IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70129

Tao Liu, Mengmin Li, Fengqin Feng, Yuanxiang Jin, Zhibin Tian, Bokai Wan, Jintao Cheng, Shiyu Li, Qingchi Wang, Chaozhi Wei, Dou Wang, Kan Shao, Ting Luo, Guiling Yang

{"title":"Natural Synergistic Anti-Fatigue Formula Development via Zebrafish Model and Combination Index Method","authors":"Tao Liu, Mengmin Li, Fengqin Feng, Yuanxiang Jin, Zhibin Tian, Bokai Wan, Jintao Cheng, Shiyu Li, Qingchi Wang, Chaozhi Wei, Dou Wang, Kan Shao, Ting Luo, Guiling Yang","doi":"10.1002/efd2.70129","DOIUrl":"10.1002/efd2.70129","url":null,"abstract":"Many plant and fungal extracts (PFEs) show anti-fatigue properties through various pathways, but synergistic formulas and mechanisms are unclear. We screened six PFEs using a sodium sulfite (Na2SO3)-induced exercise fatigue zebrafish model and developed a synergistic anti-fatigue formula based on the combination index (CI) method. Red ginseng (RG), Lycium ruthenicum (LR) and Ganoderma lucidum (GL) extracts were selected as formulation materials based on their potential synergistic possibilities. The formula demonstrated a strong synergistic effect on recovery rate with a CI of 0.04 when RG, LR, and GL were mixed at concentrations of 3.6, 4.0, and 14 μg/mL, respectively. Ginsenosides Re, LR anthocyanins and GL polysaccharides are the main anti-fatigue components. The contents of ATP and SOD were increased by 39.1% and 53.9%, while lactic acid and malondialdehyde (MDA) contents were decreased by 31.3% and 15.6%. Zebrafish swimming ability was restored by 97.6% relative to the control group, demonstrating a fatigue recovery effect comparable to that of Red Bull. The formula exerts anti-fatigue effects by regulating muscle contraction, energy metabolism and oxidative stress at the relative rRNA expression. These findings highlight the promising potential of rationally designed PFEs combinations for functional food.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Banana Biomass: Nutritional and Phytochemical Insights With Applications in Food and Allied Industries 香蕉生物量的增值：营养和植物化学的见解与应用在食品和相关工业

IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70143

Bushra Iram Fatima, Saeed Akhtar, Muhammad Zulqarnain Khan, Muhammad Qamar, Tariq Ismail, Wisha Saeed, Elham Assadpour, Tuba Esatbeyoglu, Seid Mahdi Jafari

{"title":"Valorization of Banana Biomass: Nutritional and Phytochemical Insights With Applications in Food and Allied Industries","authors":"Bushra Iram Fatima, Saeed Akhtar, Muhammad Zulqarnain Khan, Muhammad Qamar, Tariq Ismail, Wisha Saeed, Elham Assadpour, Tuba Esatbeyoglu, Seid Mahdi Jafari","doi":"10.1002/efd2.70143","DOIUrl":"10.1002/efd2.70143","url":null,"abstract":"The global banana processing industry generates substantial organic waste in the form of peels, leaves, stems, and flowers, posing both environmental challenges and opportunities for valorization within a circular economy framework. This review provides a critical and integrative synthesis of the recent literature on nutritional composition, phytochemical diversity, pharmacological potential, food, and non-food applications of various bananas parts with a dedicated focus on safety/toxicity, sustainability, and industrial feasibility. Evidence indicates that banana peels and other underutilized parts are rich in primary and secondary metabolites, supporting their application in functional foods, edible packaging films, animal feed, and also as biofertilizers. Green extraction approaches using ethanol and water emerge as suitable techniques for food grade applications, while organic solvents such as methanol and ethyl acetate for cosmeceutical and pharmaceutical industry. However, the available evidence is primarily based on in vitro studies, animal modeling, and pilot-scale processing. This review concludes that while sustainable upcycling of banana by-products is scientifically justified, it remains translationally limited, necessitating rigorous clinical trials and comprehensive techno-economic evaluations to enable large scale industrial adoption.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of Banana Biomass: Nutritional and Phytochemical Insights With Applications in Food and Allied Industries 香蕉生物量的增值：营养和植物化学的见解与应用在食品和相关工业

IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70143

Bushra Iram Fatima, Saeed Akhtar, Muhammad Zulqarnain Khan, Muhammad Qamar, Tariq Ismail, Wisha Saeed, Elham Assadpour, Tuba Esatbeyoglu, Seid Mahdi Jafari

{"title":"Valorization of Banana Biomass: Nutritional and Phytochemical Insights With Applications in Food and Allied Industries","authors":"Bushra Iram Fatima, Saeed Akhtar, Muhammad Zulqarnain Khan, Muhammad Qamar, Tariq Ismail, Wisha Saeed, Elham Assadpour, Tuba Esatbeyoglu, Seid Mahdi Jafari","doi":"10.1002/efd2.70143","DOIUrl":"https://doi.org/10.1002/efd2.70143","url":null,"abstract":"The global banana processing industry generates substantial organic waste in the form of peels, leaves, stems, and flowers, posing both environmental challenges and opportunities for valorization within a circular economy framework. This review provides a critical and integrative synthesis of the recent literature on nutritional composition, phytochemical diversity, pharmacological potential, food, and non-food applications of various bananas parts with a dedicated focus on safety/toxicity, sustainability, and industrial feasibility. Evidence indicates that banana peels and other underutilized parts are rich in primary and secondary metabolites, supporting their application in functional foods, edible packaging films, animal feed, and also as biofertilizers. Green extraction approaches using ethanol and water emerge as suitable techniques for food grade applications, while organic solvents such as methanol and ethyl acetate for cosmeceutical and pharmaceutical industry. However, the available evidence is primarily based on in vitro studies, animal modeling, and pilot-scale processing. This review concludes that while sustainable upcycling of banana by-products is scientifically justified, it remains translationally limited, necessitating rigorous clinical trials and comprehensive techno-economic evaluations to enable large scale industrial adoption.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astragalus Polysaccharide Improved DSS-Induced Colitis in Mice By Remodeling the Gut Microbiota 黄芪多糖通过重塑肠道菌群改善dss诱导的小鼠结肠炎

IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70141

Israr Khan, Yanrui Bai, Junxiang Li, Ziyuan Wang, Zhiming Xu, Tao Yuan, Xiangyuan Liu, Yu Zhang, Chunjiang Zhang, Shu Xu

{"title":"Astragalus Polysaccharide Improved DSS-Induced Colitis in Mice By Remodeling the Gut Microbiota","authors":"Israr Khan, Yanrui Bai, Junxiang Li, Ziyuan Wang, Zhiming Xu, Tao Yuan, Xiangyuan Liu, Yu Zhang, Chunjiang Zhang, Shu Xu","doi":"10.1002/efd2.70141","DOIUrl":"https://doi.org/10.1002/efd2.70141","url":null,"abstract":"Gut microbiota has become a key therapeutic target for inflammatory bowel disease (IBD). Astragalus polysaccharides (AP), the main active components of Astragalus membranaceus, can prevent experimental colitis, but their mechanisms remain unclear. This study investigated the therapeutic effect of AP on DSS-induced colitis and its mechanism of attenuation. AP significantly improved colitis by increasing body weight and colon length, reducing histological injury, and lowering the disease activity index (DAI). AP inhibited proinflammatory cytokines (IL-6, IL-17A, IL-22, IL-23), upregulated anti-inflammatory cytokines (IL-10, TGF-β) in colon tissue, and increased fecal SCFA levels. AP also reshaped gut microbiota by decreasing Proteobacteria, Verrucomicrobia, Allobaculum, Turicibacter, and Akkermansia, while enriching Firmicutes, Bacteroidetes, Lactobacillus, Lachnospiraceae, Ruminococcus, and Oscillospira. These enriched genera positively correlated with IL-10, TGF-β, and SCFAs, and negatively with IL-6, IL-17A, and IL-23. KEGG analysis showed that AP restored metabolic pathways disrupted during colitis. Overall, AP protected against DSS-induced colitis by modulating gut microbial composition, metabolism, and immune responses. Importantly, ABX + AP experiments confirmed that the therapeutic effect of AP depends on gut microbiota. These findings indicate that AP alleviates colitis by remodeling the gut microbiota and may serve as a promising microbiome-based therapy for ulcerative colitis.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Synergistic Anti-Fatigue Formula Development via Zebrafish Model and Combination Index Method 基于斑马鱼模型和组合指数法的天然协同抗疲劳配方开发

IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70129

Tao Liu, Mengmin Li, Fengqin Feng, Yuanxiang Jin, Zhibin Tian, Bokai Wan, Jintao Cheng, Shiyu Li, Qingchi Wang, Chaozhi Wei, Dou Wang, Kan Shao, Ting Luo, Guiling Yang

{"title":"Natural Synergistic Anti-Fatigue Formula Development via Zebrafish Model and Combination Index Method","authors":"Tao Liu, Mengmin Li, Fengqin Feng, Yuanxiang Jin, Zhibin Tian, Bokai Wan, Jintao Cheng, Shiyu Li, Qingchi Wang, Chaozhi Wei, Dou Wang, Kan Shao, Ting Luo, Guiling Yang","doi":"10.1002/efd2.70129","DOIUrl":"https://doi.org/10.1002/efd2.70129","url":null,"abstract":"Many plant and fungal extracts (PFEs) show anti-fatigue properties through various pathways, but synergistic formulas and mechanisms are unclear. We screened six PFEs using a sodium sulfite (Na2SO3)-induced exercise fatigue zebrafish model and developed a synergistic anti-fatigue formula based on the combination index (CI) method. Red ginseng (RG), Lycium ruthenicum (LR) and Ganoderma lucidum (GL) extracts were selected as formulation materials based on their potential synergistic possibilities. The formula demonstrated a strong synergistic effect on recovery rate with a CI of 0.04 when RG, LR, and GL were mixed at concentrations of 3.6, 4.0, and 14 μg/mL, respectively. Ginsenosides Re, LR anthocyanins and GL polysaccharides are the main anti-fatigue components. The contents of ATP and SOD were increased by 39.1% and 53.9%, while lactic acid and malondialdehyde (MDA) contents were decreased by 31.3% and 15.6%. Zebrafish swimming ability was restored by 97.6% relative to the control group, demonstrating a fatigue recovery effect comparable to that of Red Bull. The formula exerts anti-fatigue effects by regulating muscle contraction, energy metabolism and oxidative stress at the relative rRNA expression. These findings highlight the promising potential of rationally designed PFEs combinations for functional food.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astragalus Polysaccharide Improved DSS-Induced Colitis in Mice By Remodeling the Gut Microbiota 黄芪多糖通过重塑肠道菌群改善dss诱导的小鼠结肠炎

IF 5.7

eFood Pub Date : 2026-03-23 DOI: 10.1002/efd2.70141

Israr Khan, Yanrui Bai, Junxiang Li, Ziyuan Wang, Zhiming Xu, Tao Yuan, Xiangyuan Liu, Yu Zhang, Chunjiang Zhang, Shu Xu

