IF 5.7

eFood Pub Date : 2026-05-18 DOI: 10.1002/efd2.70165

Yaseen Galali, Pary Hadi, Arkan Mohammed Hassan, Holem Hashm Balaky, Tavga Sulaiman Rashid, Bashdar Abuzed Sadee, Tanya Salam Salih, Hamed Hassanzadeh

{"title":"Application of Artificial Intelligence in Food Science and Nutrition: Challenges and Future Perspectives","authors":"Yaseen Galali, Pary Hadi, Arkan Mohammed Hassan, Holem Hashm Balaky, Tavga Sulaiman Rashid, Bashdar Abuzed Sadee, Tanya Salam Salih, Hamed Hassanzadeh","doi":"10.1002/efd2.70165","DOIUrl":"10.1002/efd2.70165","url":null,"abstract":"The Artificial intelligence (AI) and Machine Learning (ML) advent has proclaimed unparalleled knowledge transformation across several sectors, including food science and nutrition. This advancement can aid in a deeper understanding of the insights of both fields and their subfields. A systematic search was conducted across literature resources in many databases, including Web of Science, Scopus, and PubMed, according to PRISMA guidelines. The possible eligible study data were retrieved to assess eligibility and inclusion criteria. This research comprehensively explores the use AI applications in food science, such as the food industry and processing, food safety and packaging, and nutrition, including food and nutrient intake, supplements, clinical nutrition, gut microbiota, and trace elements intake. AI applications can be very helpful in addressing various issues, developing novel techniques in food production, food safety, and quality, and aiding in planning nutrition and nutrient intake for better health with high accuracy and precision. Despite these advancements in the application of AI and ML in both food science and nutrition, more improvement is needed for more efficient, precise, and accurate application in some fields.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148048735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Characterization of Ginseng Adventitious Root Polysaccharides and Their Effects on Ameliorating Insulin Resistance 人参不定根多糖的结构特征及其改善胰岛素抵抗的作用

IF 5.7

eFood Pub Date : 2026-05-15 DOI: 10.1002/efd2.70157

Tian-fu Yang, Ting-ting Hu, Xiao-di Zhuang, Yi-lun Zhou, Xin Qi, Cheng-bi Cui

{"title":"Structural Characterization of Ginseng Adventitious Root Polysaccharides and Their Effects on Ameliorating Insulin Resistance","authors":"Tian-fu Yang, Ting-ting Hu, Xiao-di Zhuang, Yi-lun Zhou, Xin Qi, Cheng-bi Cui","doi":"10.1002/efd2.70157","DOIUrl":"10.1002/efd2.70157","url":null,"abstract":"Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (T2DM). Although ginseng polysaccharides have been reported to alleviate IR, the biological functions and mechanisms by which ginseng adventitious root polysaccharides (GARP) improve insulin resistance remain unclear. This study aimed to characterize purified GARP and investigate its effects on insulin resistance and underlying mechanisms. GARP was prepared by hot water extraction, ethanol precipitation, and column chromatography, followed by structural characterization. Its effects were evaluated in IR-HepG2 cells and high-fat diet (HFD)- and streptozotocin (STZ)-induced insulin-resistant mice. After GARP treatment, metabolic parameters, liver pathology, oxidative stress, and the Nrf2/Keap1 pathway were assessed. GARP was identified as a glucose-rich acidic polysaccharide. GARP improved glucose utilization, lipid metabolism, and oxidative stress in vitro and in vivo, alleviated liver injury, and ultimately ameliorated insulin resistance, potentially through activation of the Nrf2/Keap1 signaling pathway. Following extraction and purification, GARP showed a relatively uniform molecular weight distribution and alleviated insulin resistance by improving glycolipid metabolism and redox balance, indicating its potential as a functional food ingredient.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148048585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethnomedicinal Properties and Technological Challenges of Pomegranate Fruit as a Value-Added Product 石榴果作为增值产品的民族药材特性及工艺挑战

