Environmental toxicology and pharmacology最新文献_第2页

Ammonia-induced testicular tissue damage: Apoptosis and autophagy pathways mediated by regulating Cyt C/Bcl-2 and p62/LC3B pathways 氨诱导睾丸组织损伤：通过调节Cyt C/Bcl-2和p62/LC3B途径介导的凋亡和自噬途径

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-15 DOI: 10.1016/j.etap.2025.104716

Mengqi Duan , Shixiong Dong , Kejun Wang , Peng Shang , Chunli Li

{"title":"Ammonia-induced testicular tissue damage: Apoptosis and autophagy pathways mediated by regulating Cyt C/Bcl-2 and p62/LC3B pathways","authors":"Mengqi Duan , Shixiong Dong , Kejun Wang , Peng Shang , Chunli Li","doi":"10.1016/j.etap.2025.104716","DOIUrl":"10.1016/j.etap.2025.104716","url":null,"abstract":"<div><div>Ammonia gas (NH<sub>3</sub>) is a major hazard in animal husbandry that negatively affects animal growth and reproductive activities. Although the adverse effects of ammonia on livestock health and performance have been studied, its specific effects on the male reproductive system and its underlying biological mechanisms remain unclear. The aim of this study was to investigate the effects of ammonia exposure on male reproduction and its potential mechanisms of action. Immunohistochemistry revealed a significant increase in IL-10 expression in mouse testicular tissues under ammonia-induced stress. Ammonia exposure significantly decreased anti-apoptotic factor Bcl-2 and anti-autophagy factor p62 expression, coupled with increased expression of pro-apoptotic factors, including p53, Caspase-8, Bax, and Apaf, as well as the pro-autophagy factors Beclin1, ATG5, LC3B, the ratio of Bax/Bcl-2 and the ratio of P53/Bcl-2. The fluorescence intensity of the Cyt C apoptotic gene and pro-autophagy factor LC3B in immune tissues was significantly higher than that in the normal control group. In summary, ammonia exposure significantly affects the reproductive ability of male mice by inducing inflammation, apoptosis, and autophagic damage in the testes. Our findings provide insights into ammonia-induced reproductive disorders, which will contribute to the management and control of ammonia in animal husbandry, thereby reducing the negative effects of ammonia on the reproductive health of livestock and poultry and improving the quality and yield of livestock products.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104716"},"PeriodicalIF":4.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144096435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time dynamics models for oxidative stress markers in honey bees (Apis mellifera) following paraquat-induced stress 百草枯诱导应激后蜜蜂氧化应激标志物的时间动力学模型

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-15 DOI: 10.1016/j.etap.2025.104718

Jana Biová , Dalibor Kodrík , Martina Janků , Silvie Dostálková , Michael Simone-Finstrom , Marek Petřivalský , Jiří Danihlík

{"title":"Time dynamics models for oxidative stress markers in honey bees (Apis mellifera) following paraquat-induced stress","authors":"Jana Biová , Dalibor Kodrík , Martina Janků , Silvie Dostálková , Michael Simone-Finstrom , Marek Petřivalský , Jiří Danihlík","doi":"10.1016/j.etap.2025.104718","DOIUrl":"10.1016/j.etap.2025.104718","url":null,"abstract":"<div><div>Many agrochemicals disrupt redox homeostasis, yet the dynamics of oxidative stress responses in honey bees (<em>Apis mellifera</em>) remain insufficiently understood. This study established a controlled model to monitor ROS-related markers over time following paraquat injection, using Bayesian modeling to characterize time-dependent changes. We observed a transient rise in hydroperoxides and early fluctuation in adipokinetic hormone (AKH) levels, which declined and stabilized within 8 hours. No significant differences were detected in secondary lipid peroxidation products (TBARS) among treatments. While injection does not represent natural exposure pathways, it enables precise dosing and timing, avoiding variability from oral intake. This experimental design provides a tractable system to investigate oxidative stress mechanisms under defined conditions. Our findings underscore the importance of time-resolved analysis in redox physiology and offer a mechanistic framework to complement field-relevant toxicological studies in bees and other beneficial insects.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104718"},"PeriodicalIF":4.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The blood response to subway-derived iron nanoparticles 血液对地铁铁纳米颗粒的反应。

