Emerging Microbes & Infections最新文献

Isoniazid potentiates tigecycline to kill methicillin-resistant Staphylococcus aureus. 异烟肼能增强替加环素杀死耐甲氧西林金黄色葡萄球菌的能力。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-09 DOI: 10.1080/22221751.2024.2434587

Xuan-Wei Chen, Hao-Qing Chen, Jia-Han Wu, Zhi-Han Wang, Yu-Qing Zhou, Si-Qi Tian, Bo Peng

{"title":"Isoniazid potentiates tigecycline to kill methicillin-resistant Staphylococcus aureus.","authors":"Xuan-Wei Chen, Hao-Qing Chen, Jia-Han Wu, Zhi-Han Wang, Yu-Qing Zhou, Si-Qi Tian, Bo Peng","doi":"10.1080/22221751.2024.2434587","DOIUrl":"10.1080/22221751.2024.2434587","url":null,"abstract":"Therapeutic option for treating methicillin-resistant Staphylococcus aureus (MRSA) infection is urgently required since its resistance to a broad spectrum of currently available antibiotics. Here, we report that isoniazid is able to potentiate the killing efficacy of tigecycline to MRSA. The combination of isoniazid and tigecycline reduces the minimal inhibitory concentration of clinic MRSA strains to tigecycline. The killing activity of tigecycline is further confirmed by killing experiments and murine infection model. We further demonstrate the mechanism that isoniazid increases intracellular accumulation of tigecycline by promoting the influx but limiting the efflux of tigecycline through proton motive force. We also show that isoniazid and tigecycline synergize to increase the abundance of isoniazid-NAD adduct, which in turn damage cell membrane, possibly contributing to the disruption of PMF. Whereas phosphatidylethanolamine and cardiolipin are able to abrogate the synergistic effect of isoniazid plus tigecycline. Thus our study provides a new perspective that antibiotics, e.g. isoniazid, once recognized only to target Mycobacterium tuberculosis, can be repurposed as antibiotic adjuvant to tigecycline, expanding our choice of antibiotic-antibiotic combinations in treating bacterial infectious diseases.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2434587"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterizations of Klebsiella variicola: emerging pathogens identified from sepsis patients in Ethiopian referral hospitals. 变异克雷伯氏菌的基因组特征：从埃塞俄比亚转诊医院败血症患者中发现的新病原体。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-18 DOI: 10.1080/22221751.2024.2440494

Melese Hailu Legese, Daniel Asrat, Adane Mihret, Badrul Hasan, Abraham Aseffa, Göte Swedberg

{"title":"Genomic characterizations of Klebsiella variicola: emerging pathogens identified from sepsis patients in Ethiopian referral hospitals.","authors":"Melese Hailu Legese, Daniel Asrat, Adane Mihret, Badrul Hasan, Abraham Aseffa, Göte Swedberg","doi":"10.1080/22221751.2024.2440494","DOIUrl":"10.1080/22221751.2024.2440494","url":null,"abstract":"Healthcare in low- and middle-income countries is becoming problematic due to the emergence of multidrug-resistant bacteria causing serious morbidity and mortality. Klebsiella variicola carrying multiple antimicrobial resistance (AMR) genes were found significantly among sepsis patients in a study done between October 2019 and September 2020 at four Ethiopian hospitals located in the central (Tikur Anbessa and Yekatit 12), southern (Hawassa), and northern (Dessie) parts. Among 1416 sepsis patients, 74 K. variicola isolates were identified using MALDI-TOF, most of them at Dessie (n = 44) and Hawassa (n = 28) hospitals. Whole genome sequencing showed that K. variicola strains identified at Dessie Hospital displayed phylogenetic clonality, carried an IncM1 plasmid and the majority were ST3924. Many K. variicola identified at Hawassa Hospital were clonally clustered and the majority belonged to novel STs and carried IncFIB(K) and IncFII(K) plasmids concurrently. Fifty K. variicola carried ESBL genes while 2 isolates harboured AmpC. Other frequently found genes were aac(3)-lla, blaCTX-M-15, blaTEM-1B, blaLEN2, blaOXA-1, blaSCO-1, catB3, dfrA14, QnrB1, aac(6')-lb-cr and sul2. Virulence genes detected at both sites were mrk operons for biofilm formation and siderophore ABC transporter operons for iron uptake. Capsular alleles varied, with wzi 269 at Dessie and wzi 582 at Hawassa. The isolation of multidrug-resistant K. variicola as an emerging sepsis pathogen calls for strong infection prevention strategies and antimicrobial stewardship supported by advanced bacterial identification techniques.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2440494"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The westward spread dynamics of cholera from the eastern endemic Democratic Republic of the Congo. 霍乱从东部流行的刚果民主共和国向西传播的动态。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-09 DOI: 10.1080/22221751.2024.2437245

