Current Eye Research最新文献

{"title":"Schirmer Strips in Tear Metabolomics of Dry Eye Disease: Pre-analytical and Analytical Considerations.","authors":"Mazyar Yazdani, Katja Benedikte Prestø Elgstøen, Tor Paaske Utheim","doi":"10.1080/02713683.2025.2596213","DOIUrl":"10.1080/02713683.2025.2596213","url":null,"abstract":"<p><strong>Purpose: </strong>Dry eye disease (DED) is a prevalent ocular surface condition characterized by tear film instability and inflammation, significantly impacting patients' quality of life. While the Schirmer test remains a key diagnostic tool, Schirmer strips are increasingly used for tear fluid collection in metabolomics studies aimed at, e.g. identifying biomarkers for DED. However, both pre-analytical and analytical variables inherent to Schirmer strip use can compromise data integrity and hamper reproducibility.</p><p><strong>Methods: </strong>For this review, the PubMed database and Google Scholar were searched for published human studies in English relevant to pre-analytical and analytical aspects of tear metabolomics, especially from dry eye patients, when tear samples were collected with Schirmer strips.</p><p><strong>Results: </strong>This article discusses the critical pre-analytical factors, including variability in Schirmer strip materials, tear sampling techniques, storage conditions, and potential contamination, that influence metabolomic data quality. Analytical considerations are also examined, with particular focus on volume-adjusted extraction protocols that address tear volume variability.</p><p><strong>Conclusion: </strong>We conclude that standardized guidelines for Schirmer strip use, covering material selection, sampling protocols, storage, and extraction, are urgently needed to advance tear metabolomics as a reliable clinical and research tool. Future research should focus on validating these recommendations in multi-center studies, exploring inter-individual variability, and developing innovative analytical workflows to optimize metabolite recovery and quantification. Such efforts will enhance the translational potential of tear metabolomics, paving the way for biomarker discovery and improved management of DED.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1166-1173"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145667399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Evaluation of TeaRx^™: A Point of Care Multi-Parameter Tear Film Test for Diagnosis, Stratification, and Prediction of Responsiveness to Cyclosporine A Therapy in Dry Eye Disease.","authors":"Sayan Basu, Shimon Gross, Amos Sommer, Avital Beck","doi":"10.1080/02713683.2026.2678293","DOIUrl":"10.1080/02713683.2026.2678293","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate TeaRx^™, a 10-minute, multi-parametric tear test, as a single point-of-care diagnostic device for assessment of dry eye disease (DED), stratifying severity, identifying severe meibomian gland dysfunction (MGD), and predicting patient response to topical cyclosporine A (CysA) therapy.</p><p><strong>Methods: </strong>A single center, observational, longitudinal clinical study enrolled 593 participants graded by TFOS DEWS II criteria: 495 DED (including 83 with severe MGD), and 98 healthy controls. Tear fluid was collected using a microfluidic tear collection device and analyzed for five biomarkers (lactoferrin, human serum albumin, lysozyme, mucin and IgA) by lateral-flow immunochromatographic assay (LFIA). Optimal logistic regression models were selected for differentiating DED vs. healthy, severe vs. non-severe DED, severe MGD vs. non-MGD in DED patients, and prediction (at baseline) of CysA responsiveness.</p><p><strong>Results: </strong>Based on the models selected, TeaRx^™ differentiated DED (at all severity levels) from healthy controls with 71.5% sensitivity, 63.1% specificity, and AUC = 0.72. Severe DED was differentiated from non-severe DED with 80.6% sensitivity, 66.7% specificity, AUC = 0.77. Severe MGD within DED was identified with 80.6% sensitivity, 61.3% specificity, AUC = 0.80. TeaRx^™ predicted CysA responsiveness with 93.8% sensitivity, 63.1% specificity, AUC = 0.86, and NPV = 92.3%.</p><p><strong>Conclusions: </strong>Based on the results of the current study, TeaRx^™ may potentially offer the means for rapid, noninvasive tear biomarker profiling within 10 minutes, providing accurate diagnosis of DED, severity stratification, detection of severe MGD, and prediction of therapeutic response to CysA. These results support its role as a personalized diagnostic platform for optimizing management of DED.