Environmental toxicology and pharmacology最新文献

{"title":"Evaluation of the ivermectin effects on porcine granulosa cell function","authors":"Giuseppina Basini,&nbsp;Fausto Quintavalla,&nbsp;Simona Bussolati,&nbsp;Francesca Grasselli","doi":"10.1016/j.etap.2026.104960","DOIUrl":"10.1016/j.etap.2026.104960","url":null,"abstract":"<div><div>Ivermectin (IVM) is used as antiparasitic drug in veterinary medicine and it is also the only avermectin approved in humans in several parasitic infestations. We examined this substance because potential critical issues associated with the use were highlighted. Moreover, criticisms related to the ecological-environmental sphere, as well as food contamination arise. Nevertheless, current in vitro studies are lacking. This study aimed to investigate the direct impact of IVM on reproductive function using swine granulosa cells as reproductive cell model. The results show that progesterone, ATP and free radicals’ levels significantly increased (p &lt; 0.05). Non-enzymatic defense systems as well as autophagy did not show significant differences. On the contrary, cell proliferation, estradiol levels, and enzymatic defense systems significantly decreased (p &lt; 0.05) after IVM treatment. Since results show that the normal function of these cells is compromised by IVM the normal reproductive functions of the entire organism could be disrupted.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"123 ","pages":"Article 104960"},"PeriodicalIF":4.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium modulates the antioxidative response in green microalga Monoraphidium cf. contortum","authors":"Dubravka Špoljarić Maronić ,&nbsp;Ivna Štolfa Čamagajevac ,&nbsp;Tanja Žuna Pfeiffer ,&nbsp;Ana Pilipović ,&nbsp;Nikolina Sabo ,&nbsp;Nikolina Bek ,&nbsp;Filip Stević ,&nbsp;Ivana Maksimović ,&nbsp;Anita Galir ,&nbsp;Zdenko Lončarić","doi":"10.1016/j.etap.2026.104958","DOIUrl":"10.1016/j.etap.2026.104958","url":null,"abstract":"<div><div>As algae are the main route of selenium (Se) transport from water to other consumers in food webs, the effect of selenite on the total Se content, growth, and antioxidant status of the unicellular green alga <em>Monoraphidium</em> cf. <em>contortum</em> was determined. The basal Se content (&lt;3 mg/kg DW) was lower compared to other green microalgae, but the species showed a high accumulation potential (284.1 mg/kg DW). Despite early Se accumulation and growth inhibition, a significant loss of chlorophylls and carotenoids was observed only after 72 h. The response of antioxidative enzymes corresponded to a lower intensity of lipid peroxidation, despite increased ROS content at lower selenite concentrations. Primarily, selenite increased the activities of ascorbate peroxidase (APX), glutathione reductase, glutathione peroxidase (GPX), and glutathione S-transferase (GST), and inhibited catalase (CAT) in almost all treatments. Higher GST and GPX activities, as well as selenite-induced non-enzymatic glutathione (GSH) oxidation indicated increased GSH depletion.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"123 ","pages":"Article 104958"},"PeriodicalIF":4.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol S toxicity in zebrafish: Endocrine, neurobehavioural, and metabolic disruptions","authors":"A K M Munzurul Hasan ,&nbsp;Som Niyogi ,&nbsp;Douglas P. Chivers","doi":"10.1016/j.etap.2026.104959","DOIUrl":"10.1016/j.etap.2026.104959","url":null,"abstract":"<div><div>Bisphenol S (BPS), increasingly used as a substitute for Bisphenol A, has raised toxicological concern due to its environmental persistence and widespread exposure. Zebrafish (<em>Danio rerio</em>) provides a valuable model for assessing BPS toxicity because of conserved endocrine and neural pathways, rapid development, and well-established behavioural assays. This review synthesizes current evidence on the effects of BPS exposure in zebrafish. Available studies indicate that BPS is associated with alterations in multiple endocrine axes, including the hypothalamic-pituitary-gonadal, thyroid, and interrenal axes, with consequences for reproduction, thyroid hormone signaling, and stress regulation. Neurobehavioural alterations are frequently reported and are linked to oxidative stress, apoptosis, neurotransmitter imbalance, and isotocinergic or vasotocinergic dysregulation. Additional evidence associates BPS exposure with metabolic, cardiovascular, and immune alterations, including hepatic lipid accumulation, glucose dysregulation, vascular dysfunction, impaired myelopoiesis, inflammatory signaling, and gut microbial imbalance. Finally, this review highlights key knowledge gaps for future research.