eFood最新文献

{"title":"Application of nanotechnology in dairy desserts and ice cream formulation with the emphasize on textural, rheological, antimicrobial, and sensory properties","authors":"Neda Aliabbasi,&nbsp;Zahra Emam-Djomeh","doi":"10.1002/efd2.170","DOIUrl":"https://doi.org/10.1002/efd2.170","url":null,"abstract":"<p>Nanotechnology has emerged as a promising approach for improving the texture, rheology, and antimicrobial aspects of dairy desserts and ice cream. This study presents an overview of how nanotechnology is applied in developing these dairy products, focusing on its impact on texture, flow characteristics, and antimicrobial properties. Furthermore, nanomaterials are crucial in enhancing sensory aspects like taste and overall consumer appeal. The integration of nanostructures in dairy products can modify the structural and functional characteristics of these products, leading to improvements in texture and being nutritious. Moreover, incorporation of nano materials in dairy products could enhance rheological properties by improving emulsion stability and consistency, modifying material viscosity to influence flow behavior, and easily interacting with other components in the product matrix, thereby impacting properties such as creaminess and mouthfeel. Antimicrobial nanomaterials with elevated surface-to-volume ratio. play a crucial role in preventing spoilage and ensuring product safety. The potential advantages and challenges linked to using nanotechnology in dairy desserts and ice cream are examined, highlighting the importance of further research in this evolving field.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141326684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An updated review of composition, health benefits, and applications of phenolic compounds in Ficus Carica L.","authors":"Qinqiu Zhang,&nbsp;Yue Peng,&nbsp;Fan Li,&nbsp;Yi Xu,&nbsp;Qing Zhang,&nbsp;Dingtao Wu,&nbsp;Mingrui Chen,&nbsp;Shang Lin,&nbsp;Wen Qin","doi":"10.1002/efd2.154","DOIUrl":"https://doi.org/10.1002/efd2.154","url":null,"abstract":"<p><i>Ficus carica L</i>. (FC) is rich in phenolic compounds such as phenolic acids, flavonoids, and anthocyanins. Phenolic compounds in FC show remarkable health benefits and applications. The composition of phenolic compounds in FC can vary with some factors, such as variety, ripeness, geographical location, extraction methods and processing methods. New techniques such as supercritical fluid extraction, subcritical water extraction and ionic liquid extraction should be further developed and combined with traditional techniques to obtain higher quality sources of phenolic compounds. Numerous in vivo and in vitro studies reported healthy benefits of phenolic compounds in FC, such as anti-oxidant, anti-inflammatory, anti-diabetic, anti-obesity, anti-cancer and anti-Alzheimer's. Further works are required to reveal mechanisms and structure-activity relationships, such as targeted metabolomics and pharmacological studies. In terms of applications, phenolic compounds in FC as prebiotics on the intestinal flora need to be further studied to expand their applicability in food fermentation. In a word, this review highlights the remarkable health benefits and application value of phenolic compounds in FC.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141298632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring storage characteristics of gray mold inoculated strawberries treated with limonene liposome and oxygen absorbers and prediction of sensory shelf-life with UV-VIS-NIR reflectance","authors":"Prabesh Joshi,&nbsp;Ruplal Choudhary,&nbsp;Punit Kohli,&nbsp;Prachi Pahariya,&nbsp;Annie Y. Vargas-Lizarazo","doi":"10.1002/efd2.174","DOIUrl":"https://doi.org/10.1002/efd2.174","url":null,"abstract":"<p>In this research, the effect of grey mold inoculation and subsequent postharvest treatment with limonene-liposomes and oxygen absorbers is observed on the storage characteristics of strawberries. The survival analysis of the end of sensory shelf-life indicated that inoculated strawberries were 9.96 times more likely to be rejected compared to uninoculated strawberries. The inoculation also resulted in lower firmness, higher rate of weight loss, higher CO₂ respiration rates, and higher total polyphenol contents. Among the inoculated strawberries, limonene-liposome treated strawberries had a longer average shelf-life by 1.5 days compared to control inoculated strawberries. Similarly, oxygen absorber treated strawberries had lower respiration rates and lower rate of weight loss. The sequential addition of variables in the multiple linear models fitting days till rejection (DTR) show that up to 52% of the variation in DTR can be explained from days of storage, state of inoculation, firmness, respiration rate and total anthocyanin content. The models built from storage characteristics other than state of inoculation can explain up to 26% of variation in DTR. The partial least square regression (PLSR) models for the prediction of DTR built from two different types of spectral data, UV-VIS and NIR, yielded evaluation R² up to 0.778 and 0.765 respectively.