Harry César Ntumba Kayembe, Nadège Makuntima Taty, Hippolyte Nani-Tuma Situakibanza

引用次数: 0

A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease. 再现人类马尔堡病毒病关键方面的符合bsl2的替代感染模型

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-12 DOI: 10.1080/22221751.2024.2449083

Wanying Yang, Wujie Zhou, Bo Liang, Xiaojun Hu, Shen Wang, Zhenshan Wang, Tiecheng Wang, Xianzhu Xia, Na Feng, Yongkun Zhao, Feihu Yan

{"title":"A surrogate BSL2-compliant infection model recapitulating key aspects of human Marburg virus disease.","authors":"Wanying Yang, Wujie Zhou, Bo Liang, Xiaojun Hu, Shen Wang, Zhenshan Wang, Tiecheng Wang, Xianzhu Xia, Na Feng, Yongkun Zhao, Feihu Yan","doi":"10.1080/22221751.2024.2449083","DOIUrl":"10.1080/22221751.2024.2449083","url":null,"abstract":"Marburg virus disease (MVD) is a severe infectious disease caused by the Marburg virus (MARV), posing a significant threat to humans. MARV needs to be operated under strict biosafety Level 4 (BSL-4) laboratory conditions. Therefore, accessible and practical animal models are urgently needed to advance prophylactic and therapeutic strategies for MARV. In this study, we constructed a recombinant vesicular stomatitis virus (VSV) expressing the Marburg virus glycoprotein (VSV-MARV/GP). Syrian hamsters infected with VSV-MARV/GP presented symptoms such as thrombocytopenia, lymphopenia, haemophilia, and multiorgan failure, developing a severe systemic disease akin to that observed in human MARV patients. Notably, the pathogenicity was found to be species-specific, age-related, sex-associated, and challenge route-dependent. Subsequently, the therapeutic efficacy of the MR191 monoclonal antibody was validated in this model. In summary, this alternative model is an effective tool for rapidly screening medical countermeasures against MARV GP in vivo under BSL-2 conditions.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2449083"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance in hypermucoviscous and non-hypermucoviscous Klebsiella pneumoniae: a systematic review and meta-analysis. 高黏性和非高黏性肺炎克雷伯菌的抗菌素耐药性：一项系统综述和荟萃分析。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-16 DOI: 10.1080/22221751.2024.2438657

Hiroki Namikawa, Ken-Ichi Oinuma, Yukihiro Kaneko, Hiroshi Kakeya, Taichi Shuto

{"title":"Antimicrobial resistance in hypermucoviscous and non-hypermucoviscous Klebsiella pneumoniae: a systematic review and meta-analysis.","authors":"Hiroki Namikawa, Ken-Ichi Oinuma, Yukihiro Kaneko, Hiroshi Kakeya, Taichi Shuto","doi":"10.1080/22221751.2024.2438657","DOIUrl":"10.1080/22221751.2024.2438657","url":null,"abstract":"Antimicrobial resistance has recently increased due to emerging carbapenem-resistant Klebsiella pneumoniae and extended-spectrum β-lactamase (ESBL)-producing strains of K. pneumoniae, especially among hypermucoviscous K. pneumoniae (hmKp) strains. To evaluate the prevalence of ESBL-producing and carbapenem-resistant strains in hmKp and non-hmKp clinical isolates through a systematic review and meta-analysis. We searched PubMed, Scopus, and Cochrane Library databases from January 2000 to June 2023. Clinical and in vivo/in vitro studies involving confirmed K. pneumoniae clinical isolates differentiated into hmKP and non-hmKP strains based on string test results. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated based on the number of individuals in each target group. Forest plots were used to visualize the effect sizes and 95% CIs of individual studies estimated using the inverse variance and DerSimonian - Laird methods with fixed - and random-effects models, respectively. Heterogeneity was assessed using Cochran's Q test (I2 ≥ 50%). Fifteen studies comprising 2049 clinical isolates of K. pneumoniae met the inclusion criteria. Meta-analysis revealed that hmKp strains were associated with a significantly lower prevalence of ESBL-producing strains (pooled OR: 0.26, 95% CI: 0.11-0.63, P = 0.003) and a slightly lower prevalence of carbapenem-resistant strains than non-hmKp strains (pooled OR: 0.63, 95% CI: 0.40-0.97, P = 0.038). hmKp strains exhibited lower and slightly lower prevalence of ESBL production and carbapenem resistance, respectively, than non-hmKp strains. However, given the rising prevalence of ESBL-producing and carbapenem-resistant hmKp strains, patients infected by string-test-positive K. pneumoniae must be managed prudently, considering the potential for highly resistant strains.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2438657"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term impact of molecular epidemiology shifts of methicillin-resistant Staphylococcus aureus on severity and mortality of bloodstream infection. 耐甲氧西林金黄色葡萄球菌分子流行病学变化对血流感染严重程度和死亡率的长期影响