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"123 ","pages":"Article 104959"},"PeriodicalIF":4.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red Aroeira (Schinus terebinthifolia)-based zinc oxide nanoparticles: A green approach to combat fish pathogens","authors":"Cindy C.M. Santos ,&nbsp;Peterson E.G. Paixão ,&nbsp;Juliana O. Meneses ,&nbsp;Joel A.R. Dias ,&nbsp;Amanda S. Carvalho ,&nbsp;Hugo Leandro dos Santos ,&nbsp;Thigna de Carvalho Batista ,&nbsp;Patricia Severino ,&nbsp;Eliana B. Souto ,&nbsp;Juliana C. Cardoso ,&nbsp;Rodrigo Y. Fujimoto","doi":"10.1016/j.etap.2026.104946","DOIUrl":"10.1016/j.etap.2026.104946","url":null,"abstract":"<div><div>Aquaculture production is often linked to improper use of chemotherapeutic agents for pathogen control, leading to adverse effects in organisms and environment. As an alternative over these toxicological agents, in this study we propose the development of zinc oxide (ZnO) nanoparticles using ethanolic extract of red Aroeira (<em>Schinus terebinthifolia</em>) (EERA) and tested them against fish pathogens. Green synthesized ZnO nanoparticles had a mean hydrodynamic diameter of ca. 150 nm, and showed a broad-spectrum antibacterial activity, as well as antifungal and antiparasitic activities against <em>Saprolegnia parasitica</em> and <em>Ichthyophthirius multifiliis</em>, at the highest tested concentrations (10 and 15 mg/mL). The extract alone showed antibacterial activity against <em>Aeromonas hydrophila</em>, but not against <em>Streptococcus agalactiae</em>, suggesting a synergistic effect between EERA and ZnO nanoparticles. These findings demonstrate that green ZnO nanoparticles hold significant potential as a multifunctional antimicrobial agent for managing various pathogens in aquaculture, offering an environmentally friendly alternative to conventional chemotherapeutics.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104946"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146014952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Permethrin decreased insulin secretion and the expression of proteins linked to metabolism and exocytosis in INS832/3 pancreatic beta cells","authors":"Nela Pavlikova,&nbsp;Jan Sramek,&nbsp;Kamila Balusikova,&nbsp;Vlasta Nemcova","doi":"10.1016/j.etap.2026.104952","DOIUrl":"10.1016/j.etap.2026.104952","url":null,"abstract":"<div><div>Permethrin, a widely used pyrethroid insecticide, has been suspected of contributing to metabolic disturbances, insulin resistance, and diabetes, but its effect on pancreatic beta cells has never been investigated. We evaluated the impact of one-week exposure to sublethal concentrations (1 μM and 10 μM) of permethrin on the function and viability of INS832/3 pancreatic beta cells. Permethrin significantly decreased glucose- and KCl-stimulated insulin secretion despite only a partial reduction in intracellular insulin levels. It also altered the expression of proteins involved in glucose metabolism, energy sensing, lipid storage and exocytosis machinery, suggesting a multifaceted impairment of cell functioning. A comparative analysis using HepG2 liver cells revealed tissue-specific responses. This is the first report showing that permethrin exposure decreases insulin secretion by pancreatic beta cells. This data can help to better evaluate the risk assessment of permethrin as a potential endocrine disruptor that may increase the risk of diabetes.