Current Eye Research最新文献

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Comparison of Corneal Biomechanical Efficacy Between Rose Bengal-Green Light and Riboflavin-UVA Crosslinking. 玫瑰红-绿光和核黄素-UVA 角膜交联的角膜生物力学功效比较
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-15 DOI: 10.1080/02713683.2024.2353267
Nilufer Yesilirmak, Ozge Saritas
{"title":"Comparison of Corneal Biomechanical Efficacy Between Rose Bengal-Green Light and Riboflavin-UVA Crosslinking.","authors":"Nilufer Yesilirmak, Ozge Saritas","doi":"10.1080/02713683.2024.2353267","DOIUrl":"10.1080/02713683.2024.2353267","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate corneal biomechanical changes after corneal cross-linking (CXL) treatments with rose bengal-green light (RB-CXL) and riboflavin-UVA (RF-CXL).</p><p><strong>Methods: </strong>A total of 60 freshly enucleated lamb eyes were obtained for this experimental study. Fifteen eyes were treated with RB-CXL using 0.1% RB solution (Group 1), 15 eyes were treated with RB-CXL using 0.2% RB solution (Group 2), 15 eyes were treated with RF-CXL using 0.1% RF solution (Group 3), and 15 eyes were used as controls (Group 4). The same treatment protocol (10-minute irradiation using a total of 5.4 J/cm<sup>2</sup> energy) was applied to all treatment groups. To evaluate corneal biomechanical changes, the stress-strain test was used for both the treated and control corneas. The elastic modulus was calculated using the tension strain curves obtained during the test.</p><p><strong>Results: </strong>The average elastic modulus values were calculated to be 18.9, 23.5, 22.3, and 14.1 MPa in Groups 1, 2, 3, and 4, respectively. Statistically significant differences were found between the groups (<i>p</i> < 0.001 for Group 1 vs. 2; <i>p</i> < 0.001 for Group 1 vs. 3; <i>p</i> < 0.001 for Group 1 vs. 4; <i>p</i> = 0.002 for Group 2 vs. 3; <i>p</i> < 0.001 for Group 2 vs. 4; and <i>p</i> < 0.001 for Group 3 vs. 4).</p><p><strong>Conclusions: </strong>In this study, the efficacy of RB-CXL treatment applied using different concentrations of RB solutions at a total energy of 5.4 J/cm<sup>2</sup> was investigated, and 0.2% RB solution was found to have at least as much and even more effective than the RF-CXL (0.1% RF) on the corneal elasticity module. These results are encouraging for the treatment of ectatic corneas particularly below 400 μm. It is considered that the findings obtained from this study will guide future experimental and clinical studies.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"942-948"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liquiritin Alleviates Inflammation in Lipopolysaccharide-Induced Human Corneal Epithelial Cells. 利奎尼丁能缓解脂多糖诱导的人角膜上皮细胞炎症。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-20 DOI: 10.1080/02713683.2024.2353263
Xian He, Ziyang Zhang, Meili Hu, Xinyi Lin, Xu Weng, Jiajun Lu, Li Fang, Xianhua Chen
{"title":"Liquiritin Alleviates Inflammation in Lipopolysaccharide-Induced Human Corneal Epithelial Cells.","authors":"Xian He, Ziyang Zhang, Meili Hu, Xinyi Lin, Xu Weng, Jiajun Lu, Li Fang, Xianhua Chen","doi":"10.1080/02713683.2024.2353263","DOIUrl":"10.1080/02713683.2024.2353263","url":null,"abstract":"<p><strong>Purpose: </strong>This research was designed to elucidate the anti-inflammatory impacts of liquiritin on lipopolysaccharide (LPS)-activated human corneal epithelial cells (HCECs).</p><p><strong>Methods: </strong>The Cell Counting kit-8 (CCK-8) assay was adopted to assess cell viability. The enzyme-linked immunosorbent assay (ELISA) was used to detect the secretion levels of the proinflammatory cytokines IL-6, IL-8, and TNF-α. Transcriptome analysis was conducted to identify the genes that exhibited differential expression between different treatment. The model group included cells treated with LPS (10 µg/mL), the treatment group comprised cells treated with liquiritin (80 µM) and LPS (10 µg/mL), and the control group consisted of untreated cells. To further validate the expression levels of the selected genes, including CSF2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL24, IL6, and LTB, quantitative real-time PCR was performed. The expression of proteins related to the Akt/NF-κB signaling pathway was assessed through western blot analysis. NF-κB nuclear translocation was evaluated through immunofluorescence staining.