{"title":"Antidiabetic and hypolipidemic activities of Sanghuangporus vaninii compounds in streptozotocin-induced diabetic mice via modulation of intestinal microbiota","authors":"Zirui Huang, Kewen Chen, Tiantian Li, Xiaoyu He, Xiaodong Ge, Xiaoyan Liu, Bin Liu, Feng Zeng","doi":"10.1002/efd2.70007","DOIUrl":"https://doi.org/10.1002/efd2.70007","url":null,"abstract":"<p><i>Sanghuangporus vaninii</i> is a traditional edible and medicinal fungi with a long history in China. Recent studies have focused on its potential in treating diabetes, although the underlying mechanism remains unclear. This study aimed to investigate the antidiabetic and hypolipidemic effects of the formula of <i>S. vaninii</i> compounds (SVC) in diabetic mice and explore the potential improvement in the intestinal microbiota. Oral administration of SVC significantly reduced fasting blood glucose and improved insulin sensitivity and lipid indexes. In addition to its direct effects on glucose levels and insulin sensitivity, SVC was found to have an impact on the intestinal microbiota. The levels of short-chain fatty acids receiving SVC intervention increased, and 16S rRNA sequencing revealed changes in the abundances of certain bacteria, such as <i>Harryflintia</i>, <i>Helicobacter</i>, <i>Candidatus_Soleaferrea</i>, <i>Klebsiella</i>, and <i>Candidatus_Stoquefichus</i>. These bacteria were found to be negatively associated with glucose and lipid indexes and positively related to body weight and insulin sensitivity. Overall, this study highlights the potential of SVC in treating diabetes and provides a novel perspective for understanding its antidiabetic mechanism through modulation of the intestinal microbiota.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142439081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Co-encapsulation of ω-3 LC-PUFAs and carotenoids for enhanced synergistic antioxidant effects","authors":"Yongchao Zhu, Chuang Zhang, Xinyi Sun, Jingyuan Wen, Siew Young Quek","doi":"10.1002/efd2.70016","DOIUrl":"https://doi.org/10.1002/efd2.70016","url":null,"abstract":"<p>This work aimed to study the co-encapsulation of <i>ω</i>-3 Long-Chain Polyunsaturated Fatty Acids (LC-PUFAs) with <i>β</i>-carotene, lutein and zeaxanthin and the cellular uptake of carotenoids. Monodisperse microcapsules with good properties, including particle surface morphology, water activity, microencapsulation efficiency, and glass transition temperature, were successfully prepared using a microfluidic-jet spray drier with OSA-modified starch as a wall matrix. The presence of carotenoids enhanced the oxidative stability of <i>ω</i>-3 LC-PUFAs. Caco-2 cell uptakes of the carotenoids from the spray-dried microcapsules were studied and compared to those encapsulated in the methylcellulose (MC) or xanthan gum (XG) delivery systems. The microcapsules encapsulated by OSA-modified starch showed higher cellular uptakes of carotenoids (i.e., <i>β</i>-carotene, 0.31 μg/mg protein; lutein, 0.52 μg/mg protein; zeaxanthin, 0.54 μg/mg protein) than those in the MC or XG delivery systems. The Caco-2 cell uptake depended on carotenoid type, where hydrocarbon carotenoids (i.e., <i>β</i>-carotene) had lower uptake than oxygenated carotenoids (i.e., lutein or zeaxanthin).</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nutritional composition, phytochemical profile, and health benefits of Hylocereus Undatus (pitaya): A comprehensive review","authors":"Ashok Kumar Bishoyi, Farhan Saeed, Umber Shehzadi, Amar Shankar, Jadhav Balaji, Jaspreet Kaur, Muhammad Afzaal, Ali Imran, Musarrat Rasheed, Bilal Hussain, Muzzamal Hussain, Aftab Ahmed, Fakhar Islam, Abdela Befa Kinki","doi":"10.1002/efd2.70017","DOIUrl":"https://doi.org/10.1002/efd2.70017","url":null,"abstract":"<p><i>Hylocereus undatus</i>, occasionally referred to as the dragon fruit or white-fleshed pitaya, is a rich in nutrients fruit