IF 5.7

eFood Pub Date : 2025-08-12 DOI: 10.1002/efd2.70089

Amal Sudaraka Samarasinghe, Fabiola Fernando, Kerthika Devi Athiyappan, Baojun Xu, Abu Saeid

{"title":"New Insights Into the Use of Cereals and Pseudocereals in Fermented Beverages: Trends, Challenges, and Innovations","authors":"Amal Sudaraka Samarasinghe, Fabiola Fernando, Kerthika Devi Athiyappan, Baojun Xu, Abu Saeid","doi":"10.1002/efd2.70089","DOIUrl":"10.1002/efd2.70089","url":null,"abstract":"Nowadays, cereals and pseudocereals are crucial in producing fermented drinks, conferring their nutritional, functional, and sensory properties. This review considered the transition from the traditional grains (i.e., barley, wheat, rice, and maize) to pseudocereals (i.e., buckwheat, quinoa, and amaranth) and hybrid cereals (i.e., triticale and tritordeum), induced by the demand for the gluten-free, nutritious, and sustainable foods. The aims of this review include assessment of their compositional benefits (e.g., proteins, fiber, and antioxidants), technical challenges (e.g., enzymatic limitations and process scalability), and innovations (e.g., enzyme-catalyzed processing, extrusion, and artificial intelligence-based optimization) to improve brewing efficiency and the quality of the final products. As novel grains open up the market potential and promote the health and sustainability trends, assessing the technological challenges, such as raw material heterogeneity and enzymatic flexibility, presents challenging tasks for industrially viable deployment. Such emerging strategy options can redefine brewing procedures, enable innovation, and meet consumers' demands.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Quality Differences Between Shandong Green Tea and Low-Latitude Green Tea 山东绿茶与低纬度绿茶品质差异的研究

IF 5.7

eFood Pub Date : 2025-08-08 DOI: 10.1002/efd2.70087

Dewen Wang, Chunwang Dong, Mengqi Guo, Yali Shi, Fanan Zhang, Xingmin Zhang, Min Lu

{"title":"A Study on Quality Differences Between Shandong Green Tea and Low-Latitude Green Tea","authors":"Dewen Wang, Chunwang Dong, Mengqi Guo, Yali Shi, Fanan Zhang, Xingmin Zhang, Min Lu","doi":"10.1002/efd2.70087","DOIUrl":"10.1002/efd2.70087","url":null,"abstract":"Shandong, the tea-growing region at the highest latitude in China, leverages its unique climatic environment and geographical advantages to produce numerous high-quality green teas, including Rizhao Green Tea. Thus, By investigating the differences in leaf characteristics and physicochemical aspects between green teas from Shandong and those grown at varying altitudes in low-latitude regions of southern China, this study aimed to uncover the unique qualities of Shandong green teas. Correlations between leaf characteristics, sensory quality, and physicochemical components in tea samples from the four provinces were analyzed, elucidating the intricate relationships among these three indicators. Through PLS-DA and significance difference analysis of 34 physicochemical components, 14 significantly different components were selected on the basis of the criteria of VIP > 1 and p < 0.05. Notably, four major components—water extract, free amino acids, caffeine, and total polyphenols—were found to be abundant in the Shandong samples, while the phenol-to-ammonia ratio was ideally balanced between 4.5 and 5.5, contributing to the highest sensory evaluation scores. The findings of this study provide scientific insights for tea production, facilitating the optimization and upgrading of the tea industry and enhancing its market competitiveness.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145128953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics, Bioactives and Antioxidant Activity of Illawarra Plum (Podocarpus elatus) Fruit 红梅果实特性、生物活性及抗氧化活性研究

