Emerging Microbes & Infections最新文献_第4页

Identification of serum tRNA-derived small RNAs biosignature for diagnosis of tuberculosis.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-02-06 DOI: 10.1080/22221751.2025.2459132

Zikun Huang, Qing Luo, Cuifen Xiong, Haiyan Zhu, Chao Yu, Jianqing Xu, Yiping Peng, Junming Li, Aiping Le

{"title":"Identification of serum tRNA-derived small RNAs biosignature for diagnosis of tuberculosis.","authors":"Zikun Huang, Qing Luo, Cuifen Xiong, Haiyan Zhu, Chao Yu, Jianqing Xu, Yiping Peng, Junming Li, Aiping Le","doi":"10.1080/22221751.2025.2459132","DOIUrl":"10.1080/22221751.2025.2459132","url":null,"abstract":"The tRNA-derived small RNAs (tsRNAs) are a new class of non coding RNAs, which are stable in body fluids and can be used as potential biomarkers for disease diagnosis. However, the exact value of tsRNAs in the diagnosis of tuberculosis (TB) is still unclear. The objective of the present study was to evaluate the performance of the serum tsRNAs biosignature to distinguish between active TB, healthy controls, latent TB infection, and other respiratory diseases. The differential expression profiles of tsRNAs in serum from active TB patients and healthy controls were analyzed by high-throughput sequencing. A total of 905 subjects were prospectively recruited for our study from three different cohorts. Levels of tsRNA-Gly-CCC-2, tsRNA-Gly-GCC-1, and tsRNA-Lys-CTT-2-M2 were significantly elevated in the serum of TB patients compared to non-TB individuals, showing a correlation with lung injury severity and acid-fast bacilli grades in TB patients. The accuracy of the three-tsRNA biosignature for TB diagnosis was evaluated in the training (n = 289), test (n = 124), and prediction (n = 292) groups. By utilizing cross-validation with a random forest algorithm approach, the training cohort achieved a sensitivity of 100% and specificity of 100%. The test cohort exhibited a sensitivity of 75.8% and a specificity of 91.2%. Within the prediction group, the sensitivity and specificity were 73.1% and 92.5%, respectively. The three-tsRNA biosignature generally decreased within 3 months of treatment and then remained stable. In conclusion, the three-tsRNA biosignature might serve as biomarker to diagnose TB and to monitor the effectiveness of treatment in a high-burden TB clinical setting.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2459132"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-neutralizing activity of the chikungunya vaccine VLA1553 against three prevalent chikungunya lineages.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/22221751.2025.2469653

Karin Kosulin, Trevor L Brasel, Jeanon Smith, Maricela Torres, Annegret Bitzer, Katrin Dubischar, Vera Buerger, Robert Mader, Scott C Weaver, David W C Beasley, Romana Hochreiter

{"title":"Cross-neutralizing activity of the chikungunya vaccine VLA1553 against three prevalent chikungunya lineages.","authors":"Karin Kosulin, Trevor L Brasel, Jeanon Smith, Maricela Torres, Annegret Bitzer, Katrin Dubischar, Vera Buerger, Robert Mader, Scott C Weaver, David W C Beasley, Romana Hochreiter","doi":"10.1080/22221751.2025.2469653","DOIUrl":"10.1080/22221751.2025.2469653","url":null,"abstract":"Cross-neutralization is generally a prerequisite for cross-protection of vaccines against diseases caused by heterologous viruses. Using sera obtained from a randomized clinical phase 3 trial in adults, we investigated the cross-neutralization activity of VLA1553, a vaccine recently approved to prevent chikungunya disease. Analysed in a plaque reduction neutralization test, the three major chikungunya virus (CHIKV) lineages, namely the East Central South African, the West African, and the Asian lineage, were inhibited by CHIKV-specific neutralizing antibodies present in the sera from vaccinated humans. This effect was independent of the time elapsed since vaccination. Moreover, the magnitude of the immune response was similar to the antibody levels detected in sera from convalescent chikungunya patients. Thus, VLA1553 has the potential to diminish the burden of chikungunya disease on a global scale.Trial registration: ClinicalTrials.gov identifier: NCT04546724.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2469653"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The seroprevalence of adenoviruses since 2000¹.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-03-17 DOI: 10.1080/22221751.2025.2475831

