具有高度耐药性,这引起了全球对抗生素耐药性的关注。虽然大肠杆菌中广泛存在 tet(X4) 基因,但在其他肠杆菌科细菌中却很少发现。本研究旨在通过抗菌药物敏感性试验、共轭试验、质粒固化试验、全基因组测序和生物信息学分析,鉴定猪肉中携带 tet(X4)的 ST469 瑞森沙门氏菌(S. Rissen)分离株的特征。2021-2023 年期间,从中国的食品样本中分离出了 223 株沙门氏菌属分离物中的 10 株 ST469 S. Rissen 分离物。在10株里森沙门氏菌分离株中,有一株(SM2301)携带tet(X4),对TGC具有高水平耐药性(最低抑菌浓度> 8 µg/mL)。tet(X4)可连接到不同的受体中,包括大肠杆菌、肠炎双球菌和肺炎双球菌分离物。质粒固化证实了 tet(X4) 是由质粒介导的。基因分析表明,SM2301 分离物中的 tet(X4) 位于 IncFIA(HI1)-IncHI1A-IncHI1B(R27) 杂交质粒中,tet(X4) 的结构为 abh-tet(X4)-ISCR2 。据我们所知,这是首次报道源于食物的利森氏菌的 tet(X4)阳性分离物。携带 tet(X4) 质粒的细菌种类的增加表明,移动 TGC 抗性基因 tet(X4) 的传播风险正在增加,已超出大肠杆菌的范围。这项研究凸显了新型抗性基因在不同细菌物种中的出现和进化风险。因此,有必要进一步监测 tet(X4) 在沙门氏菌属和其他细菌物种中的流行情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信