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1237-1244"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148041415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tear Film Lipid Layer Thickness: Measurement Techniques, Normative Values and Alteration in Ocular Surface Diseases.","authors":"Sandhya Esam, Swati Singh, Nagaraju Konda, Rashmin Gandhi, Geeta K Vemuganti","doi":"10.1080/02713683.2025.2458735","DOIUrl":"10.1080/02713683.2025.2458735","url":null,"abstract":"<p><strong>Purpose: </strong>Tear film instability is one of the principal pathomechanisms of dry eye disease (DED), arising from disrupted tear homeostasis, reduced tear volume, or lipid layer alterations. The lipid layer, secreted by the meibomian glands, can be imaged qualitatively and quantitatively. This review summarizes different lipid layer thickness (LLT) measurement techniques, normative values, and alterations in ocular surface disorders.</p><p><strong>Method: </strong>Full-texts of 88 of 236 searched articles from PubMed, ScienceDirect, and Google Scholar using the terms 'Tear film lipid layer thickness,' 'Lipid layer thickness', 'Tear film lipids,' 'Dry eye disease,' 'Meibomian gland dysfunction,' 'Meibography,' and 'Interferometry' were reviewed.</p><p><strong>Result: </strong>Advanced imaging techniques, such as LipiView, Oculus Keratograph 5M, and Tearscope, utilize interference principles, but are not interchangeable for measuring LLT. LLT ranges from 13 to 170 nm in healthy individuals, depending on the instrument and grading scale used. The reported central LLT values in DED vary from 33 to 84 nm, varying with disease severity. There are conflicting reports on the association between age, gender, and LLT values. LLT positively correlates with expressible meibomian glands, and negatively with gland loss. LLT has been associated with tear film breakup time, MGD severity, and blink pattern irregularities.</p><p><strong>Conclusion: </strong>The significant overlap in LLT values between the normal individuals and DED patients precludes its role in the diagnosis of DED. Future studies exploring LLT variations under different environmental conditions and across larger cohorts with varying DED severity are essential to enhance clinical applicability.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1199-1213"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corneal Sensitivity, Sub-Basal Corneal Nerve Plexus, and Tear Neuropeptides in Symptomatic Versus Asymptomatic Visual Display Terminal Users.","authors":"Moumi Maity, Mohammad Gufran Siddiqui, Vivek Singh, Sayan Basu, Swati Singh","doi":"10.1080/02713683.2026.2691748","DOIUrl":"10.1080/02713683.2026.2691748","url":null,"abstract":"<p><strong>Purpose: </strong>The current study evaluated the structure and function of corneal nerves in symptomatic versus asymptomatic visual display terminal (VDT) users, along with tear neuropeptides.</p><p><strong>Methods: </strong>A cross-sectional, single-masked study divided VDT users into symptomatic (<i>n</i> = 51; ocular surface disease index (OSDI) > 13, tear osmolarity, noninvasive tear break-up time (NIBUT) < 10 s, and positive corneal staining) and asymptomatic groups (<i>n</i> = 21, OSDI ≤ 13, NIBUT > 10 s). Participants underwent detailed tear film examination (Oculus keratograph 5M), blink rate, corneal sensitivity (Cochet-Bonnet aesthesiometer), and <i>in vivo</i> confocal microscopy (IVCM, HRT II-RCM) testing. ELISA of tear cytokines (IL1a, TNFa, IL6, MMP 9) and neuropeptides (CGRP, NPFF, VIP, Sub P, NGF) were compared between groups.</p><p><strong>Results: </strong>Symptomatic VDT users had more years of VDT use (<i>P</i> = 0.0002), reduced corneal sensitivity (<i>P</i> = 0.02), lower Schirmer I (<i>P</i> < 0.0001), lower blink rate (<i>P</i> < 0.0001), and more partial glands (<i>P</i> < 0.001) than asymptomatic users. The daily hours of VDT use (6.4 vs. 6.2), tear osmolarity, immature and mature dendritic cell densities, and IVCM parameters were similar across the two groups. A weak negative correlation of corneal sensitivity was noted with OSDI (<i>P</i> = 0.03) and blink rate (<i>P</i> = 0.037). Mature dendritic cell density had a positive correlation (<i>P</i> = 0.009) with corneal sensitivity. Symptomatic users showed reduced NGF and elevated MMP-9 and IL-1α compared to asymptomatic VDT users.