Emerging Microbes & Infections最新文献

{"title":"Differential replication, pathology, and immune response of clade IIb mpox virus in C57BL/6 and BALB/c mice.","authors":"Meimei Duan, Xiangmei Zhang, Xian Tang, Zhuohuan Li, Wenqi Huang, Qi Chen, Mingxia Zhang, Lin Cheng, Zheng Zhang","doi":"10.1080/22221751.2025.2479043","DOIUrl":"10.1080/22221751.2025.2479043","url":null,"abstract":"<p><p>We have previously established a clade IIb mpox virus (MPXV) pathogenic BALB/c mouse model for developing mpox countermeasures. Here, we comprehensively investigated the susceptibility of BALB/c and C57BL/6 mice to MPXV and found that Clade IIb MPXV was capable of rapid replication in the lungs of both mouse strains, thus triggering similar dynamic pathological changes and antibody responses. However, C57BL/6 mice, compared to BALB/c mice, seem less susceptible to MPXV, evidenced by no significant weight loss, lower viral load, faster viral clearance, and earlier pathological improvement, as well as weaker antibody response. Interestingly, C57BL/6 <i>stat1</i>^-/- mice intranasally infected with MPXV displayed a significant body weight loss, indicating the crucial role of innate immunity in the susceptibility to MPXV. The C57BL/6 model mimics clinical characteristics of asymptomatic or mildly symptomatic patients with mild mpox, which will be beneficial for exploring MPXV infection, transmission, pathogenesis, and immune responses.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2479043"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nationwide seroprevalence of SARS-CoV-2 Delta variant and five Omicron sublineages in companion cats and dogs in the USA: insights into their role in COVID-19 epidemiology.","authors":"Subarna Barua, Nneka Vivian Iduu, Daniel Felipe Barrantes Murillo, Asfiha Tarannum, Hill Dimino, Suchita Barua, Yue Shu, Calvin Johnson, Megan R Miller, Kelly Chenoweth, Peter Christopherson, Laura Huber, Theresa Wood, Kelley Turner, Chengming Wang","doi":"10.1080/22221751.2024.2437246","DOIUrl":"10.1080/22221751.2024.2437246","url":null,"abstract":"<p><p>Understanding SARS-CoV-2 epidemiology in companion animals is critical for evaluating their role in viral transmission and their potential as sentinels for human infections. This large-scale serosurvey analyzed serum samples from 706 cats and 2,396 dogs collected across the USA in 2023 using a surrogate virus neutralization test (sVNT) to detect SARS-CoV-2 antibodies. Overall, 5.7% of cats and 4.7% of dogs tested positive for antibodies, with younger animals (under 12 months) showing significantly lower seropositivity rates (<i>p </i>= 0.0048). Additionally, we analyzed 153 positive samples for variant-specific antibody responses using six sVNT kits targeting the Delta variant and five Omicron sublineages. Among cats, 67.5% showed antibodies to Delta, with positivity rates for Omicron sublineages as follows: BA.1 (62.5%), BA.2 (42.5%), BA.4/BA.5 (77.5%), XBB (52.5%), and XBB.1.5 (45.0%). In dogs, 55.8% were positive for Delta, and Omicron sublineage rates were BA.1 (46.0%), BA.4/BA.5 (37.2%), XBB (58.4%), BA.2 (13.3%), and XBB.1.5 (9.7%). Given the close contact between companion animals and humans, and the persistence of antibodies against various SARS-CoV-2 variants and sublineages, our findings suggest that seroprevalence in cats and dogs may serve as valuable tool for tracking COVID-19 epidemiology.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2437246"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unprecedented large outbreak of <i>Plasmodium malariae</i> malaria in Vietnam: Epidemiological and clinical perspectives.","authors":"Chau Van Khanh, Hương Giang Lê, Tuấn Cường Võ, Nguyen Xuan Quang, Do Van Nguyen, Nguyen Cong Trung Dung, Le Thanh Tam, Nguyen Thanh Thuy Nhien, Đăng Thùy Dương Nguyễn, Thu Hằng Nguyễn, Tran Thi Hue Van, Le Duc Vinh, Pham Minh Quan, Nguyen Kim Trung, Jung-Mi Kang, Byoung-Kuk Na, Huynh Hong Quang","doi":"10.1080/22221751.2024.2432359","DOIUrl":"10.1080/22221751.2024.2432359","url":null,"abstract":"<p><p><i>Plasmodium malariae</i>, a causative agent of quartan malaria, is prevalent across tropical and subtropical regions, but global cases have been usually very rare and sporadic. However, a significant outbreak of quartan malaria caused by <i>P. malariae</i> occurred in Khanh Vinh District, Khanh Hoa Province, Vietnam in 2023 and the outbreak persists. In this report, we present the epidemiological and clinical characteristics of this unprecedented outbreak of quartan malaria in Vietnam.