IF 5.7

eFood Pub Date : 2025-07-31 DOI: 10.1002/efd2.70088

Rebecca McCullum, Md Saifullah, Michael Bowyer, Quan V. Vuong

{"title":"Characteristics, Bioactives and Antioxidant Activity of Illawarra Plum (Podocarpus elatus) Fruit","authors":"Rebecca McCullum, Md Saifullah, Michael Bowyer, Quan V. Vuong","doi":"10.1002/efd2.70088","DOIUrl":"10.1002/efd2.70088","url":null,"abstract":"Illawarra plums (IP) are native to Australia, having been used as bush food for centuries. This study characterized the physicochemical and antioxidant properties of mature IP and explored the efficacy of aqueous ethanol for extracting bioactives. The height, width, diameter, and weight of the fruit are 14.78 ± 3.1 mm, 17.45 ± 2.7 mm, 14.69 ± 3.0 mm, and 2.59 ± 1.2 g, respectively. The fruits were categorized into three ripening stages: unripe(green), almost-ripe(blushing), and ripe(red). The ripe fruit had a pH of 4.37 ± 0.03, total soluble solids of 9.3 ± 0.5°Brix, and a titratable acidity of 0.25 ± 0.01% w/v. The extraction solvents significantly influenced the yield of bioactives and antioxidant activity. The most effective solvent was 50% ethanol, which had total phenolics 123.93 ± 10.81 mg, flavonoids 130.58 ± 23.33 mg, proanthocyanins, and anthocyanins 16.12 ± 0.69 mg per gram of dried fruit. The extract exhibited potent DPPH radical scavenging properties (153.22 ± 39.67 mg TE/g). IP had five times more phenolics than African and American plums. Fourteen peaks were isolated by HPLC-PDA, with three tentatively identified as chlorogenic acid, epicatechin, and p-coumaric acid. IP shows great potential for the development of natural functional ingredients. Future research could explore individual phenolics and investigate the potential applications of IP in the food industry.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Characterization and Physicochemical Property of a Purified Fucosylated Glycosaminoglycan Sulfate From Holothuria leucospilota 一种纯化的白花海棠浓缩硫酸糖胺聚糖的结构表征和理化性质

IF 5.7

eFood Pub Date : 2025-07-29 DOI: 10.1002/efd2.70071

Wanting Wang, Kexue Zhu, Jun Cao, Shunjiang Zeng, Lijun You, Chao Zhao, Yuanping Zheng, Chuan Li

{"title":"Structural Characterization and Physicochemical Property of a Purified Fucosylated Glycosaminoglycan Sulfate From Holothuria leucospilota","authors":"Wanting Wang, Kexue Zhu, Jun Cao, Shunjiang Zeng, Lijun You, Chao Zhao, Yuanping Zheng, Chuan Li","doi":"10.1002/efd2.70071","DOIUrl":"10.1002/efd2.70071","url":null,"abstract":"A sulfated polysaccharide was purified from the body wall of the sea cucumber Holothuria leucospilota (HLP-Ⅰ). The structure of polysaccharides is critical for understanding and controlling their functional properties. Chemical and nuclear magnetic resonance (NMR) spectroscopic methods were applied for the primary structural characterization of this biopolymer. HLP-Ⅰ was a fucosylated glycosaminoglycan sulfate (FGs) with a weight-average molecular mass of 106.2 kDa. The main monosaccharide composition of HLP-Ⅰ is d-glucuronic acid (d-GlcA), n-acetylgalactosamine (n-GalNAc), and l-fucose (l-Fuc). And the sulfation patterns of the fucose branches were Fuc0S, Fuc3,4S, and Fuc2,4S at a ratio of 0.65:1.00:0.40. Moreover, HLP-Ⅰ was thoroughly characterized using high-performance size-exclusion chromatography (HPSEC), microscopy (SEM and AFM), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). HLP-Ⅰ showed high amorphous platelets content and disordered arrangement, while the molecule adapted a sphere chain conformation in the 0.1 M NaNO3 solution. Microscopy images showed that HLP-Ⅰ was a relatively smooth thin layer of hollow spherical particles. The gelation temperature was shown to be 211.6°C by DSC.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Perspectives on the Role of Anthocyanins in Blueberries (Vaccinium spp.) Against Cardiovascular Diseases and Their Complications: An Updated Review 蓝莓花色苷抗心血管疾病及其并发症的研究进展