appreciated because of its colorful look and health benefits. It is inexpensive in calories but packed with minerals like iron, magnesium, and calcium in addition to essential nutrients like vitamin C and B vitamins. Similarly rich in dietary fiber, particularly promotes gut wellness and improves digestion, is pitaya. It additionally contains antioxidants, such as phenolic compounds, flavonoids, and betalains, which help with scavenging free radicals and reducing the detrimental effects of oxidative stress on the human body. The substantial amount of betalains in <i>Hylocereus undatus</i> phytochemical composition is notable because it contributes to the plant's anti-inflammatory and antioxidant properties. There has been an association among these phytochemicals and a decreased likelihood of contracting long-term conditions such as diabetes, cancer, and cardiovascular disease. Omega-3 fatty acids and omega-6 fatty acids, in particular, that reduce cholesterol levels while improving blood circulation, are plentiful in the fruit's seeds and are beneficial to heart wellness. The objective of this review is to highlight the nutritional profile, phytochemical and health benefits of <i>Hylocereus undatus</i> in detail.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of inulin on preventing drunkenness and relieving acute alcoholic intoxication of mice and preparation of its hangover beverage","authors":"Honglin Lan, Xingguo Li, Yunhui Zhang, Jiahao Wan, Yanbin Wang, Jintao Cao, Zhouya Bai, Denglin Luo, Sihai Han, Chonghui Yue, Haiyan Gao","doi":"10.1002/efd2.70015","DOIUrl":"https://doi.org/10.1002/efd2.70015","url":null,"abstract":"<p>The aim of this study was to evaluate the effects of different types of inulin on acute alcoholic intoxication (AAI) in mice and prepare its hangover beverage. Basic physical and chemical properties of different types of inulin (short-chain inulin, long-chain inulin, and phosphorylated long-chain inulin) were analyzed and given by gavage at a dose of 400 mg kg<sup>−1</sup> day<sup>−1</sup> for a continuous period of 7 days through animal behavior experiments, and the inebriation percentage, mortality rate, duration of inebriation, and sobering time were recorded with the righting reflex as the judgment criterion. The results showed that, compared with the control group, the drunkenness and mortality rates of short-chain inulin decreased by 12% and 100%, respectively, and the sober time decreased by 18%, while alcohol tolerance was also improved. The best formula for a short-chain inulin hangover drink was determined to be: 0.4% granulated sugar, 0.5% citric acid, and 0.5% pectin. These suggest that short-chain inulin may have potential in preventing AAI.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nilofar, Nadire Pelin Bahadırlı, Esraa A. Elhawary, Omayma Eldahshan, Abdel Nasser Singab, Enver Saka, Carlos L. Cespedes- Acuna, Vasil Andruch, Alina Kalyniukova, Gokhan Zengin
{"title":"Exploring the chemical composition and biological effects of four Salvia hybrids: an innovative perspective on functional yields","authors":"Nilofar, Nadire Pelin Bahadırlı, Esraa A. Elhawary, Omayma Eldahshan, Abdel Nasser Singab, Enver Saka, Carlos L. Cespedes- Acuna, Vasil Andruch, Alina Kalyniukova, Gokhan Zengin","doi":"10.1002/efd2.70012","DOIUrl":"https://doi.org/10.1002/efd2.70012","url":null,"abstract":"<p>Genus <i>Salvia</i> is considered one of the largest genera of family Lamiaceae with many recorded species and hybrids of valuable biological importance. This study involved the phytochemical characterization of four <i>Salvia</i> hybrids (KNM23 (<i>S. fructicosa</i> × <i>S. officinalis</i>), KNM101 (<i>S. aramiensis</i> × <i>S. officinalis</i>), KNM5 (<i>S. aramiensis</i> × <i>S. officinalis)</i> and KNM12 (<i>S. fructicosa</i> × <i>S. officinalis</i>)) through measuring their total phenolic content (TPC) and total