</p><p><strong>Results: </strong>The secretion of IL-6, IL-8, and TNF-α in LPS-induced HCECs was significantly downregulated by liquiritin. Based on the transcriptome analysis, the mRNA expression of pro-inflammatory cytokines, namely IL-6, IL-8, IL-1β, IL-24, TNF-α, and IL-1α was overproduced by LPS stimulation, and suppressed after liquiritin treatment. Furthermore, the Western blot results revealed a remarkable reduction in the phosphorylation degrees of NF-κB p65, IκB, and Akt upon treatment with liquiritin. Additionally, immunofluorescence analysis confirmed liquiritin's inhibition of LPS-induced p65 nuclear translocation.</p><p><strong>Conclusions: </strong>Collectively, these findings imply that liquiritin suppresses the expression of proinflammatory cytokines, and the anti-inflammatory impacts of liquiritin may be caused by its repression of the Akt/NF-κB signaling pathway in LPS-induced HCECs. These data indicate that liquiritin could provide a potential therapeutic application for inflammation-associated corneal diseases.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"930-941"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Topical Vancomycin is More Efficient than Povidone-Iodine Treatment in Controlling Bacterial Growth in Methicillin-Resistant Staphylococcus Aureus Keratitis Model in Rabbits. 在耐甲氧西林金黄色葡萄球菌兔角膜炎模型中,外用万古霉素比聚维酮碘更有效地控制细菌生长。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-06 DOI: 10.1080/02713683.2024.2349661
Yunus Alkan, Abdulgani Kaymaz, Mustafa Behcet, Abdullah Bayrak
{"title":"Topical Vancomycin is More Efficient than Povidone-Iodine Treatment in Controlling Bacterial Growth in Methicillin-Resistant <i>Staphylococcus Aureus</i> Keratitis Model in Rabbits.","authors":"Yunus Alkan, Abdulgani Kaymaz, Mustafa Behcet, Abdullah Bayrak","doi":"10.1080/02713683.2024.2349661","DOIUrl":"10.1080/02713683.2024.2349661","url":null,"abstract":"<p><p>To evaluate the efficacy of topical vancomycin and povidone iodine (PI) application on methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) keratitis model in rabbits.</p><p><p>MRSA keratitis was induced by injecting 0.1 mL MRSA containing 1000 colony-forming units (CFU) into central cornea of right eyes of 24 New Zealand White rabbits. Animals were divided into four groups (<i>n</i> = 6): control (treated with balanced salt solution), 50 mg/mL topical vancomycin, 5% topical PI, and combination; examined before and after treatment, and corneal tissues were harvested for analysis at 9th hour of treatment.</p><p><p>Bacterial load was determined as: 7.63 ± 0.82 log10 CFU/g in control group, 6.95 ± 1.66 log10 CFU/g in PI group, 4.67 ± 0.77 log10 CFU/g in combination group, and 4.33 ± 0.71 log10 CFU/g in vancomycin group (<i>p</i> = 0.001). Median of total clinical score increased significantly from 7 [range: 5-8] to 11.5 [range: 11-15] (<i>p</i> = 0.001) in control group, did not change (6 [range: 5-8] to 7 [range: 5-7]; <i>p</i> = 0.695) in vancomycin group, increased significantly from 7 [range: 5-8] to 12.5 [range: 10-14] (<i>p</i> < 0.001) in PI group, increased significantly from 6.5 [range: 5-7] to 8 [range: 7-9] in combination group (<i>p</i> = 0.002). Post-treatment clinical scores for chemosis, conjunctival injection, iritis, hypopyon, epithelial erosion, and corneal infiltrate were significantly lower in vancomycin-treated groups compared to others (<i>p</i> < 0.05). In PI-treated groups, especially scores for chemosis, conjunctival injection, epithelial erosion and corneal infiltrate were significantly higher than vancomycin (<i>p</i> < 0.05).</p><p><p>Topical vancomycin significantly inhibited bacterial growth in MRSA keratitis. However, PI was ineffective in controlling this growth; additionally, exerted toxic effect on ocular surface. When vancomycin was combined with PI, no additional increase in efficacy of treatment was detected compared to only vancomycin.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"923-929"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Genetic Variants of Long Noncoding RNA MEG3 and Its Association to the Clinical Features of Diabetic Retinopathy. 长非编码 RNA MEG3 的遗传变异及其与糖尿病视网膜病变临床特征的关系
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-08 DOI: 10.1080/02713683.2024.2350590