Lingling Hong, Jiashun Li, Weikai Zeng, Yuhua Li, Changfa Yu, Shutao Zhao, Ling Chen, Ying Feng

{"title":"The seroprevalence of adenoviruses since 20001.","authors":"Lingling Hong, Jiashun Li, Weikai Zeng, Yuhua Li, Changfa Yu, Shutao Zhao, Ling Chen, Ying Feng","doi":"10.1080/22221751.2025.2475831","DOIUrl":"10.1080/22221751.2025.2475831","url":null,"abstract":"Human adenoviruses (Ad) are increasingly used as vaccine vectors, especially after Ad5, Ad26, and ChAdY25 (ChAdOx1) were employed as vectors for SARS-CoV-2 vaccines. So far, more than 116 adenovirus genotypes have been identified, divided into 7 species (A-G). Most adenoviruses do not cause diseases or are mildly pathogenic, with only species B and E leading to acute respiratory infections or conjunctival inflammation and species F causing gastrointestinal infections. Previous studies have shown that the seroprevalence of neutralizing antibodies against adenoviruses can be limiting when applying adenoviral vectors. On the other hand, for highly pathogenic adenoviruses, neutralizing antibodies is beneficial for preventing the diseases caused by these adenoviruses. Here, we summarized the studies on the seroprevalence of adenoviruses, especially adenoviruses that may be utilized as vectors for vaccine and gene therapy. We also analysed possible factors associated with the seroprevalence and neutralizing titres. Given the trend of increasing adenoviral vector application, it is necessary to continue the investigation of the seroprevalence of neutralizing antibodies against adenoviruses in different geographic locations and populations.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2475831"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling distinct patterns of metagenomic surveillance and respiratory microbiota between two P1 genotypes of Mycoplasma pneumoniae. 揭示两种P1基因型肺炎支原体宏基因组监测和呼吸微生物群的不同模式。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-13 DOI: 10.1080/22221751.2024.2449087

Hailong You, Bin Yang, Huifang Liu, Wencai Wu, Fei Yu, Nan Lin, WenJiao Yang, Bingxue Hu, Yong Liu, Hongyan Zou, Sijia Hao, Yunping Xiao, Teng Xu, Yanfang Jiang

{"title":"Unravelling distinct patterns of metagenomic surveillance and respiratory microbiota between two P1 genotypes of Mycoplasma pneumoniae.","authors":"Hailong You, Bin Yang, Huifang Liu, Wencai Wu, Fei Yu, Nan Lin, WenJiao Yang, Bingxue Hu, Yong Liu, Hongyan Zou, Sijia Hao, Yunping Xiao, Teng Xu, Yanfang Jiang","doi":"10.1080/22221751.2024.2449087","DOIUrl":"10.1080/22221751.2024.2449087","url":null,"abstract":"To unravel distinct patterns of metagenomic surveillance and respiratory microbiota between Mycoplasma pneumoniae (M. pneumoniae) P1-1 and P1-2 and to explore the impact of the COVID-19 pandemic on epidemiological features, we conducted a multicentre retrospective study which spanned 90,886 pneumonia patients, among which 3164 cases M. pneumoniae were identified. Our findings revealed a concurrent outbreak of M. pneumoniae, with the positivity rate rising sharply to 9.62% from July 2023, compared to the 0.16% to 4.06% positivity rate observed during the 2020-2022 COVID-19 pandemic. P1-1 had a higher odds ratio of co-detecting opportunistic pathogens. However, no significant differences were observed in the co-detection odds ratio between children and other age groups in P1-2. This study is the first to demonstrate differences in relative abundance, diversity of respiratory microbiota and co-detection rate of opportunistic pathogen between M. pneumoniae P1-1 and P1-2. Through bronchoalveolar lavage (BAL) metagenomic and host transcriptomic analyses, we identified variations in co-detection rates of M. pneumoniae P1-1 genotype with opportunistic pathogens like S. pneumoniae, alterations in respiratory microbiota composition, lung inflammation, and disruption of ciliary function. Consistent with the results of host transcriptome, we found that P1-1 infections were associated with significantly higher rates of requiring respiratory support and mechanical ventilation compared to P1-2 infections (Fisher's exact test, p-value = 0.035/0.004). Our study provides preliminary evidence of clinical severity between M. pneumoniae strains, underscoring the need for ongoing research and development of targeted therapeutic strategies.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2449087"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly pathogenic avian H5N1 influenza A virus replication in ex vivo cultures of bovine mammary gland and teat tissues. 高致病性H5N1甲型禽流感病毒在牛乳腺和乳腺组织体外培养物中的复制。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-15 DOI: 10.1080/22221751.2025.2450029

