Emerging Microbes & Infections最新文献

{"title":"HLA profiles and susceptibility to Alveolar echinococcosis: evidence from a large cohort with population and household controls.","authors":"Lynn Peters, Zuotang Ye, Alexander Grunenberg, Giuseppe Zenatti, Wanjie Jiang, Yasemin Özkan, Nele Wellinghausen, Teresa Rangel-Vivar, Benedikt Haggenmüller, Andreas Binzberger, Wolfgang Kratzer, Marko Kornmann, Erik Rasbach, Daniel Fürst, Beate Grüner","doi":"10.1080/22221751.2026.2744492","DOIUrl":"10.1080/22221751.2026.2744492","url":null,"abstract":"<p><p>Alveolar echinococcosis (AE) is an emerging and potentially fatal helminthic disease. Despite widespread environmental contamination with infective <i>Echinococcus multilocularis</i> (EM) eggs in endemic areas, human AE remains rare, suggesting an important role of host genetic susceptibility. In this case-control study, HLA variation was assessed by amplicon-based next-generation sequencing in 360 patients with AE and compared with (i) 1,440 population controls matched, where feasible, on age, sex, and place of residence, and (ii) 100 unrelated household controls to account for shared environmental exposure to EM. Analyses included age- and sex-adjusted Firth-penalized logistic regression, linkage disequilibrium (LD)-aware haplotype analyses, simulation-based maximum-score tests, and Benjamini-Hochberg false discovery rate correction. At two-field resolution, C*07:01 was associated with increased AE odds (OR 2.10; adjusted <i>p</i> = 0.006), whereas B*18:01 was associated with reduced odds (OR 0.49; adjusted <i>p</i> = 0.014). Haplotype analyses identified an extended LD-defined background involving A*29, C*16, or both, frequently combined with B*44, DRB1*07, or DQB1*02. A*29 carriage corresponded to the A01-A24 supertype category. A*29-DQB1*02 remained significant after within-model and study-wide correction (OR 3.92; adjusted <i>p</i> = 0.005 and 0.039, respectively), while C*16-B*44-DQB1*02 remained significant after within-model correction (OR 2.83; adjusted <i>p</i> = 0.041) but not after study-wide correction. Exploratory analyses of early-onset AE (≤35 years; n = 63) suggested associations with A*31:01 (OR 2.86) and DQB1*05:03 (OR 3.44), although neither remained significant after multiple-testing correction. These findings support a contribution of individual HLA alleles and extended haplotypic backgrounds to AE susceptibility within a multifactorial host-environment model.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2744492"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel hybrid alphavirus-Nipah virus pseudovirion for rapid quantification of vaccine-induced neutralization antibodies.","authors":"Brian Hetrick, Sravya Sowdamini Nakka, Dongyang Yu, Sarah Swineford, Nadia Storm, Anthony Griffiths, Yuntao Wu","doi":"10.1080/22221751.2026.2660425","DOIUrl":"10.1080/22221751.2026.2660425","url":null,"abstract":"<p><p>Nipah virus (NiV) is an emergent paramyxovirus that causes serious disease in humans and animals. Accurate quantification of neutralizing antibodies (nAB) against NiV is essential for vaccine development. The current standard, the plaque reduction neutralization test (PRNT), requires authentic infectious NiV and BSL-4 containment, taking 4-7 days to complete. In this study, we developed a hybrid alphavirus-Nipah virus pseudovirion (Ha-NiV) composed of a non-replicating Nipah virus virus-like particle (VLP) that encapsulates an RNA genome from a fast-expressing SFV (Semliki Forest Virus) alphaviral vector. Ha-NiV can infect NiV target cells but is replication incompetent, enabling rapid quantification of nAB (6-18 h) in BSL-2 conditions. We demonstrated concentration-dependent neutralization of Ha-NiV using an anti-NiV F glycoprotein antibody, 12B2, which is induced with a prefusion stabilized NiV F ectodomain trimer. We further validated the Ha-NiV-based neutralization assay using sera from Nipah virus-infected African green monkeys, and established a good correlation (<i>R</i>² = 0.86) with PRNT, demonstrating comparable specificity and sensitivity. These results demonstrate that the Ha-NiV assay can serve as a novel platform for convenient and rapid quantification of vaccine-induced nAB in BSL-2 settings.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2660425"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical immunogenicity assessment of HIV/AIDS vaccines: current progress and future perspectives.","authors":"Hang Yu, Xi Wu, Tingting Li, Jianhui Nie","doi":"10.1080/22221751.2026.2751675","DOIUrl":"10.1080/22221751.2026.2751675","url":null,"abstract":"<p><p>Developing a safe and effective HIV-1 vaccine remains the cornerstone of global efforts to terminate the AIDS pandemic. However, the virus's immense genetic diversity and the persistent absence of a universal Correlate of Protection (CoP) present formidable barriers. Concomitant with the iterative evolution of vaccine design - spanning from protein subunits to mRNA and germline-targeting platforms - immunogenicity evaluation frameworks have undergone a critical paradigm shift. The field has transitioned from relying on conventional serological assays to embracing comprehensive, high-dimensional immune profiling. This review delineates the trajectory of milestone clinical trials, including RV144, the HVTN series, and the AMP studies, to systematically summarize advancements in assessing both humoral and cellular immunity. We comprehensively examine core methodologies, such as Binding Antibody Multiplex Assays (BAMA), pseudovirus neutralization, Enzyme-Linked Immunospot (ELISpot), and Intracellular Cytokine Staining (ICS). Furthermore, we highlight a pivotal transition in evaluative logic: moving beyond a singular focus on neutralizing antibody titres to integrate Fc-mediated effector functions (e.g. Antibody-Dependent Cellular Cytotoxicity (ADCC) and Phagocytosis (ADCP)) and the synergistic roles of polyfunctional T-cells. We also explore the transformative potential of burgeoning frontiers, including systems serology, single-cell B-cell receptor (BCR) sequencing, and mucosal immunity evaluation. Ultimately, we recommend that future evaluation frameworks combine single-cell multi-omics technologies with artificial intelligence algorithms and integrate multi-dimensional data for analysis, thereby providing a solid theoretical and technical foundation for the design of next-generation HIV vaccines.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2751675"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of the novel PA-D27G mutation conferring reduced baloxavir susceptibility in influenza A viruses circulating in China, 2018-2025.","authors":"Binhuang Sun, Yulei Sun, Hongyu Wang, Xiaolu Tang, Wanggang Tang, Yabo Mi, Zhouying Shen, Quanlin Xue, Yinying Lu, Xiaoyu Zhao, Jingwen Ai, Jian Lu, Wenhong Zhang","doi":"10.1080/22221751.2026.2620222","DOIUrl":"10.1080/22221751.2026.2620222","url":null,"abstract":"<p><p>Seasonal influenza A viruses evolve rapidly, yet the emergence and molecular basis of resistance to the polymerase acidic (PA) inhibitor baloxavir marboxil (BXM), which is widely used in China, remain elusive. To address this, 3938 PA gene sequences were collected from influenza patients across mainland China between 2018 and 2025, from the national surveillance network and GISAID. By screening post-market mutations in the N-terminal domain of PA (PAN) that appeared in at least two samples and at a frequency below 50%, twenty-five single-point mutations were identified and additionally six linked mutations potentially associated with drug pressure. The impact of these mutations on BXM sensitivity was subsequently evaluated. Our analysis revealed the emergence of known mutations associated with reduced BXM sensitivity, including L28P, K34R, E198 K, although their prevalence remained low (2/3850, 0.05%). Notably, we identified a novel substitution, D27G, which conferred an approximately 12.4-fold reduction in BXM susceptibility compared with the wild-type virus and exhibited higher replication fitness than the canonical resistance mutation I38T, as demonstrated in human airway organoids. Molecular dynamics simulations further indicated that PA-D27G attenuates the interaction between PA and baloxavir acid, the active form of BXM. Epidemiological analysis showed that D27G mutation remained rare, being detected in four isolates (4/1247, 0.32%) in mainland China, and at a sporadic prevalence (<0.1%, 9/53132) across global isolates. In conclusion, these results demonstrate the early emergence of BXM-associated resistance in China and identify PA-D27G as a resistance-associated mutation with preserved viral fitness, underscoring the importance of continued genomic and epidemiologic surveillance.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2620222"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12865856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/22221751.2026.2712857","DOIUrl":"10.1080/22221751.2026.2712857","url":null,"abstract":"","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2712857"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148677482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Replication and compartmentalization of Dobrava-Belgrade virus in the central nervous system.","authors":"Julia Melchert, Julia Herzig-Nichtweiss, Jenny Meinhardt, Farid Salih, Till Dominik Best, Marie Luisa Schmidt, Lisa Jasmin Müller, Talitha Veith, Jörn Beheim-Schwarzbach, Jörg Hofmann, Terry C Jones, Eberhard Siebert, Felix Schoenrath, Helena Radbruch, Christian Drosten, Victor Max Corman","doi":"10.1080/22221751.2026.2673649","DOIUrl":"10.1080/22221751.2026.2673649","url":null,"abstract":"<p><p>A heart-transplant recipient developed fatal neurologic disease. High-throughput sequencing (HTS) identified Dobrava - Belgrade virus in central nervous system (CNS) and peripheral specimens. Genomic analyzes showed distinct viral populations across compartments, suggesting CNS tropism. This case highlights atypical infection presentations in immunosuppressed patients and underscores the diagnostic value of HTS.