Developmental and caste-specific expression patterns of ATP-binding cassette (ABC) transporters in honey bees (Apis mellifera)

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-08-10 DOI:10.1016/j.etap.2025.104789

Angela M. Encerrado-Manriquez , Zeke T. Spooner , Tina T. Truong , Julia D. Fine , Sascha C.T. Nicklisch

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Abstract

While honey bees play a vital role in global crop production, they face increasing exposure to xenobiotic chemicals during commercial pollination. Multidrug-resistance (MDR)-type ATP-binding cassette (ABC) transporters provide the first line of defense against xenobiotic chemicals. This study investigated the gene expression profiles of 12 ABC transporters involved in chemical detoxification across three honey bee castes and 13 life stages using quantitative real-time PCR. Six ABC genes showed increased expression during worker bee development and were identified as MDR-like transporters (Ame-ABCB1, Ame-ABCB6, Ame-ABCC4a-c, Ame-ABCG1). Four transporters showed pupal-specific expression during metamorphosis. Queens exhibited significantly reduced MDR transporter expression compared to workers between 1.7-fold lower (ABCB6) and 17.5-fold lower (ABCB1). Drones showed intermediate expression levels. Queen ovaries demonstrated tissue-specific upregulation of select transporters. These findings reveal a vulnerability hierarchy (foragers > drones > queens) and suggest caste-specific trade-offs between reproduction and chemical defense in honey bee superorganisms.

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蜜蜂atp结合盒（ABC）转运体的发育和种姓特异性表达模式[j]。

虽然蜜蜂在全球作物生产中发挥着至关重要的作用，但在商业授粉过程中，它们面临着越来越多的外来化学物质。多药耐药（MDR）型atp结合盒（ABC）转运体是抵御外源化学物质的第一道防线。本研究利用实时荧光定量PCR技术研究了三种蜜蜂种姓和13个生命阶段参与化学解毒的12种ABC转运蛋白的基因表达谱。六个ABC基因在工蜂发育过程中表达增加，并被鉴定为耐多药样转运体（Ame-ABCB1, Ame-ABCB6, Ame-ABCC4a-c, Ame-ABCG1）。四种转运蛋白在蛹期特异表达。与工人相比，皇后的MDR转运蛋白表达显著降低，ABCB6低1.7倍，ABCB1低17.5倍。无人机表现出中等水平的表达。蜂王卵巢表现出组织特异性的转运蛋白上调。这些发现揭示了一个脆弱的等级（觅食者，雄蜂，蜂王），并表明在蜜蜂的超级有机体中，繁殖和化学防御之间存在着特定种姓的权衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.