IF 5.7

eFood Pub Date : 2026-04-21 DOI: 10.1002/efd2.70147

Naglaa S. Ashmawy, Ahmed A. Zaky, Noha H. Badr, Ali Korin, Mohamed A. Farag

{"title":"Foodborne Carcinogens: Formation Pathways, Health Risks, and Cutting-Edge Physical and Chemical Prevention Strategies","authors":"Naglaa S. Ashmawy, Ahmed A. Zaky, Noha H. Badr, Ali Korin, Mohamed A. Farag","doi":"10.1002/efd2.70147","DOIUrl":"10.1002/efd2.70147","url":null,"abstract":"Food processing methods, though vital for improving food safety, taste, and shelf life, can unintentionally generate harmful carcinogens. Notable compounds such as acrylamide, heterocyclic amines, polycyclic aromatic hydrocarbons, nitrosamines, and lipid oxidation products often arise from high-temperature cooking techniques like frying, grilling, and baking. These chemicals have been linked to increased cancer risks and are classified by the IARC as probable or possible human carcinogens. This review explores the mechanisms underlying the formation of these carcinogens and their public health implications. It also discusses innovative mitigation strategies, including the use of natural antioxidants from spices, fruits, and vegetables, as well as improved cooking methods like air frying and microwave heating. Emphasis is placed on the effectiveness of natural additives in neutralizing reactive intermediates and inhibiting oxidative reactions. By consolidating existing evidence and highlighting research gaps, this review aims to guide safer food processing practices and reduce dietary exposure to carcinogens.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Equol Alleviates Postmenopausal Metabolic Dysfunction-Associated Steatotic Liver Disease via the GPR30/PI3K Pathway 雌马酚通过GPR30/PI3K途径缓解绝经后代谢功能障碍相关的脂肪变性肝病

IF 5.7

eFood Pub Date : 2026-04-19 DOI: 10.1002/efd2.70148

Hanqiang Cui, Bin Wang, Bo Zou, Guiming Zhang, Zhe Xu, Shuo Li, Jing Xu, Xiangmin Ni, Jian Wang

{"title":"Equol Alleviates Postmenopausal Metabolic Dysfunction-Associated Steatotic Liver Disease via the GPR30/PI3K Pathway","authors":"Hanqiang Cui, Bin Wang, Bo Zou, Guiming Zhang, Zhe Xu, Shuo Li, Jing Xu, Xiangmin Ni, Jian Wang","doi":"10.1002/efd2.70148","DOIUrl":"10.1002/efd2.70148","url":null,"abstract":"The rising prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) in postmenopausal women underscores the need for effective preventive strategies. Equol (Eq) alleviates MASLD, yet its molecular mechanisms remain unknown. This study investigated Eq-activated Nrf2 signaling pathways in postmenopausal MASLD. Molecular-docking simulations were performed to investigate the interaction between Eq and G protein-coupled estrogen receptor 30 (GPR30). Western blotting, qRT-PCR, siRNA were employed to elucidate the mechanisms in the MASLD rat model and NaOL-induced HepG2 cell model. Molecular-docking simulations demonstrated that Eq strongly binds GPR30, enhancing nuclear translocation and the subsequent activation of PI3K/Nrf2. Eq ameliorated lipid accumulation and oxidative stress in NaOL-treated HepG2 cells and the liver tissues of MASLD rats. In vitro, following Nrf2 siRNA treatment, Eq failed to alleviate oxidative stress in NaOL-treated HepG2 cells, indicating that Nrf2 plays a critical role in its antioxidative effects. In vivo, Eq activated Nrf2 signaling and reduced oxidative stress in the livers of MASLD rats, potentially conferring protection against oxidative stress injury in high-fat diet and ovariectomy treated SD rats. Notably, Eq treatment in the model groups significantly enhanced PI3K/Nrf2 phosphorylation. In summary, Eq binds to GPR30 and exerts a protective effect against MASLD by upregulating the PI3K/Nrf2 pathway.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Ferroptosis Biomarkers by Thinned Apple Polyphenols Extract in Acute and Chronic Lung Injuries 苹果多酚提取物对急性和慢性肺损伤中铁下垂生物标志物的调节作用

