Biomarker-based assessment of sublethal metal exposure in brown trout and parasites acanthocephalans from a protected karst river

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-10-01 DOI:10.1016/j.etap.2025.104823

Sara Šariri , Tatjana Mijošek Pavin , Zuzana Redžović , Zoran Kiralj , Dušica Ivanković , Vlatka Filipović Marijić

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引用次数: 0

Abstract

Karst freshwater ecosystems are especially susceptible to metal pollution, even in protected areas. Present study assessed biomarker responses in intestine of Salmo trutta and their intestinal parasites acanthocephalans at three sites along the karst Krka River: river source, a wastewater-impacted site, and a site within the Krka National Park. Biomarkers of metal exposure, general stress, lipid peroxidation, antioxidant defense, and tissue metabolic activity were analyzed in relation to season, fish biometry, cytosolic metal(loid) concentrations, and parasite burden. Fish from both the wastewater-impacted site (KRK) and the national park site (KNP) showed moderate stress and significantly elevated lipid peroxidation compared to the river source. Correlation analysis revealed stronger biomarker–metal associations in spring, and higher stress in smaller fish with lower parasite burdens. This study provides rare data on biomarker responses in fish intestine and the first data on metallothionein and cytosolic protein levels in acanthocephalans, which exhibited higher biomarker values than host tissue. These results support biomarker use for early detection of sublethal contamination.

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喀斯特保护河流中褐鳟和棘头类寄生虫亚致死金属暴露的生物标志物评价。

喀斯特淡水生态系统尤其容易受到金属污染的影响，即使在保护区也是如此。本研究评估了喀斯特克尔卡河沿岸3个地点（河流源头、污水影响地点和克尔卡国家公园内地点）鲑及其肠道寄生虫棘头类肠道生物标志物的反应。分析了金属暴露、一般应激、脂质过氧化、抗氧化防御和组织代谢活性的生物标志物与季节、鱼类生物计量、细胞质金属（样蛋白）浓度和寄生虫负担的关系。与河流源相比，来自污水影响区（KRK）和国家公园区（KNP）的鱼类均表现出中度应激和显著升高的脂质过氧化。相关分析显示，生物标志物与金属在春季的相关性较强，且对体型较小、寄生虫负荷较低的鱼类的应激较高。该研究提供了鱼类肠道生物标志物应答的罕见数据，以及棘头动物中金属硫蛋白和胞质蛋白水平的首次数据，这些生物标志物的价值高于宿主组织。这些结果支持将生物标志物用于亚致死污染的早期检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.