IF 5.7

eFood Pub Date : 2026-05-14 DOI: 10.1002/efd2.70163

Muhammad Tayyab Arshad, Farhang Hameed Awlqadr, Sammra Maqsood, Hatem A. Al-Aoh, Md. Sakhawot Hossain, Ali Ikram

{"title":"Ethnomedicinal Properties and Technological Challenges of Pomegranate Fruit as a Value-Added Product","authors":"Muhammad Tayyab Arshad, Farhang Hameed Awlqadr, Sammra Maqsood, Hatem A. Al-Aoh, Md. Sakhawot Hossain, Ali Ikram","doi":"10.1002/efd2.70163","DOIUrl":"10.1002/efd2.70163","url":null,"abstract":"The nutrient-rich pomegranate (Punica granatum) is widely recognized for its historical value and health benefits. Pomegranates are increasingly regarded as functional foods because they contain a rich amount of essential macro- and micronutrients, including proteins, carbohydrates, vitamins, and minerals, along with bioactive compounds, such as tannins, polyphenols, and anthocyanins. Its nutritional composition, antioxidant activity, and several health benefits, including the reduction of oxidative stress and inflammation, improvement in cardiovascular health, and possible anti-cancer effects, are comprehensively discussed in this review. Pomegranates have been widely utilized in the development of functional foods, such as juices, extracts, dairy products, baked goods, and confections. However, the application of pomegranates in products has disadvantages, such as cost ineffectiveness, lack of sensory appeal, and instability of bioactivity. This study further covers consumer acceptance, market trends, and emerging technologies in the development of pomegranate products.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70163","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148048130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenome- and Genome-Wide Insights Into Dietary Patterns: Health Consequences and Genetic Basis 饮食模式的表型和全基因组洞察：健康后果和遗传基础

IF 5.7

eFood Pub Date : 2026-05-13 DOI: 10.1002/efd2.70159

Shuxiao Shi, Ying Dong, Zixin Hu, Libing Shen, Sujing Wang, Shuo Jiang, Deshan Wu, Nannan Feng, Lan Xu, Kexin Li, Kaixiong Ye, Xihao Du, Victor W. Zhong, Jingqi Zhou

{"title":"Phenome- and Genome-Wide Insights Into Dietary Patterns: Health Consequences and Genetic Basis","authors":"Shuxiao Shi, Ying Dong, Zixin Hu, Libing Shen, Sujing Wang, Shuo Jiang, Deshan Wu, Nannan Feng, Lan Xu, Kexin Li, Kaixiong Ye, Xihao Du, Victor W. Zhong, Jingqi Zhou","doi":"10.1002/efd2.70159","DOIUrl":"10.1002/efd2.70159","url":null,"abstract":"Dietary patterns (DPs) integrate complex eating behaviors and provide stronger insights into health than analyses of single nutrients. However, most studies have examined only a limited range of outcomes, and the biological mechanisms underlying DPs remain poorly defined. Using a robust data-driven approach, we derived three major DPs from dietary recall data and evaluated their associations with > 1000 diseases and biomarkers. Mediation analyses investigated metabolic intermediates, and genome-wide association studies (GWAS) identified genetic loci shaping DPs. DP1 represented a plant-forward, nutrient-dense profile; DP2 was meat-and-vegetables oriented; and DP3 was characterized by energy-dense snack intake. In phenome-wide association analyses, DP1 was broadly protective, inversely associated with over 50 diseases, whereas DP3 was detrimental, linked to increased risk across more than 60 outcomes. DP2 showed limited associations. Mediation analyses indicated that the benefits of DP1 were partly attributable to higher circulating polyunsaturated fatty acids, while the harms of DP3 were mediated through lower high-density lipoprotein levels. GWAS revealed distinct genetic underpinnings, linking DP1 to APOE, APOC1, and NEGR1, and DP3 to the MAPT locus. Together, these findings provide a comprehensive characterization of DPs, reveal metabolic and genetic pathways underlying diet-disease relationships, and highlight opportunities for advancing personalized nutrition and disease prevention.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148048031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in High-Throughput Sequencing: Antimicrobial Resistance and Traceability of Foodborne Pathogens 高通量测序研究进展：食源性病原菌的耐药性和可追溯性