Masaki Imai, Hiroshi Ueki, Mutsumi Ito, Kiyoko Iwatsuki-Horimoto, Maki Kiso, Asim Biswas, Sanja Trifkovic, Nigel Cook, Peter J Halfmann, Gabriele Neumann, Amie J Eisfeld, Yoshihiro Kawaoka

{"title":"Highly pathogenic avian H5N1 influenza A virus replication in ex vivo cultures of bovine mammary gland and teat tissues.","authors":"Masaki Imai, Hiroshi Ueki, Mutsumi Ito, Kiyoko Iwatsuki-Horimoto, Maki Kiso, Asim Biswas, Sanja Trifkovic, Nigel Cook, Peter J Halfmann, Gabriele Neumann, Amie J Eisfeld, Yoshihiro Kawaoka","doi":"10.1080/22221751.2025.2450029","DOIUrl":"10.1080/22221751.2025.2450029","url":null,"abstract":"Since early 2024, highly pathogenic avian influenza H5N1 viruses have been causing outbreaks in dairy cattle in the United States. Here, we compared the replicative capacity of A/dairy cattle/Texas/24-008749-001/2024 (H5N1; Cow-H5N1) isolated from a dairy cow, A/chicken/Ghana/AVL-76321VIR7050-39/2021 (H5N1; Chicken-H5N1) isolated from a chicken, and a human H1N1 2009 pandemic virus in ex vivo explant cultures of mammary gland and teat from lactating cows. We also examined the expression of influenza virus receptors in these organs. We observed that human influenza virus receptors are widely distributed throughout the epithelium of alveoli, ducts, and gland cisterns within the mammary gland, and in the teat cistern epithelium of dairy cattle, whereas avian influenza virus receptors are distributed on the alveolar, ductal, and teat cistern epithelium. We also found that Cow-H5N1 virus replicates more efficiently than Chicken-H5N1 or human H1N1pdm viruses in the gland cistern epithelium of dairy cattle. Notably, bovine H5N1 viruses replicated efficiently in the epithelium of the bovine teat cistern. These findings suggest that H5N1 viruses invade the mammary gland through the teat canal, which is easily accessed by viruses.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2450029"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nationwide seroprevalence of SARS-CoV-2 Delta variant and five Omicron sublineages in companion cats and dogs in the USA: insights into their role in COVID-19 epidemiology. 美国伴侣猫狗中SARS-CoV-2 δ变体和5个欧米克隆亚谱系的全国血清患病率：它们在COVID-19流行病学中的作用

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-11 DOI: 10.1080/22221751.2024.2437246

Subarna Barua, Nneka Vivian Iduu, Daniel Felipe Barrantes Murillo, Asfiha Tarannum, Hill Dimino, Suchita Barua, Yue Shu, Calvin Johnson, Megan R Miller, Kelly Chenoweth, Peter Christopherson, Laura Huber, Theresa Wood, Kelley Turner, Chengming Wang