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2432359"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11650441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly pathogenic avian H5N1 influenza A virus replication in ex vivo cultures of bovine mammary gland and teat tissues.","authors":"Masaki Imai, Hiroshi Ueki, Mutsumi Ito, Kiyoko Iwatsuki-Horimoto, Maki Kiso, Asim Biswas, Sanja Trifkovic, Nigel Cook, Peter J Halfmann, Gabriele Neumann, Amie J Eisfeld, Yoshihiro Kawaoka","doi":"10.1080/22221751.2025.2450029","DOIUrl":"10.1080/22221751.2025.2450029","url":null,"abstract":"<p><p>Since early 2024, highly pathogenic avian influenza H5N1 viruses have been causing outbreaks in dairy cattle in the United States. Here, we compared the replicative capacity of A/dairy cattle/Texas/24-008749-001/2024 (H5N1; Cow-H5N1) isolated from a dairy cow, A/chicken/Ghana/AVL-76321VIR7050-39/2021 (H5N1; Chicken-H5N1) isolated from a chicken, and a human H1N1 2009 pandemic virus in ex vivo explant cultures of mammary gland and teat from lactating cows. We also examined the expression of influenza virus receptors in these organs. We observed that human influenza virus receptors are widely distributed throughout the epithelium of alveoli, ducts, and gland cisterns within the mammary gland, and in the teat cistern epithelium of dairy cattle, whereas avian influenza virus receptors are distributed on the alveolar, ductal, and teat cistern epithelium. We also found that Cow-H5N1 virus replicates more efficiently than Chicken-H5N1 or human H1N1pdm viruses in the gland cistern epithelium of dairy cattle. Notably, bovine H5N1 viruses replicated efficiently in the epithelium of the bovine teat cistern. These findings suggest that H5N1 viruses invade the mammary gland through the teat canal, which is easily accessed by viruses.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2450029"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11740294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influenza H5Nx viruses are susceptible to MEK1/2 inhibition by zapnometinib.","authors":"André Schreiber, Nicole Oberberg, Benjamin Ambrosy, Franziska Rodner, Sriram Kumar, Duygu Merve Caliskan, Linda Brunotte, Martin Beer, Stephan Ludwig","doi":"10.1080/22221751.2025.2471022","DOIUrl":"10.1080/22221751.2025.2471022","url":null,"abstract":"<p><p>Highly pathogenic avian influenza A viruses (HPAIV) pose a significant threat to both animal and human health. These viruses have the potential to cause severe respiratory and systemic infections in birds and several mammalian species. The recent global outbreak of the H5N1 clade 2.3.4.4b spread in wild and domestic birds is now considered to be a panzoonosis. Spillover events in dairy cattle farms in the U.S. have highlighted the urgent need for effective antiviral therapies, especially in view of human infections. This study investigates the selective MEK1/2 inhibitor zapnometinib (ZMN) as a potential antiviral agent against HPAIVs. Our <i>in vitro</i> experiments demonstrate that ZMN significantly impairs viral replication across multiple HPAIV strains, including H5N1 clade 2.3.4.4b in cell lines and primary bronchial epithelial cells. The mechanism of action is based on the nuclear retention of newly produced viral ribonucleoprotein complexes (vRNP), when the MEK/ERK/RSK1 kinase cascade is inhibited. We furthermore could show, that ZMN not only acts antiviral in a standalone treatment but has synergistic potential when used in combination with direct-acting antivirals like oseltamivir or baloxavir. Therefore, ZMN treatment offers a promising strategy for future antiviral development.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2471022"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the infection: mapping the risk of cardiovascular events post-scrub typhus in a nationwide cohort study.","authors":"Jih-Kai Yeh, Victor Chien-Chia Wu, Shao-Wei Chen, Chia-Ling Wu, Yu-Sheng Lin, Chun-Wen Cheng, Chih-Hsiang Chang, Michael Wu, Pao-Hsien Chu, Shang-Hung Chang, Yu-Tung Huang","doi":"10.1080/22221751.2025.2467766","DOIUrl":"10.1080/22221751.2025.2467766","url":null,"abstract":"<p><p><b>ABSTRACT</b>Scrub typhus, caused by <i>Orientia tsutsugamushi</i>, often involves multiple organs, but its cardiovascular (CV) sequelae in survivors remain under-researched. This retrospective cohort study analyzed data from the National Health Insurance Research Database (NHIRD) spanning 2010-2015 to assess CV risks among scrub typhus survivors. Excluding those with prior CV events, we focused on outcomes such