IF 5.7

eFood Pub Date : 2026-05-13 DOI: 10.1002/efd2.70158

Kexuan Wu, Jie Zhao, Ningxin Huang, Zhengxiao Wang, Yiping Xu, Shaoning Liu, Chuanlin Bi, Juan Tang, Wei Wu

引用次数: 0

Aspartame Increases the Risk of Pancreatic Ductal Adenocarcinoma 阿斯巴甜增加胰腺导管腺癌的风险

IF 5.7

eFood Pub Date : 2026-05-12 DOI: 10.1002/efd2.70160

Jumin Xie, Wei Fang, Zean Song, Zhang Yu, Ying Zhu, Shaoping Liu

{"title":"Aspartame Increases the Risk of Pancreatic Ductal Adenocarcinoma","authors":"Jumin Xie, Wei Fang, Zean Song, Zhang Yu, Ying Zhu, Shaoping Liu","doi":"10.1002/efd2.70160","DOIUrl":"10.1002/efd2.70160","url":null,"abstract":"Aspartame (APM) is a widely used artificial sweetener associated with various health concerns, including potential links to diabetes, cardiovascular diseases, and an increased risk of cancer. This study aims to investigate the association between APM and cancer risk. In total, 212 key genes were identified from the APM and pancreatic ductal adenocarcinoma (PDAC) targets, univariate Cox analysis across five cohorts, and single-cell sequencing data of malignant cell populations. Twenty-one significant targets were identified through the STRING database. Following machine learning analysis, eight significant targets were highlighted. GO analysis indicated that these core targets were involved in the positive regulation of fibroblast proliferation, stimulus-specific miRNA transcription, and RNA polymerase II transcription repression. KEGG analysis suggested that the core targets were implicated in proteoglycans in cancer, the PI3K-Akt signaling pathway, and microRNAs in cancer. Validation through HPA and immunoinfiltration analyses confirmed the relevance of the eight core targets. Ultimately, FN1, THBS1, and ITGB3 were identified as critical targets through pseudo-time and cell-cell interaction analyses, along with molecular docking and molecular dynamics simulation validation. This study demonstrates that APM consumption is associated with an increased risk of PDAC through FN1, THBS1, and ITGB3 targets, as revealed by integrative analysis.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148048158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoshell Fabrication Techniques for Probiotic Encapsulation: A Comprehensive Review 益生菌包封纳米壳制备技术综述

IF 5.7

eFood Pub Date : 2026-05-04 DOI: 10.1002/efd2.70153

Tuba Mustafa, Muhammad Afzaal, Farhan Saeed, Amna Shahbaz, Zainab Hira, Ayesha Adil, Tayyaba Sohail, Faiyaz Ahmed, Maryam Amanullah, Hina Sarwar, Noor Akram, Saad Mansoor, Gebremichael Gebremedhin Hailu

{"title":"Nanoshell Fabrication Techniques for Probiotic Encapsulation: A Comprehensive Review","authors":"Tuba Mustafa, Muhammad Afzaal, Farhan Saeed, Amna Shahbaz, Zainab Hira, Ayesha Adil, Tayyaba Sohail, Faiyaz Ahmed, Maryam Amanullah, Hina Sarwar, Noor Akram, Saad Mansoor, Gebremichael Gebremedhin Hailu","doi":"10.1002/efd2.70153","DOIUrl":"10.1002/efd2.70153","url":null,"abstract":"Probiotics, beneficial microorganisms, confer numerous health benefits when administered. However, their efficacy is hindered by harsh gut conditions, including acidity, bile acids, and enzymatic degradation. To overcome these challenges, encapsulation and nanoshell technology have emerged as a pivotal strategy to enhance probiotic stability, bioavailability, and specificity. Wall materials comprising proteins, carbohydrates, and lipids protect sensitive core materials like probiotics. In recent advancements, surface-engineered probiotics and nanoshell techniques have demonstrated the ability to regulate development and differentiation. Despite promising outcomes, critical challenges persist in nanoshell fabrication, including production scalability, controlled release behavior, and interaction between encapsulating material and probiotic surfaces. The research is important because it contributes to advancing functional food, next-generation probiotics, and therapeutic delivery systems, which may help treat gastrointestinal tract and health issues. Furthermore, it addresses important knowledge gaps regarding the interaction between encapsulating material and the probiotics' surface. It also discussed future prospects for improving delivery efficiency by adding a functional molecule to enhance colonization. This review aims to provide an up-to-date overview of popular and recent coating materials/biopolymers for probiotic encapsulation. It further explores recent advances in nanoshell production mediated by individual bacteria, and discusses their future prospect for improving delivery efficiency.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of Apoptosis in MCF-7 Breast Cancer Cells by Selenium Nanoparticles Synthesized With Laccase Enzyme Isolated From Pleurotus Floridanus 用佛罗里达侧耳漆酶合成硒纳米颗粒诱导MCF-7乳腺癌细胞凋亡的研究