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2673649"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13224691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148053444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of ribosomal protein RPL22 restricts African swine fever virus replication by inducing PERK-dependent ER stress.","authors":"Pengfei Li, Zheyu Liao, Hua Cao, Guiqian Wang, Xinglin He, Xiaohang Li, Yumei Sun, Mengjia Zhang, Ahmed H Ghonaim, Peng Gao, Anan Jongkaewwattana, Yongtao Li, Guiqing Peng, Qigai He, Wentao Li","doi":"10.1080/22221751.2026.2678665","DOIUrl":"10.1080/22221751.2026.2678665","url":null,"abstract":"<p><p>African swine fever virus (ASFV) is a large DNA virus that causes highly lethal disease in domestic pigs and wild boars, posing a significant threat to the global swine industry. The development of effective antivirals and vaccines is hindered by the complex structure of the virus and its sophisticated manipulation of host cellular processes. The inner envelope protein p54 is essential for multiple stages of the viral life cycle, including attachment, entry, and replication. In this study, we employed TurboID-based proximity labeling to systematically map the host protein interactome of ASFV p54. Our screen identified 257 potential host interacting partners. Among these, the interaction between p54 and the 60S ribosomal protein L22 (RPL22) was validated by co-immunoprecipitation and confocal microscopy. Functional studies using an RPL22-knockout (RPL22-KO) cell line revealed that RPL22 serves as a regulatory host factor for ASFV infection. Mechanistically, the loss of RPL22 significantly inhibited ASFV replication through the activation of the PERK signaling pathway. This finding was further substantiated by pharmacological intervention; activation of the ERS/PERK pathway using thapsigargin (Tg) or CCT020312 suppressed viral replication, whereas its inhibition with 4-phenylbutyric acid (4-PBA) or AMG-PERK 44 promoted replication. In conclusion, this study presents the first systematic interactome of ASFV p54 and reveals a novel mechanism by which the host factor RPL22 modulates ASFV replication via the ERS-PERK pathway. These findings offer new insights into ASFV-host interactions and identify the ERS pathway as a promising therapeutic target for the development of anti-ASFV strategies.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2678665"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148005067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymptomatic malaria: a hidden reservoir for the transmission and circulation of artemisinin partial-resistant <i>Plasmodium falciparum</i> in Tanzania.","authors":"Ernest Mazigo, Wang-Jong Lee, Hojong Jun, Fadhila Fitriana, Jadidan Hada Syahada, Johnsy Mary Louis, Fauzi Muh, Feng Lu, Kyu Joon Lee, Joo-Won Nam, Joon-Hee Han, Tae-Hyung Kwon, Se Jin Lee, Sunghun Na, Wanjoo Chun, Won Sun Park, Eun-Taek Han, Jim Todd, Alphaxard Manjurano, Winifrida Kidima, Jin-Hee Han","doi":"10.1080/22221751.2025.2602320","DOIUrl":"10.1080/22221751.2025.2602320","url":null,"abstract":"<p><p>Asymptomatic malaria infections contribute substantially to silent transmission, but the prevalence of artemisinin resistance (ART-R) markers in these carriers remains poorly understood. A community-based cross-sectional study was conducted in Tanzania from December 2022 to July 2023, enrolling 3,489 participants from high-transmission regions of Geita and Kigoma and a low-transmission region of Arusha. Four villages per region were randomly selected, and venous blood samples were tested using rapid diagnostic tests, microscopy, and qPCR, revealing overall positivity rate of 24.4%, 15.8%, and 26.2%, respectively, which indicate a significant proportion of submicroscopic infections. Among the 802 isolates successfully sequenced for <i>pfk13</i> and <i>pfmdr1</i>, 24 (3.0%) isolates from high-transmission areas carried validated <i>pfk13</i> partial-resistance markers Y493H (0.2%), R561H (2.0%), and A675V (0.7%), while all low-transmission isolates were wild-type. All isolates retained the <i>pfmdr1</i> N86 codon, and the NFD haplotype associated with reduced susceptibility to lumefantrine was detected in 48.1% and 48.4% of isolates in high- and low-transmission areas, respectively. Mutations were more frequent in children under five and in females. Artemether-lumefantrine (AL, 64.7%) was the most commonly used antimalarial in high-transmission areas, whereas sulfadoxine-pyrimethamine (SP, 75.9%) predominated in low-transmission areas. Higher AL use correlated with increased <i>pfmdr1</i> mutation prevalence in high-transmission regions, while NFD detection in low-transmission areas may reflect gene flow from high-transmission settings. These findings demonstrate that asymptomatic carriers are a substantial hidden reservoir of ART-resistant parasites, emphasizing the importance of integrating molecular surveillance and demographic information on asymptomatic infections into malaria control programs to detect emerging resistance and guide targeted interventions in Tanzania.