Hsiang-Wen Chien, Kai Wang, Shih-Chun Chao, Chia-Yi Lee, Hung-Yu Lin, Shun-Fa Yang
{"title":"The Genetic Variants of Long Noncoding RNA <i>MEG3</i> and Its Association to the Clinical Features of Diabetic Retinopathy.","authors":"Hsiang-Wen Chien, Kai Wang, Shih-Chun Chao, Chia-Yi Lee, Hung-Yu Lin, Shun-Fa Yang","doi":"10.1080/02713683.2024.2350590","DOIUrl":"10.1080/02713683.2024.2350590","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the potential correlation between the single-nucleotide polymorphism (SNP) of <i>maternally expressed gene 3 (MEG3)</i> and the clinical manifestations of diabetic retinopathy (DR).</p><p><strong>Methods: </strong>Five loci of <i>MEG3</i> SNPs including rs4081134 (G/A), rs10144253 (T/C), rs7158663 (G/A), rs3087918 (T/G) and rs11160608 (A/C) were genotyped by TaqMan allelic discrimination in 457 non-DR patients and 280 DR individuals.</p><p><strong>Results: </strong>The distribution frequency of <i>MEG3</i> SNP rs7158663 GA (AOR: 0.683, 95% CI: 0.478-0.975, <i>p</i> = 0.036) and <i>MEG3</i> SNP rs7158663 GA + AA (AOR: 0.686, 95% CI: 0.487-0.968, <i>p</i> = 0.032) were significantly lower in the DR group. And the <i>MEG3</i> SNP rs7158663 GA + AA (AOR: 0.610, 95% CI: 0.377-0.985, <i>p</i> = 0.043) demonstrated a significantly lower distribution frequency in the male DR group. Besides, the DR patients with <i>MEG3</i> SNP rs7158663 GA + AA genotype showed a significantly lower HbA1c level than the DR patients with <i>MEG3</i> SNP rs7158663 GG genotype (7.29 ± 1.23 versus 7.74 ± 1.49, <i>p</i> = 0.013). Moreover, in the analysis using data from gene expression data series database, a higher MEG3 level was significantly correlated to a lower miR-182 level in the database (<i>p</i> = 0.0114).</p><p><strong>Conclusions: </strong>In this study, the distribution frequency of <i>MEG3</i> SNP rs7158663 GA + AA genotype was lower in DR, while the DR would develop under lower HbA1c level in DM patients with this <i>MEG3</i> SNP variant.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"980-987"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-Pseudomonal Effect of Nephrite-Impregnated Contact Lenses. 软玉浸渍隐形眼镜的抗假性效应
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-30 DOI: 10.1080/02713683.2024.2349649
Sangyoon Kim, Eun Jung Kim, Eun Jung Choi, Jin Woo Oh, Jin Hur, Yoon Jeong Kang, Mi Jin Jeong, Hak Sun Yu, Ji Eun Lee
{"title":"Anti-Pseudomonal Effect of Nephrite-Impregnated Contact Lenses.","authors":"Sangyoon Kim, Eun Jung Kim, Eun Jung Choi, Jin Woo Oh, Jin Hur, Yoon Jeong Kang, Mi Jin Jeong, Hak Sun Yu, Ji Eun Lee","doi":"10.1080/02713683.2024.2349649","DOIUrl":"10.1080/02713683.2024.2349649","url":null,"abstract":"<p><strong>Purpose: </strong>This study compared the anti-pseudomonal effects between nephrite-impregnated contact lenses (CLs) and conventional and cosmetic CLs.</p><p><strong>Methods: </strong>After inoculation with <i>Pseudomonas aeruginosa (P.aeruginosa)</i>, we counted the number of bacteria on the CL surface and observed each surface using atomic force microscopy (AFM) and scanning electron microscopy (SEM). To estimate potential harm of nephrite-impregnated CLs, we conducted a safety test using a rabbit model, treated with all CL types.</p><p><strong>Results: </strong>Both conventional and cosmetic CLs (<i>n</i> = 258 ± 2.9 × 10<sup>4</sup>, 368 ± 2.2 × 10<sup>4</sup>) showed significantly decreased number of attached bacteria when compared with those without nephrite impregnation (<i>n</i> = 134 ± 0.8 × 10<sup>4</sup>, 238 ± 2.5 × 10<sup>4</sup>, <i>p</i> < 0.0001, respectively). AFM and SEM revealed that <i>P. aeruginosa</i> was less attached to the nephrite-impregnated CLs than to the conventional and cosmetic CLs, although those with nephrite impregnation had rougher surface. In the safety test, there were no significant differences in the findings between four groups, and the clarity and stability of all corneas were preserved.</p><p><strong>Conclusions: </strong>Nephrite may be used as a next-generation substance to reduce infectious keratitis caused by <i>P. aeruginosa</i> when added to CLs.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"914-922"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optical Coherence Tomography Angiography in Vogt-Koyanagi-Harada Patients: A Systematic Review and Meta-Analysis. Vogt-Koyanagi-Harada 患者的光学相干断层扫描血管造影:系统综述与元分析》。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-03-08 DOI: 10.1080/02713683.2024.2327054