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141298625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the effects of intestinal FXR agonists and antibiotics on the regulation of red kidney bean polysaccharides in the liver metabolism in mice with type 2 diabetes","authors":"Yunhui Zhang,&nbsp;Caili Qi,&nbsp;Xingguo Li,&nbsp;Mengyuan Geng,&nbsp;Honglin Lan,&nbsp;Yifang Wei,&nbsp;Denglin Luo,&nbsp;Zhouya Bai,&nbsp;Jinying Guo,&nbsp;Sihai Han","doi":"10.1002/efd2.159","DOIUrl":"https://doi.org/10.1002/efd2.159","url":null,"abstract":"<p>Our previous study revealed that red kidney bean polysaccharides (RKB) exhibit a pronounced hypoglycemic effect on type 2 diabetic rats, while simultaneously exerting a significant ameliorative impact on hepatic damage in these animals. However, the precise mechanism underlying the effects of RKB on diabetes and liver metabolism remains unproven. In this study, we utilized a mouse model of type 2 diabetes induced by a high-fat diet combined with streptozotocin to investigate the impact of RKB. We administered a combined intervention involving antibiotics, fexaramine, and RKB to elucidate the mechanism underlying RKB's effects. Our findings demonstrated that RKB significantly ameliorated liver function indices and histopathological injuries. Nevertheless, when antibiotics and fexaramine were introduced as interventions, they hindered the beneficial effects of RKB on liver function in type 2 diabetic mice. Furthermore, our nontargeted metabolomics analysis revealed that antibiotics and fexaramine exerted their inhibitory actions on RKB efficacy through modulation of distinct metabolites involved in glycerophospholipid and purine metabolic pathways.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for the in vitro determination of anti-inflammatory activity","authors":"Xiujing Huang,&nbsp;Yue Li,&nbsp;Maidinai Sabier,&nbsp;Jinping Si,&nbsp;Pan Wang,&nbsp;Yanghui Shen,&nbsp;Xinfeng Zhang,&nbsp;Jingjing Liu","doi":"10.1002/efd2.160","DOIUrl":"https://doi.org/10.1002/efd2.160","url":null,"abstract":"<p>Chronic inflammation contributes to various chronic diseases in humans. Currently used anti-inflammatory drugs have several limitations and are not suitable for long-term use. Anti-inflammatory foods or extracts can prevent inflammation-related diseases, offering a promising approach for the management of these pathologies. In vitro anti-inflammatory experiments have the advantages of short experimental period and low cost for screening anti-inflammatory foods or drugs. They measure pro-inflammatory enzyme activity such as lipoxygenase (LOX) and cyclooxygenase (COX), and the production of pro-inflammatory cytokines such as tumor necrosis factor (TNF) and interleukins (IL). This article reviews in vitro assays used to evaluate the anti-inflammatory effect, examining the effects of different inflammatory factors and the employed methods, and analyzing their principles, advantages, and disadvantages. Thus, it aims to provide guidelines for screening natural foods with anti-inflammatory potential.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loquat and its phytochemical potential: A promising application in food technology","authors":"Vishal Kumar,&nbsp;Prerna Gupta,&nbsp;Mukul Kumar","doi":"10.1002/efd2.158","DOIUrl":"https://doi.org/10.1002/efd2.158","url":null,"abstract":"<p>This review study highlights the importance of loquat (<i>Eriobotrya japonica</i> Lindl.) in terms of its nutritional, pharmaceutical, and nutraceutical value. The entire plant, including its seeds, leaves, flowers, fruits, and peel, contains a wide range of bioactive compounds, such as phenolics, carotenoids, triterpenic acids, and organic acids. These compounds exhibit a wide range of beneficial properties such as fighting cancer, reducing inflammation, managing diabetes, combating viruses, preventing mutations, inhibiting nitrosamine formation, killing harmful microorganisms, lowering blood sugar levels, inducing cell death, and protecting the liver. The extraction and separation of such compounds will greatly enhance and expand its potential. Furthermore, the waste generated from the seeds and peel can be effectively used for submerged and solid state biomass production.