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-09 DOI: 10.1080/22221751.2024.2449085

Norihito Kaku, Masaki Ishige, Go Yasutake, Daisuke Sasaki, Kenji Ota, Fujiko Mitsumoto-Kaseida, Kosuke Kosai, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

{"title":"Long-term impact of molecular epidemiology shifts of methicillin-resistant Staphylococcus aureus on severity and mortality of bloodstream infection.","authors":"Norihito Kaku, Masaki Ishige, Go Yasutake, Daisuke Sasaki, Kenji Ota, Fujiko Mitsumoto-Kaseida, Kosuke Kosai, Hiroo Hasegawa, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara","doi":"10.1080/22221751.2024.2449085","DOIUrl":"10.1080/22221751.2024.2449085","url":null,"abstract":"A 2019 nationwide study in Japan revealed the predominant methicillin-resistant Staphylococcus aureus (MRSA) types in bloodstream infections (BSIs) to be sequence type (ST)8-carrying SCCmec type IV (ST8-MRSA-IV) and clonal complex 1-carrying SCCmec type IV (CC1-MRSA-IV). However, detailed patient characteristics and how these MRSA types evolve over time remain largely unknown. In this long-term single-center study, MRSA strains isolated from blood cultures at Nagasaki University Hospital from 2012 to 2019 were sequenced and analyzed. Additionally, we compared the SCCmec types and patient characteristics identified in this study with previous data from our hospital spanning 2003-2007 and 2008-2011. Over this 16-year period, SCCmec type II decreased significantly from 79.2% to 15.5%, while type IV increased from 18.2% to 65.5%. This shift in SCCmec types was associated with notable changes in severity and outcomes; the sequential organ failure assessment (SOFA) score decreased from 5.8 to 3.1; in-hospital mortality declined from 39.8% to 15.5%. In contrast, no significant changes in patient demographics, such as age, sex, or underlying diseases, were observed. Between 2012 and 2019, the major combinations of SCCmec type and sequence type were ST8-MRSA-IV, ST8-MRSA-I, CC1-MRSA-IV, and ST5-MRSA-II. Additionally, ST8-MRSA-IV was divided into CA-MRSA/J, t5071-ST8-MRSA-IV, and USA300-like clone based on the results of molecular analysis. These major combinations showed similar drug resistance patterns, molecular characteristics, and phylogenetic features to those identified in nationwide surveillance. This study highlights the evolving nature of MRSA types in bloodstream infections, correlating with improved patient outcomes over time.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"14 1","pages":"2449085"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing wastewater surveillance to model behavioural responses and prevent healthcare overload during "Disease X" outbreaks. 利用废水监测模拟行为反应，防止“疾病X”爆发期间医疗保健超载。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-18 DOI: 10.1080/22221751.2024.2437240

Wenxiu Chen, Wei An, Chen Wang, Qun Gao, Chunzhen Wang, Lan Zhang, Xiao Zhang, Song Tang, Jianxin Zhang, Lixin Yu, Peng Wang, Dan Gao, Zhe Wang, Wenhui Gao, Zhe Tian, Yu Zhang, Wai-Yin Ng, Tong Zhang, Ho-Kwong Chui, Jianying Hu, Min Yang

