Environmental toxicology and pharmacology最新文献

{"title":"Electronic waste leachates: A silent toxicological threat to aquatic life – Insights into biochemical and endocrine disruption mechanism in grass carp (Ctenopharyngodon idella) sub-adults","authors":"Akaninyene Joseph ,&nbsp;Edet Asanga ,&nbsp;Gary Hardiman ,&nbsp;Aaron Schultz ,&nbsp;Robert Niven ,&nbsp;Ochuko Eriegha ,&nbsp;Osarenaye Etinosa-Okankan ,&nbsp;Laraib Saeed ,&nbsp;Kabari Sam ,&nbsp;Victor Eyo","doi":"10.1016/j.etap.2025.104822","DOIUrl":"10.1016/j.etap.2025.104822","url":null,"abstract":"<div><div>Electronic waste (e-waste) leachates pose a growing threat to aquatic ecosystems and public health. This study aimed to evaluate the sub-lethal toxic effects and underlying mechanisms of e-waste leachate exposure in sub-adults of <em>Ctenopharyngodon idella</em>. Sub-adults were exposed for 28 days to environmentally relevant sub-lethal concentrations (0.2, 0.5, 2, and 3 mg/L) of e-waste leachate. The results revealed dose-dependent decreases in testosterone, 17β-estradiol, protein, thyroxine, and tri-iodothyronine, alongside increases in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, cholesterol, and glucose levels compared to control and over the exposure period. Furthermore, histological examination (kidney and liver) indicates cellular damage and loss of structural integrity. Monte Carlo simulation further validated the physiological disruptions and toxic risk associated with e-waste leachate exposure. These findings highlight the stress-mediated toxicity of e-waste leachate in fish, emphasizing the urgent need for effective e-waste management to protect aquatic environments and public health.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104822"},"PeriodicalIF":4.2,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benzophenone-3 (BP3), bisphenol A (BPA), and their combination impair ovarian response to gonadotropin stimulation in a multi-exposure multiparity model.","authors":"Valentina Galliani ,&nbsp;Joana Fessia ,&nbsp;Clarisa Guillermina Santamaría ,&nbsp;Julián Elías Abud ,&nbsp;Horacio Adolfo Rodríguez","doi":"10.1016/j.etap.2025.104821","DOIUrl":"10.1016/j.etap.2025.104821","url":null,"abstract":"<div><div>Despite prevailing evidence that endocrine-disrupting chemicals (EDCs) may exert effects across multiple pregnancies, most studies focus on the first pregnancy Using a multiparity murine model, we evaluated the effects of benzophenone-3 (BP3), bisphenol-A (BPA) or the combination (BP3 +BPA) exposure over two gestational periods (P1 and P2) on pregnancy outcomes and the gonadal function in female offspring from each pregnancy. While maternal weight, litter size, and sex ratio were unaffected, gestation length was altered in BPA and BP3 +BPA groups both in P1 and P2. Ovulation was affected in P2 offspring in all EDC exposed groups. Notably, antral follicle numbers were reduced in all exposed groups, while BPA and BP3 +BPA exposure diminished the primordial follicle reserve in P2 offspring. Estradiol levels were elevated in P2 offspring in the BP3 +BPA group with hCG stimulation. These findings highlight the importance of considering cumulative exposure and exposure to combinations of BP3 and BPA when assessing the long-term reproductive effects of EDCs.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104821"},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity assessment of zinc oxide nanoparticles in the early development of an autochthonous amphibian (Rhinella arenarum)","authors":"Julieta Zarate‑Insúa ,&nbsp;María Celeste Gardey Merino ,&nbsp;Gustavo Pastrana ,&nbsp;Leandro Dufou ,&nbsp;Teresa Fonovich ,&nbsp;Laura Fernández ,&nbsp;Gabriela Svartz","doi":"10.1016/j.etap.2025.104820","DOIUrl":"10.1016/j.etap.2025.104820","url":null,"abstract":"<div><div>The growing use of nanomaterials raises concerns about their environmental release and effects on aquatic ecosystems; however, knowledge about their impacts on amphibians remains scarce. This study evaluated the toxicity of zinc oxide nanoparticles (ZnO-NPs) in early stages of the native amphibian <em>Rhinella arenarum</em> using a multi-biomarker approach. Lethal and sublethal effects, including morphological and behavioral alterations, neurotoxicity, genotoxicity, and disruption of lipid metabolism, were assessed. Larvae were more sensitive in acute exposure (NOEC 96 h: embryos &gt;200 mg/L; larvae 5 mg/L), while embryos showed increased sensitivity in chronic exposure (LC50–504h: embryos 6.66 mg/L; larvae 0.45 mg/L), with gill and movement impairments. Micronucleus tests indicated genotoxicity, and increased butyryl and acetylcholinesterase activity showed neurotoxic effects, both at 5 mg/L. Lipid analysis revealed reduced triglycerides and phospholipids at 0.05 and 0.1 mg/L, suggesting metabolic disruption. These results stress the need to consider multiple endpoints to assess metal-based nanoparticle toxicity in amphibians.