The Identification, Antibiotic Resistance, Virulence Characteristics, and Genome Analysis of Cronobacter spp. Isolated from Infant Rice Cereal in Nanchang, China.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2025-05-20 DOI:10.1089/fpd.2025.0002

Rui Li, Huifen Jiang, Xiaosi Wang, Pingping Zhai, Xin Wu, Jiajun Wei

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引用次数: 0

Abstract

Cronobacter spp. are well known to be resistant to osmotic and dry stresses and can persist in a variety of foods. C. sakazakii and C. malonaticus are currently considered to be the primary pathogenic species that cause severe diseases. There are few studies on the prevalence of Cronobacter spp. in infant rice cereal. Information on the diversity, pathogenicity, and virulence of Cronobacter species obtained from foods is still relatively scarce and fragmentary. In this article, a total of 67 Cronobacter spp. strains were isolated from infant rice cereal in Nanchang, China. Forty-seven strains of C. sakazakii and 11 strains of C. malonaticus were identified by polymerase chain reaction (PCR). The resistance to 10 antibiotics was analyzed, showing that all isolates were sensitive except two strains. PCR detection for the five virulence genes (cpa, aut, hly, inv, and sip) and whole genome sequencing were performed. Co-existence of CSA or CMA with ampH was found in C. sakazakii and C. malonaticus strains. Compared with C. sakazakii, C. malonaticus lacked various virulence genes including yeeJ, stjC, and nanAKT genes. Another important observation was the presence of csg in the C. malonaticus genomes, while it was not found in the C. sakazakii. Notably, STs 1, 7, and 4, which are frequently associated with clinical infections, were observed. C. sakazakii and C. malonaticus strains induced higher apoptosis rate in Caco-2 cell in comparison with other Cronobacter species.

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南昌婴儿米粒克罗诺杆菌的鉴定、耐药性、毒力特征及基因组分析

克罗诺杆菌是众所周知的抗渗透和干燥胁迫，并能持续在各种食物。sakazaki C.和C. malonaticus目前被认为是引起严重疾病的主要致病物种。关于克罗诺杆菌在婴儿米粉中的流行情况的研究很少。从食物中获得的关于克罗诺杆菌种类的多样性、致病性和毒力的信息仍然相对缺乏和不完整。本文从南昌的婴儿米粉中分离到67株克罗诺杆菌。采用聚合酶链式反应（PCR）鉴定出47株阪崎弧菌和11株丙酸弧菌。对10种抗生素的耐药性分析表明，除2株外，其余菌株均敏感。PCR检测5个毒力基因（cpa、aut、hly、inv、sip），并进行全基因组测序。在阪崎c菌和丙酸c菌中发现CSA或CMA与ampH共存。与阪崎弧菌相比，malonaticus缺乏多种毒力基因，包括yeeJ、stjC和nanAKT基因。另一个重要的观察结果是在C. malonaticus基因组中存在csg，而在C. sakazaki基因组中没有发现csg。值得注意的是，STs 1、7和4通常与临床感染有关。与其他克罗诺杆菌相比，阪崎C.和丙酸C.诱导Caco-2细胞的凋亡率更高。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.