IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70142

Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu

{"title":"Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways","authors":"Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu","doi":"10.1002/efd2.70142","DOIUrl":"https://doi.org/10.1002/efd2.70142","url":null,"abstract":"Oral cancer represents a significant global health burden owing to the limitations of current therapeutic approaches, including adverse effects and drug resistance. Phytochemicals have gained increasing attention as promising anticancer agents with multi-target effects and low toxicity. This study evaluated the anticancer potential of Phyllanthus emblica water extract (PEWE) using in vitro and in vivo models. In vitro, PEWE exhibited dose-dependent inhibition of DOK and OECM-1 oral cancer cell proliferation while sparing non-cancerous CGHNK2 cells. PEWE significantly suppressed cell migration and invasion, induced S-phase cell cycle arrest, and promoted apoptosis associated with mitochondrial dysfunction. Morphological and molecular analyses confirmed the induction of autophagy as evidenced by increased LC3 expression, decreased p62 levels, and upregulation of Rab7. RNA sequencing revealed significant modulation of key autophagy-related genes, including ITPR1, AMBRA1, and MAP1LC3B. In vivo, PEWE effectively inhibited tumor growth in xenograft and carcinogen-induced mouse oral cancer models without any apparent systemic toxicity. These findings demonstrate that PEWE exerts anticancer effects through a coordinated mechanism involving cell cycle disruption, apoptosis, and autophagy, highlighting its potential as a novel and less toxic therapeutic agent for oral cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets 用三种酶靶点的亲和超滤技术研究了长尾蛇葡萄中潜在的降血糖、降血脂和降尿酸的生物活性成分

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70144

Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo

{"title":"Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets","authors":"Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo","doi":"10.1002/efd2.70144","DOIUrl":"https://doi.org/10.1002/efd2.70144","url":null,"abstract":"Hyperuricemia demonstrates synergistic interactions in the progression of hyperlipidemia and hyperglycemia. There is a pressing demand to explore natural products with negligible adverse effects for the prevention and regulation of hyperuricemia, particularly when combined with hyperglycemia and hyperlipidemia. Ampelopsis grossedentata has been traditionally employed as a functional food throughout an extensive historical duration. Nevertheless, the bioactive compounds that contribute to its hypoglycemic, hypolipidemic, and hypouricemic effects, as well as the molecular mechanisms involved, have not yet been completely clarified. This study initially conducted in vitro assays to evaluate the inhibitory activity of A. grossedentata towards α-glucosidase, pancreatic lipase, and xanthine oxidase. The results revealed that the A. grossedentata extract exhibited substantial inhibitory activities against these enzyme targets. Subsequently, the four most promising active compounds (quercetin, myricetrin, dihydromyricetin and taxifolin) were screened and characterized by a bioaffinity ultrafiltration method combined with LC-MS/MS analysis. Further validation of these bioactive compounds was achieved through molecular docking studies. Additionally, an interaction network was established to elucidate the mechanisms of action between the candidate constituents and their respective targets. The results indicate that A. grossedentata holds great promise as a potential natural therapeutic functional food for managing diabetes, overweight, and hyperuricemia in the foreseeable future.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways 余甘子通过多种途径发挥口腔癌的治疗潜力