</p><p><strong>Conclusion: </strong>Symptomatic VDT users have reduced mechanical corneal sensitivity without morphological changes in the sub-basal corneal nerve structure. Reduced NGF and elevated tear IL1a and MMP-9 in symptomatic VDT users indicate ocular surface inflammation in VDT-associated dry eye disease.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1253-1259"},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148301238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SRC Promotes High Glucose-Induced Retinal Endothelial Dysfunction by Suppressing FUNDC1-Mediated Mitophagy in Diabetic Retinopathy.","authors":"Songling Du, Zhenyu Yao","doi":"10.1080/02713683.2026.2727289","DOIUrl":"https://doi.org/10.1080/02713683.2026.2727289","url":null,"abstract":"<p><strong>Purpose: </strong>Diabetic retinopathy (DR) is a leading cause of blindness, yet the role of mitophagy in its pathogenesis remains unclear. This study aims to dissect mitophagy heterogeneity in DR at single-cell resolution and identify key regulators.</p><p><strong>Methods: </strong>Bulk RNA-seq datasets were first used to screen mitophagy-related genes associated with DR, followed by single-cell RNA-seq analysis to define cell-type-specific mitophagy alterations. Mitophagy activity was scored by four methods (AddModuleScore, AUCell, ssGSEA, and PercentageFeatureSet). Cell communication and pseudotime trajectories were inferred with CellChat and Monocle3. The function of SRC in high glucose-induced human retinal endothelial cells (HRECs) was validated by RT-qPCR, Western blot, CCK-8, Transwell, and transmission electron microscopy.</p><p><strong>Results: </strong>Endothelial cells exhibited the highest mitophagy activity in DR. Immature endothelial cells showed the highest mitophagy scores and occupied a key transition state in differentiation trajectory. SRC was consistently upregulated in DR and its knockdown restored mitophagy by promoting FUNDC1-LC3 interaction, thereby suppressing high glucose-induced proliferation and migration of HRECs.</p><p><strong>Conclusion: </strong>This study reveals cell-specific mitophagy dysregulation in DR and identifies SRC as a critical regulator of FUNDC1-mediated mitophagy. Targeting SRC may offer a novel therapeutic strategy for DR.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complications and Safety Profile of XEN^® Gel Stent in Glaucoma: A Five-Year MAUDE Database Analysis.","authors":"Alina Ghazou, Yousef Hawas, Martin W Gerges, Aya Awad, Ibraheem M Alkhawaldeh, Mohamed Marouf, Asem A Alqudah","doi":"10.1080/02713683.2026.2728913","DOIUrl":"https://doi.org/10.1080/02713683.2026.2728913","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze adverse events associated with the XEN^® Gel Stent using the FDA's Manufacturer and User Facility Device Experience (MAUDE) database.</p><p><strong>Methods: </strong>The FDA MAUDE database was queried for reports from January 1, 2020, to November 8, 2024, using the product code \"KYF.\" Reports unrelated to the XEN^® Gel Stent were excluded. Data were analyzed for patient demographics, event types, and device-related complications. Descriptive statistics were used to outline the trends in device-related and patient-related issues.</p><p><strong>Results: </strong>A total of 1,816 reports were identified. After excluding 688 reports unrelated to the XEN^® Gel Stent, 338 reports that originated from the literature rather than primary MAUDE submissions, and 2 reports with insufficient information, 788 reports were included for analysis. The most frequently reported device-related issues included Difficult to insert (device sticking) (<i>n</i> = 217, 36.7%), Obstruction of flow (<i>n</i> = 78, 13.2%), device malposition (68, 11.5%). Common patient-related complications included increased intraocular pressure (IOP) (142, 21.8%) and eye injury (137, 21.0%), followed by erosion (77, 11.8%).</p><p><strong>Conclusions: </strong>Over five years, the MAUDE database has revealed that the most common issues with the XEN^® Gel Stent involve device-related problems like resistance and blockage, as well as patient complications. While the data comes from voluntary reports and has its limitations, these real-world insights are valuable.