Ruyu Liang, Jiawei Geng, Hai Jiang, Siyan Jin, Xia Wang, Xiaoli Liu
{"title":"Optical Coherence Tomography Angiography in Vogt-Koyanagi-Harada Patients: A Systematic Review and Meta-Analysis.","authors":"Ruyu Liang, Jiawei Geng, Hai Jiang, Siyan Jin, Xia Wang, Xiaoli Liu","doi":"10.1080/02713683.2024.2327054","DOIUrl":"10.1080/02713683.2024.2327054","url":null,"abstract":"<p><strong>Purpose: </strong>To describe the choroidal retinal microvascular system in Vogt-Koyanagi-Harada (VKH) subjects and furnish additional proof for the early authentication and treatment of VKH suffers.</p><p><strong>Methods: </strong>From the beginning to July 2023, a comprehensive search for issued articles on optical coherence tomography angiography (OCTA) among VKH sufferers was implemented in Embase, PubMed, and Web of Science databases. This meta-analysis included 9 eligible studies. Primary endpoints included four kinds of vascular densities, such as superficial capillaris plexus (SCP), deep capillaris plexus (DCP), and choriocapillary (CC). In addition to these, there were foveal avascular zone (FAZ), central retinal thickness (CT), best-corrected distance visual acuity (BCVA log MAR), and subfoveal choroidal thickness (SFCT).</p><p><strong>Results: </strong>SCP and DCP vessel densities in maculas were both smaller in VKH sufferers in the active stage than those normal and remission examinees (SCP vessel density, <i>p</i> < 0.00001, DCP vessel density, <i>p</i> < 0.00001). Compared to remission, CC vascular density was lower during the active phase. (<i>p</i> < 0.00001). SFCT and CT in the active phase exceeded those in normal and remission examinees (all of them <i>p</i> < 0.00001). In terms of the patients with remission, their FAZ was bigger than that in normal subjects. (MD =0.04, <i>p</i> < 0.0001).</p><p><strong>Conclusions: </strong>Retinal and choroidal microvasculatures are characteristically changed in active VKH patients, which suggests that OCTA can be used as a tool for VKH follow-up.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"895-904"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140058892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Refractive Astigmatism Outcomes of Femtosecond Laser-Assisted Arcuate Keratotomies Combined with Femtosecond Laser-Assisted Cataract Surgery: Two-Year Results. 飞秒激光辅助弧形角膜切开术联合飞秒激光辅助白内障手术的屈光散光效果:两年结果。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-23 DOI: 10.1080/02713683.2024.2353268
Umar Sandhu, Andrew R Osborn, Deanna H Dang, David A Murphy, Achim Langenbucher, Jascha A Wendelstein, Kamran M Riaz
{"title":"Refractive Astigmatism Outcomes of Femtosecond Laser-Assisted Arcuate Keratotomies Combined with Femtosecond Laser-Assisted Cataract Surgery: Two-Year Results.","authors":"Umar Sandhu, Andrew R Osborn, Deanna H Dang, David A Murphy, Achim Langenbucher, Jascha A Wendelstein, Kamran M Riaz","doi":"10.1080/02713683.2024.2353268","DOIUrl":"10.1080/02713683.2024.2353268","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effectiveness and stability of refractive astigmatism reduction after penetrating femtosecond laser-assisted arcuate keratotomy performed at the time of femtosecond laser-assisted cataract surgery.</p><p><strong>Methods: </strong>Non-randomized retrospective data analysis of all patients that underwent femtosecond laser-assisted cataract surgery with femtosecond laser-assisted arcuate keratotomy over a 4-year period with a non-toric monofocal intraocular lens (2017-2021) at a tertiary care academic center. Postoperative visual acuity, manifest refraction, and predicted residual refractive error were also recorded at 1 month, 3-6 months, 12-18 months, and 2 years postoperatively. Preoperative keratometric astigmatism was compared to postoperative refractive astigmatism using vector calculations and the ASCRS double-angle plot tool.