Current Eye Research最新文献

{"title":"Posterior Polar Cataracts: A Comprehensive Review of Management Strategies.","authors":"Manasi Tripathi, Manpreet Kaur, Jeewan Singh Titiyal, Sridevi Nair","doi":"10.1080/02713683.2025.2506526","DOIUrl":"https://doi.org/10.1080/02713683.2025.2506526","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the recent updates on etiopathogenesis, clinical features, imaging modalities, and management strategies for challenging posterior polar cataracts.</p><p><strong>Methods: </strong>A thorough review was conducted by systematically analyzing the literature published on posterior polar cataracts, including its etiopathogenesis, clinical presentation, imaging modalities, surgical techniques, newer innovations and postoperative outcomes. The comprehensive search was performed on three databases (PubMed, Scopus, and Google Scholar) using relevant keywords such as \"posterior polar cataract,\" \"ASOCT\", \"UBM\", \"femtosecond laser,\" \"phacoemulsification\" and \"complications.\" Articles published in English were included, with priority given to peer-reviewed studies, clinical trials, and systematic reviews. Additional references were identified through cross-referencing key publications. The gathered data were synthesized to provide an overview of current knowledge and evolving perspectives in the management of posterior polar cataracts.</p><p><strong>Results: </strong>This mini-review comprehensively analyses and subsequently highlights the various clinical signs and imaging peculiarities seen on different investigative modalities to identify posterior polar cataracts. The information thus gathered will immensely help surgeons to effectively perform risk stratification, formulate the appropriate surgical plan, and manage patient expectations. Numerous surgical techniques have also been identified and extensively discussed to manage posterior polar cataracts in different scenarios.</p><p><strong>Conclusion: </strong>This mini-review serves as a comprehensive guide for both trainee and practicing ophthalmologists alike, updating them on recent advances in posterior polar cataracts. It also highlights the inherent challenges and key considerations that clinicians must carefully navigate in this evolving field.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Inter-Device Agreement in Quantifying Choriocapillaris Flow Deficits in Healthy Eyes.","authors":"Giacomo Boscia, Ramya Gnanaraj, Giulia Corradetti, Ceren Soylu, Shin Kadomoto, Yongsok Ji, Wongsiri Taweebanjongsin, Li Su, Iksoo Byon, Srinivas R Sadda","doi":"10.1080/02713683.2025.2506524","DOIUrl":"https://doi.org/10.1080/02713683.2025.2506524","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the computation of the choriocapillaris flow deficit obtained from three different optical coherence tomography angiography devices in healthy subjects.</p><p><strong>Methods: </strong>Twenty-six healthy subjects were included in this post hoc analysis of data from a prospective healthy subject optical coherence tomography angiography study. Fovea-centered 6 × 6 optical coherence tomography angiography volume scans were obtained by all three devices: Heidelberg Spectralis spectral domain optical coherence tomography angiography (Heidelberg, Germany) using a full spectrum probabilistic algorithm (512 × 512 pixels); Zeiss Cirrus 5000 AngioPlex (Zeiss Meditec, Dublin, CA) (spectral domain-optical coherence tomography angiography) using the optical microangiography algorithm (350 × 350 pixels); Zeiss PLEX Elite 9000 swept source optical coherence tomography angiography (Zeiss Meditec, Dublin, CA) using the optical microangiography algorithm (500 × 500 pixels). Choriocapillaris slabs were binarized using Image J (Bethesda, MD) and the choriocapillaris flow deficit % was measured applying a Phansalkar radius of ∼20 µm, adjusted for each device based on the size and resolution of the images.</p><p><strong>Results: </strong>Twenty-six healthy eyes of 26 subjects with no ocular or systemic disease were included in this analysis. The mean age of the subjects was 47.60 ± 13.00 (range, 26-69) years and 46.15% were females. The choriocapillaris flow deficit % measured by the devices varied significantly from each other (<i>p</i> < .01). The mean choriocapillaris flow deficit % measured by the Spectralis (Heidelberg, Germany) was the highest at 57.00% (±13.21%), followed by the Angioplex at 25.70% (±9.00%), and the Plex Elite at 7.62 (±3.06%). There was a significant positive correlation between choriocapillaris flow deficit % measured by swept source-optical coherence tomography angiography and spectral domain-optical coherence tomography angiography devices (Angioplex and PLEX Elite) using the optical microangiography algorithm (<i>r</i> = 0.78, <i>p</i> < .01). Of note, this correlation was better than the correlation between two spectral domain-optical coherence tomography angiography devices using different algorithms (Spectralis, Heidelberg, Germany, full spectrum probabilistic algorithm and Angioplex optical microangiography) (<i>r</i> = 0.389, <i>p</i> = .04).