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-14 DOI: 10.1016/j.etap.2025.104717

Kristina N. Ekdahl , Lisa Monie , Ana T. Juárez-Facio , Ulf Olofsson , Alessandro Mancini , Bozhena Tsyupa , Karine Elihn , Bo Nilsson , Karin Fromell

{"title":"The blood response to subway-derived iron nanoparticles","authors":"Kristina N. Ekdahl , Lisa Monie , Ana T. Juárez-Facio , Ulf Olofsson , Alessandro Mancini , Bozhena Tsyupa , Karine Elihn , Bo Nilsson , Karin Fromell","doi":"10.1016/j.etap.2025.104717","DOIUrl":"10.1016/j.etap.2025.104717","url":null,"abstract":"<div><div>In this study, we investigated the impact of iron-rich nanoparticles derived from different locations in the subway on the innate immune system in blood. Nanoparticles were generated from Third Rail, Rail, and Wheel materials and characterized using several techniques. The response in a human whole-blood model was analyzed using ELISA and capillary immunoelectrophoresis. All nanoparticles were iron oxides, but Third Rail nanoparticles also contained Silicon and were highly thrombo-inflammatory, activating Factor XI-induced coagulation and pro-inflammatory kallikrein/kinin pathways. Wheel and Rail nanoparticles were less reactive, mainly activating the kallikrein/kinin pathway, leading to milder inflammatory reactions. The strong thrombo-inflammatory properties of Third Rail nanoparticles are attributed to their high Silicon content. None of the nanoparticles significantly activated the complement system. In conclusion, we found that the elemental composition of nanoparticles is crucial in determining whether activation leads to kallikrein/kinin system activation and bradykinin release or Factor XI activation and thrombosis.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104717"},"PeriodicalIF":4.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144087104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of genetic damage levels in agricultural workers exposed to pesticides in Paraíba, Brazil 巴西Paraíba接触农药的农业工人遗传损害水平评估

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-09 DOI: 10.1016/j.etap.2025.104715

Laís Campos Teixeira de Carvalho-Gonçalves , Patricianny Barreto de Góis Santos , Glauciane Andrade da Silva , Lucas Monteiro Bezerra Pinheiro , Thiago Marcel Galdino Olinto , Adegildo Rolim de Abreu Junior , Márcia Helena Pontieri , Solange Maria de Vasconcelos , Jéssica Pedrosa de Lima , Luiz Gualberto de Andrade Sobrinho , Angela Lima Meneses de Queiroz , Márcia Aparecida Cezar , Juan Carlos Ramos Gonçalves

{"title":"Assessment of genetic damage levels in agricultural workers exposed to pesticides in Paraíba, Brazil","authors":"Laís Campos Teixeira de Carvalho-Gonçalves , Patricianny Barreto de Góis Santos , Glauciane Andrade da Silva , Lucas Monteiro Bezerra Pinheiro , Thiago Marcel Galdino Olinto , Adegildo Rolim de Abreu Junior , Márcia Helena Pontieri , Solange Maria de Vasconcelos , Jéssica Pedrosa de Lima , Luiz Gualberto de Andrade Sobrinho , Angela Lima Meneses de Queiroz , Márcia Aparecida Cezar , Juan Carlos Ramos Gonçalves","doi":"10.1016/j.etap.2025.104715","DOIUrl":"10.1016/j.etap.2025.104715","url":null,"abstract":"<div><div>Pesticide use has transformed agriculture, but improper handling poses serious health and environmental risks. Brazil, a top global pesticide consumer, faces alarming unapproved usage—nearly 80 % of pesticides sold lack approval in at least three OECD countries. This study investigates genotoxic effects on farmers in Paraíba, Brazil, analyzing buccal mucosa cells for DNA and cellular damage. Using the Buccal Micronucleus Cytome (BMCyt) assay and comet assay, we compared 33 pesticide-exposed farmers to 29 controls. The findings revealed significantly higher frequencies of cellular alterations and DNA damage among exposed farmers relative to the control group, with no significant impact from factors such as smoking, alcohol consumption, or family cancer history. These results underscore the genotoxic risks linked to prolonged pesticide exposure and highlight the necessity for stricter regulatory measures as well as the need for further large-scale studies to mitigate health risks within agricultural communities.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104715"},"PeriodicalIF":4.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of PDYN 68-bpVNTR, BDNF rs6265, OPRD1 rs569356 and OPRM1 rs2075572 polymorphisms on opioid-methamphetamine co-use PDYN 68-bpVNTR、BDNF rs6265、OPRD1 rss569356和OPRM1 rs2075572多态性对阿片类药物与甲基苯丙胺共同使用的影响