IF 5.7

eFood Pub Date : 2026-04-28 DOI: 10.1002/efd2.70154

Shenbhagaraman Ramalingam, Arthi Karunanithi, Ammar B. Altemimi, Jenefar Sudarson, Sakthivel Muthu, Kodiganti Naresh Kumar, Rawaa H. Tlay, Tarek Gamal Abedelmaksoud

{"title":"Induction of Apoptosis in MCF-7 Breast Cancer Cells by Selenium Nanoparticles Synthesized With Laccase Enzyme Isolated From Pleurotus Floridanus","authors":"Shenbhagaraman Ramalingam, Arthi Karunanithi, Ammar B. Altemimi, Jenefar Sudarson, Sakthivel Muthu, Kodiganti Naresh Kumar, Rawaa H. Tlay, Tarek Gamal Abedelmaksoud","doi":"10.1002/efd2.70154","DOIUrl":"10.1002/efd2.70154","url":null,"abstract":"This study aimed to develop an eco-friendly and efficient method for synthesizing selenium nanoparticles using purified laccase from Pleurotus floridanus. SeNPs were mycosynthesized at varying sodium selenite concentrations (5–20 mM). Dynamic light scattering analysis revealed particle sizes ranging from 30 to 80 nm, with a mean size of 54 nm and a polydispersity index of 0.843. SEM and HR-TEM images showed predominantly spherical nanoparticles with an average size of 25 nm. Antibacterial assays demonstrated dose-dependent inhibition against Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae, with the highest activity observed against P. aeruginosa at 20 mg/mL concentration. The antiproliferative effect on MCF-7 breast cancer cells was also dose-dependent, with an IC₅₀ of 222 μg/mL and up to 81.2% reduction in viability at 500 μg/mL. Western blot analysis showed marked upregulation of proapoptotic proteins Bax, Caspase 3, Caspase 9, and p53. These results highlight the potent antimicrobial and anticancer properties of SeNPs.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70154","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148040006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Edulitin 2, a Ribotoxin-Like Protein From Boletus edulis That Triggers Apoptosis in Colon Cancer Cells While Preserving the Integrity of the Intestinal Microbiota Edulitin 2，一种来自Boletus edulis的核糖素样蛋白，可触发结肠癌细胞凋亡，同时保持肠道微生物群的完整性

IF 5.7

eFood Pub Date : 2026-04-23 DOI: 10.1002/efd2.70138

Massimo Bortolotti, Sara Ragucci, Federica Falà, Anella Saggese, Francesco Biscotti, Ylenia De Luca, Nicola Landi, Paolo V. Pedone, Andrea Bolognesi, Ezio Ricca, Letizia Polito, Antimo Di Maro

