Environmental toxicology and pharmacology最新文献

{"title":"Assessment of genetic damage levels in agricultural workers exposed to pesticides in Paraíba, Brazil","authors":"Laís Campos Teixeira de Carvalho-Gonçalves ,&nbsp;Patricianny Barreto de Góis Santos ,&nbsp;Glauciane Andrade da Silva ,&nbsp;Lucas Monteiro Bezerra Pinheiro ,&nbsp;Thiago Marcel Galdino Olinto ,&nbsp;Adegildo Rolim de Abreu Junior ,&nbsp;Márcia Helena Pontieri ,&nbsp;Solange Maria de Vasconcelos ,&nbsp;Jéssica Pedrosa de Lima ,&nbsp;Luiz Gualberto de Andrade Sobrinho ,&nbsp;Angela Lima Meneses de Queiroz ,&nbsp;Márcia Aparecida Cezar ,&nbsp;Juan Carlos Ramos Gonçalves","doi":"10.1016/j.etap.2025.104715","DOIUrl":"10.1016/j.etap.2025.104715","url":null,"abstract":"<div><div>Pesticide use has transformed agriculture, but improper handling poses serious health and environmental risks. Brazil, a top global pesticide consumer, faces alarming unapproved usage—nearly 80 % of pesticides sold lack approval in at least three OECD countries. This study investigates genotoxic effects on farmers in Paraíba, Brazil, analyzing buccal mucosa cells for DNA and cellular damage. Using the Buccal Micronucleus Cytome (BMCyt) assay and comet assay, we compared 33 pesticide-exposed farmers to 29 controls. The findings revealed significantly higher frequencies of cellular alterations and DNA damage among exposed farmers relative to the control group, with no significant impact from factors such as smoking, alcohol consumption, or family cancer history. These results underscore the genotoxic risks linked to prolonged pesticide exposure and highlight the necessity for stricter regulatory measures as well as the need for further large-scale studies to mitigate health risks within agricultural communities.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104715"},"PeriodicalIF":4.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of PDYN 68-bpVNTR, BDNF rs6265, OPRD1 rs569356 and OPRM1 rs2075572 polymorphisms on opioid-methamphetamine co-use","authors":"Merve Ak ,&nbsp;Gizem Özer-Uzaldı ,&nbsp;Mukaddes Asena Yıldırım ,&nbsp;Selin Özkan-Kotiloğlu ,&nbsp;Mustafa Danışman ,&nbsp;Dilek Kaya-Akyüzlü","doi":"10.1016/j.etap.2025.104714","DOIUrl":"10.1016/j.etap.2025.104714","url":null,"abstract":"<div><div>To investigate the impact of <em>PDYN</em> 68-bpVNTR, <em>BDNF</em> rs6265, <em>OPRD1</em> rs569356 and <em>OPRM1</em> rs2075572 variations on the susceptibility to opioid-methamphetamine co-use, a total of 532 individuals, including opioid (n = 104), and methamphetamine (n = 166) users as well as opioid and methamphetamine co- users (n = 158) and healthy individuals (n = 104), were included. <em>BDNF</em> rs6265, <em>OPRD1</em> rs569356 and <em>OPRM1</em> rs2075572 were genotyped by PCR-RFLP method, while <em>PDYN</em> 68-bp VNTR was genotyped by PCR. The assessment of impulsiveness, craving, withdrawal, anxiety and depressive symptoms was conducted using scales. A significant difference was identified between the substance-using groups and the control group in view of the alleles of <em>PDYN</em> 68-bp VNTR (p = 0.001). The allele frequencies of the <em>PDYN</em> 68-bp VNTR exhibited a difference between opioid and methamphetamine users (p = 0.018). The 5-repeat allele was first observed in the Turkish population. The analysed polymorphisms could contribute to the development of addiction, exerting an influence on impulsiveness, craving and withdrawal.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104714"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined exposure to atrazine and phoxim exacerbated the alterations of enzyme activity and abnormal gene expression in earthworms (Eisenia fetida)","authors":"Jinzhan Li ,&nbsp;Jingwen Wang ,&nbsp;Fang Shu ,&nbsp;Nan Huang ,&nbsp;Ru Jia ,&nbsp;Yanhua Wang","doi":"10.1016/j.etap.2025.104713","DOIUrl":"10.1016/j.etap.2025.104713","url":null,"abstract":"<div><div>Multiple pesticides often coexist in soil, potentially leading to interactions among their components, these may detrimentally impact soil organisms. This study assessed the potential risks posed by simultaneous exposure to atrazine (ATR) and phoxim (PHO) on enzyme and transcription levels in earthworms (<em>Eisenia fetida</em>). The results revealed that ATR exhibited higher