Environmental toxicology and pharmacology最新文献

{"title":"Early life developmental effects induced by dioxins and PCBs in novel bioassays with C. elegans","authors":"Cong Bao ,&nbsp;Antoine Karengera ,&nbsp;Jan Kammenga ,&nbsp;Inez Dinkla ,&nbsp;Willemien Wieland ,&nbsp;AlberTinka J. Murk","doi":"10.1016/j.etap.2025.104697","DOIUrl":"10.1016/j.etap.2025.104697","url":null,"abstract":"<div><div>This study assessed the effects of TCDD, two PCB mixtures (Clophen A50 and Aroclor 1254), and field extracts from marine sediments and swimming crab tissues on early-life development in Caenorhabditis elegans. Gravid nematodes were exposed on agar, and isolated eggs and larvae were tested in solution. Larval development was evaluated after 72 hours. Reporter gene assays (DR-CALUX) were also used to quantify dioxin-equivalent toxicity (TEQ). Exposure to 10 pM Clophen A50 and TCDD on agar inhibited L3–L4 transition by 60 % and 50 %, respectively. Liquid exposure to 5 µM Aroclor 1254 or TCDD (10 nM and 10 µM) delayed development by 20–40 %. Field extracts contained TEQ values of 0.67–4.91 ng/kg (0.2–1.47 pM TCDD), reducing L3–L4 development by 40–60 %. Both bioassays effectively assessed the toxicity of persistent organic pollutants in environmental samples. Agar exposure mimics realistic uptake, while liquid assays offer faster, high-throughput screening.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104697"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide residues in honey: Agricultural landscapes and commercial wax foundation sheets as potential routes of chronic exposure for honey bees","authors":"Deicy C. Munoz Agudelo ,&nbsp;Hannah Whitehead ,&nbsp;Laura L. Figueroa ,&nbsp;Lynn S. Adler","doi":"10.1016/j.etap.2025.104700","DOIUrl":"10.1016/j.etap.2025.104700","url":null,"abstract":"<div><div>Pesticides pose significant threats to pollinators, and honey bees are frequently exposed through foraging and beekeeping practices. We assessed honey bee pesticide exposure by analyzing 92 pesticide residues in honey from 30 hobbyist apiaries across Massachusetts, along with store-bought honey and commercial wax foundation. For all samples, we calculated the risk of multiresidue toxicity to honey bees and assessed the role of landscape composition in predicting pesticides in local honey. Both honey and wax contained multiple pesticides, particularly neonicotinoids and piperonyl butoxide. Store-bought honey accumulated at least two times more residues than local, but did not differ significantly in toxicity. Overall, honey toxicity levels remained below thresholds of concern for bees and human consumption. Although our study had low agricultural land (∼6 %), croplands were positively correlated with pesticides in honey, while wetlands (∼ 15 %) were negatively correlated. Additionally, our study suggests that commercial wax exacerbates pesticide exposure.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104700"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruptive ecotoxicological effects of fluoxetine on serotoninergic signaling and enteric neurogenesis in early zebrafish larvae (Danio rerio)","authors":"Kainã Rocha Cabrera Fagundes ,&nbsp;Natalia Kasica ,&nbsp;Małgorzata Potoczna ,&nbsp;Shiho Okitsu-Sakurayama ,&nbsp;Piotr Podlasz ,&nbsp;Renata de Britto Mari","doi":"10.1016/j.etap.2025.104698","DOIUrl":"10.1016/j.etap.2025.104698","url":null,"abstract":"<div><div>This study investigated the multilevel effects of environmentally relevant concentrations of fluoxetine on serotonergic signaling and enteric neurogenesis in early zebrafish larvae (Danio rerio). To this end, zebrafish were exposed to various concentrations of fluoxetine for four days, from the 1,000-cell stage to 4 days post-fertilization (dpf).Following exposure, whole larvae were subjected to molecular, morphological, and behavioral analyses. All tested concentrations led to upregulation of the serotonin transporter (slc6a4a). At intermediate concentrations, overexpression of the serotonin receptor htr1aa was observed. The highest concentration caused a reduced total enteric neurons density, while the intermediate concentration reduced the density of serotonergic enteric neurons. Additionally, the highest concentration decreased larval locomotion and impaired their ability to differentiate between light and dark phases.Across all tested concentrations, fluoxetine disrupted serotonergic signaling, impaired enteric neurogenesis, and induced sedative-like behavioral effects.