Current Eye Research最新文献

{"title":"Adjusting Laser Parameters for Myopic Laser Blended Vision According to Laser Treatment Zone: A Retrospective Study.","authors":"Anke Messerschmidt-Roth, Walter Sekundo, Karsten Pfeiffer, Alexandra Stein, Felix Mathias Wagner","doi":"10.1080/02713683.2025.2473565","DOIUrl":"10.1080/02713683.2025.2473565","url":null,"abstract":"<p><strong>Purpose: </strong>The nomograms for the laser procedure \"Laser Blended Vision\" (Presbyond^®) for myopia were developed based on a 6 mm zone. The aim of these studies is to demonstrate that customized nomograms are necessary when changing the optical zone. The three-month results after the Presbyond^® correction using a 6.4 mm to 6.5 mm optical zone were analyzed in myopic patients. All patients were treated at the University Hospital Gießen and Marburg and for analyses purposes they were divided into two groups. Group 1 was treated from 2017-2019 without a nomogram for this larger optical zone, and group 2 from 2019-2022 with a customized nomogram.</p><p><strong>Methods: </strong>In the retrospective monocentric study, 86 eyes from 43 patients in group 1 and 120 eyes from 60 patients in group 2 were analyzed. All eyes were treated with the 500 kHz VisuMax^® Laser and the Mel90^® Excimer Laser using a 6.4 mm to 6.5 mm optical zone. The results are collected using Datagraph^® software.</p><p><strong>Results: </strong>The three-month results show overcorrection in both groups concerning the targeted refractive goal. In group 2, the analysis of distance and near vision shows that eyes treated for near vision deviate more than predicted. This is also confirmed in the refractive outcome. However, patient satisfaction is comparable in both groups, and retreatment rate is lower than published, although higher in group 1 than in group 2.</p><p><strong>Conclusion: </strong>Since PRESBYOND^® targets both, good distance vision does not fully reflect the goal of this procedure. The importance of personalized nomograms for distance and near vision is supported by our results, also the retreatment rate improved, and patient satisfaction influences the decision for retreatment. A separate nomogram for the distance and reading eye to further improve correction predictability is necessary, as predictability in both eyes is differing.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"710-718"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of circFAM53B in Regulating Choroidal Vascular Function in Pathological Myopia.","authors":"Meng Guan, Boyong Zhang, Wenjing Wu, Yu Li, Fengju Zhang","doi":"10.1080/02713683.2025.2477550","DOIUrl":"10.1080/02713683.2025.2477550","url":null,"abstract":"<p><strong>Purpose: </strong>Myopia has reached epidemic levels worldwide, in which pathological myopia can lead to irreversible visual loss from associated ocular complications. This study aimed to investigate the role of circular RNA circFAM53B in choroidal dysfunction in pathological myopia progression.</p><p><strong>Methods: </strong>We established <i>In vitro</i> and <i>in vivo</i> models to simulate hypoxic and oxidative injuries to rhesus macaque choroid-retina endothelial cells, which may contribute to the choroidal vascular dysfunction in pathological myopia.</p><p><strong>Results: </strong>RNA sequencing and comprehensive bioinformatics analyses revealed widespread differential expression of circular RNAs in injured choroidal cells, with circFAM53B being notably and consistently upregulated under both hypoxic and oxidative conditions. Functional assays demonstrated that small interfering RNA (siRNA)-mediated knockdown of circFAM53B significantly enhanced viability, migration and tubulogenesis of choroidal endothelial cells while suppressing apoptosis. Mechanistic studies found that circFAM53B can act as a sponge for miR-1248, consequently relieving the inhibition of miR-1248 on its target <i>THBS1</i> and leading to <i>THBS1</i> upregulation. Form-deprivation myopia in guinea pigs also showed substantially elevated circFAM53B expression in myopic eye tissues over time.