Differential replication, pathology, and immune response of clade IIb mpox virus in C57BL/6 and BALB/c mice.

IF 7.5 2区医学 Q1 IMMUNOLOGY

Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI:10.1080/22221751.2025.2479043

Meimei Duan, Xiangmei Zhang, Xian Tang, Zhuohuan Li, Wenqi Huang, Qi Chen, Mingxia Zhang, Lin Cheng, Zheng Zhang

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引用次数: 0

Abstract

We have previously established a clade IIb mpox virus (MPXV) pathogenic BALB/c mouse model for developing mpox countermeasures. Here, we comprehensively investigated the susceptibility of BALB/c and C57BL/6 mice to MPXV and found that Clade IIb MPXV was capable of rapid replication in the lungs of both mouse strains, thus triggering similar dynamic pathological changes and antibody responses. However, C57BL/6 mice, compared to BALB/c mice, seem less susceptible to MPXV, evidenced by no significant weight loss, lower viral load, faster viral clearance, and earlier pathological improvement, as well as weaker antibody response. Interestingly, C57BL/6 stat1^-/- mice intranasally infected with MPXV displayed a significant body weight loss, indicating the crucial role of innate immunity in the susceptibility to MPXV. The C57BL/6 model mimics clinical characteristics of asymptomatic or mildly symptomatic patients with mild mpox, which will be beneficial for exploring MPXV infection, transmission, pathogenesis, and immune responses.

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进化支IIb mpox病毒在C57BL/6和BALB/c小鼠中的差异复制、病理和免疫应答

我们之前已经建立了一个进化支Ⅱb型m痘病毒（MPXV）致病性BALB/c小鼠模型，用于开发m痘对策。在此，我们全面研究了BALB/c和C57BL/6小鼠对MPXV的易感性，发现Clade IIb MPXV能够在两种小鼠菌株的肺部快速复制，从而引发相似的动态病理变化和抗体反应。然而，与BALB/c小鼠相比，C57BL/6小鼠似乎对MPXV不太敏感，表现为体重没有明显减轻，病毒载量更低，病毒清除更快，病理改善更早，抗体反应更弱。有趣的是，经鼻内感染MPXV的C57BL/6 stat1-/-小鼠表现出明显的体重减轻，这表明先天免疫在MPXV易感性中起着至关重要的作用。C57BL/6模型模拟了无症状或轻度症状的轻度m痘患者的临床特征，有助于探索MPXV的感染、传播、发病机制和免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY

CiteScore

26.20

自引率

2.30%

发文量

276

审稿时长

20 weeks

期刊介绍： Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.