Exploring the multifaceted therapeutic potentials of Brassaiopsis palmata (leaves) through in-vitro and in-vivo approaches

IF 4 Q2 FOOD SCIENCE & TECHNOLOGY
eFood Pub Date : 2024-10-06 DOI:10.1002/efd2.70009
Mohidul Islam, Fowzul Islam Fahad, Ahasanul Karim, Arifa Sultana, A. T. M. Mostafa Kamal, Md. Sohel Rana, Mohammad Nazmul Islam
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Abstract

Brassaiopsis palmata, an androgynous tree from Araliaceae family, has been widely found in the Asian subcontinent, and reported to have potentiality against skin infection, and many therapeutic properties still unidentified. Here, our current investigation aims to discover the innovative pharmacological potentials of the methanol extract of B. palmata leaves (MEBPL) through in-vivo and in-vitro approaches. Several secondary metabolites were revealed throughout the qualitative phytochemical screening of the plant extracts. MEBPL exhibited strong radical scavenging properties (IC50 178.13 µg/mL) through the 2, 2-diphenyl-1-picryhydrazyl (DPPH) assay, and through the quantitative (phenolic and flavonoid) assays with a moderate (LD50 153.14 µg/mL) toxic effect. In anti-inflammatory screening, MEBPL showed significant dose dependent inhibitory activity; and the peak inhibition were found 78.01 ± 1.22% and 82.46 ± 1.52% at 1000 µg/mL concentration on hypotonic-induce RBC hemolysis & protein denaturation test respectively. Moreover, the plant extracts manifested moderate percentage of clot lysis (28.24 ± 2.50%) on the investigation of thrombolytic assay. The anti-nociceptive activity of MEBPL was analyzed through acetic acid and formalin induce pain tests. Both 200 and 400 mg/kg dose of MEBPL exerted significant (p ˂ 0.001) dose depending depletion of acetic acid induced writhing and formalin stimulated licking test which indicated strong analgesic properties of plant extracts. In addition, the outcomes of anti-depressant evaluation suggested that treatment with both 200 and 400 mg/kg doses showed potential dose depending activity on both FST and TST model. Furthermore, the plant extracts manifested dose dependent reduction of anxiety like behaviors in the rodent model. Particularly, mice administrated with 400 mg/kg dose of MEBPL significantly (p ˂ 0.05) enhanced the percentage of entries and time spent in the open arm in EPM, and also showed the highest amount of head dipping tendency in HBT. In contrast, the outcomes of this research suggest that B. palmata could be another source of antioxidant, cytotoxic, anti-inflammatory, thrombolytic, anti-nociceptive, anti-depressant, and anxiolytic agents. Further research on the mechanisms underlying bioactivities is required.

通过体外和体内方法探索拟南芥(叶片)的多方面治疗潜力
棕榈十字花科(Brassaiopsis palmata)是一种雌雄同株的树木,广泛分布于亚洲次大陆,据报道具有抗皮肤感染的潜力,但许多治疗特性仍未确定。我们目前的研究旨在通过体内和体外方法发现棕榈叶甲醇提取物(MEBPL)的创新药理潜力。通过对植物提取物进行定性植物化学筛选,发现了几种次生代谢物。通过 2,2-二苯基-1-苦基肼(DPPH)检测和定量(酚类和类黄酮)检测,MEBPL 表现出很强的自由基清除能力(IC50 178.13 µg/mL),毒性作用适中(LD50 153.14 µg/mL)。在抗炎筛选中,MEBPL 表现出显著的剂量依赖性抑制活性;在 1000 µg/mL 浓度时,对低渗诱导的红细胞溶血& 和蛋白质变性试验的抑制峰值分别为 78.01 ± 1.22% 和 82.46 ± 1.52%。此外,在血栓溶解试验中,植物提取物表现出中等程度的血栓溶解率(28.24 ± 2.50%)。醋酸和福尔马林诱导疼痛试验分析了 MEBPL 的抗痛觉活性。200毫克和400毫克/千克剂量的MEBPL都能显著(p ˂ 0.001)抑制醋酸诱导的蠕动和福尔马林刺激的舔舐试验,这表明植物提取物具有很强的镇痛特性。此外,抗抑郁评估结果表明,200 毫克/千克和 400 毫克/千克的剂量对 FST 和 TST 模型都显示出潜在的剂量活性。此外,在啮齿动物模型中,植物提取物对焦虑行为的减少也表现出剂量依赖性。特别是,给小鼠注射 400 毫克/千克剂量的 MEBPL 能显著(p ˂ 0.05)提高小鼠在 EPM 中的进入比例和张开手臂的时间,还能在 HBT 中表现出最高的头部下垂倾向。相比之下,本研究的结果表明,棕榈叶可作为抗氧化、细胞毒性、抗炎、溶栓、抗痛觉、抗抑郁和抗焦虑剂的另一种来源。有关生物活性的机制还需要进一步研究。
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来源期刊
eFood
eFood food research-
CiteScore
6.00
自引率
0.00%
发文量
44
期刊介绍: eFood is the official journal of the International Association of Dietetic Nutrition and Safety (IADNS) which eFood aims to cover all aspects of food science and technology. The journal’s mission is to advance and disseminate knowledge of food science, and to promote and foster research into the chemistry, nutrition and safety of food worldwide, by supporting open dissemination and lively discourse about a wide range of the most important topics in global food and health. The Editors welcome original research articles, comprehensive reviews, mini review, highlights, news, short reports, perspectives and correspondences on both experimental work and policy management in relation to food chemistry, nutrition, food health and safety, etc. Research areas covered in the journal include, but are not limited to, the following: ● Food chemistry ● Nutrition ● Food safety ● Food and health ● Food technology and sustainability ● Food processing ● Sensory and consumer science ● Food microbiology ● Food toxicology ● Food packaging ● Food security ● Healthy foods ● Super foods ● Food science (general)
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