{"title":"Astragalus Polysaccharide Improved DSS-Induced Colitis in Mice By Remodeling the Gut Microbiota","authors":"Israr Khan, Yanrui Bai, Junxiang Li, Ziyuan Wang, Zhiming Xu, Tao Yuan, Xiangyuan Liu, Yu Zhang, Chunjiang Zhang, Shu Xu","doi":"10.1002/efd2.70141","DOIUrl":"10.1002/efd2.70141","url":null,"abstract":"Gut microbiota has become a key therapeutic target for inflammatory bowel disease (IBD). Astragalus polysaccharides (AP), the main active components of Astragalus membranaceus, can prevent experimental colitis, but their mechanisms remain unclear. This study investigated the therapeutic effect of AP on DSS-induced colitis and its mechanism of attenuation. AP significantly improved colitis by increasing body weight and colon length, reducing histological injury, and lowering the disease activity index (DAI). AP inhibited proinflammatory cytokines (IL-6, IL-17A, IL-22, IL-23), upregulated anti-inflammatory cytokines (IL-10, TGF-β) in colon tissue, and increased fecal SCFA levels. AP also reshaped gut microbiota by decreasing Proteobacteria, Verrucomicrobia, Allobaculum, Turicibacter, and Akkermansia, while enriching Firmicutes, Bacteroidetes, Lactobacillus, Lachnospiraceae, Ruminococcus, and Oscillospira. These enriched genera positively correlated with IL-10, TGF-β, and SCFAs, and negatively with IL-6, IL-17A, and IL-23. KEGG analysis showed that AP restored metabolic pathways disrupted during colitis. Overall, AP protected against DSS-induced colitis by modulating gut microbial composition, metabolism, and immune responses. Importantly, ABX + AP experiments confirmed that the therapeutic effect of AP depends on gut microbiota. These findings indicate that AP alleviates colitis by remodeling the gut microbiota and may serve as a promising microbiome-based therapy for ulcerative colitis.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways 余甘子通过多种途径发挥口腔癌的治疗潜力

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70142

Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu

{"title":"Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways","authors":"Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu","doi":"10.1002/efd2.70142","DOIUrl":"10.1002/efd2.70142","url":null,"abstract":"Oral cancer represents a significant global health burden owing to the limitations of current therapeutic approaches, including adverse effects and drug resistance. Phytochemicals have gained increasing attention as promising anticancer agents with multi-target effects and low toxicity. This study evaluated the anticancer potential of Phyllanthus emblica water extract (PEWE) using in vitro and in vivo models. In vitro, PEWE exhibited dose-dependent inhibition of DOK and OECM-1 oral cancer cell proliferation while sparing non-cancerous CGHNK2 cells. PEWE significantly suppressed cell migration and invasion, induced S-phase cell cycle arrest, and promoted apoptosis associated with mitochondrial dysfunction. Morphological and molecular analyses confirmed the induction of autophagy as evidenced by increased LC3 expression, decreased p62 levels, and upregulation of Rab7. RNA sequencing revealed significant modulation of key autophagy-related genes, including ITPR1, AMBRA1, and MAP1LC3B. In vivo, PEWE effectively inhibited tumor growth in xenograft and carcinogen-induced mouse oral cancer models without any apparent systemic toxicity. These findings demonstrate that PEWE exerts anticancer effects through a coordinated mechanism involving cell cycle disruption, apoptosis, and autophagy, highlighting its potential as a novel and less toxic therapeutic agent for oral cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets 用三种酶靶点的亲和超滤技术研究了长尾蛇葡萄中潜在的降血糖、降血脂和降尿酸的生物活性成分