as acute myocardial infarction (AMI), heart failure hospitalization (HFH), strokes, new-onset atrial fibrillation (AF), aortic aneurysm or dissection, venous thromboembolism (VTE), and CV death. From 2,269 scrub typhus patients without previous CV events (mean age 47.8 ± 16.1; 38.0% female), and a matched control group (<i>n</i> = 2,264), we observed a higher incidence of HFH, new-onset AF, and total CV events in the scrub typhus cohort. Adjusted hazard ratios (aHRs) were 1.97 (95% CI: 1.13-3.42) for HFH, 2.48 (95% CI: 1.23-5.0) for new-onset AF, and 1.43 (95% CI: 1.08-1.91) for total CV events. Other outcomes did not significantly differ. Scrub typhus survivors exhibit an increased risk of CV events, particularly HFH and new-onset AF, underscoring the importance of heightened physician awareness and post-infection cardiac surveillance.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2467766"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143406476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaccine effectiveness dynamics against influenza and SARS-CoV-2 in community-tested patients in France 2023-2024.","authors":"Claire Nour Abou Chakra, François Blanquart, Vincent Vieillefond, Vincent Enouf, Benoit Visseaux, Stéphanie Haim-Boukobza, Laurence Josset, Marie-Anne Rameix-Welti, Bruno Lina, Marta C Nunes, Antonin Bal","doi":"10.1080/22221751.2025.2466699","DOIUrl":"10.1080/22221751.2025.2466699","url":null,"abstract":"<p><p>The epidemiology of respiratory viruses and vaccine effectiveness (VE) in the community are not well described. This study assessed VE against a positive test of influenza (VEf) and SARS-CoV-2 (VECov). Data from networks of community-based laboratories in France were collected during standard of care in the 2023-2024 epidemic season (<i>n</i> = 511,083 multiplex RT-PCR tests). Patients' demographics and symptoms were reported in addition to viral sequencing results. The test-negative design was used to estimate VEf and VECov by time since vaccination and calendar week. Adjusted VEf by age, sex, presence of symptoms, PCR technique, and week of testing, was 47.6% (95% CI: 44.3-50.7%). VEf was lower in patients ≥65 years (42.0%; 95% CI: 36.6-46.9%) than in 18-64 years (52.9%; 95% CI: 48.6-56.8%). The adjusted VEf against type A influenza, which represented 98% of typed viruses, was 51% (45%-56.6%) for patients vaccinated 15 days to 3 months before testing, and 35.5% (24.2%-45.3%) for those vaccinated 3-6 months before testing. For VECov, the adjusted estimate in patients vaccinated 15 days to 3 months prior to testing was 40.6% (7.2%-58.6%) at week 39, 24.8% (4.0%-38.8%) at week 45, and dropped systematically through the epidemic season as the JN.1 variant became dominant. This study showed moderate VEf and VECov against infection in the community and highlighted the impact of time since vaccination and age for both estimates, and the new variant emergence on VECov. These findings should be considered in future vaccination campaigns.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2466699"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sepsis-induced inflammasome impairment facilitates development of secondary <i>A. baumannii</i> pneumonia.","authors":"Aldona Jeznach, Karolina Sidor-Dzitkowska, Magdalena Bandyszewska, Małgorzata Grzanka, Piotr Popławski, Anna Marszalik, Joanna Domagała-Kulawik, Radosław Stachowiak, Grażyna Hoser, Tomasz Skirecki","doi":"10.1080/22221751.2025.2492206","DOIUrl":"10.1080/22221751.2025.2492206","url":null,"abstract":"<p><strong>Background: </strong><i>Acinetobacter baumannii</i> has become one of the most critical pathogens causing nosocomial pneumonia. Existing animal models of <i>A. baumannii</i> pneumonia are not relevant to the majority of critical care patients. We aimed to develop a novel model of secondary <i>A. baumannii</i> pneumonia in post-sepsis mice.</p><p><strong>Methods: </strong>A two-hit model of sepsis induced by cecal ligation and puncture followed by <i>A. baumannii</i> pneumonia on day 5 was established. In addition, the two-hit model was established in humanized mice. A period of 2 h of mechanical ventilation followed by observation was used in additional experiments. Lung histopathology, bacterial cultures, and cellular infiltration were analysed as well as markers of the inflammasome activity <i>in vivo</i> and <i>ex vivo</i>.