</p><p><strong>Conclusions: </strong>Our results shed light on the involvement of the circFAM53B/miR-1248/THBS1 pathway in the decline of choroidal function observed in pathological myopia, expanding current understanding of the molecular mechanisms driving myopia development and offering potential therapeutic targets for choroid-related myopia.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"758-770"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tear Global Metabolomic Differences in Pre-Operative Cataract Patients With and Without Dry Eye Disease.","authors":"Morten Gundersen, Christian Nilsen, Per Jensen, Mazyar Yazdani, Øygunn Utheim, Elise Sandås Sand, Helge Rootwelt, Kjell Gunnar Gundersen, Katja Benedikte Prestø Elgstøen","doi":"10.1080/02713683.2025.2472369","DOIUrl":"10.1080/02713683.2025.2472369","url":null,"abstract":"<p><strong>Purpose: </strong>The primary aim of this article was to investigate differences in the metabolomic profile of tear fluid obtained from pre-operative cataract patients, with or without dry eye disease. The objective was to look for metabolomic signatures that might discriminate between the two groups.</p><p><strong>Methods: </strong>A total of 222 patients were enrolled in the study. Eighty-one were randomly selected for metabolomic analysis from both dry eye positive and dry eye negative groups, categorized prior to cataract surgery. Tear film was collected using Schirmer-1 strips and analyzed using an optimized method developed for low-volume Schirmer samples and allowing for repeated analyses, including other -omics approaches at a later stage. Metabolomic data were collected using a global liquid chromatography-mass spectrometry method. Samples were compared using principal component analysis and volcano plots to look for overall global differences as well as group-specific metabolic signatures.</p><p><strong>Results: </strong>All samples were analyzed with a high number of features identified. No group-specific clustering was observed in principal component analysis for the dry eye positive or dry eye negative groups. However, volcano plots revealed that a majority of the metabolomic features had lower concentration in the dry eye positive group compared to the dry eye negative group. Four of these features had a Log₂-fold change ≤ -1 and <i>p</i> value ≤.05. These warrant further study.</p><p><strong>Conclusion: </strong>Although no overall global difference was observed on the principal component analysis plots, a general trend of lower metabolite concentrations in the dry eye disease group was shown. Moreover, several metabolites of interest were discovered with significantly different signal intensities between the groups. These metabolites may aid future diagnostics and serve as possible biomarkers and therapeutic targets for dry eye disease in pre-operative cataract patients.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"579-589"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cyclosporine Eye Drop Use on the Success of Punctoplasty in Acquired Punctal Stenosis.","authors":"Melek Mutlu Sönmez, Elif Ertan Baydemir","doi":"10.1080/02713683.2025.2466659","DOIUrl":"10.1080/02713683.2025.2466659","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of cyclosporine-A (CsA) 0.05% eye drops for management of acquired punctal stenosis, either alone or in combination with punctoplasty.</p><p><strong>Methods: </strong>In this prospective study patients referred to the oculoplastics clinic with acquired punctal stenosis (APS) were divided into three groups. Group A consisted of patients treated with CsA, Group B consisted of patients who underwent punctoplasty, and Group C included patients treated with both CsA and punctoplasty. Munk score, fluorescein dye disappearence test (FDDT), punctal opening grading were evaluated at baseline, and at the 1st and 6th month follow-ups.</p><p><strong>Results: </strong>One hundred-eleven eyes of 80 patients were included in the study. While the punctum grade was significantly higher, the FDDT grade and Munk scores were significantly lower in group C compared to groups A and B at the 1st and 6th month follow-ups. The functional success rate was 69%, 79%, and 88% (<i>p</i> = .01), and the anatomical success rate was 77%, 77%, and 90% (<i>p</i> = .03) in Group A, B, and C, respectively, at the 6th month follow-up.