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-08 DOI: 10.1016/j.etap.2025.104714

Merve Ak , Gizem Özer-Uzaldı , Mukaddes Asena Yıldırım , Selin Özkan-Kotiloğlu , Mustafa Danışman , Dilek Kaya-Akyüzlü

{"title":"Effects of PDYN 68-bpVNTR, BDNF rs6265, OPRD1 rs569356 and OPRM1 rs2075572 polymorphisms on opioid-methamphetamine co-use","authors":"Merve Ak , Gizem Özer-Uzaldı , Mukaddes Asena Yıldırım , Selin Özkan-Kotiloğlu , Mustafa Danışman , Dilek Kaya-Akyüzlü","doi":"10.1016/j.etap.2025.104714","DOIUrl":"10.1016/j.etap.2025.104714","url":null,"abstract":"<div><div>To investigate the impact of <em>PDYN</em> 68-bpVNTR, <em>BDNF</em> rs6265, <em>OPRD1</em> rs569356 and <em>OPRM1</em> rs2075572 variations on the susceptibility to opioid-methamphetamine co-use, a total of 532 individuals, including opioid (n = 104), and methamphetamine (n = 166) users as well as opioid and methamphetamine co- users (n = 158) and healthy individuals (n = 104), were included. <em>BDNF</em> rs6265, <em>OPRD1</em> rs569356 and <em>OPRM1</em> rs2075572 were genotyped by PCR-RFLP method, while <em>PDYN</em> 68-bp VNTR was genotyped by PCR. The assessment of impulsiveness, craving, withdrawal, anxiety and depressive symptoms was conducted using scales. A significant difference was identified between the substance-using groups and the control group in view of the alleles of <em>PDYN</em> 68-bp VNTR (p = 0.001). The allele frequencies of the <em>PDYN</em> 68-bp VNTR exhibited a difference between opioid and methamphetamine users (p = 0.018). The 5-repeat allele was first observed in the Turkish population. The analysed polymorphisms could contribute to the development of addiction, exerting an influence on impulsiveness, craving and withdrawal.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104714"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined exposure to atrazine and phoxim exacerbated the alterations of enzyme activity and abnormal gene expression in earthworms (Eisenia fetida) 阿特拉津和硫辛硫磷联合暴露加剧了蚯蚓酶活性的改变和基因的异常表达。

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-07 DOI: 10.1016/j.etap.2025.104713

Jinzhan Li , Jingwen Wang , Fang Shu , Nan Huang , Ru Jia , Yanhua Wang

{"title":"Combined exposure to atrazine and phoxim exacerbated the alterations of enzyme activity and abnormal gene expression in earthworms (Eisenia fetida)","authors":"Jinzhan Li , Jingwen Wang , Fang Shu , Nan Huang , Ru Jia , Yanhua Wang","doi":"10.1016/j.etap.2025.104713","DOIUrl":"10.1016/j.etap.2025.104713","url":null,"abstract":"<div><div>Multiple pesticides often coexist in soil, potentially leading to interactions among their components, these may detrimentally impact soil organisms. This study assessed the potential risks posed by simultaneous exposure to atrazine (ATR) and phoxim (PHO) on enzyme and transcription levels in earthworms (<em>Eisenia fetida</em>). The results revealed that ATR exhibited higher acute toxicity towards <em>E. fetida</em> compared to PHO, and their combined exposure resulted in a synergistic acute effect. Furthermore, low concentration combined exposure significantly stimulated catalase (CAT), malondialdehyde (MDA), and total superoxide dismutase (T-SOD) activities, which lead to more severe oxidative damage. Elevated expression levels of translationally controlled tumor protein (<em>tctp</em>) and calreticulin (<em>crt</em>) genes were observed in most exposed groups compared to the control. The synergistic effects of ATR and PHO on earthworms observed in this study may pose ecological risks to the soil ecosystem; thus, more attention should be paid to the joint effects of different pesticides.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104713"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term joint exposure of outdoor air pollutants and impaired kidney function in Korean adults: A mixture analysis based on a nationwide sample (2007–2019) 韩国成年人长期暴露于室外空气污染物和肾功能受损：基于全国样本的混合分析（2007-2019）