</p><p><strong>Conclusion: </strong>The correlation between swept source and spectral domain optical coherence tomography angiography devices using the same optical coherence tomography angiography algorithm is better than between spectral domain-optical coherence tomography angiography devices using different algorithms.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Refractive Parameter and Serum Vascular Endothelial Growth Factor Concentration and Optical Coherence Tomographic Angiography in Myopia Children with Retinopathy of Prematurity.","authors":"Chen Chen, Liu Hong, Xiang Ma","doi":"10.1080/02713683.2025.2503202","DOIUrl":"https://doi.org/10.1080/02713683.2025.2503202","url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to investigate the risk factors contributing to myopia in children after the spontaneous regression of retinopathy of prematurity (ROP), focusing on the relationship between serum vascular endothelial growth factor (VEGF) concentration, refractive parameters, and optical coherence tomographic angiography (OCTA) findings.</p><p><strong>Methods: </strong>A prospective observational cohort of 198 patients who underwent fundus examinations at our hospital from January 2020 to December 2022, including 50 with myopia after regressed ROP (ROP-myopia group), 48 without myopia after regressed ROP (ROP-non-myopia group), 51 healthy myopic children (Myopia group), and 49 healthy non-myopic children (non-Myopia group), were enrolled. Serum VEGF levels, refractive parameters, and OCTA parameters were measured. Statistical analyses, including logistic regression, were performed to identify correlations between VEGF levels, OCTA parameters, and refractive outcomes.</p><p><strong>Results: </strong>The ROP-myopia group had higher diopter (3.66 ± 0.83 D), lens thickness (4.91 ± 0.91 mm), and axial length (24.30 ± 1.58 mm), along with elevated serum VEGF levels (302.8 ± 14.00 pg/ml), compared to other groups (<i>p</i> < 0.05). Retinal vascular density (VD) and central foveal thickness (CFT) was higher in the ROP-myopia group (<i>p</i> < 0.05). VEGF, VD, and CFT were identified as independent risk factors for abnormal refractive parameters, with an ROC curve area of 0.9918 (<i>p</i> < 0.05), highlighting their significant roles in myopia development after regressed ROP.</p><p><strong>Conclusion: </strong>The study highlights the significant influence of VEGF, VD, and CFT on refractive outcomes in children with regressed ROP. Monitoring changes in these parameters is crucial for early intervention to reduce the risk of myopia development.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Objective Quality Assessment of the Capsulorhexis - A Novel Augmented Reality Tool for Trainees and Experts.","authors":"Daniel Rudolf Muth, Pauline Scherm, Federico Acosta, Miroslav Brêzik, Fernando Benito, Martin Dirisamer, Siegfried Georg Priglinger, Frank Blaser, Sandrine Anne Zweifel, Wolfgang Johann Mayer","doi":"10.1080/02713683.2025.2504569","DOIUrl":"10.1080/02713683.2025.2504569","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the usability of a smartphone-based augmented reality tool for capsulorhexis training.</p><p><strong>Methods: </strong>This is a single-center, prospective, clinical study conducted at the Department of Ophthalmology, University Hospital, LMU Munich, Munich, Germany. Surgery-naïve residents were included and divided into three subgroups: (1) augmented reality supported by the Custom Surgical MicroREC system during their capsulorhexis procedures; (2) Expert support supported by an experienced cataract surgeon; (3) No support without support. Capsulorhexis quality was graded by circularity, centration, and radius.</p><p><strong>Results: </strong>Twenty-one (<i>n</i>_total = 21) participants were enrolled. The augmented reality group was statistically significantly better in terms of circularity than the Expert support (Mann-Whitney's <i>U</i>-test: <i>p</i> = .00175) and the No support group (Mann-Whitney's <i>U</i>-test: <i>p</i> = .00268). The procedure duration decreased statistically significantly for all three groups when try 1 and 10 were compared (augmented reality: <i>p</i> < .0001; Expert support: <i>p</i> = .0099; No support: <i>p</i> < .0001). The other parameters did not differ statistically significantly. The questionnaires handed to the augmented reality and No support group before and after the wet lab revealed a high importance of wet lab trainings to all polled participants. The provided wet lab setup was considered adequate by both groups.