IF 5.7

eFood Pub Date : 2026-04-16 DOI: 10.1002/efd2.70122

Rosalba Siracusa, Alessia Arangia, Marika Cordaro, Roberta Fusco, Salvatore Cuzzocrea, Giancarlo Aldini, Daniela Impellizzeri, Rosanna Di Paola, Ramona D'Amico

{"title":"Modulation of Ferroptosis Biomarkers by Thinned Apple Polyphenols Extract in Acute and Chronic Lung Injuries","authors":"Rosalba Siracusa, Alessia Arangia, Marika Cordaro, Roberta Fusco, Salvatore Cuzzocrea, Giancarlo Aldini, Daniela Impellizzeri, Rosanna Di Paola, Ramona D'Amico","doi":"10.1002/efd2.70122","DOIUrl":"10.1002/efd2.70122","url":null,"abstract":"Acute and chronic lung injuries are characterized by a large amount of ROS, pro-inflammatory leukocytes and ferroptosis, resulting in alveolar injury. Nowadays, there is great interest in the bioactive compounds obtained from the waste products deriving from agriculture and the food industry. Thinned young apples are particularly rich in polyphenols, more than 10-fold with respect to harvested apples, and have antioxidant and anti-inflammatory activities. Therefore, the aim of this study is to investigate whether modulation of ferroptosis biomarkers by thinned apple polyphenols (TAP) extract was able to reduce lung injuries in two experimental model: acute lung injury (ALI) and bleomycin-induced idiopathic pulmonary fibrosis (IPF). Our results demonstrated that TAP extract was able to inhibit the ferroptosis, modulating the main biomarkers: malondialdehyde, NADPH oxidase-4, glutathione peroxidase 4 and ferritin heavy chain 1. This modulation is probably due to Nrf2 activation, negative regulator of ferroptosis, by TAP extract and consequently activating the production of antioxidant enzymes. This induced histological alteration, cytokines release and fibrosis in the alveoli and lung parenchyma. Collectively, these results demonstrated that TAP extract was able to reduce ferroptosis in acute and chronic lung injury models, suggesting it as a potential biomarker for early diagnosis and intervention timely.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal Abuse and Age of Biomass as Critical Factors for the Viability of Functional Microorganisms in “Fiordilatte” Ice Cream 热滥用和生物质年龄是“Fiordilatte”冰淇淋中功能微生物活力的关键因素

IF 5.7

eFood Pub Date : 2026-04-16 DOI: 10.1002/efd2.70123

Antonio Bevilacqua, Mario Terlizzi, Alessandro De Santis, Maria Rosaria Corbo, Milena Sinigaglia, Angela Racioppo, Pasquale Massimiliano Falcone, Barbara Speranza

{"title":"Thermal Abuse and Age of Biomass as Critical Factors for the Viability of Functional Microorganisms in “Fiordilatte” Ice Cream","authors":"Antonio Bevilacqua, Mario Terlizzi, Alessandro De Santis, Maria Rosaria Corbo, Milena Sinigaglia, Angela Racioppo, Pasquale Massimiliano Falcone, Barbara Speranza","doi":"10.1002/efd2.70123","DOIUrl":"10.1002/efd2.70123","url":null,"abstract":"This study investigated the viability during storage, adhesion properties, and survival during simulated gastrointestinal transit of Lactiplantibacillus plantarum c19 and Bifidobacterium longum subsp. infantis DSM 20088 incorporated into “Fiordilatte” ice-cream, with a focus on the effects of thermal abuse and biomass age. A multifactorial experimental design was applied, including the number of thawing cycles (1–3 cycles at 25°C), their duration (2–6 h), and cell age (15, 24, and 72 h). Biomass age was the most critical factor affecting probiotic survival: 72-h-old cultures showed a significant reduction in viability (approximately 1.0–1.9 log CFU/g) compared to 15- to 24-h-old cultures (p < 0.05). Moreover, thawing/refreezing cycles affected viability only in interaction with cell age. Both strains demonstrated strong resistance during simulated gastrointestinal transit, with final concentrations above 7 log CFU/g, and high adhesion capacity, indicating their potential for stable intestinal colonization. Sensory scores were not affected, and the overall scores were between 7.5 and 8.1 without significant differences due to probiotic addition. Overall, these results confirm that ice cream can serve as an effective delivery matrix for probiotics, provided that critical parameters, such as biomass age and strict control of thermal conditions during production and storage, are carefully managed.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of Gui Zhi Fu Ling Formula in Liver Fibrosis: Metabolomics and Gut Microbiota Analysis 归脂复灵方治疗肝纤维化的机制：代谢组学和肠道菌群分析