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-05-06 DOI: 10.1016/j.etap.2025.104712

Seong-Uk Baek , Jin-Ha Yoon

{"title":"Long-term joint exposure of outdoor air pollutants and impaired kidney function in Korean adults: A mixture analysis based on a nationwide sample (2007–2019)","authors":"Seong-Uk Baek , Jin-Ha Yoon","doi":"10.1016/j.etap.2025.104712","DOIUrl":"10.1016/j.etap.2025.104712","url":null,"abstract":"<div><div>We explored the association between exposure to air pollutant mixture and renal function. A nationwide sample of 69,066 Korean adults was analyzed. The 1-year moving averages of the concentrations of PM<sub>10</sub>, PM<sub>2.5</sub>, SO₂, NO₂, CO, and O₃ were estimated using the air pollution modeling. The outcome was estimated glomerular filtration rate (eGFR) value. Quantile-based G-computation analyses were performed to determine how a simultaneous increase in the air pollutant mixture by one quartile was associated with changes in eGFR levels. The mean of eGFR was 88.9 among the sample. In the quantile-based G-computation model, one-quartile increase in the levels of air pollutant mixture was linked to a decrease in eGFR by −0.85 (95 % CI: −1.14, −0.57), with CO and PM<sub>2.5</sub> contributing 52.3 % and 37.3 %, respectively, to the negative association between the pollutant mixture and eGFR. Our results suggest that long-term exposure to air pollutant mixtures is linked to impaired renal function.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104712"},"PeriodicalIF":4.2,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tebuconazole-based fungicide impairs embryonic development of the South African Clawed Frog Xenopus laevis 以替布康唑为基础的杀菌剂损害南非爪蟾的胚胎发育

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-04-30 DOI: 10.1016/j.etap.2025.104708

Vanessa Ihle , Hannah Flach , Florian Kaminski, Petra Dietmann, Sarah Pfeffer, Susanne J. Kühl

{"title":"Tebuconazole-based fungicide impairs embryonic development of the South African Clawed Frog Xenopus laevis","authors":"Vanessa Ihle , Hannah Flach , Florian Kaminski, Petra Dietmann, Sarah Pfeffer, Susanne J. Kühl","doi":"10.1016/j.etap.2025.104708","DOIUrl":"10.1016/j.etap.2025.104708","url":null,"abstract":"<div><div>Tebuconazole (TBZ) is a broad-spectrum fungicide that disrupts fungal cell membrane. Due to its extensive application in agriculture, TBZ is frequently detected in aquatic ecosystems, posing potential risks to amphibians. However, the effects of TBZ-based formulations on amphibian development remain poorly understood. The present study investigates the effects of the TBZ-based fungicide Tebucur on the embryogenesis of the South African clawed frog. Two-cell stage embryos were exposed to varying concentrations of Tebucur (0.01–100 mg a.i./L) in a static non-renewal bioassay. The exposure time varied between four and 14 days, depending on the desired developmental stage of the embryos and the analysis method. Tebucur exposure resulted in mortality with a LC50-value of 8.0 mg a.i./L at 14 days. As well, various morphological abnormalities during neural and cardiac development emerged. Additionally, a reduction of overall mobility was observed. This underlines the need for strict regulation of the use of TBZ-formulations.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104708"},"PeriodicalIF":4.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pesticide stress induces spermatozoa DNA damage and morphological abnormalities in Apis mellifera populations 农药胁迫诱导蜜蜂种群精子DNA损伤和形态异常