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104952"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimetal exposure and its association with neutrophil extracellular traps (NETs) markers and prothrombotic biomarkers in occupational groups","authors":"Ana Karen González Palomo ,&nbsp;Juan Diego Cortés García ,&nbsp;Kelvin Saldaña Villanueva ,&nbsp;José de Jesús Mejía Saavedra ,&nbsp;Fernando Díaz Barriga ,&nbsp;Francisco Javier Pérez Vázquez","doi":"10.1016/j.etap.2026.104955","DOIUrl":"10.1016/j.etap.2026.104955","url":null,"abstract":"<div><div>This study evaluated the association between multimetal exposure and neutrophil extracellular traps (NETs) and prothrombotic biomarkers in quarry workers, brickyard makers, and waste recyclers. A cross-sectional study was conducted in 91 adults. Urinary concentrations of seven metals and serum NETs and prothrombotic markers were quantified. Correlation analysis and PCA were used to identify exposure–response patterns. Waste recyclers had the highest Al and Hg levels, while brick makers showed the highest As and Zn concentrations. NETs biomarkers varied across scenario: neutrophil elastase (NE) and citrullinated histone (H3Cit) were elevated in brick makers, whereas circulating DNA was highest in quarry workers. PCA indicated that Cr–Ni–Al exposure aligned with NETs component scores, and correlations showed positive associations of Hg and As with NE, H3Cit, and myeloperoxidase (MPO). Higher As, Hg, and Zn were also associated with elevated platelets and <span>D</span>-dimer. Overall, multimetal exposure modulated NETs activity and early prothrombotic response.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104955"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles","authors":"Anda R. Gliga ,&nbsp;Sarah McCarrick ,&nbsp;Vilhelm Malmborg ,&nbsp;Pekka Kohonen ,&nbsp;Anastasiia Snigireva ,&nbsp;Brandon Mills ,&nbsp;Pernille Høgh Danielsen ,&nbsp;Lena Palmberg ,&nbsp;Karin Broberg ,&nbsp;Joakim Pagels ,&nbsp;Ulla Vogel","doi":"10.1016/j.etap.2025.104918","DOIUrl":"10.1016/j.etap.2025.104918","url":null,"abstract":"<div><div>Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104918"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145845149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wastewater-based epidemiology of non-steroidal anti-inflammatory drugs in six cities of Spain: Consumption patterns, seasonal trends, and the role of refined correction factors","authors":"Paloma De Oro-Carretero,&nbsp;Natalia Melones-Peña,&nbsp;Emma Gracia-Lor ,&nbsp;Jon Sanz-Landaluze","doi":"10.1016/j.etap.2026.104934","DOIUrl":"10.1016/j.etap.2026.104934","url":null,"abstract":"<div><div>Non-steroidal anti-inflammatory (NSAIDs) pharmaceuticals are one of the most widely used drugs worldwide. Their consumption in Spain experienced an increase from 2020 due to the global pandemic COVID-19, according to prescription packaging sales. However, many of these pharmaceutical products are over-the-counter or not all of the amount sold is consumed. Therefore, wastewater-based epidemiology (WBE) was applied to provide objective and real-time information on their consumption. Seven pharmaceuticals were measured in untreated wastewater collected during one week between December 2020 and December 2021 from seven wastewater treatment plants (WWTPs) in different Spanish cities. The samples were analysed using an analytical methodology based on solid-phase extraction and gas chromatography tandem mass spectrometry with prior derivatization. The concentrations found were converted into population normalized daily consumption considering the daily flow rate, population and correction factors. Refined correction factors have been obtained by a systematic review of all accessible studies, considering the excretion factors of each substance and their stability in the sewer system. The results showed that pharmaceutical consumption was similar in the different WWTPs, with paracetamol, ibuprofen and acetylsalicylic acid being the most consumed with mean values of 7, 41 and 12 g/day/1000 inhabitants, respectively. The estimated NSAID consumption (MD), derived from wastewater analysis, aligned with the dispensing values (DCV), except for ibuprofen and diclofenac showing an overestimation of consumption. Principal component analysis (PCA) revealed the predominant days of the week for each compound. In addition, an increase in consumption was observed in the New Year's Eve week. Overall, these findings demonstrate that WBE provides a reliable and complementary approach for assessing real NSAID consumption patterns in the population.