</p><p><strong>Results: </strong>This study comprised 266 eyes (179 patients) that met inclusion criteria. The mean preoperative keratometric astigmatism magnitude was 0.99 ± 0.53 D. At 1 month, 3-6 months, 12-18 months, and 2 years postoperatively, the mean refractive cylinder was 0.49 ± 0.45 D, 0.49 ± 0.45 D, 0.55 ± 0.54 D, and 0.52 ± 0.46 D, respectively. Horizontal against-the-rule astigmatism showed a higher tendency toward undercorrection than vertical with-the-rule astigmatism, which had a slightly higher tendency toward overcorrection. With-the-rule astigmatism had smaller difference vectors between target-induced astigmatism and surgically induced astigmatism.</p><p><strong>Conclusions: </strong>Femtosecond laser-assisted arcuate keratotomy performed at the time of femtosecond laser-assisted cataract surgery was an effective option for correcting low-to-moderate corneal astigmatism for up to 2 years.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"961-971"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sestrin2 Protects Human Lens Epithelial Cells (HLECs) Against Apoptosis in Cataracts Formation: Interaction Between Endoplasmic Reticulum (ER) Stress and Oxidative Stress (OS) is Involved. Sestrin2 保护人类晶状体上皮细胞(HLECs)免受白内障形成过程中的细胞凋亡:内质网(ER)应激和氧化应激(OS)之间的相互作用参与其中。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-09-01 Epub Date: 2024-05-23 DOI: 10.1080/02713683.2024.2352058
Di Sun, Hui Cui, Liyuan Rong, Tianju Ma, Xuanlong Li, Zi Ye, Zhaohui Li
{"title":"Sestrin2 Protects Human Lens Epithelial Cells (HLECs) Against Apoptosis in Cataracts Formation: Interaction Between Endoplasmic Reticulum (ER) Stress and Oxidative Stress (OS) is Involved.","authors":"Di Sun, Hui Cui, Liyuan Rong, Tianju Ma, Xuanlong Li, Zi Ye, Zhaohui Li","doi":"10.1080/02713683.2024.2352058","DOIUrl":"10.1080/02713683.2024.2352058","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the correlation of endoplasmic reticulum stress (ERS) and oxidative stress (OS), and the protective effect of Sestrin2 (SESN2) on human lens epithelial cells (HLECs).</p><p><strong>Methods: </strong>Tunicamycin (TM) was used to induce ERS in HLECs. 4-Phenylbutyric acid (4-PBA) was used to inhibit ERS. Eupatilin applied to HLECs as SESN2 agonist. SESN2 expression was knocked down <i>via</i> si-RNA in HLECs. The morphological changes of HLECs were observed by microscope. ER-tracker to evaluate ERS, ROS production assay to measure ROS, flow cytometry to calculate cell apoptosis rate. Immunofluorescence to observe Nrf2 translocation, and effects of TM or EUP on SESN2. Western blot and qPCR were used to evaluate the expression of GRP78, PERK, ATF4, CHOP, Nrf2, and SESN2 expression in HLECs with different treatment groups.</p><p><strong>Results: </strong>ERS can elevate the expression of ROS and Nrf2 to induce OS. Upregulation of SESN2 was observed in ERS-mediate OS. Overexpression of SESN2 can reduce the overexpression of ERS-related protein GRP78, PERK, ATF4, proapoptotic protein CHOP, OS-related protein Nrf2, as well as ROS, and alleviate ERS injury at the same time. Whereas knockdown of SESN2 can upregulate the expression of GRP78, PERK, ATF4, CHOP, Nrf2, ROS, and deteriorate ERS damage.</p><p><strong>Conclusions: </strong>ERS can induce OS, they form a vicious cycle to induce apoptosis in HLECs, which may contribute to cataract formation. SESN2 could protect HLECs against the apoptosis by regulating the vicious cycle between ERS and OS.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"949-960"},"PeriodicalIF":1.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macular Optical Coherence Tomography Angiographic Study in Children with a History of Prematurity: A Systematic Review and Meta-Analysis. 有早产史儿童的黄斑光学相干断层扫描血管造影研究:系统回顾与元分析》。
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-08-29 DOI: 10.1080/02713683.2024.2397034
Samira Chaibakhsh, Ali Aghajani, Maryam Moradi, Kaveh Abri Aghdam