</p><p><strong>Results: </strong><i>A. baumannii</i> infection caused mortality and loss of body weight and temperature in post-sepsis mice. Increased lung bacterial burden and dissemination together with signs of enhanced inflammatory injury were observed in post-sepsis mice but not control mice that were challenged with <i>A. baumannii</i>. Post-sepsis mice were unable to mount inflammasome activation in response to secondary pneumonia to the level of control mice. Transfer of wild-type but not capsase-1 KO alveolar macrophages was able to restore the pulmonary protection against <i>A. baumannii</i>. Mechanical ventilation exacerbated the pathological response to pneumonia in post-sepsis mice but enhanced inflammasome signalling in non-sepsis mice with pneumonia.</p><p><strong>Conclusions: </strong>We established a novel model of <i>A. baumannii</i> pneumonia that revealed sepsis-induced impairment of inflammasome activation in alveolar macrophages is critical for the control of secondary <i>A. baumannii</i> pneumonia.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2492206"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SREBP2-dependent lipid droplet formation enhances viral replication and deteriorates lung injury in mice following IAV infection.","authors":"Xinsen Li, Lu Li, Jijing Tian, Ruijing Su, Jiali Sun, Yuli Li, Lige Wang, Hongye Zhou, Shuhan Sha, Jin Xiao, Hong Dong, Caiyun Huo, Yanxin Hu, Hanchun Yang","doi":"10.1080/22221751.2025.2470371","DOIUrl":"10.1080/22221751.2025.2470371","url":null,"abstract":"<p><p>Influenza A virus (IAV) is a significant zoonotic pathogen that poses a considerable challenge to public health due to its continuous mutations. Lipid droplets (LDs) have been shown to play an important role in the process of several viral infections. However, their role in IAV infection remains unclear. Here, we found that IAV infection altered the lipid metabolism and increased the content of LDs in the lungs of mice. In vitro, IAV infection also mediated the formation of LDs in A549 cells. Besides, inhibition of the formation of lipid droplets can significantly suppress IAV replication and the release of inflammatory factors, indicating that LDs could facilitate the virus replication and inflammatory response. Furthermore, we discovered that IAV infection could activate the SREBP2, a crucial lipid-regulating transcription factor that regulates the expressions of downstream proteins named HMGCR and HMGCS. HMGCR and HMGCS involved in the process of cholesterol synthesis, which further promoted the formation of LDs. Additionally, the use of fatostatin that specifically inhibits the maturation of SREBP2 was able to significantly suppress the viral replication of H5N1 in cells and effectively ameliorated IAV-induced lung injury in mice, which eventually promoted the survival rate of infected mice. Taken together, we demonstrate the essential roles of lipid metabolism and LD formation in IAV replication and pathogenesis, which may better facilitate the advancement of new strategies against IAV infection, especially the highly pathogenic H5N1 virus.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2470371"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eukaryotic RNA binding protein hnRNPH1 suppresses influenza A virus replication through interaction with virus NS1 protein.","authors":"Jinyu Wang, Yang Zhang, Lishan Sun, Zihan Wang, Cui Hao, Wei Wang","doi":"10.1080/22221751.2025.2477645","DOIUrl":"10.1080/22221751.2025.2477645","url":null,"abstract":"<p><p>The NS1 protein of influenza A virus (IAV) is a multi-functional protein which can antagonize host immune system and facilitate viral replication by interacting with host factors. However, the novel partners in host cells interacting with NS1 need to be fully elucidated. In the current study, we identified hnRNPH1 as a novel binding partner of NS1 to regulate IAV replication. Notably, overexpression of hnRNPH1 decreased IAV multiplication, while knockdown of hnRNPH1 enhanced IAV replication. hnRNPH1 can interact with NS1 to change the intracellular localization and splicing function of NS1, and impact IAV replication through interacting with p53 to regulate cell apoptosis. In addition, the RBD domain of NS1 and the RRM and NLS regions of hnRNPH1 may be the major sites for their interaction. In summary, our studies identified hnRNPH1 as a novel NS1-binding protein and elucidated its regulatory roles in IAV replication, which will provide new insights into the roles of NS1 binding proteins, and give a reference for anti-IAV therapy based on NS1-host interaction.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2477645"},"PeriodicalIF":8.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}