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-04-30 DOI: 10.1016/j.etap.2025.104710

Vesela Mitkovska , Ivan Stoyanov , Tsenka Chassovnikarova , Penka Vasileva , Plamen Petrov , Evgeniya N. Ivanova

{"title":"Pesticide stress induces spermatozoa DNA damage and morphological abnormalities in Apis mellifera populations","authors":"Vesela Mitkovska , Ivan Stoyanov , Tsenka Chassovnikarova , Penka Vasileva , Plamen Petrov , Evgeniya N. Ivanova","doi":"10.1016/j.etap.2025.104710","DOIUrl":"10.1016/j.etap.2025.104710","url":null,"abstract":"<div><div>The rising losses of honey bee colonies are causing public concern regarding the species' long-term sustainability. Therefore, this study aimed to assess how pesticide exposure affects DNA damage and morphological traits of drone spermatozoa across different <em>Apis mellifera</em> populations, categorized by varying colony loss rates. Chromatographic analysis identified 24 pesticide residues in bee samples and colony food stocks. Significant differences were found in comet assay parameters (tail intensity and Olive tail moment), frequency of morphological abnormalities, and morphometric variations in spermatozoa between apiaries experiencing high versus low mortality rates. The findings indicate that DNA damage and morphological abnormalities in spermatozoa, potentially caused by pesticide stress, compromise the reproductive ability of honey bee drones, contributing to the complex phenomenon of colony loss. The comet assay parameters and morphological aberrations of honey bee spermatozoa may serve as effective biomarkers for evaluating the fitness of drone spermatozoa and assessing environmental hazards to bee populations.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104710"},"PeriodicalIF":4.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic and metabolic effects of organic extracts from airborne fine particulate matter (PM2.5) in neuronal cells 空气中细颗粒物（PM2.5）有机提取物对神经元细胞的细胞毒性和代谢作用

IF 4.2 3区环境科学与生态学

Environmental toxicology and pharmacology Pub Date : 2025-04-29 DOI: 10.1016/j.etap.2025.104707

Tatiana Silva , Célia Alves , Margarita G. Evtyugina , Ana S. Dias , Guilherme Martins Pereira , Pérola de Castro Vasconcellos , Maria de Fátima Andrade , Helena Oliveira , Iola F. Duarte

{"title":"Cytotoxic and metabolic effects of organic extracts from airborne fine particulate matter (PM2.5) in neuronal cells","authors":"Tatiana Silva , Célia Alves , Margarita G. Evtyugina , Ana S. Dias , Guilherme Martins Pereira , Pérola de Castro Vasconcellos , Maria de Fátima Andrade , Helena Oliveira , Iola F. Duarte","doi":"10.1016/j.etap.2025.104707","DOIUrl":"10.1016/j.etap.2025.104707","url":null,"abstract":"<div><div>Airborne fine particulate matter (PM<sub>2.5</sub>) has been linked to neurological diseases, but its cellular and metabolic effects remain incompletely understood. This study assessed the cytotoxic and metabolic impact of PM<sub>2.5</sub> samples from São Paulo, Brazil, on SH-SY5Y neuroblastoma cells. Even at low toxicity levels (IC<sub>10</sub>–IC<sub>30</sub>), PM<sub>2.5</sub> organic extracts induced apoptosis, increased TNF-α secretion, and triggered moderate oxidative responses. Metabolomic analyses revealed a downregulation of energy-producing pathways, including glycolysis and the TCA cycle, along with decreased ATP and phosphocreatine levels. Compensatory adaptations were evident, such as increased proline oxidation, lipid accumulation, and activation of the creatine-phosphocreatine system. One-carbon metabolism was also affected, with changes suggesting suppression of the folate and methionine cycles. Elevated glutathione levels indicated an enhanced antioxidant response. These findings highlight how PM<sub>2.5</sub> disrupts neuronal energy homeostasis and redox balance, offering new insights into the cellular mechanisms of air pollution-related neurotoxicity.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"116 ","pages":"Article 104707"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0