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104820"},"PeriodicalIF":4.2,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-effect biomarkers and hazard index approaches to assessing risks from multiple exposure to metals among battery recyclers in France","authors":"Ogier Hanser,&nbsp;Aurélie Martin Remy,&nbsp;Mathieu Melczer,&nbsp;Nathalie Grova,&nbsp;Sophie Ndaw","doi":"10.1016/j.etap.2025.104819","DOIUrl":"10.1016/j.etap.2025.104819","url":null,"abstract":"<div><div>Battery-recycling can expose workers to several metals. This study aims to investigate this multiple exposure, by exploring the potential of effect biomarkers to improve risk assessment for workers, and by calculating a hazard index (HI) to assess the risk of cumulative exposure. Urine samples from 86 workers in three French recycling plants were analysed for metal biomarkers (Cd, Co, Cr, Li, Mn, Ni), kidney-injury biomarkers (α1-microglubulin, retinol binding protein, kidney injury molecule-1, N-acetyl-β-D-gluocosaminidase, albumin, total proteins), and oxidative-stress biomarkers (8-hydroxydeoxyguanosine, malondialdehyde). Kidney-injury biomarker levels successfully differentiated exposed recyclers from administrative workers, while oxidative-stress biomarker levels did not allow this differentiation. The calculation of a HI proved effective in detecting the risk associated with multiple metal exposure, offering a better risk assessment than when considering metals individually. This dual approach, combining HI calculation and analyzing kidney-injury biomarkers, should be strongly considered in the risk assessment of workers exposed to multiple metals.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104819"},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative effects of warming and contaminants on the physiology and behaviour of coldwater stenotherm caddisflies","authors":"Filip Ložek ,&nbsp;Iva Kokotović ,&nbsp;Antonia Smolić ,&nbsp;Luka Bočkor ,&nbsp;Marko Rožman ,&nbsp;Ana Previšić","doi":"10.1016/j.etap.2025.104818","DOIUrl":"10.1016/j.etap.2025.104818","url":null,"abstract":"<div><div>Aquatic ecosystems globally are increasingly affected by both natural and anthropogenic stressors. High-altitude systems are particularly vulnerable due to high specialization of organisms to survive specific conditions, making them especially sensitive to environmental changes related to rising temperatures and chemical pollution from runoff and wastewater discharge. These stressors can significantly alter the physiology, behaviour, and population dynamics of aquatic organisms. Our study investigated the individual and combined impacts of emerging contaminants and elevated water temperature (as a proxy for climate change) on the metabolome, body weight, and behaviour of cold-water stenotherm caddisfly larvae, <em>Drusus croaticus</em>. We conducted a 21-day factorial microcosm experiment with four treatments: control, emerging contaminants (ECs – 8 pharmaceuticals and 1 industrial chemical), elevated temperature (+4°C), and a multiple stressor treatment combining both warming and contaminants. Our results showed that the metabolome of <em>D. croaticus</em> larvae was primarily affected by elevated temperature and the combination of warming and contaminants, reflecting significant metabolic stress. Larvae in the elevated temperature and multiple stressor treatments exhibited significantly higher locomotor activity, moving more than controls. This increased activity appears to have contributed to a marked decrease in body weight compared to controls and larvae exposed to contaminants alone. These findings suggest a trade-off between activity and growth under stress conditions, where increased energy expenditure on movement may come at the cost of reduced growth. More specifically, elevated temperature inhibited growth and triggered hyperactivity. Additionally, the correlation between metabolomic shifts and behavioural changes indicates that metabolic responses to stress develop early, while behavioural effects may take longer to manifest. Our findings give important insights into how multiple stressors affect stenotherm caddisfly species, which are crucial for maintaining the integrity of both aquatic and terrestrial food webs in higher altitude environments. Hence, they underscore the urgent need for conservation measures to protect biodiversity in vulnerable ecosystems impacted by warming and pollution.