IF 5.7

eFood Pub Date : 2026-04-15 DOI: 10.1002/efd2.70149

Xiaoliang Deng, Yanqun Luo, Ying Gao, Yi Su, Haiping Xue, Tao Wu

{"title":"Mechanisms of Gui Zhi Fu Ling Formula in Liver Fibrosis: Metabolomics and Gut Microbiota Analysis","authors":"Xiaoliang Deng, Yanqun Luo, Ying Gao, Yi Su, Haiping Xue, Tao Wu","doi":"10.1002/efd2.70149","DOIUrl":"10.1002/efd2.70149","url":null,"abstract":"The Gui Zhi Fu Ling Formula (GZFLF), a classical traditional Chinese medicine prescription originating from the Shanghan Lun, is traditionally used to promote blood circulation and alleviate blood stasis. Modern medical research has demonstrated its anti-inflammatory and anti-fibrotic effects. This study aimed to identify the therapeutic effect, potential targets and mechanisms of GZFLF in hepatic fibrosis through metabolomics and gut microbiota analysis. By combining these results with literature research, we focused on the metabolite eicosapentaenoic acid (EPA) as a beneficial marker metabolite. Furthermore, marker microbial taxa, including Pseudomonas, were identified from the gut microbiota analysis, which may be relevant to the GZFLF intervention. Finally, we examined the interrelationships among gut microbiota, metabolomics, and serological analyses. This study shows that GZFLF may ameliorate liver fibrosis potentially associated with increased hepatic EPA and gut Pseudomonas abundance. We further speculate a potential correlation between these factors. Further studies will be conducted to validate this hypothesis and elucidate the underlying mechanisms.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bibliometric Analysis of Cubebenes and Related Sesquiterpenes: Natural Occurrence, Biosynthesis, Pharmacological Activities, and In-Silico-Based Future Therapeutic Potential 立方苯和相关倍半萜的文献计量学分析：自然发生、生物合成、药理活性和基于硅的未来治疗潜力

IF 5.7

eFood Pub Date : 2026-04-14 DOI: 10.1002/efd2.70128

Khadija Boualam, Ahmet B. Ortaakarsu, Sohaib Khatib, Mohamed A. Farag, Mansour Sobeh

引用次数: 0

Micronization of Whole Grain Flours: Innovative Approaches for Improving Functional Characteristics and Product Performance 全谷物面粉的微粉化：改进功能特性和产品性能的创新方法