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70142

Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu

{"title":"Phyllanthus emblica Exerts Therapeutic Potential in Oral Cancer Through Multiple Pathways","authors":"Tai-Pei Lin, Min-Hsiung Pan, Chan-Hua Chang, Kuan-Chung Hsiao, Yen-Lin Chen, Jui-Sheng Lai, Wen-Chan Yang, Ann-Joy Cheng, Yin-Ju Chen, Chiou-Ying Yang, Meng-Yuan Huang, Ko-Jiunn Liu","doi":"10.1002/efd2.70142","DOIUrl":"https://doi.org/10.1002/efd2.70142","url":null,"abstract":"Oral cancer represents a significant global health burden owing to the limitations of current therapeutic approaches, including adverse effects and drug resistance. Phytochemicals have gained increasing attention as promising anticancer agents with multi-target effects and low toxicity. This study evaluated the anticancer potential of Phyllanthus emblica water extract (PEWE) using in vitro and in vivo models. In vitro, PEWE exhibited dose-dependent inhibition of DOK and OECM-1 oral cancer cell proliferation while sparing non-cancerous CGHNK2 cells. PEWE significantly suppressed cell migration and invasion, induced S-phase cell cycle arrest, and promoted apoptosis associated with mitochondrial dysfunction. Morphological and molecular analyses confirmed the induction of autophagy as evidenced by increased LC3 expression, decreased p62 levels, and upregulation of Rab7. RNA sequencing revealed significant modulation of key autophagy-related genes, including ITPR1, AMBRA1, and MAP1LC3B. In vivo, PEWE effectively inhibited tumor growth in xenograft and carcinogen-induced mouse oral cancer models without any apparent systemic toxicity. These findings demonstrate that PEWE exerts anticancer effects through a coordinated mechanism involving cell cycle disruption, apoptosis, and autophagy, highlighting its potential as a novel and less toxic therapeutic agent for oral cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets 用三种酶靶点的亲和超滤技术研究了长尾蛇葡萄中潜在的降血糖、降血脂和降尿酸的生物活性成分

IF 5.7

eFood Pub Date : 2026-03-19 DOI: 10.1002/efd2.70144

Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo

{"title":"Potential Hypoglycemic, Hypolipidemic, and Hypouricemic Bioactive Components From Ampelopsis grossedentata Explored by Affinity Ultrafiltration With Three Enzyme Targets","authors":"Siyang Mao, Chu Chu, Yanzhi Xiong, Mengjia Xu, Jian Yang, Mingquan Guo","doi":"10.1002/efd2.70144","DOIUrl":"https://doi.org/10.1002/efd2.70144","url":null,"abstract":"Hyperuricemia demonstrates synergistic interactions in the progression of hyperlipidemia and hyperglycemia. There is a pressing demand to explore natural products with negligible adverse effects for the prevention and regulation of hyperuricemia, particularly when combined with hyperglycemia and hyperlipidemia. Ampelopsis grossedentata has been traditionally employed as a functional food throughout an extensive historical duration. Nevertheless, the bioactive compounds that contribute to its hypoglycemic, hypolipidemic, and hypouricemic effects, as well as the molecular mechanisms involved, have not yet been completely clarified. This study initially conducted in vitro assays to evaluate the inhibitory activity of A. grossedentata towards α-glucosidase, pancreatic lipase, and xanthine oxidase. The results revealed that the A. grossedentata extract exhibited substantial inhibitory activities against these enzyme targets. Subsequently, the four most promising active compounds (quercetin, myricetrin, dihydromyricetin and taxifolin) were screened and characterized by a bioaffinity ultrafiltration method combined with LC-MS/MS analysis. Further validation of these bioactive compounds was achieved through molecular docking studies. Additionally, an interaction network was established to elucidate the mechanisms of action between the candidate constituents and their respective targets. The results indicate that A. grossedentata holds great promise as a potential natural therapeutic functional food for managing diabetes, overweight, and hyperuricemia in the foreseeable future.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147567176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Different Traditional and Modern Coffee Brewing Methods Affect Its Aroma, Chemistry, Health Effects and Safety Attributes?—A Comprehensive Review to Maximize Coffee Brew Quality 不同的传统和现代咖啡冲泡方法对其香气、化学成分、健康效果和安全属性的影响？-全面审查，最大限度地提高咖啡冲泡质量

IF 5.7

eFood Pub Date : 2026-03-16 DOI: 10.1002/efd2.70137

Enas A. El-Hawary, Hania A. Guirguis, Nik Muhammad Azhar Nik Daud, Mohd Asraf Zainudin, Ahmed Mediani, Mohamed A. Farag