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2602320"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12951663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147303653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Prohibitin (PHB) as an Entry Factor of Highly Pathogenic Coronaviruses Using Metabolic Glycoengineering and Photo-Crosslinking Techniques.","authors":"Xingxing Zhu, Xiaoyang Wang, Yiming Wang, Xinchen Wang, Yuanhao Li, Liwen Hua, Honggao Duan, Rongding Yan, Tao Yuan, Jing Li, Yeshuang Yuan, Bo Zhang, Demin Zhou, Sulong Xiao","doi":"10.1080/22221751.2026.2686461","DOIUrl":"10.1080/22221751.2026.2686461","url":null,"abstract":"<p><p>Coronaviruses (CoVs) rely on host surface factors to mediate viral attachment and entry, yet conserved host cofactors shared across different CoVs remain poorly defined. Here, we established a host-factor capture platform based on metabolic glycoengineering and photo-crosslinking to identify host factors associated with CoV Spike proteins under near-physiological conditions. Using this approach, we identified prohibitin (PHB) as a candidate Spike-associated host factor shared by SARS-CoV, MERS-CoV, and SARS-CoV-2. Functional analyses showed that depletion of membrane-associated PHB decreased pseudovirus infection, whereas PHB reconstitution or overexpression enhanced susceptibility across multiple cell systems, including Huh7, BEAS-2B, Calu-3, and primary human airway epithelial cells (hAECs). In addition, antibody-mediated blockade of cell-surface PHB suppressed pseudovirus entry in both hAECs and human lung organoids. Immunohistochemical analyses further demonstrated PHB expression in normal human lung tissues. Mechanistically, recombinant PHB interacted with the Spike proteins of all three CoVs, and this association was supported by surface plasmon resonance and co-immunoprecipitation assays. Structural prediction combined with fragment-based alanine scanning identified residues 139-154 within the extracellular PHB domain as a critical region for efficient Spike association. Furthermore, PHB-associated pseudovirus entry was sensitive to lipid raft disruption, supporting a role for cholesterol-dependent membrane microdomains in this process. Together, these findings identify membrane-associated PHB as a conserved Spike-associated host factor linked to CoV entry.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2686461"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13288833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148197844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-assisted MALDI-TOF MS for identifying carbapenem resistance in clinical <i>Acinetobacter baumannii</i> isolates.","authors":"Jun-Hyeok Ham, Yeon-Jung Lee, Hae-Yeong Kim","doi":"10.1080/22221751.2026.2716498","DOIUrl":"10.1080/22221751.2026.2716498","url":null,"abstract":"<p><p>Carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) is one of the most critical public health threats worldwide due to its high infection rates, substantial mortality, and limited therapeutic choices. As CRAB infections are frequently multidrug-resistant, rapid and accurate determination of carbapenem susceptibility is essential for appropriate therapeutic decision-making. We established an integrated framework combining matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) with artificial intelligence (AI) to enable rapid prediction of carbapenem resistance in <i>A. baumannii</i>. A total of 191 clinical and surveillance isolates, including CRAB and carbapenem-susceptible <i>A. baumannii</i> (CSAB), were recovered from hospitalized patients and phenotypically characterized by standard minimum inhibitory concentration testing. MALDI-TOF MS spectra were subsequently acquired, and six AI models were developed and systematically investigated for predictive performance, among which the eXtreme Gradient Boosting (XGBoost) model achieved the highest discriminatory performance, distinguishing CRAB from CSAB with an accuracy of 96.36% and robust overall performance. Feature importance analysis of the XGBoost model revealed that its high predictive performance was driven partially by spectral features associated with horizontal gene transfer-related elements and membrane and transport-associated proteins, providing candidate molecular correlates of carbapenem resistance. Overall, these results show that AI-assisted MALDI-TOF MS enables rapid and accurate prediction of carbapenem resistance in <i>A. baumannii</i> and provides insights into the molecular features associated with resistance acquisition.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":"15 1","pages":"2716498"},"PeriodicalIF":7.0,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13520854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}