IF 5.7

eFood Pub Date : 2025-07-28 DOI: 10.1002/efd2.70072

Muhammad Tayyab Arshad, Sammra Maqsood, Ali Ikram, Muhammed Adem Abdullahi

{"title":"Recent Perspectives on the Role of Anthocyanins in Blueberries (Vaccinium spp.) Against Cardiovascular Diseases and Their Complications: An Updated Review","authors":"Muhammad Tayyab Arshad, Sammra Maqsood, Ali Ikram, Muhammed Adem Abdullahi","doi":"10.1002/efd2.70072","DOIUrl":"10.1002/efd2.70072","url":null,"abstract":"Blueberries (Vaccinium spp.) are renowned for their high content of anthocyanins which are bioactive molecules possessing potent anti-inflammatory and antioxidant properties. Improved dietary intervention is desperately needed as the primary cause of death globally for cardiovascular diseases (CVDs). The health benefits of blueberry anthocyanins and their mechanism of action in preventing CVDs have been critically reviewed. Key findings indicate that blueberry anthocyanins develop endothelial function by enhancing nitric oxide bioavailability, altering inflammatory pathways, and decreasing oxidative stress. Clinical trial data support their ability to diminish blood pressure, improve lipid profiles, and sluggish the progression of atherosclerosis. Mechanistic insights indicate that anthocyanins are cardioprotective agents that refine arterial health, inducing vasodilation and constraining platelet aggregation. The review also touches on the bioavailability and metabolism of anthocyanins, the factors influencing their efficacy and their potential inclusion in heart-healthy diets. Notwithstanding the promising results hurdles must be crossed to ensure optimum anthocyanin bioavailability and consistent dietary recommendations. Future studies should focus on explaining the molecular mechanisms of the health benefits of blueberry anthocyanins and long-term cardiovascular outcomes.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing Cordyceps cicadae Mycelium as a Potential Supplement for Alleviating UVB-Induced Photokeratitis Symptoms In Vivo 利用冬虫夏草菌丝体作为缓解uvb诱导的光性角膜炎症状的潜在补充剂

IF 5.7

eFood Pub Date : 2025-07-18 DOI: 10.1002/efd2.70083

Tsung-Han Lu, Szu-Yu Kuo, Jui-Hsia Hsu, Yen-Lien Chen, Han-Hsin Chang, Chin-Chu Chen, David Pei-Cheng Lin

{"title":"Utilizing Cordyceps cicadae Mycelium as a Potential Supplement for Alleviating UVB-Induced Photokeratitis Symptoms In Vivo","authors":"Tsung-Han Lu, Szu-Yu Kuo, Jui-Hsia Hsu, Yen-Lien Chen, Han-Hsin Chang, Chin-Chu Chen, David Pei-Cheng Lin","doi":"10.1002/efd2.70083","DOIUrl":"10.1002/efd2.70083","url":null,"abstract":"Ultraviolet (UV) radiation exposure to the cornea can lead to photokeratitis, disrupting tear film homeostasis and increasing the risk of dry eye disease (DED). While Cordyceps cicadae mycelium extracts (CCME) have been reported to benefit ocular diseases, their potential to alleviate UVB-induced photokeratitis remains unexplored. This study investigated the CCME's protective effects against UVB-induced corneal damage. ICR mice were randomly assigned to three groups: a control group, a UVB-exposed group with vehicle treatment (UVB group), and a UVB-exposed group treated with CCME (CCME group). Tear volume (TV), tear break-up time (TBUT), ocular surface integrity, conjunctival goblet cell density, and inflammatory markers were evaluated. The results demonstrated that CCME intake significantly improved TV and TBUT, while reducing oxidative stress and inflammatory markers (p63 + , PCNA, NF-κB, and COX-2). Notably, CCME treatment helped to preserve goblet cells and maintain Muc5Ac expression therein, with concomitant suppression on lipid peroxidation (as indicated by MDA and 4-HNE reduction) in the meibomian glands, suggesting its role in stabilizing tear film composition. This study advances the field by introducing CCME as a potential therapeutic agent for photokeratitis, offering a natural, oral treatment alternative with anti-inflammatory and antioxidative properties. CCME could help prevent progression to chronic DED and other ocular surface disorders by mitigating corneal inflammation and preserving tear film components. Given the elevated prevalence of UV-induced ocular damage due to environmental changes, these findings provide a foundation for developing CCME-based interventions in ophthalmology, bridging traditional medicine with modern therapeutic interventions.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laxative Effect of Lactobacillus paracasei Glory LP16 on Loperamide-Induced Constipation in Mice 副干酪乳杆菌LP16对洛哌丁胺致小鼠便秘的通便作用