{"title":"How Different Traditional and Modern Coffee Brewing Methods Affect Its Aroma, Chemistry, Health Effects and Safety Attributes?—A Comprehensive Review to Maximize Coffee Brew Quality","authors":"Enas A. El-Hawary, Hania A. Guirguis, Nik Muhammad Azhar Nik Daud, Mohd Asraf Zainudin, Ahmed Mediani, Mohamed A. Farag","doi":"10.1002/efd2.70137","DOIUrl":"10.1002/efd2.70137","url":null,"abstract":"Coffee is a primary beverage that is consumed worldwide as a chief beverage. In addition to its taste and functionality as an “energizer,” it encompasses a myriad of bioactive compounds that exert several health benefits. The coffee brewing process is an intricate process during which multiple factors shape the chemical and sensory profile of the final brew. This review provides a holistic overview of traditional and modern coffee pre-brewing methods, while emphasizing the methodology and main parameters involved in each brewing technique. The study summarizes how different brewing methods can impact coffee metabolome, antioxidant, sensory attributes, and health benefits to identify the best method regarding each attribute. A comparison of the physicochemical characteristics, including density, particle size, viscosity, aroma, and acidity in the different coffee beverages, is presented for consumer preferences. Traditional brewing methods of coffee such as decoction tend to emphasize the aroma, while infusion offers more enhanced flavor due to higher levels of caffeoylquinic acids. In addition, modern pressurized methods such as Espresso machines yielded more concentrated beverages with the highest caffeine content (60 mg/50 mL). From a health perspective, filtered coffee is preferable due to the removal of diterpenes, which increase the risk for hypercholesterolemia and hypertension.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147566477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Heat Treatment on the Flavor Characteristics of Vacuum Freeze-Dried Pacific White Shrimp (Litopenaeus vannamei) 热处理对真空冻干凡纳滨对虾风味特性的影响

IF 5.7

eFood Pub Date : 2026-03-08 DOI: 10.1002/efd2.70135

Zhongxu Guo, Zhankai Zhang, Yuhao Wu, Xiaodan Fu, Zhenlin Han, Chao Liu, Ningyang Li

{"title":"Effect of Heat Treatment on the Flavor Characteristics of Vacuum Freeze-Dried Pacific White Shrimp (Litopenaeus vannamei)","authors":"Zhongxu Guo, Zhankai Zhang, Yuhao Wu, Xiaodan Fu, Zhenlin Han, Chao Liu, Ningyang Li","doi":"10.1002/efd2.70135","DOIUrl":"https://doi.org/10.1002/efd2.70135","url":null,"abstract":"Vacuum freeze-drying (VFD) effectively preserves the texture of Litopenaeus vannamei but often results in a bland flavor profile. To address this, this study investigated a Post-Vacuum-Freeze-Drying Heat Treatment (HAFD) strategy, profiling flavor evolution via E-Tongue, E-Nose, and GC-MS. Results demonstrated that HAFD optimized biochemical palatability; total free amino acids increased by ~12%, and the equivalent umami concentration (EUC) peaked at 51.41 g MSG/100 g, driven by synergistic interactions between nucleotides and amino acids. Volatile analysis revealed that 150°C treatments triggered significant Maillard reactions, causing an explosive generation of nitrogen-containing heterocycles. Notably, 2,3,5-trimethylpyrazine surged from 0.76 to 52.15 ng/g, imparting critical “roasted” attributes while retaining fresh seafood notes like 1-octen-3-ol. Conclusively, baking at 150°C for 2–5 min is identified as the optimal parameter to balance premium texture with superior, appetizing flavor. This finding provides a theoretical basis and technical guidance for developing high-value, ready-to-eat shrimp products that combine the textural benefits of freeze-drying with the appetizing aroma of roasting.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147564218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Heat Treatment on the Flavor Characteristics of Vacuum Freeze-Dried Pacific White Shrimp (Litopenaeus vannamei) 热处理对真空冻干凡纳滨对虾风味特性的影响

IF 5.7

eFood Pub Date : 2026-03-08 DOI: 10.1002/efd2.70135

Zhongxu Guo, Zhankai Zhang, Yuhao Wu, Xiaodan Fu, Zhenlin Han, Chao Liu, Ningyang Li