IF 5.7

eFood Pub Date : 2026-04-05 DOI: 10.1002/efd2.70139

Diksha Arora, Kamaljit Kaur, Taranpreet Kaur, Julia Sebastian, Seid Reza Falsafi

{"title":"Micronization of Whole Grain Flours: Innovative Approaches for Improving Functional Characteristics and Product Performance","authors":"Diksha Arora, Kamaljit Kaur, Taranpreet Kaur, Julia Sebastian, Seid Reza Falsafi","doi":"10.1002/efd2.70139","DOIUrl":"10.1002/efd2.70139","url":null,"abstract":"Rising consumer demand for nutrient-dense cereal products has renewed interest in whole-grain ingredients, yet the coarse texture and altered functional behavior of whole-grain flours limit their application in many foods. Micronization, the controlled reduction of particle size by mechanical or thermal–mechanical approaches (e.g., ball, jet, roller, cryogenic, ultrafine, and IR-based methods), can modify flour microstructure and molecular organization. This review investigates recent studies on micronization of whole-grain flours, comparing techniques and summarizing their effects on structural, physicochemical, techno-functional, thermal, and rheological properties, and final product quality. It also evaluates benefits and trade-offs (e.g., improved digestibility and bioactive release vs. excessive starch damage and energy costs), and highlights analytical methods and application-specific optimization strategies. Micronization is a promising, sustainable route to reconcile whole-grain nutrition with consumer-acceptable functionality: appropriately reduced particle size enhances hydration, pasting behavior, bioactive release, and dough handling, improving texture and shelf life in many applications. However, over-micronization can increase damaged starch, weaken starch–protein networks, and impair product quality, so process parameters must be tailored to grain type and intended use. Future work should prioritize scale-up, combined modification strategies (e.g., enzymatic or hydrothermal pairing), and targeted studies of digestibility and glycemic response to fully realize micronization's potential.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Bread Development With Cocoa Shell Powder: Nutritional, Phytochemical, Antioxidant, and Microbiological Properties 可可壳粉功能性面包的开发：营养、植物化学、抗氧化和微生物特性

IF 5.7

eFood Pub Date : 2026-04-05 DOI: 10.1002/efd2.70146

Mohamed Esam Rozek, Emmanuel Duah Osei, Anthony Amotoe-Bondzie, Marcela Slukova, Ivona Jančo, Eva Ivanišová, Miroslava Kačániová, Newlove Akowuah Afoakwah

{"title":"Functional Bread Development With Cocoa Shell Powder: Nutritional, Phytochemical, Antioxidant, and Microbiological Properties","authors":"Mohamed Esam Rozek, Emmanuel Duah Osei, Anthony Amotoe-Bondzie, Marcela Slukova, Ivona Jančo, Eva Ivanišová, Miroslava Kačániová, Newlove Akowuah Afoakwah","doi":"10.1002/efd2.70146","DOIUrl":"10.1002/efd2.70146","url":null,"abstract":"Cocoa shell powder (CSP), an underutilized agro-industrial byproduct often discarded, contributes to waste and economic losses but holds potential for product development. This study explored the use of CSP for wheat-based bread development. Bread was prepared with 0%, 5%, 7.5%, and 10% CSP substitution and evaluated its proximate composition, mineral and potentially toxic metals, phytochemicals, antioxidant activity, microbial load, and antimicrobial properties. The enriched bread had improved fat (1.69%–3.57%), protein (11.46%–11.78%), dietary fiber (8.61%–9.70%), ash (0.75%–1.09%), and key minerals (Ca, K, Mg, Zn, Cu). Toxic elements, including mercury, lead, arsenic, and cadmium, were not detected in any of the bread samples. CSP addition significantly (p < 0.05) resulted in a dose-dependent increase in total polyphenols (138.0–218.0 mg GAE/100 g), flavonoids (85.0–142.0 mg QE/100 g), antioxidant activity and phenolic acid content. The bread samples showed antimicrobial activity against the tested microbes, with a more pronounced inhibitory effect on Pseudomonas aeruginosa. CSP at 7.5%–10% reduced total plate count, coliforms, and yeast/molds, and supported lactic acid bacteria growth. These findings show that CSP enhances the nutritional and functional qualities of bread while serving as an antimicrobial ingredient. The innovative application of CSP in product development underscores its potential for sustainable valorization of cocoa by-products, supporting circular economy principles and enhancing food quality and environmental sustainability.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147667994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary Evaluation on the Anti-Osteoporosis Mechanism of Sojae Semen Germinatum Based on Network Pharmacology and Germination-Improved Bioavailability 基于网络药理学和发芽改善生物利用度的大豆精抗骨质疏松机制初步评价