{"title":"Nationwide seroprevalence of SARS-CoV-2 Delta variant and five Omicron sublineages in companion cats and dogs in the USA: insights into their role in COVID-19 epidemiology.","authors":"Subarna Barua, Nneka Vivian Iduu, Daniel Felipe Barrantes Murillo, Asfiha Tarannum, Hill Dimino, Suchita Barua, Yue Shu, Calvin Johnson, Megan R Miller, Kelly Chenoweth, Peter Christopherson, Laura Huber, Theresa Wood, Kelley Turner, Chengming Wang","doi":"10.1080/22221751.2024.2437246","DOIUrl":"10.1080/22221751.2024.2437246","url":null,"abstract":"Understanding SARS-CoV-2 epidemiology in companion animals is critical for evaluating their role in viral transmission and their potential as sentinels for human infections. This large-scale serosurvey analyzed serum samples from 706 cats and 2,396 dogs collected across the USA in 2023 using a surrogate virus neutralization test (sVNT) to detect SARS-CoV-2 antibodies. Overall, 5.7% of cats and 4.7% of dogs tested positive for antibodies, with younger animals (under 12 months) showing significantly lower seropositivity rates (p = 0.0048). Additionally, we analyzed 153 positive samples for variant-specific antibody responses using six sVNT kits targeting the Delta variant and five Omicron sublineages. Among cats, 67.5% showed antibodies to Delta, with positivity rates for Omicron sublineages as follows: BA.1 (62.5%), BA.2 (42.5%), BA.4/BA.5 (77.5%), XBB (52.5%), and XBB.1.5 (45.0%). In dogs, 55.8% were positive for Delta, and Omicron sublineage rates were BA.1 (46.0%), BA.4/BA.5 (37.2%), XBB (58.4%), BA.2 (13.3%), and XBB.1.5 (9.7%). Given the close contact between companion animals and humans, and the persistence of antibodies against various SARS-CoV-2 variants and sublineages, our findings suggest that seroprevalence in cats and dogs may serve as valuable tool for tracking COVID-19 epidemiology.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2437246"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unprecedented large outbreak of Plasmodium malariae malaria in Vietnam: Epidemiological and clinical perspectives. 越南史无前例的大规模疟疾疟原虫疫情：流行病学和临床视角。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-16 DOI: 10.1080/22221751.2024.2432359

Chau Van Khanh, Hương Giang Lê, Tuấn Cường Võ, Nguyen Xuan Quang, Do Van Nguyen, Nguyen Cong Trung Dung, Le Thanh Tam, Nguyen Thanh Thuy Nhien, Đăng Thùy Dương Nguyễn, Thu Hằng Nguyễn, Tran Thi Hue Van, Le Duc Vinh, Pham Minh Quan, Nguyen Kim Trung, Jung-Mi Kang, Byoung-Kuk Na, Huynh Hong Quang

引用次数: 0

Beyond the infection: mapping the risk of cardiovascular events post-scrub typhus in a nationwide cohort study.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-02-20 DOI: 10.1080/22221751.2025.2467766

Jih-Kai Yeh, Victor Chien-Chia Wu, Shao-Wei Chen, Chia-Ling Wu, Yu-Sheng Lin, Chun-Wen Cheng, Chih-Hsiang Chang, Michael Wu, Pao-Hsien Chu, Shang-Hung Chang, Yu-Tung Huang