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148976022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redistribution of Aquaporin Proteins in Retinal Blood Vessels in Human Diabetic Retinopathy.","authors":"Alexis Ceecee Britten-Jones, Rosica Petrova, Colin R Green, Angus C Grey, Monica L Acosta","doi":"10.1080/02713683.2026.2726019","DOIUrl":"https://doi.org/10.1080/02713683.2026.2726019","url":null,"abstract":"<p><strong>Purpose: </strong>Diabetic macular edema, the primary cause of vision loss in non-proliferative diabetic retinopathy (NPDR), is traditionally attributed to microvascular leakage but may also involve structural and caliber change in large retinal vessels. This study investigated the spatial distributions of key aquaporins from the aquaporin family mediating water transport or membrane stability in donor human retinas with diabetic retinopathy (DR).</p><p><strong>Methods: </strong>Human donor retinas were fixed, sectioned and labeled for immunohistochemistry using antibodies against Aquaporin 0 (AQP0), Aquaporin 1 (AQP1) and Aquaporin 4 (AQP4). Labeling was performed in combination with vascular and cellular markers including calbindin, A kinase anchor protein-2 (AKAP-2), vimentin, glial fibrillary acidic protein (GFAP), Kir4.1, and collagen IV. Images were acquired using high-resolution laser scanning confocal microscopy and analyzed using ImageJ.</p><p><strong>Results: </strong>AQP0 distribution in the inner retina remained unchanged in DR. In contrast, both AQP1 and AQP4 exhibited marked redistribution within and around large retinal vessels. Specifically, AQP1, typically confined to the endothelium of superficial vessels, was detected in the middle layer of vessel walls in DR. AQP4, normally localized to the ganglion cell layer and associated with vimentin-positive Müller glia processes, was observed in the outer vessel layers, even in the absence of significant GFAP upregulation.</p><p><strong>Conclusions: </strong>The novel localization of AQP1 within the vessel wall may reflect altered spatial organization of water transport pathways within retinal vessel walls, contributing to impaired contractile function. These findings indicate that alterations in aquaporin distribution may be associated with vascular remodeling observed in DR.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head vs Screen-Mounted Eye Trackers: Comparisons Across Ethnic Diversity.","authors":"Alice Cade, Jasmine Zoest, Misaki Kameyama, Laura Dukes, Philip R K Turnbull","doi":"10.1080/02713683.2026.2727290","DOIUrl":"https://doi.org/10.1080/02713683.2026.2727290","url":null,"abstract":"<p><strong>Introduction: </strong>Eye tracking accuracy and reliability may be influenced by physiological diversity and eye tracker design, yet systematic biases remain poorly characterized. To test whether features such as iris color, palpebral aperture, or skin type contribute to variability in performance, we conducted a cross-over experiment using two different eye trackers across a diverse range of participants to inform how future work can address equity and device design.</p><p><strong>Methods: </strong>Forty healthy participants were assessed performing identical tasks using two eye trackers: a head-mounted tracker (HMT, Pupil Labs Core) and a screen-mounted tracker (SMT, Tobii Eye Tracker 4 C). Stimuli were presented across various positions of the screen in a controlled environment. Eye tracker performance was compared across participant features (iris color, palpebral aperture, skin type).</p><p><strong>Results: </strong>The SMT performed significantly better with non-brown irises and lighter skin types, and the HMT with brown irises and darker skin types. Overall, the HMT had a higher mean percentage of valid data; however, this came at the expense of reduced accuracy and precision.</p><p><strong>Conclusions: </strong>This study identified variation in eye tracker performance depending on the tracker placement, and participant iris color or skin type. Both trackers exhibited similar gaze errors, but only the SMT showed acceptable precision <i>via</i> test-retest reliability. These data highlight the need for further research to ensure equitable application of eye trackers in diverse populations, and how iris color or skin type might affect gaze error and reliability.