{"title":"Edulitin 2, a Ribotoxin-Like Protein From Boletus edulis That Triggers Apoptosis in Colon Cancer Cells While Preserving the Integrity of the Intestinal Microbiota","authors":"Massimo Bortolotti, Sara Ragucci, Federica Falà, Anella Saggese, Francesco Biscotti, Ylenia De Luca, Nicola Landi, Paolo V. Pedone, Andrea Bolognesi, Ezio Ricca, Letizia Polito, Antimo Di Maro","doi":"10.1002/efd2.70138","DOIUrl":"10.1002/efd2.70138","url":null,"abstract":"Porcini (Boletus edulis Bull.) are a reservoir of pharmacological biomolecules with health-promoting properties. Here, we investigated the cytotoxic activity of edulitin 2, a ribotoxin-like protein purified from B. edulis fruiting bodies, against two human colon cancer cell lines (Caco-2 and HT29), and assessed its potential impact on gut microbiota. Edulitin 2 exhibited marked cytotoxicity toward both cell lines in a dose- and time-dependent manner. The effect was particularly evident after 72-h, with EC50 values in the 10−7 M range for both cell lines. Moreover, edulitin 2 significantly affected barrier integrity in Caco-2 monolayer models. Edulitin 2 kills cells mainly by triggering apoptotic mechanisms, with limited involvement of necroptosis and absence of necrosis. These results support edulitin 2 as a potential therapeutic agent due to the reduced risk of inducing inflammation-related damage. Furthermore, this toxin proved to be harmless to microbial consortia simulating the gut microbiota. Overall, these findings make edulitin 2 a promising bioactive compound that combines cytotoxicity toward colorectal tumor cells with microbiota compatibility. This dual profile supports its further exploration in preclinical models (for loco-regional treatment and as payload of immunotoxins) and highlights the potential of edible mushrooms as a source of novel ribotoxin-like proteins with biomedical relevance.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postbiotic Supernatants From Psychobiotic Strains Modulate Antioxidant and Serotonergic Responses 精神菌菌株的生后清液调节抗氧化和血清素能反应

IF 5.7

eFood Pub Date : 2026-04-23 DOI: 10.1002/efd2.70151

Cocean Ana-Maria, Zorita Diaconeasa, Calina Ciont, Razvan Odocheanu, Alexandra Machitescu, Floricuta Ranga, Dan Cristian Vodnar

{"title":"Postbiotic Supernatants From Psychobiotic Strains Modulate Antioxidant and Serotonergic Responses","authors":"Cocean Ana-Maria, Zorita Diaconeasa, Calina Ciont, Razvan Odocheanu, Alexandra Machitescu, Floricuta Ranga, Dan Cristian Vodnar","doi":"10.1002/efd2.70151","DOIUrl":"10.1002/efd2.70151","url":null,"abstract":"Psychobiotics have gained interest for their role in gut–brain communication through the production of short-chain fatty acids (SCFAs). This study evaluated the postbiotic potential by analyzing the SCFA profile, antioxidant capacity, and serotonergic effects of cell-free supernatant (CFS) obtained from Lactiplantibacillus plantarum 299V and Limosilactobacillus fermentum LC40. During controlled fermentation, the strains accumulated SCFAs, with L. fermentum producing higher levels of butyrate and propionate, whereas L. plantarum reached acetate levels comparable to those of L. fermentum LC40. CFS showed strong antioxidant capacity, characterized by a test-specific profile: CUPRAC (1664.76 and 1626.67 µM TE), ABTS (440.31 and 438.77 µM TE), and DPPH (164.00 and 168.35 µM TE) for L. fermentum and L. plantarum. Cellular assays using RIN-14B enterochromaffin-like cells revealed strain-specific responses: L. fermentum CFS maintained cell viability at low concentrations (10–25 µM butyrate-equivalent) and promoted serotonin release at moderate doses (50–100 µM); in contrast, L. plantarum required higher butyrate-equivalent levels (100–200 µM) for maximal serotonergic activity but showed a slight decrease in viability. These findings underscore the complementary antioxidant and serotonergic potential of psychobiotic-derived CFS, reflecting the integrated activity of SCFAs in a complex postbiotic matrix, supporting their application in functional foods targeting gut and brain health.","PeriodicalId":11436,"journal":{"name":"eFood","volume":"7 3","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/efd2.70151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148039861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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