IF 5.7

eFood Pub Date : 2025-07-14 DOI: 10.1002/efd2.70068

Weiwei Ma, Lian Lian, Lidong Guo, Yanan Wu, Lili Huang

{"title":"Laxative Effect of Lactobacillus paracasei Glory LP16 on Loperamide-Induced Constipation in Mice","authors":"Weiwei Ma, Lian Lian, Lidong Guo, Yanan Wu, Lili Huang","doi":"10.1002/efd2.70068","DOIUrl":"10.1002/efd2.70068","url":null,"abstract":"Constipation is a common gastrointestinal disease. In recent years, probiotic intervention has often been used to solve such problems. However, the results of existing studies on different intervention times and dose differences are not consistent. Therefore, it is necessary to conduct research on specific strains. In this study, loperamide was used to establish the constipation model. By observing the basic fecal parameters, gastrointestinal characteristics, histological morphology, short-chain fatty acids, and microbiota composition of constipated mice, the remission mechanisms of different doses of Lactobacillus paracasei Glory LP16 (LP16) on constipated mice were compared. It was found that different doses of LP16 could improve gastrointestinal peristalsis and neurotransmitter levels in constipated mice, reduce intestinal inflammation, significantly increase the concentration of short-chain fatty acids, and improve the intestinal flora. Macrogenomics indicates that LP16 is mainly enriched in metabolic pathways such as amino acid biosynthesis and amino acid and nucleotide sugar metabolism. The expression of glycoside hydrolase in mice increased significantly. The results show that LP16 has laxative and antidiarrheal effects. Its laxative and antidiarrheal mechanisms may be related to its effects on constipation-related neurotransmitters, improving intestinal flora, increasing fecal acetic acid content in constipated mice, and alleviating constipation-induced inflammation.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fucoidan From Brown Algae as a Functional Food Ingredient: A Promising Anticancer Agent 褐藻褐藻聚糖作为功能性食品成分：一种有前景的抗癌剂

IF 5.7

eFood Pub Date : 2025-07-14 DOI: 10.1002/efd2.70085

Muhammad Umer Khan, Iqra Khurram, Maha Munir, Muhammad Ikram Ullah, Heba Bassiony Ghanem, Javad Sharifi-Rad, William N. Setzer, Daniela Calina

{"title":"Fucoidan From Brown Algae as a Functional Food Ingredient: A Promising Anticancer Agent","authors":"Muhammad Umer Khan, Iqra Khurram, Maha Munir, Muhammad Ikram Ullah, Heba Bassiony Ghanem, Javad Sharifi-Rad, William N. Setzer, Daniela Calina","doi":"10.1002/efd2.70085","DOIUrl":"10.1002/efd2.70085","url":null,"abstract":"Fucoidan is a naturally occurring, sulfated fucose-based polymer found in many types of brown algae. Fucoidan has recently attracted interest in the field of oncology due to its biological and pharmacological features, such as antitumor, anti-proliferation, and anti-inflammatory effects. Fucoidan's attributes are mostly determined by its relative molecular mass and the processes of purification and extraction, which result in structural modifications and can alter outcomes. Several in vivo and in vitro research are being conducted to investigate fucoidan's anticancer potential so that it can be employed as a therapeutic agent; also, decreased toxicity and in vitro impacts of fucoidan make it feasible for cancer therapy. The current review will summarize various aspects of fucoidan including the source and structural activity of fucoidan along with the anticancer relationship of fucoidan on multiple cell lines. In this review, the bioavailability and pharmacological properties are also discussed along with the mechanism of action through which fucoidan inhibits proliferation or induces apoptosis in different tumors, suggesting it is a potential therapeutic agent for combating various tumors.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rosa roxburghii Tratt Fruit Juice Ameliorates DSS-Induced Colitis by Improving Intestinal Barrier and Gut Microbiota Independently of Vitamin C 刺梨果汁通过改善肠道屏障和独立于维生素C的肠道微生物群来改善dss诱导的结肠炎

IF 5.7

eFood Pub Date : 2025-07-14 DOI: 10.1002/efd2.70084

Wenyue Chen, Dafeng Fang, Lili Zhang, Mingqing Kuang, Mengyao Zhang, Chenxiao Shen, Yi Liu, Hefeng Zhou, Shengpeng Wang, Wenwen Zhao, Yitao Wang, Zhangfeng Zhong