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104934"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145954691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotoxicity and teratogenicity induced by carbendazim and ametryn in zebrafish: Implications for environmental and biological health","authors":"Júlia Robert Teixeira ,&nbsp;Livia Alves de Macedo Tavares ,&nbsp;Ariana Pereira da Silva ,&nbsp;João Vitor de Macedo-Sampaio ,&nbsp;Hugo Alexandre de Oliveira Rocha ,&nbsp;Katia Castanho Scortecci ,&nbsp;Bruno Fiorelini Pereira ,&nbsp;Ana Carolina Luchiari","doi":"10.1016/j.etap.2026.104947","DOIUrl":"10.1016/j.etap.2026.104947","url":null,"abstract":"<div><div>Ametryn (AMT) and Carbendazim (CBZ) are pesticides widely used in agricultural crops to control pests and increase food production efficiency. Despite their toxicological risks and questionable effectiveness, the real impacts of exposure to these compounds and their residues in the environment are still difficult to determine, particularly when lethal, sublethal, biochemical, and behavioral endpoints are not evaluated within the same experimental framework. This study aimed to evaluate the effects of acute exposure to AMT and CBZ in non-target organisms, using zebrafish (<em>Danio rerio</em>) as a model. Zebrafish embryos were exposed from 3 to 120 h post-fertilization to different concentrations of the chemicals: 0.2, 1.0, 2.0, 20, and 40 mg/L of AMT, and 0.07, 0.15, 0.3, 0.5, and 1 µg/L of CBZ (n = 20/group). Mortality, teratogenicity, cardiotoxicity, and neurotoxicity were assessed. High mortality and malformation induction were observed for both pesticides, with estimated LC₅₀ values of 1.33 mg/L for AMT and 0.02 µg/L for CBZ. Both pesticides induced increased heart rate compared to the control group. Furthermore, the pesticides reduced avoidance behavior and elevated reactive oxygen species (ROS) and apoptotic cells at concentrations below LC₅₀, evidencing their neurotoxic effects. These findings highlight the importance of integrating sublethal and neurobehavioral endpoints into ecological risk assessment of pesticide exposure.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104947"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146014953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limited bioconcentration of water-associated pharmaceutical active compounds through short-term exposure in signal crayfish (Pacifastacus leniusculus)","authors":"Anna Koubová ,&nbsp;Worrayanee Thammatorn ,&nbsp;Antonín Kouba ,&nbsp;Vladimír Žlábek ,&nbsp;Bent Speksnijder ,&nbsp;Daniel Cerveny ,&nbsp;Oksana Golovko","doi":"10.1016/j.etap.2026.104935","DOIUrl":"10.1016/j.etap.2026.104935","url":null,"abstract":"<div><div>Pharmaceutical active compounds (PhACs) increasingly appear as complex mixtures in aquatic ecosystems, yet their bioconcentration in non-target organisms is poorly understood. This study examined tissue-specific distribution of five PhACs – bicalutamide, amitriptyline, furosemide, daidzein and sertraline – in signal crayfish (<em>Pacifastacus leniusculus</em>) after 96-hour exposure and an equal depuration period. Crayfish were subjected to environmentally relevant and 10-fold elevated mixture concentrations. Water and tissues (haemolymph, hepatopancreas, muscle) were analysed using ultra-high performance liquid chromatography–tandem mass spectrometry. Despite verified water concentrations and stable conditions, none of the compounds were quantifiable in tissues, except trace sertraline near the detection limit in some controls. The lack of detectable residues indicates minimal bioconcentration, likely due to physicochemical properties (low log <em>K</em>_ow), rapid metabolism and efficient excretion. Results underscore the need for longer exposures and metabolite-focused studies to better assess environmental fate, tissue kinetics and potential risks of PhAC mixtures in freshwater invertebrates.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"122 ","pages":"Article 104935"},"PeriodicalIF":4.2,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145954677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}