</p><p><strong>Conclusion: </strong>Augmented reality can assist in cataract training. The Custom Surgical MicroREC system can have a positive influence on capsulorhexis quality in a training environment. Automated quality metrics measured by the augmented reality software provide an objective quality score of the performed surgical procedure. As the tested augmented reality system can be used in training as well as in a real-world setting, this will be a useful tool for trainees and expert surgeons alike.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"circ_EPB41 Modulates the miR-15a-5p/Bcl-2 Pathway to Alleviate Oxidative Stress Damage in Lens Epithelial Cells.","authors":"Jie Fu, Shiyao Huang, Shaodan Hu, Lifen Huang, Huanjie Fang","doi":"10.1080/02713683.2025.2506116","DOIUrl":"https://doi.org/10.1080/02713683.2025.2506116","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the role of circ_EPB41 in regulating oxidative stress-induced apoptosis in lens epithelial cells by targeting the miR-15a-5p/Bcl-2 pathway, highlighting its potential significance in age-related cataract treatment. Growing evidence suggests that circular RNAs and microRNAs play critical roles in age-related cataract development, making circ_EPB41 a promising target.</p><p><strong>Methods: </strong>SRA01/04 cells were exposed to 200 µM H₂O₂ for 24 h to induce oxidative damage and model age-related cataract. Cell viability and apoptosis were assessed using MTT assays and flow cytometry, respectively. The interaction between circ_EPB41 and miR-15a-5p was analyzed through bioinformatics tools and dual luciferase reporter assays. Additionally, the expression levels of Bax, Bcl-2, miR-15a-5p, and circ_EPB41 were measured using RT-qPCR and Western blot analysis.</p><p><strong>Results: </strong>H₂O₂-induced SRA01/04 cells showed down-regulation of circ_EPB41 and up-regulation of miR-15a-5p. Circ_EPB41 was found to interact with miR-15a-5p, negatively regulating its expression. Furthermore, miR-15a-5p mimics reversed the effects of circ_EPB41 on oxidative stress in SRA01/04 cells, as evidenced by reduced cell viability, increased apoptosis, elevated Bax levels, and decreased Bcl-2 expression. In contrast, miR-15a-5p inhibitors alleviated H₂O₂-induced oxidative damage by upregulating Bcl-2 expression.</p><p><strong>Conclusion: </strong>This study is the first to demonstrate that up-regulation of circ_EPB41 protects lens epithelial cells from oxidative stress-induced apoptosis through the miR-15a-5p/Bcl-2 pathway. These findings suggest that circ_EPB41 may hold potential as a novel therapeutic target for age-related cataract treatment.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles of Tears and Ocular Surface: An Enigma.","authors":"Asra Fatima, Jyothi Attem, Sandhya Esam, Geeta K Vemuganti","doi":"10.1080/02713683.2025.2503214","DOIUrl":"https://doi.org/10.1080/02713683.2025.2503214","url":null,"abstract":"<p><strong>Purpose: </strong>A stable ocular surface is crucial for maintaining ocular health by protecting against various infections. This is achieved by coordinated function of ocular structures (cornea, limbus, conjunctiva), innervation, and the tear film which forms a protective barrier over the ocular surface ensuring proper hydration, lubrication, and overall ocular comfort. This complex three-layered tear film secreted by different sources ensures its stability by adhesion to the corneal epithelium. Ocular surface fluid kinetics and tear secretion involve complex processes influenced by neural regulation, environmental factors, and molecular composition. Recent advances in cell biology and secretome has unravelled the mysteries of cellular cargo of almost every cell and system i.e. the extracellular vesicles (EVs) which facilitate intercellular communication. EVs are of different sizes, amongst which small EVs (sEVs) potentially are more informative than other EVs.</p><p><strong>Methods: </strong>An extensive review of literature on sEVs in tears and ocular surface was conducted.</p><p><strong>Results: </strong>Emerging literature on sEVs derived from ocular surface structures such as cornea and limbal stem cells contribute to corneal wound healing, regeneration and reduced fibrosis by the activation of specific proteins. A recent study documents that homeostasis between cornea and conjunctiva is maintained by the expression of specific genes triggering <i>trans</i> differentiation in diseased conditions. There is also mounting evidence on role of tear-derived sEVs in normal and diseased states. The approach in which tear layers secreted from three different sources form into a single tri-layered stable biofilm covering the entire ocular surface remains elusive. Hence not surprisingly, the tear sEVs therefore have been referred to as one entity and not attributed to any of the 3 different sources that they originate from.