acute toxicity towards <em>E. fetida</em> compared to PHO, and their combined exposure resulted in a synergistic acute effect. Furthermore, low concentration combined exposure significantly stimulated catalase (CAT), malondialdehyde (MDA), and total superoxide dismutase (T-SOD) activities, which lead to more severe oxidative damage. Elevated expression levels of translationally controlled tumor protein (<em>tctp</em>) and calreticulin (<em>crt</em>) genes were observed in most exposed groups compared to the control. The synergistic effects of ATR and PHO on earthworms observed in this study may pose ecological risks to the soil ecosystem; thus, more attention should be paid to the joint effects of different pesticides.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104713"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term joint exposure of outdoor air pollutants and impaired kidney function in Korean adults: A mixture analysis based on a nationwide sample (2007–2019)","authors":"Seong-Uk Baek ,&nbsp;Jin-Ha Yoon","doi":"10.1016/j.etap.2025.104712","DOIUrl":"10.1016/j.etap.2025.104712","url":null,"abstract":"<div><div>We explored the association between exposure to air pollutant mixture and renal function. A nationwide sample of 69,066 Korean adults was analyzed. The 1-year moving averages of the concentrations of PM₁₀, PM_2.5, SO₂, NO₂, CO, and O₃ were estimated using the air pollution modeling. The outcome was estimated glomerular filtration rate (eGFR) value. Quantile-based G-computation analyses were performed to determine how a simultaneous increase in the air pollutant mixture by one quartile was associated with changes in eGFR levels. The mean of eGFR was 88.9 among the sample. In the quantile-based G-computation model, one-quartile increase in the levels of air pollutant mixture was linked to a decrease in eGFR by −0.85 (95 % CI: −1.14, −0.57), with CO and PM_2.5 contributing 52.3 % and 37.3 %, respectively, to the negative association between the pollutant mixture and eGFR. Our results suggest that long-term exposure to air pollutant mixtures is linked to impaired renal function.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104712"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tebuconazole-based fungicide impairs embryonic development of the South African Clawed Frog Xenopus laevis","authors":"Vanessa Ihle ,&nbsp;Hannah Flach ,&nbsp;Florian Kaminski,&nbsp;Petra Dietmann,&nbsp;Sarah Pfeffer,&nbsp;Susanne J. Kühl","doi":"10.1016/j.etap.2025.104708","DOIUrl":"10.1016/j.etap.2025.104708","url":null,"abstract":"<div><div>Tebuconazole (TBZ) is a broad-spectrum fungicide that disrupts fungal cell membrane. Due to its extensive application in agriculture, TBZ is frequently detected in aquatic ecosystems, posing potential risks to amphibians. However, the effects of TBZ-based formulations on amphibian development remain poorly understood. The present study investigates the effects of the TBZ-based fungicide Tebucur on the embryogenesis of the South African clawed frog. Two-cell stage embryos were exposed to varying concentrations of Tebucur (0.01–100 mg a.i./L) in a static non-renewal bioassay. The exposure time varied between four and 14 days, depending on the desired developmental stage of the embryos and the analysis method. Tebucur exposure resulted in mortality with a LC50-value of 8.0 mg a.i./L at 14 days. As well, various morphological abnormalities during neural and cardiac development emerged. Additionally, a reduction of overall mobility was observed. This underlines the need for strict regulation of the use of TBZ-formulations.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104708"},"PeriodicalIF":4.