{"title":"Effect of Heat Treatment on the Flavor Characteristics of Vacuum Freeze-Dried Pacific White Shrimp (Litopenaeus vannamei)","authors":"Zhongxu Guo, Zhankai Zhang, Yuhao Wu, Xiaodan Fu, Zhenlin Han, Chao Liu, Ningyang Li","doi":"10.1002/efd2.70135","DOIUrl":"https://doi.org/10.1002/efd2.70135","url":null,"abstract":"Vacuum freeze-drying (VFD) effectively preserves the texture of Litopenaeus vannamei but often results in a bland flavor profile. To address this, this study investigated a Post-Vacuum-Freeze-Drying Heat Treatment (HAFD) strategy, profiling flavor evolution via E-Tongue, E-Nose, and GC-MS. Results demonstrated that HAFD optimized biochemical palatability; total free amino acids increased by ~12%, and the equivalent umami concentration (EUC) peaked at 51.41 g MSG/100 g, driven by synergistic interactions between nucleotides and amino acids. Volatile analysis revealed that 150°C treatments triggered significant Maillard reactions, causing an explosive generation of nitrogen-containing heterocycles. Notably, 2,3,5-trimethylpyrazine surged from 0.76 to 52.15 ng/g, imparting critical “roasted” attributes while retaining fresh seafood notes like 1-octen-3-ol. Conclusively, baking at 150°C for 2–5 min is identified as the optimal parameter to balance premium texture with superior, appetizing flavor. This finding provides a theoretical basis and technical guidance for developing high-value, ready-to-eat shrimp products that combine the textural benefits of freeze-drying with the appetizing aroma of roasting.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147564217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Capsazepine as a Novel MCL1 Inhibitor for Overcoming Tamoxifen Resistance in Breast Cancer Therapy Capsazepine作为一种新的MCL1抑制剂在乳腺癌治疗中克服他莫昔芬耐药性的发现

IF 5.7

eFood Pub Date : 2026-03-01 DOI: 10.1002/efd2.70136

Shujing Liu, Jiayin Li, Chengkun Wu, Yupeng Xiao, Jing Yu, Yu Xie, Chen Lin, Chunhua Liu, Yue Wang, Junfang Qin

{"title":"Discovery of Capsazepine as a Novel MCL1 Inhibitor for Overcoming Tamoxifen Resistance in Breast Cancer Therapy","authors":"Shujing Liu, Jiayin Li, Chengkun Wu, Yupeng Xiao, Jing Yu, Yu Xie, Chen Lin, Chunhua Liu, Yue Wang, Junfang Qin","doi":"10.1002/efd2.70136","DOIUrl":"10.1002/efd2.70136","url":null,"abstract":"Tamoxifen resistance remains a significant clinical challenge in breast cancer. The anti-apoptotic protein MCL1 is frequently overexpressed in tamoxifen-resistant tumors and is associated with poor patient survival, underscoring its potential as a therapeutic target. To identify novel MCL1 inhibitors, we first confirmed MCL1 upregulation in tamoxifen-resistant MCF7-R cells and clinical specimens. Using high-throughput virtual screening, molecular docking, and dynamics simulations, we identified capsazepine (CPZ) as a promising candidate. CPZ exhibited high binding affinity and formed stable interactions with key MCL1 residues, LEU267 and PHE270. Experimentally, CPZ significantly suppressed proliferation and induced mitochondrial-dependent apoptosis in MCF7-R cells. Importantly, CPZ synergized with tamoxifen to suppress colony formation and promote apoptosis, effectively reversing the resistant phenotype. Mechanistic studies using Drug Affinity Responsive Target Stability (DARTS) and functional assays confirmed that CPZ directly binds to and stabilizes MCL1. CPZ also demonstrated favorable drug-like properties and a superior predicted toxicity profile compared to the established inhibitor UMI-77. Our findings establish CPZ as a novel and effective MCL1 inhibitor that can help overcome tamoxifen resistance, offering a promising combined therapeutic strategy for endocrine-resistant breast cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Capsazepine as a Novel MCL1 Inhibitor for Overcoming Tamoxifen Resistance in Breast Cancer Therapy Capsazepine作为一种新的MCL1抑制剂在乳腺癌治疗中克服他莫昔芬耐药性的发现

IF 5.7

eFood Pub Date : 2026-03-01 DOI: 10.1002/efd2.70136

Shujing Liu, Jiayin Li, Chengkun Wu, Yupeng Xiao, Jing Yu, Yu Xie, Chen Lin, Chunhua Liu, Yue Wang, Junfang Qin