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104818"},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and toxic effects of the neonicotinoid pesticide thiacloprid in the planarian Girardia dorotocephala","authors":"Sofía G. Martínez-Villalpando,&nbsp;Emily Snyder,&nbsp;Nevy Harris,&nbsp;Megan Fork,&nbsp;Gregory Turner,&nbsp;Oné R. Pagán","doi":"10.1016/j.etap.2025.104816","DOIUrl":"10.1016/j.etap.2025.104816","url":null,"abstract":"<div><div>Neonicotinoids were developed in the 1990s and are among the most popular pesticides worldwide. However, a significant fraction of neonicotinoids end up in freshwater, affecting non-target organisms, including planarians, a type of free-living flatworm. In this work, we explored the effect of the neonicotinoid thiacloprid on planarian behavior. Thiacloprid decreased planarian motility reversibly and increased seizure-like movements (paroxysm-like movements different from the normal gliding behavior of the worms) in a concentration-dependent manner but did not influence planarian environmental light/dark preference at sub-µM concentrations. Exposure to up to 10 µM thiacloprid for 24 h did not cause lethality but markedly decreased motility and caused a concentration-dependent incidence of a “wrinkled” morphology at rest. The quantification of thiacloprid’s behavioral effects reported in the present work allows us to suggest strategies for screening possible alleviators of neonicotinoid effects in planarians and potentially other non-target species. To the best of our knowledge, this is the first report on the effect of thiacloprid on planarians.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104816"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145042737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-steroidal anti-inflammatory drugs (NSAIDs) as a hidden threat to scavenging raptors beyond Gyps: A call for wider research and surveillance","authors":"Kane Colston ,&nbsp;Karen Mifsud ,&nbsp;Nicola Rooney ,&nbsp;Juan Manuel Grande ,&nbsp;Irene Bueno","doi":"10.1016/j.etap.2025.104817","DOIUrl":"10.1016/j.etap.2025.104817","url":null,"abstract":"<div><div>Asian vulture declines have been linked to the consumption of contaminated livestock carcasses with the non-steroidal anti-inflammatory (NSAID) diclofenac. Studies have suggested that the toxicity of NSAIDs to Old World vultures may be through an intronic premature termination codon (PTC) in the gene CYP2C19, encoding a cytochrome P450 enzyme thought responsible for diclofenac metabolism in vultures. However, it remains unclear whether this mechanism applies for all susceptible raptor species. We used nucleotide databases (NCBI) to compare the Cape vulture (<em>Gyps coprotheres</em>) CYP2C19 sequence to other vulture sequences to identify the presence of the intronic PTC. Our search revealed that only <em>Gyps</em> species possessed the CYP2C19 PTC. This is despite NSAID toxicity at similar contaminant levels found in non-<em>Gyps</em> accipitrid mortalities. Our findings suggest avian scavengers could be affected by additional toxicological mechanisms, including sublethal toxicity. Further research is required to establish such mechanisms and exposure risk in non-<em>Gyps</em> scavengers.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104817"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of cell death and genotoxic potential of glyphosate and cypermethrin formulations, individually and in combination, in HEp-2 cells","authors":"Isis Coalova ,&nbsp;Nancy Andrioli ,&nbsp;Hugo March ,&nbsp;María del Carmen Ríos de Molina ,&nbsp;Gabriela Chaufan","doi":"10.1016/j.etap.2025.104815","DOIUrl":"10.1016/j.etap.2025.104815","url":null,"abstract":"<div><div>The increasing global use of pesticides in crop pest control has raised concerns about toxic interactions. This study investigates the interaction between glyphosate and cypermethrin formulations concerning cell death and genotoxicity. Three models were applied to assess whether the combined effects were additive, antagonistic, or synergistic: \"Linear Interaction Effect\", \"Combination Subthresholding,\" and \"Cooperative Effect\". Key indicators, such as apoptotic nuclei, caspase 3/7 activity, early and late apoptosis, micronuclei frequency, and mitotic abnormalities, were evaluated. Results showed synergistic effects on early and late apoptosis, as indicated by the \"Linear Interaction Effect\" model, while morphological apoptosis markers and micronuclei frequency displayed additive effects. Caspase 3/7 activity induction was synergistic, though no interaction was observed in the combination effect. These findings suggest that agrochemical mixtures cause more severe toxic effects than individual chemicals, emphasizing the need for mechanistic studies on common environmental contaminant mixtures.