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential use of natural killer cells to treat Alzheimer's disease: Can nutrition play a role?","authors":"Athena Dong,&nbsp;Yi-Wen Huang,&nbsp;Huizhi Chen,&nbsp;Ruiling Liu,&nbsp;Weijie Wu,&nbsp;Haiyan Gao,&nbsp;Chin-Kun Wang,&nbsp;Jianhua Yu,&nbsp;Li-Shu Wang","doi":"10.1002/efd2.145","DOIUrl":"https://doi.org/10.1002/efd2.145","url":null,"abstract":"&lt;p&gt;According to the World Health Organization (WHO), dementia is a significant cause of disability and dependency among older individuals globally (Wortmann, &lt;span&gt;2012&lt;/span&gt;). Among the various types of dementia, Alzheimer's disease (AD) is the most prevalent, accounting for over 60% of cases (&lt;span&gt;2020&lt;/span&gt;). It is important to note that AD cases can be categorized into two main types: early-onset familial AD, which accounts for less than 1% of cases, and late-onset sporadic AD, which comprises approximately 95% of cases (Drummond &amp; Wisniewski, &lt;span&gt;2017&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;The hallmark characteristics of AD are the presence of extracellular amyloid β (Aβ) plaques and intraneuronal phosphorylated tau (p-Tau) aggregates, known as neurofibrillary tangles (Yeh et al., &lt;span&gt;2017&lt;/span&gt;). Neurons in the brain produce significant amounts of Aβ (Busciglio et al., &lt;span&gt;1993&lt;/span&gt;). Microglia, which are the macrophages of the brain and play a substantial role in neuroinflammation, bind to the Aβ peptide to help clear it from the brain (Yeh et al., &lt;span&gt;2017&lt;/span&gt;). APOE4 and TREM2*R47H are two crucial genetic risk factors in the late-onset sporadic AD population (Le Guen et al., &lt;span&gt;2022&lt;/span&gt;; Guerreiro et al., &lt;span&gt;2013&lt;/span&gt;; Jonsson et al., &lt;span&gt;2013&lt;/span&gt;). These genetic variants contribute to impaired uptake of Aβ and the subsequent accumulation of Aβ (Yeh et al., &lt;span&gt;2017&lt;/span&gt;), a critical molecule involved in the initiation of neuronal degeneration (Leng &amp; Edison, &lt;span&gt;2021&lt;/span&gt;). The accumulation of Aβ activates the phagocytic function of microglia to degrade Aβ and triggers pro-inflammatory pathways (Leng &amp; Edison, &lt;span&gt;2021&lt;/span&gt;). Moreover, activated microglia release reactive oxygen species and pro-inflammatory cytokines, leading to neuronal dysfunction and progressive cell death as the disease advances (Alawieyah Syed Mortadza et al., &lt;span&gt;2018&lt;/span&gt;; Combs et al., &lt;span&gt;2001&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;Multiple phase 3 human clinical trials targeting Aβ in early-stage AD patients (mild cognitive impairment or mild dementia due to AD) have been conducted around the world; however, showing discouraging results with respect to Bapineuzumab (a humanized anti-amyloid-beta monoclonal antibody) (Salloway et al., &lt;span&gt;2014&lt;/span&gt;), Lecanemab (a humanized IgG1 monoclonal antibody that binds with high affinity to Aβ soluble protofibrils) (van Dyck et al., &lt;span&gt;2023&lt;/span&gt;), Aducanumab (a human monoclonal antibody directed against aggregated soluble and insoluble forms of amyloid beta) (Budd Haeberlein et al., &lt;span&gt;2022&lt;/span&gt;), Crenezumab (a humanized monoclonal immunoglobulin G4 antibody targeting β-amyloid oligomers) (Ostrowitzki et al., &lt;span&gt;2022&lt;/span&gt;), Gantenerumab (a fully human monoclonal antibody that binds aggregated Aβ and removes Aβ plaques by Fc receptor-mediated phagocytosis) (Ostrowitzki et al., &lt;span&gt;2017&lt;/span&gt;), and Solanezumab (a humanized monoclonal antibody, preferentially binds soluble forms of amyloi","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent research progress in the plant contribution to the management of tuberculosis","authors":"Elwira Sieniawska","doi":"10.1002/efd2.157","DOIUrl":"https://doi.org/10.1002/efd2.157","url":null,"abstract":"&lt;p&gt;Tuberculosis is an infectious disease caused by &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt;. Despite the successes in the development of diagnosis and treatment of this disease, it still poses a significant threat to humanity, especially in countries with an average and low level of healthcare development (Maiolini, &lt;span&gt;2020&lt;/span&gt;). The newest World Health Organization, Global Tuberculosis Report (&lt;span&gt;2023&lt;/span&gt;) revealed that the reported global number of people newly diagnosed with TB was 7.5 million in 2022. This is the highest number since WHO began global TB monitoring in 1995, above the pre-COVID baseline. Globally in 2022, &lt;i&gt;M. tuberculosis&lt;/i&gt; caused an estimated 1.30 million deaths. Also, the number of people with multidrug-resistant(MDR) or rifampicin-resistant tuberculosis increased with 450,000 new cases (World Health Organization, Global Tuberculosis Report, &lt;span&gt;2023&lt;/span&gt;). Moreover, the last decade has seen an increase in the number of cases of extremely drug-resistant tuberculosis (XDR-TB), defined as MDR-TB with additional resistance to at least one of the fluoroquinolones and one of the drugs administered in the form of injections (amikacin, kanamycin, capreomycin) used