Halymenia durvillei Extracts Exert Antiobesity Effects by Targeting hosl-1-Mediated Lipolysis in a Glucose-Induced Caenorhabditis elegans Model

IF 5.7 Q2 FOOD SCIENCE & TECHNOLOGY

eFood Pub Date : 2025-06-02 DOI:10.1002/efd2.70065

Kawita Chumphoochai, Sukrit Promtang, Pawanrat Chalorak, Preeyanuch Manohong, Nakorn Niamnont, Montakan Tamtin, Prasert Sobhon, Simon Tuck, Krai Meemon

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Abstract

The antiobesity effect of extracts from the marine algae, Halymenia durvillei (HD), was investigated in a glucose-induced Caenorhabditis elegans model of obesity. Total fat accumulation, triglyceride levels, lifespan, intracellular ROS levels, and the potential mechanism of action of H. durvillei extracts were examined. The present study demonstrated that the ethanol fraction of H. durvillei (HDET) and ethyl acetate fraction of H. durvillei (HDEA) extracts led to a significant reduction in fat accumulation, triglyceride levels, the GFP-labeled dhs-3, a marker for lipid droplets, and the intracellular ROS levels. H. durvillei extracts significantly extended the lifespan of glucose-induced worms. In addition, the mRNA expression of lipolysis-related genes, atgl-1 and hosl-1, showed significant upregulation following treatment with H. durvillei extracts. This finding was supported by RNA interference (RNAi) of atgl-1 and hosl-1, which resulted in disrupting the effect of the H. durvillei extracts on lowering fat accumulation. Furthermore, transcriptomic analysis revealed diverse metabolic activities in glucose-induced worms treated with HDEA, affecting fatty acid metabolism. The results suggested that these extracts provide an antiobesity effect mediated through the lipolysis genes, atgl-1 and hosl-1. H. durvillei-derived extracts may offer valuable insights as functional food ingredients for use in the prevention of obesity.

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在葡萄糖诱导的秀丽隐杆线虫模型中，荆芥提取物通过靶向hosl-1介导的脂解发挥抗肥胖作用

在葡萄糖诱导的秀丽隐杆线虫肥胖模型中，研究了海藻（Halymenia durvillei， HD）提取物的抗肥胖作用。研究了总脂肪积累、甘油三酯水平、寿命、细胞内ROS水平以及杜鹃花提取物的潜在作用机制。本研究表明，杜威花乙醇部分（HDET）和杜威花乙酸乙酯部分（HDEA）提取物可显著降低脂肪积累、甘油三酯水平、gfp标记的dhs-3（脂滴标记物）和细胞内ROS水平。杜威草提取物显著延长了葡萄糖诱导的蠕虫的寿命。此外，杜鹃花提取物处理后，多糖相关基因atgl-1和hosl-1的mRNA表达量显著上调。这一发现得到了atgl-1和hosl-1的RNA干扰（RNAi）的支持，这导致了杜鹃提取物降低脂肪堆积的作用被破坏。此外，转录组学分析显示，经HDEA处理的葡萄糖诱导的蠕虫代谢活动多样，影响脂肪酸代谢。结果表明，这些提取物通过脂解基因atgl-1和hosl-1介导的抗肥胖作用。杜鹃花提取物可能为预防肥胖的功能性食品成分提供有价值的见解。

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来源期刊

eFood food research-

CiteScore

6.00

自引率

0.00%

发文量

期刊介绍： eFood is the official journal of the International Association of Dietetic Nutrition and Safety (IADNS) which eFood aims to cover all aspects of food science and technology. The journal’s mission is to advance and disseminate knowledge of food science, and to promote and foster research into the chemistry, nutrition and safety of food worldwide, by supporting open dissemination and lively discourse about a wide range of the most important topics in global food and health. The Editors welcome original research articles, comprehensive reviews, mini review, highlights, news, short reports, perspectives and correspondences on both experimental work and policy management in relation to food chemistry, nutrition, food health and safety, etc. Research areas covered in the journal include, but are not limited to, the following: ● Food chemistry ● Nutrition ● Food safety ● Food and health ● Food technology and sustainability ● Food processing ● Sensory and consumer science ● Food microbiology ● Food toxicology ● Food packaging ● Food security ● Healthy foods ● Super foods ● Food science (general)