{"title":"Beyond the infection: mapping the risk of cardiovascular events post-scrub typhus in a nationwide cohort study.","authors":"Jih-Kai Yeh, Victor Chien-Chia Wu, Shao-Wei Chen, Chia-Ling Wu, Yu-Sheng Lin, Chun-Wen Cheng, Chih-Hsiang Chang, Michael Wu, Pao-Hsien Chu, Shang-Hung Chang, Yu-Tung Huang","doi":"10.1080/22221751.2025.2467766","DOIUrl":"10.1080/22221751.2025.2467766","url":null,"abstract":"ABSTRACTScrub typhus, caused by Orientia tsutsugamushi, often involves multiple organs, but its cardiovascular (CV) sequelae in survivors remain under-researched. This retrospective cohort study analyzed data from the National Health Insurance Research Database (NHIRD) spanning 2010-2015 to assess CV risks among scrub typhus survivors. Excluding those with prior CV events, we focused on outcomes such as acute myocardial infarction (AMI), heart failure hospitalization (HFH), strokes, new-onset atrial fibrillation (AF), aortic aneurysm or dissection, venous thromboembolism (VTE), and CV death. From 2,269 scrub typhus patients without previous CV events (mean age 47.8 ± 16.1; 38.0% female), and a matched control group (n = 2,264), we observed a higher incidence of HFH, new-onset AF, and total CV events in the scrub typhus cohort. Adjusted hazard ratios (aHRs) were 1.97 (95% CI: 1.13-3.42) for HFH, 2.48 (95% CI: 1.23-5.0) for new-onset AF, and 1.43 (95% CI: 1.08-1.91) for total CV events. Other outcomes did not significantly differ. Scrub typhus survivors exhibit an increased risk of CV events, particularly HFH and new-onset AF, underscoring the importance of heightened physician awareness and post-infection cardiac surveillance.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2467766"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SREBP2-dependent lipid droplet formation enhances viral replication and deteriorates lung injury in mice following IAV infection.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-02-28 DOI: 10.1080/22221751.2025.2470371

Xinsen Li, Lu Li, Jijing Tian, Ruijing Su, Jiali Sun, Yuli Li, Lige Wang, Hongye Zhou, Shuhan Sha, Jin Xiao, Hong Dong, Caiyun Huo, Yanxin Hu, Hanchun Yang

{"title":"SREBP2-dependent lipid droplet formation enhances viral replication and deteriorates lung injury in mice following IAV infection.","authors":"Xinsen Li, Lu Li, Jijing Tian, Ruijing Su, Jiali Sun, Yuli Li, Lige Wang, Hongye Zhou, Shuhan Sha, Jin Xiao, Hong Dong, Caiyun Huo, Yanxin Hu, Hanchun Yang","doi":"10.1080/22221751.2025.2470371","DOIUrl":"10.1080/22221751.2025.2470371","url":null,"abstract":"Influenza A virus (IAV) is a significant zoonotic pathogen that poses a considerable challenge to public health due to its continuous mutations. Lipid droplets (LDs) have been shown to play an important role in the process of several viral infections. However, their role in IAV infection remains unclear. Here, we found that IAV infection altered the lipid metabolism and increased the content of LDs in the lungs of mice. In vitro, IAV infection also mediated the formation of LDs in A549 cells. Besides, inhibition of the formation of lipid droplets can significantly suppress IAV replication and the release of inflammatory factors, indicating that LDs could facilitate the virus replication and inflammatory response. Furthermore, we discovered that IAV infection could activate the SREBP2, a crucial lipid-regulating transcription factor that regulates the expressions of downstream proteins named HMGCR and HMGCS. HMGCR and HMGCS involved in the process of cholesterol synthesis, which further promoted the formation of LDs. Additionally, the use of fatostatin that specifically inhibits the maturation of SREBP2 was able to significantly suppress the viral replication of H5N1 in cells and effectively ameliorated IAV-induced lung injury in mice, which eventually promoted the survival rate of infected mice. Taken together, we demonstrate the essential roles of lipid metabolism and LD formation in IAV replication and pathogenesis, which may better facilitate the advancement of new strategies against IAV infection, especially the highly pathogenic H5N1 virus.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2470371"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paediatric varicella epidemiology in urban Beijing, China, 2014-2023.

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-02-28 DOI: 10.1080/22221751.2025.2467773

Dan Yu, Wei Su, Miao Fan, Lu Lu, Xinyu Wang, Sheng Zhang, Qingjing Du, Lin Ma, Ying Liu, Kaihu Yao

引用次数: 0