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104698"},"PeriodicalIF":4.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cu(ATSM) on the expression and activity of ABC export proteins in killifish (Fundulus heteroclitus) kidney tubules","authors":"Lisa Kraus ,&nbsp;Gert Fricker","doi":"10.1016/j.etap.2025.104696","DOIUrl":"10.1016/j.etap.2025.104696","url":null,"abstract":"<div><div>ABC transporters are important for excretion of xenobiotics and metabolites into urine. They are sensitive to metallic pollutants like cadmium, mercury, zinc, or arsenic. Here, we show that copper (Cu(ATSM)) stimulates ABC transporter-mediated export in isolated renal proximal tubules from Atlantic killifish (<em>Fundulus heteroclitus</em>) with main focus on Mrp2. Transporter stimulation was reduced by cycloheximide (CHX), an inhibitor of translation, suggesting that it is partially caused by induced expression. Functional activation was reversed by modulators of the endothelin receptor (ET_B)/nitric oxide synthase/protein kinase C signaling pathway. Transporter activating effects were reversed by Gö6976 and peptide C2–4, both being PKCα inhibitors. Cu(ATSM)-induced activation was further suppressed by phosphatidylinositol 3-kinase inhibitor LY-294002 and mTOR inhibitor rapamycin. Activation was also inhibited by GSK650394, an inhibitor of serum-and-glucocorticoid-inducible-kinase-1 being a subsequent target. Given the parallelism with other metals, this ABC transporter regulation appears to be a general defense mechanism of teleosts to react on metallic pollutants.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104696"},"PeriodicalIF":4.2,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isatin-linked pyrazole K1 derivative alter the phosphatidylinositol-3-kinase pathway by enhancing the metabolic function and folliculogenesis in the triclosan-induced PCOS-like condition in zebrafish model","authors":"Karthikeyan Ramamurthy ,&nbsp;Marapatla Shiny ,&nbsp;S. Madesh ,&nbsp;Santhanam Sanjai Dharshan ,&nbsp;Girija Sastry Vedula ,&nbsp;Mansour K. Gatasheh ,&nbsp;Kathiravan Muthu Kumaradoss ,&nbsp;Jesu Arockiaraj","doi":"10.1016/j.etap.2025.104695","DOIUrl":"10.1016/j.etap.2025.104695","url":null,"abstract":"<div><div>Polycystic ovarian syndrome (PCOS), which causes hormonal imbalance, inflammation, and metabolic disorders, requires several treatments. This study aimed to examine the Isatin-linked pyrazole K1 derivative's effectiveness in PCOS induced by environmental contaminants such as triclosan, specifically assessing its biochemical, metabolic, and reproductive impacts. Isatin-linked pyrazole K1 derivative was synthesised in the lab and tested <em>in vitro</em> and <em>in vivo</em>, including cytotoxicity testing in CHO cells, apoptosis analysis in AO/PI staining, and developmental toxicity in zebrafish embryos. In addition, for network pharmacology analysis, BindingDB, GeneCard, and other databases were used to characterise the interaction of K1 derivative with PCOS-related genes and pathways, followed by examining the apoptosis in CHO cells, estimation of total cholesterol and triglycerides in adipose tissue of zebrafish. Furthermore, GSI%, follicular stage examination, collagen accumulation, nucleic acid staining by toluidine blue, and gene expression of <em>cyp19a1a</em>, <em>dennd1a</em>, <em>tox3</em>, <em>pik3ca</em>, and <em>pik3cd</em> were examined. The research found that K1 reduces various PCOS pathologies, improving folliculogenesis, overall ovarian function, and follicular growth. K1 treatment at 25 µM significantly enhanced SOD (1.470 ± 0.01533 U/ml), CAT (1.174 ± 0.008687 U/ml), and GSH (1.375 ± 0.006409 U/ml) levels while reducing LDH activity (0.9815 ± 0.01273 nmol/mg), demonstrating its ability to mitigate oxidative stress and cellular damage. In particular, K1 modulates insulin sensitivity by reducing the blood glucose level in PCOS-induced fish and lowering lipid levels, which is essential for treating PCOS metabolic symptoms. K1 derivative also significantly reduced collagen deposition in ovarian tissues, indicating K1 may reduce PCOS-related fibrosis, which suggests that the derivative may be a novel therapeutic agent for PCOS. The comprehensive approach of K1 addresses metabolic and reproductive concerns; however, clinical studies must be conducted to test these findings' efficacy and safety and understand its therapeutic molecular processes.