{"title":"Utilizing wastewater surveillance to model behavioural responses and prevent healthcare overload during \"Disease X\" outbreaks.","authors":"Wenxiu Chen, Wei An, Chen Wang, Qun Gao, Chunzhen Wang, Lan Zhang, Xiao Zhang, Song Tang, Jianxin Zhang, Lixin Yu, Peng Wang, Dan Gao, Zhe Wang, Wenhui Gao, Zhe Tian, Yu Zhang, Wai-Yin Ng, Tong Zhang, Ho-Kwong Chui, Jianying Hu, Min Yang","doi":"10.1080/22221751.2024.2437240","DOIUrl":"10.1080/22221751.2024.2437240","url":null,"abstract":"During the COVID-19 pandemic, healthcare systems worldwide faced severe strain. This study, utilizing wastewater virus surveillance, identified that periodic spontaneous avoidance behaviours significantly impacted infectious disease transmission during rapid and intense outbreaks. To incorporate these behaviours into disease transmission analysis, we introduced the Su-SEIQR model and validated it using COVID-19 wastewater data from Beijing and Hong Kong. The results demonstrated that the Su-SEIQR model accurately reflected trends in susceptible populations and confirmed cases during the COVID-19 pandemic, highlighting the role of spontaneous collective avoidance behaviours in generating periodic fluctuations. These fluctuations helped reduce infection peaks, thereby alleviating pressure on healthcare systems. However, the effect of these spontaneous behaviours on mitigating healthcare overload was limited. Consequently, we incorporated healthcare capacity constraints into the model, adjusting parameters to further guide population behaviours during the pandemic, aiming to keep the outbreak within manageable limits and reduce strain on healthcare resources. This study provides robust support for the development of environmental and public health policies during pandemics by constructing an innovative transmission model, which effectively prevents healthcare overload. Additionally, this approach can be applied to managing future outbreaks of unknown viruses or \"Disease X\".","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2437240"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic cats. H5N1进化分支2.3.4.4的显着嗜神经性和潜在适应性。b病毒在自然感染的家猫。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-17 DOI: 10.1080/22221751.2024.2440498

Shubhada K Chothe, Surabhi Srinivas, Sougat Misra, Noel Chandan Nallipogu, Elizabeth Gilbride, Lindsey LaBella, Swastidipa Mukherjee, Christian H Gauthier, Heidi L Pecoraro, Brett T Webb, James M Pipas, Santhamani Ramasamy, Suresh V Kuchipudi

{"title":"Marked neurotropism and potential adaptation of H5N1 clade 2.3.4.4.b virus in naturally infected domestic cats.","authors":"Shubhada K Chothe, Surabhi Srinivas, Sougat Misra, Noel Chandan Nallipogu, Elizabeth Gilbride, Lindsey LaBella, Swastidipa Mukherjee, Christian H Gauthier, Heidi L Pecoraro, Brett T Webb, James M Pipas, Santhamani Ramasamy, Suresh V Kuchipudi","doi":"10.1080/22221751.2024.2440498","DOIUrl":"10.1080/22221751.2024.2440498","url":null,"abstract":"In April 2024, ten cats died in a rural South Dakota (SD) residence, showing respiratory and neurological symptoms. Necropsy and laboratory testing of two cats confirmed H5N1 clade 2.3.4.4b infection. The viral genome sequences are closely related to recent SD cattle H5N1 sequences. Cat H5N1 genomes had unique mutations, including T143A in haemagglutinin, known to affect infectivity and immune evasion, and two novel mutations in PA protein (F314L, L342Q) that may affect polymerase activity and virulence, suggesting potential virus adaptation. Dead cats showed systemic infection with lesions and viral antigens in multiple organs. Higher viral RNA and antigen in the brain indicated pronounced neurotropism. Lectin-histochemistry revealed widespread co-expression of sialic acid α-2,6 and α-2,3 receptors, suggesting cats could serve as mixing vessels for reassortment of avian and mammalian influenza viruses. No differences in clade 2.2 or 2.3.4.4b H5 pseudoviruses binding to cat lung/brain tissues indicated the neurotropism is unlikely mediated by receptor binding affinity.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2440498"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV. 来自非流行地区的蚊子种群具有传播新近出现的日本脑炎病毒基因型IV的潜力。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-01-02 DOI: 10.1080/22221751.2024.2438661

Astri Nur Faizah, Daisuke Kobayashi, Faustus Akankperiwen Azerigyik, Ryo Matsumura, Izumi Kai, Yoshihide Maekawa, Yukiko Higa, Kentaro Itokawa, Toshinori Sasaki, Kris Cahyo Mulyatno, Sri Subekti, Maria Inge Lusida, Etik Ainun Rohmah, Yasuko Mori, Yusuf Ozbel, Chizu Sanjoba, Tran Vu Phong, Tran Cong Tu, Shinji Kasai, Kyoko Sawabe, Haruhiko Isawa