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70144

Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo

{"title":"Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets","authors":"Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo","doi":"10.1002/efd2.70144","DOIUrl":"10.1002/efd2.70144","url":null,"abstract":"Hyperuricemia demonstrates synergistic interactions in the progression of hyperlipidemia and hyperglycemia. There is a pressing demand to explore natural products with negligible adverse effects for the prevention and regulation of hyperuricemia, particularly when combined with hyperglycemia and hyperlipidemia. Ampelopsis grossedentata has been traditionally employed as a functional food throughout an extensive historical duration. Nevertheless, the bioactive compounds that contribute to its hypoglycemic, hypolipidemic, and hypouricemic effects, as well as the molecular mechanisms involved, have not yet been completely clarified. This study initially conducted in vitro assays to evaluate the inhibitory activity of A. grossedentata towards α-glucosidase, pancreatic lipase, and xanthine oxidase. The results revealed that the A. grossedentata extract exhibited substantial inhibitory activities against these enzyme targets. Subsequently, the four most promising active compounds (quercetin, myricetrin, dihydromyricetin and taxifolin) were screened and characterized by a bioaffinity ultrafiltration method combined with LC-MS/MS analysis. Further validation of these bioactive compounds was achieved through molecular docking studies. Additionally, an interaction network was established to elucidate the mechanisms of action between the candidate constituents and their respective targets. The results indicate that A. grossedentata holds great promise as a potential natural therapeutic functional food for managing diabetes, overweight, and hyperuricemia in the foreseeable future.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways 余甘子通过多种途径发挥口腔癌的治疗潜力

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70142

Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu

{"title":"Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways","authors":"Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu","doi":"10.1002/efd2.70142","DOIUrl":"https://doi.org/10.1002/efd2.70142","url":null,"abstract":"Oral cancer represents a significant global health burden owing to the limitations of current therapeutic approaches, including adverse effects and drug resistance. Phytochemicals have gained increasing attention as promising anticancer agents with multi-target effects and low toxicity. This study evaluated the anticancer potential of Phyllanthus emblica water extract (PEWE) using in vitro and in vivo models. In vitro, PEWE exhibited dose-dependent inhibition of DOK and OECM-1 oral cancer cell proliferation while sparing non-cancerous CGHNK2 cells. PEWE significantly suppressed cell migration and invasion, induced S-phase cell cycle arrest, and promoted apoptosis associated with mitochondrial dysfunction. Morphological and molecular analyses confirmed the induction of autophagy as evidenced by increased LC3 expression, decreased p62 levels, and upregulation of Rab7. RNA sequencing revealed significant modulation of key autophagy-related genes, including ITPR1, AMBRA1, and MAP1LC3B. In vivo, PEWE effectively inhibited tumor growth in xenograft and carcinogen-induced mouse oral cancer models without any apparent systemic toxicity. These findings demonstrate that PEWE exerts anticancer effects through a coordinated mechanism involving cell cycle disruption, apoptosis, and autophagy, highlighting its potential as a novel and less toxic therapeutic agent for oral cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets 用三种酶靶点的亲和超滤技术研究了长尾蛇葡萄中潜在的降血糖、降血脂和降尿酸的生物活性成分

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70144

Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo

{"title":"Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets","authors":"Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo","doi":"10.1002/efd2.70144","DOIUrl":"https://doi.org/10.1002/efd2.70144","url":null,"abstract":"Hyperuricemia demonstrates synergistic interactions in the progression of hyperlipidemia and hyperglycemia. There is a pressing demand to explore natural products with negligible adverse effects for the prevention and regulation of hyperuricemia, particularly when combined with hyperglycemia and hyperlipidemia. Ampelopsis grossedentata has been traditionally employed as a functional food throughout an extensive historical duration. Nevertheless, the bioactive compounds that contribute to its hypoglycemic, hypolipidemic, and hypouricemic effects, as well as the molecular mechanisms involved, have not yet been completely clarified. This study initially conducted in vitro assays to evaluate the inhibitory activity of A. grossedentata towards α-glucosidase, pancreatic lipase, and xanthine oxidase. The results revealed that the A. grossedentata extract exhibited substantial inhibitory activities against these enzyme targets. Subsequently, the four most promising active compounds (quercetin, myricetrin, dihydromyricetin and taxifolin) were screened and characterized by a bioaffinity ultrafiltration method combined with LC-MS/MS analysis. Further validation of these bioactive compounds was achieved through molecular docking studies. Additionally, an interaction network was established to elucidate the mechanisms of action between the candidate constituents and their respective targets. The results indicate that A. grossedentata holds great promise as a potential natural therapeutic functional food for managing diabetes, overweight, and hyperuricemia in the foreseeable future.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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