</p><p><strong>Conclusions: </strong>In the treatment of APS, the use of CsA alone and the punctoplasty procedure demonstrated comparable anatomical and functional success rates at the 6th month follow-up. However, combining CsA with punctoplasty enhances both anatomical and functional success rates.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"651-655"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function of AMPK/mTOR Signaling in TGF-β1-Induced Pterygium Fibroblast Proliferation and Transdifferentiation.","authors":"Yun Hua, Xinrong Zhao, Na Tang, Huijuan Wan, Haidong Lian, Ting Yuan, Chao Si","doi":"10.1080/02713683.2025.2470410","DOIUrl":"10.1080/02713683.2025.2470410","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the regulatory role of the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR) signaling pathway in mediating transforming growth factor-beta 1 (TGF-β1)-induced cellular proliferation and transdifferentiation processes in human pterygium fibroblasts (HPFs).</p><p><strong>Methods: </strong>HPFs were stimulated with TGF-β1 <i>in vitro</i>. Cell viability was assessed using the CCK-8 assay at 12/24/48-h post-stimulation, while migratory capacity was evaluated through standardized wound healing assays. Quantitative real-time PCR (qPCR) and western blotting analyses were employed to evaluate the expression of proliferation marker proliferating cell nuclear antigen (PCNA) and myofibroblast transdifferentiation biomarker α-smooth muscle actin (α-SMA). Western blotting further characterized the activation status of AMPK/mTOR signaling by quantifying phosphorylated AMPK (p-AMPK) and phosphorylated mTOR (p-mTOR), with total AMPK and mTOR levels serving as loading controls. To establish mechanistic causality, TGF-β1-primed HPFs were modulated using the AMPK inhibitor Compound C and activator AICAR for 24 h. Functional consequences were analyzed through CCK-8 viability assays and wound healing assays, while molecular correlates were assessed <i>via</i> qPCR and western blotting for PCNA, α-SMA, and pathway components. This comprehensive approach delineated the AMPK/mTOR axis as a critical regulator of TGF-β1-driven fibrotic phenotype acquisition in HPFs.</p><p><strong>Results: </strong>Following TGF-β1 pretreatment-induced activation of human HPFs, both cell viability and migratory capacity were markedly enhanced, with concomitant upregulation of PCNA and α-SMA. Compound C-mediated AMPK inhibition potentiated the TGF-β1-induced enhancements in HPFs viability and migration rate, concomitant with reduced p-AMPK/AMPK ratio and elevated expression of PCNA, α-SMA, and p-mTOR/mTOR ratio. Conversely, AICAR-driven AMPK activation attenuated TGF-β1-stimulated effects, demonstrating diminished viability, suppressed migratory capacity, increased p-AMPK/AMPK ratio, and decreased expression of PCNA, α-SMA, and p-mTOR/mTOR ratio.</p><p><strong>Conclusions: </strong>This study demonstrates the critical regulatory role of the AMPK/mTOR signaling pathway in controlling TGF-β1-induced proliferation and transdifferentiation in HPFs, thereby providing a potential mechanistic framework for developing novel therapeutic interventions targeting fibrotic ocular surface disorders.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"600-609"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Pupillary Responses in Healthy Eyes and Eyes Implanted with Multifocal or Monofocal Intraocular Lenses.","authors":"Kohei Miyata, Shinichiro Kobayakawa","doi":"10.1080/02713683.2025.2469833","DOIUrl":"10.1080/02713683.2025.2469833","url":null,"abstract":"<p><strong>Purpose: </strong>Comparison of dynamic pupillary parameters (DPPs) between healthy volunteers and multi- or monofocal intraocular lens (IOL) implanted eyes, and examination of the correlation between postoperative visual acuity (VA), contrast sensitivity (CS), visual dysphotopsias (VD), and these parameters in IOL-implanted eyes.</p><p><strong>Methods: </strong>This cross-sectional study classified and analyzed DPPs from healthy volunteers (308 eyes) according to age. In the multifocal IOL group (119 eyes), 51 eyes were implanted with Lentis Comfort^® (L group), 32 with PanOptix^® (P group), and 36 with Synergy^® (S group). The monofocal IOL group included 33 eyes implanted with Vivinex Impress^® (V group). DPPs by the pupillometer (PLR-3000), distance, intermediate and near VA, and CS and VD were examined one month postoperatively in 152 eyes.