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Fushiming Against Microwave Radiation-Induced Retinal Damage: A Network Pharmacology and Experimental Study.","authors":"Yue Chen, Kai Wang, Sicheng Yang, Jinyi Cao, Jingang Ma, Yi Qiao, Lu Lei, Bin Feng, Shuibing Liu, Zhifu Yang","doi":"10.1080/02713683.2026.2724598","DOIUrl":"10.1080/02713683.2026.2724598","url":null,"abstract":"<p><strong>Purpose: </strong>Human health could be at risk from microwave radiation, especially for the eyes because of their unique anatomical and visual characteristics. This study aimed to investigate whether the Fushiming capsule can protect against microwave radiation-induced retinal damage, oxidative stress, and pathological vascular neovascularization.</p><p><strong>Methods: </strong>A network pharmacology approach was adopted to identify the primary components and action targets of Fushiming with potential treatment effects on the retinal diseases. Moreover, we predicted and verified its mechanisms of action.</p><p><strong>Results: </strong>Network pharmacology analysis identified 43 principal components in Fushiming, and the protein-protein interaction network analysis revealed several key targets, such as VEGFA and VCAM-1. GO enrichment analysis showed that oxidative stress was an important biological process involved in the pathophysiology of the retinal diseases. <i>In vivo</i> and <i>in vitro</i> experiments revealed that Fushiming significantly alleviated microwave radiation-induced retinal pathological damage and improved electroretinogram responses to restore retinal function. Furthermore, Fushiming significantly increased the serum concentration of superoxide dismutase, glutathione peroxidase, and catalase activities, while reducing reactive oxygen species accumulation. Fushiming markedly downregulated the expression of VEGF, VEGFA, eNOS, VEGFR2, and MMP2 proteins in tissue and cellular samples.</p><p><strong>Conclusion: </strong>The present results indicate that Fushiming can prevent microwave radiation-induced retinal damage by enhancing antioxidant pathways and inhibiting angiogenesis-related processes.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eriocitrin Alleviates Choroidal Neovascularization by Promoting the Activities of Mitogen-Activated Protein Kinase Phosphatase-1 <i>In Vivo</i> and <i>In Vitro</i>.","authors":"Yue Zan, Xiaona Wang, Xuefeng Xiao, Zhi Jia, Yuting Zhang, Manhui Zhu, Gang Hao","doi":"10.1080/02713683.2026.2728911","DOIUrl":"https://doi.org/10.1080/02713683.2026.2728911","url":null,"abstract":"<p><strong>Purpose: </strong>Choroidal neovascularization causes vision loss, necessitating the development of novel therapeutic methods. This study examines the role of eriocitrin, a flavonoid, in choroidal neovascularization and its potential mechanism of action.</p><p><strong>Methods: </strong>A murine laser-induced choroidal neovascularization model and hypoxia-exposed human choroidal endothelial cells were employed. <i>In vivo</i>, eriocitrin or the anti-vascular endothelial growth factor agent conbercept was administered via intravitreal injection. Fundus fluorescein angiography, indocyanine green angiography, and immunofluorescent staining were used to assess choroidal neovascularization. <i>In vitro</i>, human choroidal endothelial cells were treated with eriocitrin under hypoxic conditions. Cell proliferation, migration, and tube formation were evaluated using 5-ethynyl-2'-deoxyuridine, Transwell, and tube formation assays. Protein levels of mitogen-activated protein kinase phosphatase-1, p-p38, and p-JNK were detected by Western blot.</p><p><strong>Results: </strong>Eriocitrin significantly attenuated choroidal neovascularization development and vascular leakage in mice in a dose-dependent manner, with a 4 µg dose showing efficacy comparable to conbercept. In hypoxic human choroidal endothelial cells, eriocitrin inhibited proliferation, migration, and tube formation. Under hypoxic conditions, eriocitrin elevated mitogen-activated protein kinase phosphatase-1 protein levels and reduced p-p38 and p-JNK expression. Inhibition of mitogen-activated protein kinase phosphatase-1 diminished the suppressive effects of eriocitrin on hypoxia-induced pro-angiogenic activities.</p><p><strong>Conclusion: </strong>Eriocitrin alleviates choroidal neovascularization by promoting mitogen-activated protein kinase phosphatase-1 activity in choroidal endothelial cells, thereby inhibiting the p38 and JNK signaling pathways. These findings suggest eriocitrin as a potential therapeutic agent for choroidal neovascularization.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}