IF 5.7

eFood Pub Date : 2026-03-31 DOI: 10.1002/efd2.70145

Rezeye Aili, Hong Chen, Feng Qiu

{"title":"Preliminary Evaluation on the Anti-Osteoporosis Mechanism of Sojae Semen Germinatum Based on Network Pharmacology and Germination-Improved Bioavailability","authors":"Rezeye Aili, Hong Chen, Feng Qiu","doi":"10.1002/efd2.70145","DOIUrl":"10.1002/efd2.70145","url":null,"abstract":"Sojae Semen Germinatum is a processed soybean product germinated using mature seeds of Glycine max (L.) Merr. with therapeutic benefits for osteoporosis. However, its action mechanism and related chemical components are unclear. Therefore, hypothesis-generating methods of network pharmacology and molecular docking were adopted to initially predict the potential mechanism of SSG in treating osteoporosis. Subsequently, experimental validation was performed using mesenchymal stem cell (MSC) proliferation assays to verify the hypothesis. The MSC proliferation-promoting activity of soybean isoflavone aglycones was greater than that of soybean isoflavone glycosides (p < 0.05). Additionally, an LC–ESI–MS/MS method was investigated to confirm the bioavailability of those chemical components after oral administration of SSG and soybean to rats. Only small amounts of free daidzein, glycitein, and genistein were detected in rat plasma, while large amounts of them were detected after being hydrolyzed by β–glucosidase, indicating the main forms of isoflavones were their corresponding phase II metabolites. The bioavailability of total daidzein, glycitein, and genistein increased during soybean germination, reaching 143 ± 30%, 184 ± 32%, and 130 ± 24%, respectively. Soybeans are processed into SSG through germination, which increases the contents of soy isoflavone aglycones, thereby improving their bioavailability and enhancing their therapeutic effect on osteoporosis.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://iadns.onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147684298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Antimicrobial–Antioxidant Postbiotics From Lactiplantibacillus plantarum for Clean-Label Meat Preservation 用于清洁标签肉类保鲜的植物乳杆菌双抗微生物-抗氧化后生制剂

IF 5.7

eFood Pub Date : 2026-03-26 DOI: 10.1002/efd2.70140

Florentina Badea, Mircea-Cosmin Pristavu, Filofteia Camelia Diguță, Alexandru Constantin Aldea, Mihaela Dragoi Cudalbeanu, Simona Marcu Spinu, Alina Ortan, Marian Nicolae Verziu, Florentina Matei

{"title":"Dual Antimicrobial–Antioxidant Postbiotics From Lactiplantibacillus plantarum for Clean-Label Meat Preservation","authors":"Florentina Badea, Mircea-Cosmin Pristavu, Filofteia Camelia Diguță, Alexandru Constantin Aldea, Mihaela Dragoi Cudalbeanu, Simona Marcu Spinu, Alina Ortan, Marian Nicolae Verziu, Florentina Matei","doi":"10.1002/efd2.70140","DOIUrl":"10.1002/efd2.70140","url":null,"abstract":"The rising consumer demand for clean-label foods has intensified the search for natural alternatives to synthetic preservatives. Postbiotics from lactic acid bacteria are safe and multifunctional, but their effectiveness in real food systems remains unclear. This study examined the biochemical composition, functional properties, and biopreservation potential of cell-free supernatants (CFSs) produced by two Lactiplantibacillus plantarum strains (MI131 and MI207). Compositional analyses by FTIR, GC–MS, and UHPLC identified diverse bioactive metabolites. GC–MS detected 25 metabolites, with MI131 being more abundant. UHPLC confirmed the presence of phenolic acids and flavonoids, which are known for their antioxidant activity. Antioxidant activity, measured by DPPH assay, was similar for both strains. Antibacterial tests showed strain-specific effects: MI131 exhibited a broader inhibition spectrum, whereas MI207 had lower minimum bactericidal concentrations against Listeria monocytogenes and Salmonella Typhimurium (6.25 mg/mL). Applying CFS to meat stored for 15 days at 4°C reduced viable counts (< 3 log CFU/g) and inhibited pathogens, as confirmed by culture-based methods and qPCR. The results support the use of postbiotics from L. plantarum strains MI131 and MI207 as natural biopreservatives that extend shelf life, promote clean-label formulations, enhance safety, and reduce reliance on synthetic antimicrobials, thereby aligning with EU regulations.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 2","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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