{"title":"Rosa roxburghii Tratt Fruit Juice Ameliorates DSS-Induced Colitis by Improving Intestinal Barrier and Gut Microbiota Independently of Vitamin C","authors":"Wenyue Chen, Dafeng Fang, Lili Zhang, Mingqing Kuang, Mengyao Zhang, Chenxiao Shen, Yi Liu, Hefeng Zhou, Shengpeng Wang, Wenwen Zhao, Yitao Wang, Zhangfeng Zhong","doi":"10.1002/efd2.70084","DOIUrl":"10.1002/efd2.70084","url":null,"abstract":"Rosa roxburghii Tratt (Cili) is a vitamin C-rich fruit with bioactive polyphenols and flavonoids. This study explored the components of Cili fruit juice and investigated its therapeutic potential in a dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) murine model. Our results revealed that Cili fruit juice and its components (vitamin C and kajiichigaside F1) significantly alleviated colitis symptoms. Notably, colitis symptoms improved even when vitamin C-deficient Cili juice was administered. Specifically, Cili fruit juice restored intestinal barrier integrity, regulated inflammatory cytokines (TNF-α, IL-6, IL-8, and IL-12), and modulated gut microbiota dysbiosis by rebalancing Epsilonbacteraeota, Proteobacteria, and Lactobacillus abundances. Furthermore, Cili fruit juice positively modulated cellular metabolic processes and inflammatory pathways. These findings identify Cili fruit juice as a promising natural adjuvant therapy for UC, driven by the potent bioactivity of kajiichigaside F1, offering a novel strategy to enhance intestinal homeostasis and mitigate inflammation.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Millet Bran Bound Phenolic Compounds Suppresses LPS-Induced Inflammatory Response in Macrophages and Liver Injury Mice via TLR4/NF-κB Signaling Pathway 谷糠结合酚类化合物通过TLR4/NF-κB信号通路抑制lps诱导的巨噬细胞和肝损伤小鼠炎症反应

IF 5.7

eFood Pub Date : 2025-07-09 DOI: 10.1002/efd2.70078

Ying-kun Cai, Jin-yue Sun, Ying-ying Chen, Meng-qi Zhang, Shu-tao Sun, Qi-dong Ren, Mu-xuan Wang, Mohamed A. Farag, Bo Zhang, Xu Guo, Chao Liu

{"title":"Millet Bran Bound Phenolic Compounds Suppresses LPS-Induced Inflammatory Response in Macrophages and Liver Injury Mice via TLR4/NF-κB Signaling Pathway","authors":"Ying-kun Cai, Jin-yue Sun, Ying-ying Chen, Meng-qi Zhang, Shu-tao Sun, Qi-dong Ren, Mu-xuan Wang, Mohamed A. Farag, Bo Zhang, Xu Guo, Chao Liu","doi":"10.1002/efd2.70078","DOIUrl":"10.1002/efd2.70078","url":null,"abstract":"Millet bran, rich in bioactive phenolic compounds, holds potential for both nutritional and therapeutic applications. In this study, bound phenolic compounds were isolated from millet bran, yielding a potent fraction named BPS-2. UPLC-MS/MS detected 16 major phenolic compounds in BPS-2. In vitro assays revealed that BPS-2 exerted a significant anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage, as manifested by reduced production of inflammatory mediators (IL-1β, IL-6, and TNF-α) and downregulation of the expression levels of the pro-inflammatory enzymes Cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS). Network pharmacological analysis identified the suppression of the TLR4/NF-κB pathway as the primary mechanism mediating the anti-inflammatory activity of BPS-2, which was validated using the LPS-induced RAW 264.7 macrophage model and liver injury mice model. Western blot analysis revealed that BPS-2 significantly decreased the phosphorylation of IκBα and p65 to regulate the TLR4/NF-κB signaling pathway, thereby exerting anti-inflammatory activity. Molecular docking studies revealed strong interactions between the active compounds of BPS-2 and TLR4 through key amino acid residues, including Pro116, Thr114, and Arg105. These results underscore the potential application of millet bran bound phenolic compounds as naturally occurring anti-inflammatory substances.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"6 4","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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