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104695"},"PeriodicalIF":4.2,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lithium on mortality and metabolite profiles in Drosophila lithium-inducible SLC6 transporter mutants","authors":"Junko Kasuya ,&nbsp;Karina Kruth ,&nbsp;Dongkeun Lee ,&nbsp;Jong Sung Kim ,&nbsp;Aislinn Williams ,&nbsp;Toshihiro Kitamoto","doi":"10.1016/j.etap.2025.104684","DOIUrl":"10.1016/j.etap.2025.104684","url":null,"abstract":"<div><div>Lithium has long been the primary treatment for bipolar disorder and shows promise for managing other neurological and psychiatric conditions. We previously identified the <em>Lithium-inducible SLC6 transporter</em> (<em>List</em>) in <em>Drosophila melanogaster</em> as a gene significantly upregulated in response to lithium chloride supplementation. <em>List</em> encodes a putative amino acid transporter belonging to the Na⁺-dependent solute carrier family 6. Here, we show that <em>List</em> is expressed in the Malpighian tubules, glia, and hindgut. RNA interference-mediated <em>List</em> knockdown in the Malpighian tubules drastically increases lithium-induced mortality. Additionally, <em>List</em> loss-of-function mutants (<em>List</em>^{<em>TG4.2</em>}) accumulate six times more internal lithium than controls after lithium exposure. Metabolomic analysis revealed disrupted amino acid metabolism and a shift toward a more oxidized cellular redox state in lithium-treated <em>List</em>^{<em>TG4.2</em>} mutants. Overall, our findings suggest that <em>List</em> protects flies from lithium toxicity by regulating internal lithium levels and maintaining metabolic and redox balance.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104684"},"PeriodicalIF":4.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental pollutants and genetic toxicity: From mechanisms to human studies","authors":"Hilmi Orhan,&nbsp;Marcus S. Cooke","doi":"10.1016/j.etap.2025.104681","DOIUrl":"10.1016/j.etap.2025.104681","url":null,"abstract":"","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"115 ","pages":"Article 104681"},"PeriodicalIF":4.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of specific responses in the combined exposure of freshwater mussel Unio tumidus to psychoactive substances and microplastics","authors":"K. Yunko ,&nbsp;V. Martyniuk ,&nbsp;L. Gnatyshyna ,&nbsp;V. Khoma ,&nbsp;T. Matskiv ,&nbsp;H. Tulaidan ,&nbsp;O. Mykhalyuk ,&nbsp;R. Karitonas ,&nbsp;B. Gylyte ,&nbsp;L. Manusadžianas ,&nbsp;O. Stoliar","doi":"10.1016/j.etap.2025.104682","DOIUrl":"10.1016/j.etap.2025.104682","url":null,"abstract":"<div><div>The environmentally relevant aquatic pollution is associated with the mixtures of xenobiotics, each in the low, picomolar to micromolar concentrations. Among these substances, the combinations of pharmaceuticals and microplastics (MP) have become an increasingly serious threat. The objective of this study was to track the specific and multi-stress responses of swollen river mussels <em>(Unio tumidus</em>) to the psychoactive substances caffeine (Caff) and chlorpromazine (Cpz) under combined exposure with MP. The MP (1 mg·L⁻¹, size 35–50 μm), Caff (20 µg·L⁻¹), Cpz (12 ng·L⁻¹) or their mixture (Mix) were administered to mussels for 14 days. The redox state, enzymes of biotransformation and apoptosis were analysed in the digestive gland. All exposures except Mix caused oxidative injury to lipids and proteins, accompanied by increased GSH and metallothionein levels, suppressed NAD⁺ and activation of GST (except Mix), and GTPase. MP had the lower particular impact. Specific responses to Caff were activation of <em>Cyp</em>450 (EROD) and cathepsin D, decreased GSH/GSSG ratio and prominent demetallation of metallothionein. The Cpz caused an increase in NADH/NAD⁺ ratio and caspase-3 inhibition. In the combined exposure, the specific responses to single xenobiotics were alleviated which was confirmed by discriminant analysis. The Mix-group was distinguished by the highest NADH/NAD⁺ and GSH/GSSG ratios, markedly increased caspase-3 activity accompanied by the decrease of protein carbonyl level and the highest IBR index, attesting to the negative cumulative effect of multi-stress exposure. The vulnerability of mussels to pM concentration of neuroleptic Cpz needs particular attention.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104682"},"PeriodicalIF":4.