flavonoid content (TFC) together with profiling through UPLC/MS<sup>n</sup> analysis followed by investigation of their antioxidant activities and enzyme inhibitory potential through different techniques. Hybrid KNM23 presented significantly high concentration of both phenolic and flavonoid, displaying a TPC of 92.10 mg GAE/g and a TFC of 50.85 mg RE/g. The UPLC/MS<sup>n</sup> profiling resulted in the tentative identification of one hundred eighty components from different phytochemical classes mainly flavonoids, phenolic acids, phenyl propanoids, tannins and many others. The antioxidant assay showed that hybrid KNM23, a hybrid of <i>S</i>. <i>fruticosa</i> and <i>S</i>. <i>officinalis</i>, exhibited the highest antioxidant activity in the DPPH, ABTS, and PBD assays, measuring 436.38 mmol TE/g, 543.65 mmol TE/g, and 3.20 mmol TE/g, respectively. KNM23 and KNM12 showed no inhibition of AChE and <i>α</i>-glucosidase. However, KMN12 demonstrated highest BChE and tyrosinase 2.57 mg GALAE/g and 12.91 mg KAE/g, respectively. Our results suggest that the <i>Salvia</i> hybrids can be considered as natural sources of bioactive compounds in the development of health-promoting applications.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohidul Islam, Fowzul Islam Fahad, Ahasanul Karim, Arifa Sultana, A. T. M. Mostafa Kamal, Md. Sohel Rana, Mohammad Nazmul Islam
{"title":"Exploring the multifaceted therapeutic potentials of Brassaiopsis palmata (leaves) through in-vitro and in-vivo approaches","authors":"Mohidul Islam, Fowzul Islam Fahad, Ahasanul Karim, Arifa Sultana, A. T. M. Mostafa Kamal, Md. Sohel Rana, Mohammad Nazmul Islam","doi":"10.1002/efd2.70009","DOIUrl":"https://doi.org/10.1002/efd2.70009","url":null,"abstract":"<p><i>Brassaiopsis palmata</i>, an androgynous tree from Araliaceae family, has been widely found in the Asian subcontinent, and reported to have potentiality against skin infection, and many therapeutic properties still unidentified. Here, our current investigation aims to discover the innovative pharmacological potentials of the methanol extract of <i>B. palmata</i> leaves (MEBPL) through in-vivo and in-vitro approaches. Several secondary metabolites were revealed throughout the qualitative phytochemical screening of the plant extracts. MEBPL exhibited strong radical scavenging properties (IC<sub>50</sub> 178.13 µg/mL) through the 2, 2-diphenyl-1-picryhydrazyl (DPPH) assay, and through the quantitative (phenolic and flavonoid) assays with a moderate (LD<sub>50</sub> 153.14 µg/mL) toxic effect. In anti-inflammatory screening, MEBPL showed significant dose dependent inhibitory activity; and the peak inhibition were found 78.01 ± 1.22% and 82.46 ± 1.52% at 1000 µg/mL concentration on hypotonic-induce RBC hemolysis & protein denaturation test respectively. Moreover, the plant extracts manifested moderate percentage of clot lysis (28.24 ± 2.50%) on the investigation of thrombolytic assay. The anti-nociceptive activity of MEBPL was analyzed through acetic acid and formalin induce pain tests. Both 200 and 400 mg/kg dose of MEBPL exerted significant (<i>p </i>˂ 0.001) dose depending depletion of acetic acid induced writhing and formalin stimulated licking test which indicated strong analgesic properties of plant extracts. In addition, the outcomes of anti-depressant evaluation suggested that treatment with both 200 and 400 mg/kg doses showed potential dose depending activity on both FST and TST model. Furthermore, the plant extracts manifested dose dependent reduction of anxiety like behaviors in the rodent model. Particularly, mice administrated with 400 mg/kg dose of MEBPL significantly (<i>p</i> ˂ 0.05) enhanced the percentage of entries and time spent in the open arm in EPM, and also showed the highest amount of head dipping tendency in HBT. In contrast, the outcomes of this research suggest that <i>B. palmata</i> could be another source of antioxidant, cytotoxic, anti-inflammatory, thrombolytic, anti-nociceptive, anti-depressant, and anxiolytic agents. Further research on the mechanisms underlying bioactivities is required.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jin Dai, Zhiwei Zhou, Liangwei Chen, Shujuan Cao, Ke Luo, Jinmei Zhang, András Dinnyés, Dan Wang, Qun Sun