{"title":"Macular Optical Coherence Tomography Angiographic Study in Children with a History of Prematurity: A Systematic Review and Meta-Analysis.","authors":"Samira Chaibakhsh, Ali Aghajani, Maryam Moradi, Kaveh Abri Aghdam","doi":"10.1080/02713683.2024.2397034","DOIUrl":"https://doi.org/10.1080/02713683.2024.2397034","url":null,"abstract":"<p><strong>Purpose: </strong>to assess the changes in the macular structure in children with history of prematurity and to find the relationship between retinopathy of prematurity (ROP) treatment and macular ultrastructural changes found in optical coherence tomography angiography study.</p><p><strong>Methods: </strong>a search for identifying relevant studies published in English using PubMed, Google Scholar, Scopus, and Web of Science databases from the date of inception to 21 October 2022 was conducted. Studies were included if their subjects were >5 years and used Optovue device for imaging. Twelve studies were included for final analysis. After extracting data, foveal avascular zone (FAZ), vessel density (VD) of superficial capillary plexus (SCP) and deep capillary plexus (DCP) in the foveal and parafoveal area, central foveal thickness (CFT), and visual acuity (VA) were compared between the study groups.</p><p><strong>Results: </strong>in individual who were born full term the FAZ was significantly larger. The SCP and DCP VD was significantly higher in children with history of ROP treatment. Superficial parafoveal VD was significantly lower in term children than both treated groups. The CFT was significantly higher in children with history of ROP (treated and untreated) than the terms. VA was lower in laser and IVI treated children than terms and it was related to the changes in CFT, foveal superficial and deep VD and FAZ area.</p><p><strong>Conclusions: </strong>Patients with a history of ROP treatment have a significantly greater CFT, higher VD of foveal SCP and DCP, and lower VA than the term-born children. Furthermore, the FAZ is negatively associated with VA and CFT.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analyzing Tear Fluid Composition by Synchronous Fluorescence for Diagnosing Dry Eye Disease and the Role of Phytotherapy Intervention. 利用同步荧光分析泪液成分以诊断干眼症及植物疗法干预的作用
IF 1.7 4区 医学
Current Eye Research Pub Date : 2024-08-01 Epub Date: 2024-04-28 DOI: 10.1080/02713683.2024.2344184
Shaimaa M Moussa, Sherif S Mahmoud, Eman M Aly, Mona S Talaat
{"title":"Analyzing Tear Fluid Composition by Synchronous Fluorescence for Diagnosing Dry Eye Disease and the Role of Phytotherapy Intervention.","authors":"Shaimaa M Moussa, Sherif S Mahmoud, Eman M Aly, Mona S Talaat","doi":"10.1080/02713683.2024.2344184","DOIUrl":"10.1080/02713683.2024.2344184","url":null,"abstract":"<p><strong>Purpose: </strong>Tear fluid gained attention as a representative biological fluid. Its simple and non-invasive collection methods as well as richness of candidate biomarkers made it a potential diagnostic tool for different diseases such as dry eye. Synchronous fluorescence spectroscopy is a highly sensitive analytical tool that results in narrowing and enhanced peak resolution, and has a potential role in disease diagnosis, biomarker identification, and therapeutic monitoring. We applied synchronous fluorescence spectroscopy to monitor variations of tear fluid composition during the development of dry eye disease and to evaluate the potential effects of phytotherapy.</p><p><strong>Methods: </strong>Dry eye model was induced in Chinchilla rabbits by instillation of 1% atropine sulfate ophthalmic solution. Then, the tear fluid was collected at 3, 7, and 14 days and subjected to synchronous fluorescence spectroscopy. Phytotherapy was achieved by topical instillation of 20 µl of water extracts of pomegranate peel or green tea powders.</p><p><strong>Results: </strong>The fluorescence results revealed changes in the structure of tear fluid over time and the eye is subjected to toxification due to oxidative stress. In addition, dry eye disease was found to affect the metabolic/energetic state of the eye. On the other hand, phytotherapy led to enhancement of the metabolic/biosynthesis state due to activation of flavin adenine dinucleotide-associated proteins.</p><p><strong>Conclusion: </strong>There was change in the electrical conductivity of tear fluid proteins. In the case of dry eyes, they became electrical insulators, while in the case of treatment with extracts, their electrical conductivity properties improved. The effects of phytotherapy can be related to the high content of ellagic acid and anthocyanin of pomegranate extract, while in green tea, they are related to catechins and phenolic compounds.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"826-834"},"PeriodicalIF":1.7,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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