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental pollution and health risks to informal workers: Exposure to heavy metals in human hair at Indonesian landfills","authors":"Azham Umar Abidin ,&nbsp;Fina Binazir Maziya ,&nbsp;Septian Hadi Susetyo ,&nbsp;Arvi Tri Sulistiyani ,&nbsp;Minoru Yoneda ,&nbsp;Taiki Nagaya ,&nbsp;Yasuto Matsui","doi":"10.1016/j.etap.2025.104685","DOIUrl":"10.1016/j.etap.2025.104685","url":null,"abstract":"<div><div>The concentration of heavy metals (HMs) in human hair is a bioindicator of environmental pollution over a long period from worker, including the informal worker at landfills. A landfill is where final-stage refuse is deposited, transported, and disposed of solid waste from urban areas. No study has been conducted on heavy metals in landfills and their health impact on informal workers. This study aims to investigate the accumulation of heavy metals over a long period, the effect of toxic metal exposure on the human body, and the health risks informal workers perceive as bioindicators of pollution in landfills. Human hair samples collected from informal workers and urban were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) to measure HMs and human hair certified reference material (CRM) from the national institute for environmental studies, Japan. The sample consisted of individuals who were interviewed and had their hair cut. The total number of respondents in this study was 51 people. The average concentrations of the element heavy metals (µg/g) in informal workers are shown: Pb 4.70, As 0.30, Cd 1.07, Mn 14.58, Co 0.14, Cu 19.31, Fe 73.05, Zn 646.2, and Se 0.65. The results of our study the concentrations of heavy metals were higher than those found for urban residents. In the education level variable, 17.4 % of respondents did not attend school, 50 % attended primary school, and 32.6 % attended junior high school. Gender of respondents, 71.7 % are female, and in the working period, 69.6 % are more than ten years old. An epidemiological study and statistical analysis show the respondents' symptoms &amp; health complaints such as cough, breathlessness, headache, nose irritation, variable mask, duration using a mask, and characteristics of the respondent's significant correlations p-value &lt; 0.05 with a concentration of all metals. These findings show that the HMs have contaminated the environment and entered the human bodies, posing health risks to informal workers in the landfill working area.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104685"},"PeriodicalIF":4.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PM10 exposure in beauty salons: impact on A549 cell viability, cell cycle, oxidative stress, and mutagenicity","authors":"Daniela Figueiredo ,&nbsp;Estela D. Vicente ,&nbsp;Cátia Gonçalves ,&nbsp;Isabel Lopes ,&nbsp;Célia A. Alves ,&nbsp;Helena Oliveira","doi":"10.1016/j.etap.2025.104683","DOIUrl":"10.1016/j.etap.2025.104683","url":null,"abstract":"<div><div>Beauty salons are unique indoor environments where elevated PM₁₀ levels may arise from frequent use of personal care and cosmetic products, potentially exposing salon workers and clients to harmful chemicals. This study aimed to assess the biological effects of PM₁₀ collected indoors and outdoors of a beauty salon, as well as the mutagenic potential of PM₁₀-bound polycyclic aromatic hydrocarbons (PAHs). Using the MTT test, a dose-dependent decrease in cell viability was observed in human alveolar adenocarcinoma cells (A549) exposed to PM₁₀, with indoor samples causing more pronounced effects than outdoor and background air. Indoor PM₁₀ exposure also increased intracellular reactive oxygen species (ROS) and caused an arrest in the G1 phase of cell cycle. These biological responses were correlated with the concentrations of various compounds, including organic carbon (OC), aliphatic carboxylic acids, dicarboxylic acids, and alkyl esters of fatty acids. No mutagenic effects were observed for all PAH samples tested using the <em>Salmonella typhimurium</em> strain TA98. The findings suggest that beauty salons might have elevated levels of PM₁₀, capable of inducing cytotoxic and oxidative stress-related effects and cell cycle disruption. Although no mutagenic activity was detected, the presence of harmful chemicals highlights potential health risks for workers and clients.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104683"},"PeriodicalIF":4.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}