Genetic damage assessment in buccal epithelium cells of plastic greenhouse workers exposed to different pesticides: A cytogenetic biomonitoring study in Mersin, Turkiye

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-08-12 DOI:10.1016/j.etap.2025.104792

Ayla ÇELİK , Emir Han ALTUN , Zuleykha HAJIYEVA , Aysun GÖKMEN , Gizem BARUT

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引用次数: 0

Abstract

Pesticides are extensively used in agriculture to safeguard crops; however, this widespread use poses serious health risks, especially for plastic greenhouse workers. This study investigates the genotoxicity of pesticide exposure in 32 non-smoking female greenhouse workers aged 20–40 in Mersin, Türkiye. Using the Buccal Micronucleus Assay (BM-ASSAY), researchers assessed genetic damage, cell proliferation, and cell death. The exposed group was compared to a control group of 32 non-smoking women of the same age not exposed to genotoxic agents. Results showed significantly higher frequencies of micronuclei (MN), karyolysis (KL), binucleated cells (BN), broken egg (BE), and karyorrhexis (KR) in the exposed group (p < 0.001 and p < 0.05). The study also calculated the repair index to evaluate epithelial tissue recovery capacity. These findings highlight the importance of biomonitoring as a tool for detecting early genetic damage. The study emphasizes the urgent need for improved safety measures and ongoing health monitoring to protect greenhouse workers from long-term pesticide-related risks.

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暴露于不同农药的塑料大棚工人口腔上皮细胞的遗传损伤评估：土耳其梅尔沁的细胞遗传学生物监测研究

农药在农业中广泛使用，以保护作物；然而，这种广泛使用造成了严重的健康风险，特别是对塑料温室工人。本研究调查了32名年龄在20-40岁的不吸烟温室女工农药暴露的遗传毒性。利用口腔微核测定（BM-ASSAY），研究人员评估了遗传损伤、细胞增殖和细胞死亡。将暴露组与32名未暴露于基因毒性物质的同龄不吸烟妇女组成的对照组进行比较。结果暴露组微核（MN）、核溶解（KL）、双核细胞（BN）、破卵（BE）和核裂（KR）的发生频率显著高于暴露组（p <； 0.001和p <； 0.05）。本研究还计算了修复指数来评估上皮组织的恢复能力。这些发现突出了生物监测作为早期基因损伤检测工具的重要性。该研究强调，迫切需要改进安全措施和持续的健康监测，以保护温室工人免受与农药有关的长期风险。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.