</p><p><strong>Conclusion: </strong>This review attempts to present the recent concepts of sEVs, ocular surface, tears and highlight the gaps in our understanding of tear-derived exosomes and its potential role in homeostasis and disease conditions.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-15"},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERPINA3 in Diabetic Retinopathy: A Pilot Study Assessing the Impact of Prior COVID-19 Infection on Patients.","authors":"Arthur Miller, Eunheh Koh, Rebekah Robinson, Vikas Kumar, Ashok Sharma, Shruti Sharma","doi":"10.1080/02713683.2025.2494795","DOIUrl":"10.1080/02713683.2025.2494795","url":null,"abstract":"<p><strong>Purpose: </strong>SERine Protease INhibitor clade A member 3 (SERPINA3), a protease inhibitor involved in the acute phase response, is regulated by interleukin 6 trans-signaling, a pro-inflammatory pathway associated with diabetic retinopathy (DR) pathogenesis. We hypothesized that SERPINA3 may serve as a biomarker to distinguish DR patients from diabetic patients without retinopathy.</p><p><strong>Methods: </strong>We measured serum SERPINA3 levels in DR patients and demographically matched diabetic patients without retinopathy to assess the biomarker potential of SERPINA3. History of COVID-19 infection was included as a demographic variable.</p><p><strong>Results: </strong>Our results showed that SERPINA3 levels did not vary between cases and controls, nor was there any correlation with disease severity as measured by hemoglobin A1c and diabetic retinopathy stage. We found that excluding patients with a history of COVID-19 infection increased the predictive value of SERPINA3, and that SERPINA3 levels were significantly increased in those subjects hospitalized due to COVID-19. The predictive value of SERPINA3 for hospitalization was similar to age, duration of diabetes, and HbA1c in our cohort.</p><p><strong>Conclusions: </strong>Overall, SERPINA3 had poor sensitivity and specificity for identifying DR patients, and COVID-19 infection history significantly increased variance in SERPINA3 levels. Further investigation into the potential of SERPINA3 as DR biomarker should be sufficiently powered to account for the potentially confounding effects of COVID-19 infection.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Haptic Design, Passing Direction, and Inter-Flange Distance on the Position of the Intraocular Lens during 4-Flanged Intrascleral Fixation.","authors":"Jong Hwa Jun, Seung Pil Bang, Kyu Young Shim, Alexander C Lieu, Natalie A Afshari","doi":"10.1080/02713683.2025.2495218","DOIUrl":"https://doi.org/10.1080/02713683.2025.2495218","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to investigate the effect of haptic design, direction of suture passage, and distance between flanges on the haptic-iris distance after four-flanged scleral fixation of a single-piece intraocular lens with four eyelets.</p><p><strong>Methods: </strong>After marking the 3 and 9 o'clock positions on the limbus, each 26-G needle was positioned 2.0 mm posterior to the limbus and passed 2.0, 3.0, and 4.0 mm away from the markings at 3 o'clock and 9 o'clock, respectively. We passed each end of a 5-0 polypropylene suture anteroposteriorly or posteroanteriorly through two unilateral eyelets. The haptic-iris distance was evaluated using anterior-segment optical coherence tomography.</p><p><strong>Results: </strong>Passing the suture through the eyelets in the posteroanterior direction yielded a wider haptic-iris distance than that achieved by passing the suture in the anteroposterior direction (<i>p</i> < 0.001, respectively). A wider distance between the polypropylene flanges correlated with reduced distortion of haptic structures. Fixation of the Akreos AO with a 4.0-mm suture spacing induced a wider haptic-iris distance (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Passing sutures through the eyelet in the posteroanterior direction during intrascleral fixation proved to be more effective in securing a sufficient haptic-iris distance. A wider distance between flanges was beneficial in preventing structural distortion of the haptic and ensuring a proper distance between the haptic and iris.