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104815"},"PeriodicalIF":4.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145002234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-biomarker assessment of essential and toxic elements accumulation in Labeo gonius from Khushab Lake (Pakistan)","authors":"Muhammad Kashif Ashraf ,&nbsp;Sana Ullah ,&nbsp;Muhamamd Bilal ,&nbsp;Cristiana Roberta Multisanti ,&nbsp;Caterina Faggio","doi":"10.1016/j.etap.2025.104814","DOIUrl":"10.1016/j.etap.2025.104814","url":null,"abstract":"<div><div>The continuous increase in the concentration of heavy metals in bodies of water due to human activity poses a serious threat to the sustainability and overall health of the ecosystem. This study investigated the essential and toxic elements bioaccumulation in various tissues (gills, muscles, liver, and brain) of the Minor carp (<em>Labeo gonius)</em> from two sampling sites of highly polluted Khushab Lake, a tributary of River Jhelum (in the Pakistan's Punjab province), along with a less exposed reference site (Chashma Lake-less polluted site). The impacts of pollution on the health profile of the fish were assessed using multiple biomarkers for analysis. The maximum accumulation of essential and toxic elements was reported in liver tissues, followed by gills. Copper (Cu) and iron (Fe) were the highest accumulated essential elements in liver and gills tissues, while magnesium (Mg) and calcium (Ca) were the highest accumulated in muscles and brain tissues. Hematological and serum biochemical profile of <em>L. gonius</em> showed intense abnormalities at Khushab Lake as compared to reference site. Histopathological investigations indicated severe alterations in the liver tissues along with significant changes in other tissues. Principal component analysis (PCA) showed strong correlations among essential and toxic elements in various tissues of fish. The results revealed that aquatic life in Lake Khushab is at the greatest risk to its health. Therefore, mitigating measures should be adapted by key stakeholders including environmental protection agency (ensure disposal of pre-treated wastes and effluents) and fisheries department (limnological assessment and stocking of fish at regular intervals).</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104814"},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transcriptomics-based analysis provides insights into the sex-dependent reproductive toxicity of DEHP in zebrafish (Danio rerio)","authors":"Shirui Liu ,&nbsp;Simeng Yu ,&nbsp;Yankun Huang ,&nbsp;Jingyun Zhang ,&nbsp;Kai Song ,&nbsp;Shenbao Qu ,&nbsp;Cixin Li ,&nbsp;Aijiang Yang ,&nbsp;Xia Hu","doi":"10.1016/j.etap.2025.104810","DOIUrl":"10.1016/j.etap.2025.104810","url":null,"abstract":"<div><div>Di-(2-ethylhexyl) phthalate (DEHP) threatens human and wildlife health seriously, yet sex-dependent reproductive toxicity remains unclear. Therefore, in this study, we exposed zebrafish to various concentrations (0.01, 0.1, 1.0, and 2.0 mg/L) of DEHP for 28 days. Results indicate that DEHP exposure induced oxidative stress as a common response in both sexes and more pronounced reproductive toxicity to males than females. In males, sperm development stagnated at spermatogonia and enriched pathways were mainly associated with ribosome (<em>Rps3</em>, <em>Rps27a</em>) and endoplasmic reticulum stress(<em>Hspa5</em>). In females, oocyte development was affected at later stages and the pathways were mainly lipid metabolism and GnRH signaling pathway. Hub genes from PPI in females were <em>Cacna2d3, Prkcba, Calm2a, Pla2g4aa</em> in SCvsHigh while <em>Pla2g4aa</em> in SCvsLow. Overall, these findings provide new insights into the molecular mechanisms of DEHP toxicity, which is crucial for understanding its impact on reproductive health and ecological risk assessment.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"119 ","pages":"Article 104810"},"PeriodicalIF":4.2,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144926242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}