in MDR-TB treatment regimens. In the era of global population movements, the emergence of MDR-TB and XDR-TB makes the fight against tuberculosis an ongoing challenge (World Health Organization, Global Tuberculosis Report, &lt;span&gt;2019&lt;/span&gt;). Current treatment recommendations require the use of combinations of different drugs for periods ranging from 6 months to 9–20 months (MDR-TB). However, in the case of XDR tuberculosis, or when the treatment results are not satisfactory, the duration of treatment may be much longer (World Health Organization, Global Tuberculosis Report, &lt;span&gt;2019&lt;/span&gt;). Failure in antituberculosis therapy is caused mainly by its long duration, complicated drug regimens, strong side effects of antituberculosis drugs, and their interactions with drugs used in other disease units (Shehzad, &lt;span&gt;2013&lt;/span&gt;). What is more, in 2021, for the first time in 9 years, the estimated number of deaths from tuberculosis increased, which was caused by limited access to health care due to the COVID-19 pandemic. As a result, an estimated half of the infections went undiagnosed and untreated. The report also indicates that there has been a reduction in funding for tuberculosis treatment and BCG vaccines in children. The COVID-19 pandemic outbreak in 2020 disrupted essential health services and placed an additional burden on people with tuberculosis (World Health Organization, Global Tuberculosis Report, &lt;span&gt;2021&lt;/span&gt;). Nevertheless, the fight against tuberculosis is still ongoing and plant products play an important role in it.&lt;/p&gt;&lt;p&gt;Plants are essential for human living. Besides providing food for people and feed for animals, plants create a natural environment, produce oxygen, and supply various raw materials. Since ancient times people used plant","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myricetin and dihydromyricetin as JNK1 inhibitors protect against ROS-mediated oxidative stress and apoptosis","authors":"Rili Hao,&nbsp;Xinyu Song,&nbsp;Yajie Li,&nbsp;Xinru Lin,&nbsp;Hui Guan,&nbsp;Dongxiao Sun-Waterhouse,&nbsp;Dapeng Li","doi":"10.1002/efd2.155","DOIUrl":"https://doi.org/10.1002/efd2.155","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 <p>Reactive oxygen species (ROS)-mediated oxidative stress and apoptosis contribute to the pathogenesis and pathological conditions of various diseases induced by food and environmental pollutants. Myricetin and dihydromyricetin, as natural flavonoids, can counteract oxidative damage, apoptosis, and associated diseases. Molecular docking analysis, ELISA, fluorescence spectroscopy, and Western blot techniques were applied to examine the potential of the two flavonoids as effective therapeutics for ROS-mediated diseases and the mechanism(s) underlying their protective effects against ethanol/H₂O₂-induced damage. In Kunming mice and HepG2 cells, myricetin and dihydromyricetin inhibited ethanol/H₂O₂-induced liver damage, ROS accumulation, and oxidative stress and apoptosis. Molecular docking showed that myricetin and dihydromyricetin interacted with JNK1 via hydrophobic interactions and hydrogen bonds, thereby exerting their protective effects. The significant differences in their binding patterns to JNK1 resulted from the different bond types between C2 and C3 on their C ring. Myricetin and dihydromyricetin likely protected liver injury via counteracting ROS/JNK1-mediated oxidative stress and apoptosis. The finding that the suppression of ROS-mediated oxidative stress and apoptosis by flavonoids via interacting with functional proteins, such as kinase, may represent an alternative strategy for treating excessive ROS-induced health problems.</p>\u0000 </section>\u0000 </div>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed-culture fermentation of lactic acid bacteria and yeast","authors":"Jie Gao,&nbsp;Chao Xu,&nbsp;Guifang Tian,&nbsp;Yue Hong Ji,&nbsp;Faizan A. Sadiq,&nbsp;Ke Wang,&nbsp;Yaxin Sang","doi":"10.1002/efd2.153","DOIUrl":"https://doi.org/10.1002/efd2.153","url":null,"abstract":"<p>The presence of stone cells in pears is recognized as a problem for the pear processing industry. <i>Bacillus</i> and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study <i>Lactoplantibacillus plantarum</i> JYLP-326 was used in single culture or in the mixed culture with <i>Saccharomyces cerevisiae</i> Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed-culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β-glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed-culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed-culture fermentation using the above-mentioned strains could be exploited by the pear processing industry to degrade stone cells.</p>","PeriodicalId":11436,"journal":{"name":"eFood","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}