{"title":"A novel arabinogalactan extracted from Epiphyllum oxypetalum (DC.) Haw improves the immunity and gut microbiota in cyclophosphamide-induced immunosuppressed mice","authors":"Jin Dai, Zhiwei Zhou, Liangwei Chen, Shujuan Cao, Ke Luo, Jinmei Zhang, András Dinnyés, Dan Wang, Qun Sun","doi":"10.1002/efd2.70002","DOIUrl":"https://doi.org/10.1002/efd2.70002","url":null,"abstract":"<p>A novel type I arabinogalactan (AG-I) polysaccharide (EPS) from <i>Epiphyllum oxypetalum (DC.) Haw</i>'s flowers is hypothesized to possess immunomodulatory activity. This study investigated EPS's effects on immune functions and its potential mechanism for enhancing intestinal health in immunosuppressed mice. The results showed that supplementing EPS significantly alleviated immune organ damage, increased the thymus index (<i>p</i> < 0.01), and regulated the key immune factors, including the tumor necrosis factor-alpha (TNF-α), immunoglobulin A (IgA), and complement 3 (C3) in the liver (<i>p</i> < 0.05). EPS promoted the expression of intestinal immune barrier and chemical barrier proteins such as interferon-γ (IFN-γ) and mucin 2 (MUC2) (<i>p</i> < 0.05), effectively repairing intestinal damage. EPS improved the diversity and structure of intestinal microbiota in immunosuppressed mice (<i>p</i> < 0.05) and significantly altered the abundance of intestinal immune-related microbial taxa, including <i>Lactobacillaceae</i> and <i>Lactobacillus</i> (<i>p</i> < 0.01). Furthermore, EPS supplementation altered intestinal lactic acid metabolism, significantly increasing lactic acid levels by up to 3.4-fold (<i>p</i> < 0.01), and enhanced the expression of Gpr81, Wnt3a, and β-catenin proteins at the bottom of the colonic crypts, which may repair the intestinal physical barrier. Overall, EPS represents a novel AG-I immunomodulatory dietary polysaccharide that enhances immunity and improves gut health.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142404412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chune Liu, Aiqi Lin, Zixuan Li, Zheng Ma, Feng Liu
{"title":"Effects of live transport methods on the survivability, physiological responses, and flesh quality of turbot (Scophthalmus maximus)","authors":"Chune Liu, Aiqi Lin, Zixuan Li, Zheng Ma, Feng Liu","doi":"10.1002/efd2.70011","DOIUrl":"https://doi.org/10.1002/efd2.70011","url":null,"abstract":"<p>Waterless transport is an alternative method for live aquatic products. However, the changes that occur in the fish body after transportation are not known. Herein, the effects of different transport methods on quality of turbot (<i>Scophthalmus maximus</i>) were investigated. Fish were divided into four groups: Group A (water, 17°C), Group B (waterless, 3°C), Group C (water, 3°C), and Group D (control, 17°C). In the case of 100% survival, the maximum transport time was 40 h for Group A, 55 h for Group B, and 90 h for Group C. Both crude protein and crude fat decreased significantly (<i>p</i> < 0.05) in the treatment groups compared with the control group. Compared with control group, lactic dehydrogenase (LDH), lactic acid (LA), and serum cortisol (COR) increased significantly (<i>p</i> < 0.05) in all treatment groups. After fish recovered, LDH, LA, and COR values were all significantly (<i>p</i> < 0.05) lower than their corresponding non-recovered group. Compared with the control group, the expression of proteins related to muscle motility and stress capacity both decreased in the low-temperature, waterless group. Together, obvious damage may occur to flesh quality, liver, and heart of turbot when the transport time is >40 h, regardless of the transport method.