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide stress induces spermatozoa DNA damage and morphological abnormalities in Apis mellifera populations","authors":"Vesela Mitkovska ,&nbsp;Ivan Stoyanov ,&nbsp;Tsenka Chassovnikarova ,&nbsp;Penka Vasileva ,&nbsp;Plamen Petrov ,&nbsp;Evgeniya N. Ivanova","doi":"10.1016/j.etap.2025.104710","DOIUrl":"10.1016/j.etap.2025.104710","url":null,"abstract":"<div><div>The rising losses of honey bee colonies are causing public concern regarding the species' long-term sustainability. Therefore, this study aimed to assess how pesticide exposure affects DNA damage and morphological traits of drone spermatozoa across different <em>Apis mellifera</em> populations, categorized by varying colony loss rates. Chromatographic analysis identified 24 pesticide residues in bee samples and colony food stocks. Significant differences were found in comet assay parameters (tail intensity and Olive tail moment), frequency of morphological abnormalities, and morphometric variations in spermatozoa between apiaries experiencing high versus low mortality rates. The findings indicate that DNA damage and morphological abnormalities in spermatozoa, potentially caused by pesticide stress, compromise the reproductive ability of honey bee drones, contributing to the complex phenomenon of colony loss. The comet assay parameters and morphological aberrations of honey bee spermatozoa may serve as effective biomarkers for evaluating the fitness of drone spermatozoa and assessing environmental hazards to bee populations.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104710"},"PeriodicalIF":4.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic and metabolic effects of organic extracts from airborne fine particulate matter (PM2.5) in neuronal cells","authors":"Tatiana Silva ,&nbsp;Célia Alves ,&nbsp;Margarita G. Evtyugina ,&nbsp;Ana S. Dias ,&nbsp;Guilherme Martins Pereira ,&nbsp;Pérola de Castro Vasconcellos ,&nbsp;Maria de Fátima Andrade ,&nbsp;Helena Oliveira ,&nbsp;Iola F. Duarte","doi":"10.1016/j.etap.2025.104707","DOIUrl":"10.1016/j.etap.2025.104707","url":null,"abstract":"<div><div>Airborne fine particulate matter (PM_2.5) has been linked to neurological diseases, but its cellular and metabolic effects remain incompletely understood. This study assessed the cytotoxic and metabolic impact of PM_2.5 samples from São Paulo, Brazil, on SH-SY5Y neuroblastoma cells. Even at low toxicity levels (IC₁₀–IC₃₀), PM_2.5 organic extracts induced apoptosis, increased TNF-α secretion, and triggered moderate oxidative responses. Metabolomic analyses revealed a downregulation of energy-producing pathways, including glycolysis and the TCA cycle, along with decreased ATP and phosphocreatine levels. Compensatory adaptations were evident, such as increased proline oxidation, lipid accumulation, and activation of the creatine-phosphocreatine system. One-carbon metabolism was also affected, with changes suggesting suppression of the folate and methionine cycles. Elevated glutathione levels indicated an enhanced antioxidant response. These findings highlight how PM_2.5 disrupts neuronal energy homeostasis and redox balance, offering new insights into the cellular mechanisms of air pollution-related neurotoxicity.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104707"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are glyphosate or glyphosate-based herbicides linked to metabolic dysfunction-associated steatotic liver disease (MASLD)? The weight of current evidence","authors":"Luana Riechelmann-Casarin ,&nbsp;Leticia Cardoso Valente ,&nbsp;Rosemari Otton ,&nbsp;Luís Fernando Barbisan ,&nbsp;Guilherme Ribeiro Romualdo","doi":"10.1016/j.etap.2025.104705","DOIUrl":"10.1016/j.etap.2025.104705","url":null,"abstract":"<div><div>Metabolic dysfunction-associated steatotic liver disease (MASLD) affects around 30 % of the world’s population, increasing its prevalence by 50 % in the last three decades. MASLD pathogenesis is considered multiaxial, involving disturbances in the liver, adipose tissue (AT), and gut microbiome. In parallel with MASLD increasing trends, the total herbicide use has nearly tripled over the last three decades. Glyphosate (GLY) is the most used herbicide worldwide (825 mi kg/year). The intensive use of GLY-based herbicides (GBH) – largely driven by the adoption of glyphosate-tolerant genetically modified crops over the past two decades - has led to environmental (soil and water) and food contamination, resulting in continuous human exposure. Emerging (pre)clinical data highlights the significant implications of this herbicide on MASLD, marking a critical research area. Thus, this narrative review paper aimed at gathering and evaluating all epidemiological and (pre)clinical data on the implications of GLY or GBH on MASLD outcomes. Our work encompassed literature published between 2008–2025. Human