{"title":"Discovery of Capsazepine as a Novel MCL1 Inhibitor for Overcoming Tamoxifen Resistance in Breast Cancer Therapy","authors":"Shujing Liu, Jiayin Li, Chengkun Wu, Yupeng Xiao, Jing Yu, Yu Xie, Chen Lin, Chunhua Liu, Yue Wang, Junfang Qin","doi":"10.1002/efd2.70136","DOIUrl":"https://doi.org/10.1002/efd2.70136","url":null,"abstract":"Tamoxifen resistance remains a significant clinical challenge in breast cancer. The anti-apoptotic protein MCL1 is frequently overexpressed in tamoxifen-resistant tumors and is associated with poor patient survival, underscoring its potential as a therapeutic target. To identify novel MCL1 inhibitors, we first confirmed MCL1 upregulation in tamoxifen-resistant MCF7-R cells and clinical specimens. Using high-throughput virtual screening, molecular docking, and dynamics simulations, we identified capsazepine (CPZ) as a promising candidate. CPZ exhibited high binding affinity and formed stable interactions with key MCL1 residues, LEU267 and PHE270. Experimentally, CPZ significantly suppressed proliferation and induced mitochondrial-dependent apoptosis in MCF7-R cells. Importantly, CPZ synergized with tamoxifen to suppress colony formation and promote apoptosis, effectively reversing the resistant phenotype. Mechanistic studies using Drug Affinity Responsive Target Stability (DARTS) and functional assays confirmed that CPZ directly binds to and stabilizes MCL1. CPZ also demonstrated favorable drug-like properties and a superior predicted toxicity profile compared to the established inhibitor UMI-77. Our findings establish CPZ as a novel and effective MCL1 inhibitor that can help overcome tamoxifen resistance, offering a promising combined therapeutic strategy for endocrine-resistant breast cancer.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Mechanism of Auricularia auricula Polysaccharides Against Acute Alcoholic Liver Injury Is Related to Different Consumption Doses 黑木耳多糖对急性酒精性肝损伤的保护机制与不同剂量有关

IF 5.7

eFood Pub Date : 2026-02-28 DOI: 10.1002/efd2.70125

Haili Niu, Fangjia Zhao, Zeyu Hu, Qi Wang, Yuan Li, Ivan Kurtovic, Yahong Yuan, Tianli Yue

{"title":"Protective Mechanism of Auricularia auricula Polysaccharides Against Acute Alcoholic Liver Injury Is Related to Different Consumption Doses","authors":"Haili Niu, Fangjia Zhao, Zeyu Hu, Qi Wang, Yuan Li, Ivan Kurtovic, Yahong Yuan, Tianli Yue","doi":"10.1002/efd2.70125","DOIUrl":"10.1002/efd2.70125","url":null,"abstract":"This study investigated the dose-dependent protective effects of Auricularia auricula polysaccharides (AAP) against acute alcoholic liver injury (AALI) via the gut-liver axis in mice. Results indicated that low-, medium-, and high-dose (100, 200, and 400 mg/kg) AAP conferred protection through distinct mechanisms. The low dose alleviated hepatic steatosis by reducing hepatic triglyceride content (0.041 mmol/g prot vs. EtOH group 0.094 mmol/g prot). The medium dose demonstrated potent antioxidant activity, enhancing hepatic catalase (7.02 U/mg prot vs. EtOH 3.28 U/mg prot), superoxide dismutase (107.70 U/mg prot vs. EtOH 81.07 U/mg prot), and glutathione peroxidase (30.08 U/mg prot vs. EtOH 21.99 U/mg prot), while reducing malondialdehyde levels (0.135 nmol/mg prot vs. EtOH 0.309 nmol/mg prot). The high dose excelled at restoring liver function, lowering serum alanine aminotransferase (10.10 U/L vs. EtOH 29.86 U/L) and aspartate aminotransferase activity (24.85 U/L vs. EtOH 35.62 U/L), and exerted anti-inflammatory effects by suppressing TNF-α (459.9 pg/mL vs. EtOH 716.7 pg/mL), IL-1β (333.4 pg/mL vs. EtOH 621.4 pg/mL), and IL-6 (83.3 pg/mL vs. EtOH 162.9 pg/mL). All AAP treatments elevated characteristic beneficial bacteria including Bacteroides, Muribaculum, and Parabacteroides. These findings demonstrate AAP protects against AALI through coordinated, dose-specific mechanisms that ameliorate hepatic damage and reshape intestinal flora.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147570188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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