{"title":"Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV.","authors":"Astri Nur Faizah, Daisuke Kobayashi, Faustus Akankperiwen Azerigyik, Ryo Matsumura, Izumi Kai, Yoshihide Maekawa, Yukiko Higa, Kentaro Itokawa, Toshinori Sasaki, Kris Cahyo Mulyatno, Sri Subekti, Maria Inge Lusida, Etik Ainun Rohmah, Yasuko Mori, Yusuf Ozbel, Chizu Sanjoba, Tran Vu Phong, Tran Cong Tu, Shinji Kasai, Kyoko Sawabe, Haruhiko Isawa","doi":"10.1080/22221751.2024.2438661","DOIUrl":"10.1080/22221751.2024.2438661","url":null,"abstract":"Japanese encephalitis virus (JEV) genotype IV (GIV) is one of the least common and most neglected genotypes worldwide, having been identified only on a few Indonesian islands until it was recently found to be the cause of outbreaks that occurred in several Australian states in early 2022. Given the limited availability of information, the vector range for JEV GIV remains unknown; thus, understanding this range could prove invaluable for future prevention efforts in new areas. Herein, we experimentally exposed four mosquito colonies originated from various countries with no previous reports of GIV to JEV GIV strain 19CxBa-83-Cv, which was isolated from Culex vishnui Theobald collected in Bali in 2019. At 7 and 14 days post-JEV GIV exposure through a membrane feeding method, mosquito bodies, head-wings-legs, and saliva were harvested for infection, dissemination, and transmission efficiency analyses. The results showed robust transmission efficiencies of the virus by Culex tritaeniorhynchus Giles (∼74%) and Aedes albopictus Skuse (∼52%) from Japan, followed by Culex quinquefasciatus Say from Vietnam (∼35%) and Culex pipiens form molestus from Turkey (∼18%). Although significant differences were observed, we found that the four mosquito species could transmit JEV GIV. The efficiency of biological transmission of this restricted genotype by mosquitoes from various origins suggests that these mosquito species could support localized transmission if the genotype were introduced to their respective areas. This study emphasizes the importance of remaining vigilant and continuing arbovirus surveillance in all locations.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2438661"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automatic identification of clinically important Aspergillus species by artificial intelligence-based image recognition: proof-of-concept study. 基于人工智能的图像识别自动识别临床上重要的曲霉菌种：概念验证研究。

IF 8.4 2区医学

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2024-12-09 DOI: 10.1080/22221751.2024.2434573

Chi-Ching Tsang, Chenyang Zhao, Yueh Liu, Ken P K Lin, James Y M Tang, Kar-On Cheng, Franklin W N Chow, Weiming Yao, Ka-Fai Chan, Sharon N L Poon, Kelly Y C Wong, Lianyi Zhou, Oscar T N Mak, Jeremy C Y Lee, Suhui Zhao, Antonio H Y Ngan, Alan K L Wu, Kitty S C Fung, Tak-Lun Que, Jade L L Teng, Dirk Schnieders, Siu-Ming Yiu, Susanna K P Lau, Patrick C Y Woo

{"title":"Automatic identification of clinically important Aspergillus species by artificial intelligence-based image recognition: proof-of-concept study.","authors":"Chi-Ching Tsang, Chenyang Zhao, Yueh Liu, Ken P K Lin, James Y M Tang, Kar-On Cheng, Franklin W N Chow, Weiming Yao, Ka-Fai Chan, Sharon N L Poon, Kelly Y C Wong, Lianyi Zhou, Oscar T N Mak, Jeremy C Y Lee, Suhui Zhao, Antonio H Y Ngan, Alan K L Wu, Kitty S C Fung, Tak-Lun Que, Jade L L Teng, Dirk Schnieders, Siu-Ming Yiu, Susanna K P Lau, Patrick C Y Woo","doi":"10.1080/22221751.2024.2434573","DOIUrl":"10.1080/22221751.2024.2434573","url":null,"abstract":"While morphological examination is the most widely used for Aspergillus identification in clinical laboratories, PCR-sequencing and MALDI-TOF MS are emerging technologies in more financially-competent laboratories. However, mycological expertise, molecular biologists and/or expensive equipment are needed for these. Recently, artificial intelligence (AI), especially image recognition, is being increasingly employed in medicine for fast and automated disease diagnosis. We explored the potential utility of AI in identifying Aspergillus species. In this proof-of-concept study, using 2813, 2814 and 1240 images from four clinically important Aspergillus species for training, validation and testing, respectively; the performances and accuracies of automatic Aspergillus identification using colonial images by three different convolutional neural networks were evaluated. Results demonstrated that ResNet-18 outperformed Inception-v3 and DenseNet-121 and is the best algorithm of choice because it made the fewest misidentifications (n = 8) and possessed the highest testing accuracy (99.35%). Images showing more unique morphological features were more accurately identified. AI-based image recognition using colonial images is a promising technology for Aspergillus identification. Given its short turn-around-time, minimal demand of expertise, low reagent/equipment costs and user-friendliness, it has the potential to serve as a routine laboratory diagnostic tool after the database is further expanded.","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2434573"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0