urinary GLY levels are associated with different MASLD outcomes (steatosis risk, advanced fibrosis, increased transaminases) and comorbidities (higher risk for metabolic syndrome, diabetes, obesity and cardiovascular diseases) (6 studies). <em>In vitro</em> data indicate that GBH/GLY cause oxidative stress, genomic instability, apoptosis, and membrane disruption in hepatocytes, while promoting apoptosis and lipid peroxidation in (pre)adipocytes and cytokine production in monocytes (15 studies). In rodent studies (21 studies), GLY/GBH - in doses based on human exposure/toxicological limits - induces inflammatory and oxidative responses in the liver and AT, while causing dysbiosis and metabolic alterations in the gut microbiome axis. In the light of populational-, cell- and animal-based evidence, GLY/GBH disturbs key axis of MASLD pathogenesis and is hypothesized to be associated with its clinical outcomes.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104705"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the interaction between the pesticides chlorantraniliprole and imidacloprid with the ATP-binding cassette transporter G2 (ABCG2): In vitro and in vivo assays","authors":"Esther Blanco-Paniagua ,&nbsp;Indira Álvarez-Fernández ,&nbsp;Laura Álvarez- Fernández ,&nbsp;Alicia Millán-García ,&nbsp;Gracia Merino","doi":"10.1016/j.etap.2025.104709","DOIUrl":"10.1016/j.etap.2025.104709","url":null,"abstract":"<div><div>Pesticides used in agriculture to control pests can also affect non-target animals, such as livestock and humans, through exposure. Pesticide residues found in animal-derived products like milk serve as evidence of exposure in mammals and may pose health risks due to bioaccumulation. Understanding the mechanisms of elimination and toxicity of pesticides is essential to minimize their negative impact. The ATP-binding cassette (ABC) transporter ABCG2 is a membrane protein involved in xenobiotic biodisposition and milk secretion. Our purpose was to evaluate its role in the in vitro transport and secretion into milk of two pesticides: chlorantraniliprole and imidacloprid. Using MDCK–II cells transduced with ABCG2, we found that they are effectively transported by murine, ovine and bovine ABCG2. In vivo studies using wild-type and Abcg2^-/- mice showed that milk concentration of chlorantraniliprole was affected by Abcg2, potentially leading to toxicological effects and contributing to unwanted xenobiotic residues in milk.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104709"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a toxicity pathway from activation of the TLR4/NF-κB/NLRP3 axis to Parkinson's disease-like non-motor symptoms in mice","authors":"Kaidong Wang ,&nbsp;Ge Shi ,&nbsp;Yonghang Li ,&nbsp;Qi Liu ,&nbsp;Qianrong Zhang ,&nbsp;Chenyang Wu ,&nbsp;Yuxuan Jiao ,&nbsp;Yuan Ma ,&nbsp;Ai Qi ,&nbsp;Min Huang","doi":"10.1016/j.etap.2025.104706","DOIUrl":"10.1016/j.etap.2025.104706","url":null,"abstract":"<div><div>Bipyridyl herbicides, like paraquat, are among the most widely used herbicides worldwide. Although the lungs are the main target organ for acute exposure to paraquat, chronic exposure to paraquat is thought to induce neurotoxicity and is one of the environmental risk factors for neurodegenerative diseases. In the last two decades, as paraquat has been banned in some regions, diquat has gradually become its replacement. However, systematic studies of environmental factor-induced neurodegenerative diseases are incomplete, and information on threats and risk assessment is still insufficient. The aim of this study was to investigate the association between bipyridine herbicides and sporadic Parkinson's disease. Among them, we refer to the concept of Adverse Outcome Pathway (AOP), integrate, analyse and propose the toxicity pathway similar to AOP. It provides ideas for us to conduct traditional toxicological studies. As a result, the present study reveals that bipyridine herbicide exposure causes neuronal pyrocytosis in locus coeruleus through activation of the TLR4/NF-κB/NLRP3 axis, resulting in the development of Parkinson's disease-like non-motor symptoms in mice.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104706"},"PeriodicalIF":4.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}