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corneal Epithelial Changes in Eyes with Post-Traumatic Recurrent Corneal Erosion: Comparison with Fellow Eyes and Healthy Controls.","authors":"Ritika Mukhija, Rachana P Shah, Issac Levy, Mayank A Nanavaty","doi":"10.1080/02713683.2025.2503209","DOIUrl":"https://doi.org/10.1080/02713683.2025.2503209","url":null,"abstract":"<p><strong>Purpose: </strong>To assess corneal epithelial thickness (ET) changes in affected and fellow eyes of patients with post-traumatic recurrent corneal erosions (RCE) and compare these with healthy age-matched controls.</p><p><strong>Methods: </strong>This is a single-centre observational study involving retrospective data collection for patients presenting with traumatic RCE. ET maps were obtained at the follow-up visit once the erosion had healed. Data was recorded for affected (Group 1) and fellow eyes (Group 2) and for healthy age-matched control eyes (Group 3). The primary outcome measure was comparison of \"E-Std Dev\" values (variation in ET maps between the three groups). Secondary outcome measures were central ET, minimum ET, maximum ET, superior ET, inferior ET and E(min-max) values, and qualitative map assessment, which was performed by analysing the number of zones with thicknesses 60-70 µ and 70-80 µ.</p><p><strong>Results: </strong>Thirty-three patients (66 eyes) and 33 age-matched healthy eyes were included. E-Std Dev values were highest values in Group 1 and lowest in Group 3 (4.65 ± 1.87 µ, 2.92 ± 1.16 µ and 1.94 ± 0.63 µ in Groups 1, 2 and 3 respectively) (<i>p</i> < 0.01). A similar trend was also noted for central ET (<i>p</i> < 0.01), maximum ET (<i>p</i> < 0.01), E(min-max) values (<i>p</i> = 0.03) and qualitative map assessment (<i>p</i> < 0.01). There were no significant inter-group differences for minimum ET (<i>p</i> = 0.05), superior ET {E-S (2-7)} (<i>p</i> = 0.45) and inferior ET {E-I (2-7)} (<i>p</i> = 0.68).</p><p><strong>Conclusions: </strong>Variation and maximum ET were highest in the affected eyes. Even the fellow eyes showed higher values than healthy controls. This may suggest a predisposition of some patients to develop RCE after trauma.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue Light Irradiation Elicits Senescence of Corneal Endothelial Cells <i>In Vitro</i> by Provoking Energy Crisis, Inflammasome Assembly and DNA Damage.","authors":"Xin Zheng, Guo-Jian Jiang, Ting-Jun Fan","doi":"10.1080/02713683.2025.2497330","DOIUrl":"https://doi.org/10.1080/02713683.2025.2497330","url":null,"abstract":"<p><strong>Purpose: </strong>The blue light from the digital screens endangers the visual system among which the corneas at the outmost of eyes are vulnerable to the irradiation. Therein, the human corneal endothelial (HCE) cells are crucial to maintain corneal transparency and their damage leads to HCE decompensation resulting in blindness ultimately. Thus, understanding the phototoxic effects of the blue light on the HCE cells and the underlying mechanisms is important for taking measures to protect the vision clarity from the blue-light hazard.</p><p><strong>Methods: </strong>We pulse-irradiated the HCE cell line cells at logarithmic phase for 3 passages using 440 nm blue light and examined the levels of reactive oxygen species (ROS), ATP, nicotinamide adenine dinucleotide (NAD⁺) and autophagy using cytochemistry assay to investigate the alterations of energy metabolism. Moreover, we examined the γH2AX⁺ cells using immunofluorescence and expression of poly(ADP-Ribose)polymerase1 (PARP1) using western blotting to investigate the degrees of DNA damage and repair. We also monitored the levels of inflammasome using western blotting and senescence associated secretory phenotypes (SASPs) of interleukin (IL)-8, IL-1β and IL-6 using qPCR and ELISA to investigate the inflammasome assembly and secretion of SASPs. We detected the senescent features with senescence-associated-β-galactosidase assay, p16 levels by western blotting, Lamin B1 localization by immunofluorescence observation, cell growth by EdU incorporation assay and confluence forming time and alterations of the cell morphology and relative areas by microscopy observation.</p><p><strong>Results: </strong>The HCE cells exhibited senescent features after blue-light-pulse-irradiation. The blue light provokes overproduction of ROS to decrease the levels of ATP, NAD⁺ and autophagy leading to energy crisis. Moreover, the excess ROS injure DNA and downregulate PARP1 resulting in stable cell-cycle arrest. The excess ROS also facilitate inflammasome assembly leading to hypersecretion of SASPs.</p><p><strong>Conclusion: </strong>The blue light elicits HCE cell senescence <i>via</i> inducing energy crisis, stable cell-cycle arrest and SASP hypersecretion.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}