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A review on daidzein as food supplement: Exploring its phytopharmacological and preclinical status","authors":"Sana Ahmad, Farogh Ahsan, Javed Akhtar Ansari, Tarique Mahmood, Arshiya Shamim, Shahzadi Bano, Reshu Tiwari, Vaseem Ahamad Ansari, Shafiurrahman, Mithilesh Kesari","doi":"10.1002/efd2.70008","DOIUrl":"https://doi.org/10.1002/efd2.70008","url":null,"abstract":"<p>A natural product is a compound or substance originating from a living organism and found in nature. Daidzein belongs to the class of bioflavonoids, which are plant-derived compounds with various biological activities. Predominantly exists in soybeans and several legumes in either glycoside or aglycone forms. Its primary chemical constituents include metabolites like equol and trihydroxy isoflavone, generated through the influence of intestinal bacteria. Daidzein has exhibited pharmacological impacts on different ailments, including cardiovascular disease, cancer, diabetes, skin disorders, osteoporosis, and neurodegenerative disorders. Daidzein's mechanisms of action involve interactions with estrogen receptors, as well as its antioxidant and anti-inflammatory properties, along with its ability to regulate apoptosis and the cell cycle. In efforts to enhance its solubility, stability, bioavailability, and targeting, daidzein has been innovatively formulated into novel dosage forms, including nanoparticles, liposomes, microemulsions, and nanosuspensions. As a promising nutraceutical, daidzein presents multiple health benefits and holds potential for various clinical applications. Additional investigation is required to comprehend the molecular mechanisms of this phenomenon and assess its safety. The purpose of this review is to provide a short description of the therapeutic properties, chemical composition, traditional use, toxicology profile, new insights on the dosage form, and future prospects of daidzein.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation of fat-free mulberry ice cream by using inulin and whey protein isolate as a fat substitute","authors":"Anantita Sangsuriyawong, Pathompol Suwanmongkol, Napassorn Peasura, Nichapha Kornsakkaya, Sasapin Disnil, Pornrat Sinchaipanit, Nilesh Nirmal","doi":"10.1002/efd2.70006","DOIUrl":"https://doi.org/10.1002/efd2.70006","url":null,"abstract":"<p>The standard ice cream consists of high calories and fat which could cause metabolic syndrome upon regular consumption. Therefore, this study developed a fat-free ice cream using fat replacer including inulin (carbohydrate-based) and whey protein isolate (WPI, protein-based) at various concentrations (6%, 8%, and 10%) or their combination (1:1). Additionally, freeze-dried mulberry fruit powder was used as a functional ingredient. The results indicated that both, inulin and WPI, function differently thereby affecting the physicochemical properties of the ice cream. The combination of inulin:WPI at a 4:4 ratio produced high-quality standard ice cream with a higher sensory score. Further incorporation of freeze-dried mulberry powder (2%) in a selected fat-free ice-cream base showed enhanced sensory, nutraceutical, and textural properties of the ice cream. All fat-free and fat-free mulberry ice cream had a trace amount of fat (0.06%), while the calorie was reduced to 88.9 kcal/serving compared with a full-fat ice cream (195.7 kcal/serving). Hence, fat-free mulberry ice cream with 74.5% of nonfat milk, 5.0% of skim milk powder, 8% of inulin:WPI (4:4), 9.5% of sugar, 0.5% of emulsifier, and 2% freeze-dried mulberry powder could serve as the best alternative to the full-fat ice cream.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":null,"pages":null},"PeriodicalIF":4.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}