A novel arabinogalactan extracted from Epiphyllum oxypetalum (DC.) Haw improves the immunity and gut microbiota in cyclophosphamide-induced immunosuppressed mice

IF 4 Q2 FOOD SCIENCE & TECHNOLOGY
eFood Pub Date : 2024-10-04 DOI:10.1002/efd2.70002
Jin Dai, Zhiwei Zhou, Liangwei Chen, Shujuan Cao, Ke Luo, Jinmei Zhang, András Dinnyés, Dan Wang, Qun Sun
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Abstract

A novel type I arabinogalactan (AG-I) polysaccharide (EPS) from Epiphyllum oxypetalum (DC.) Haw's flowers is hypothesized to possess immunomodulatory activity. This study investigated EPS's effects on immune functions and its potential mechanism for enhancing intestinal health in immunosuppressed mice. The results showed that supplementing EPS significantly alleviated immune organ damage, increased the thymus index (p < 0.01), and regulated the key immune factors, including the tumor necrosis factor-alpha (TNF-α), immunoglobulin A (IgA), and complement 3 (C3) in the liver (p < 0.05). EPS promoted the expression of intestinal immune barrier and chemical barrier proteins such as interferon-γ (IFN-γ) and mucin 2 (MUC2) (p < 0.05), effectively repairing intestinal damage. EPS improved the diversity and structure of intestinal microbiota in immunosuppressed mice (p < 0.05) and significantly altered the abundance of intestinal immune-related microbial taxa, including Lactobacillaceae and Lactobacillus (p < 0.01). Furthermore, EPS supplementation altered intestinal lactic acid metabolism, significantly increasing lactic acid levels by up to 3.4-fold (p < 0.01), and enhanced the expression of Gpr81, Wnt3a, and β-catenin proteins at the bottom of the colonic crypts, which may repair the intestinal physical barrier. Overall, EPS represents a novel AG-I immunomodulatory dietary polysaccharide that enhances immunity and improves gut health.

从 Epiphyllum oxypetalum (DC.) Haw 中提取的一种新型阿拉伯半乳聚糖可改善环磷酰胺诱导的免疫抑制小鼠的免疫力和肠道微生物群
一种从 Epiphyllum oxypetalum (DC.) Haw 的花中提取的新型 I 型阿拉伯半乳聚糖(AG-I)多糖(EPS)被认为具有免疫调节活性。本研究调查了 EPS 对免疫功能的影响及其增强免疫抑制小鼠肠道健康的潜在机制。结果表明,补充 EPS 能明显减轻免疫器官损伤,提高胸腺指数(p < 0.01),并调节关键免疫因子,包括肝脏中的肿瘤坏死因子-α(TNF-α)、免疫球蛋白 A(IgA)和补体 3(C3)(p < 0.05)。EPS 促进了肠道免疫屏障和化学屏障蛋白的表达,如干扰素-γ(IFN-γ)和粘蛋白 2(MUC2)(p < 0.05),有效修复了肠道损伤。EPS 改善了免疫抑制小鼠肠道微生物群的多样性和结构(p < 0.05),并显著改变了肠道免疫相关微生物类群的丰度,包括乳杆菌科和乳酸杆菌(p < 0.01)。此外,补充 EPS 改变了肠道乳酸代谢,使乳酸水平显著增加了 3.4 倍(p <0.01),并增强了结肠隐窝底部 Gpr81、Wnt3a 和 β-catenin 蛋白的表达,这可能会修复肠道物理屏障。总之,EPS 是一种新型的 AG-I 免疫调节膳食多糖,可增强免疫力并改善肠道健康。
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来源期刊
eFood
eFood food research-
CiteScore
6.00
自引率
0.00%
发文量
44
期刊介绍: eFood is the official journal of the International Association of Dietetic Nutrition and Safety (IADNS) which eFood aims to cover all aspects of food science and technology. The journal’s mission is to advance and disseminate knowledge of food science, and to promote and foster research into the chemistry, nutrition and safety of food worldwide, by supporting open dissemination and lively discourse about a wide range of the most important topics in global food and health. The Editors welcome original research articles, comprehensive reviews, mini review, highlights, news, short reports, perspectives and correspondences on both experimental work and policy management in relation to food chemistry, nutrition, food health and safety, etc. Research areas covered in the journal include, but are not limited to, the following: ● Food chemistry ● Nutrition ● Food safety ● Food and health ● Food technology and sustainability ● Food processing ● Sensory and consumer science ● Food microbiology ● Food toxicology ● Food packaging ● Food security ● Healthy foods ● Super foods ● Food science (general)
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