</p><p><strong>Results: </strong>Significant decreases in maximum or minimum pupillary diameter (INIT, END), percentage of pupillary constriction (DELTA), latency between light stimulation and constriction (LAT), and average and maximum constriction velocity (ACV, MCV), average dilation velocity (ADV), were observed with aging in the healthy eyes group. The preoperative group showed a significant age-related decrease in DELTA and increase in LAT. The postoperative group showed a significant age-related decrease in INIT, END, and ADV. The L group exhibited a significant positive correlation between ADV and uncorrected intermediate VA (UIVA), while the P group exhibited a significant positive correlation between ACV and UIVA, and the S group exhibited a significant negative correlation between ACV and uncorrected near VA. For CS, the S group exhibited a significant positive correlation with ADV at 1.0 and 0.7 degrees, and the P group exhibited a significant negative correlation between ACV and glare.</p><p><strong>Conclusion: </strong>DPPs are greatly affected by age. DPPs for multi- or monofocal IOL eyes correlate with uncorrected VA, CS, and VD.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"610-617"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choriocapillaris Density Remains Constant in Healthy Older Adults After 24 Weeks of High-Speed Circuit Training.","authors":"Kevin K Zhou, Ava-Gaye Simms, Alessandro Gregori, Rosalia Parrino, Abdulrahman Mamoon Allaf, Joseph F Signorile, Jianhua Wang, Hong Jiang","doi":"10.1080/02713683.2025.2469226","DOIUrl":"10.1080/02713683.2025.2469226","url":null,"abstract":"<p><strong>Purpose: </strong>The goal of this study was to assess the changes in choriocapillaris perfusion density (CCD) after 24 weeks of structured high-speed circuit resistance training (HSCT) in healthy older adults.</p><p><strong>Methods: </strong>A total of 30 healthy, cognitively normal adults, 60 years old and above, were recruited for the study. The participants were randomized to either the HSCT group (<i>n</i> = 15) or the control group (CON, <i>n</i> = 15). The HSCT group trained 3 times a week on non-consecutive days for 24 weeks, while the CON group did not perform formal training. Optical coherence tomography angiography (OCTA) was used to image both eyes of each study participant at baseline and 24-week follow-up. The follow-up session was completed within 3 days of the last exercise session for the HSCT group. The CCD was measured within a 2.5-mm in diameter centered on the fovea.</p><p><strong>Results: </strong>There were no significant differences in CCD between baseline and 24-week follow-up in either the HSCT group or the CON group. In the HSCT group, the CCD was 62.6 ± 6.1% (mean ± SD) at baseline and 63.7 ± 6.1% at the 24-week follow-up (<i>p</i> = 0.21). In the CON group, the CCD was 62.8 ± 5.6% at baseline and 63.8 ± 4.9% at 24 weeks (<i>p</i> = 0.18).</p><p><strong>Conclusion: </strong>CCD was unchanged after a 24-week high-speed circuit resistance training in healthy older individuals, suggesting possible regulatory maintenance of choroidal blood flow. Further research with multiple age cohorts may be necessary to corroborate these findings.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"624-630"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Conjunctival Cell HLA-DR Expression with the Severity of Signs and Symptoms of Dry Eye Disease at Baseline in the NORTHERN LIGHTS Phase 2 Trial.","authors":"Eric J Kuklinski, Neeta S Roy, Morten Præstegaard, Yi Wei, Penny A Asbell","doi":"10.1080/02713683.2025.2469254","DOIUrl":"10.1080/02713683.2025.2469254","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the association of HLA-DR on ocular surface conjunctival cells with the severity of signs and symptoms of dry eye disease (DED) in patients enrolled in the NORTHERN LIGHTS phase 2 trial.</p><p><strong>Methods: </strong>Patients with moderate to severe DED were recruited from 10 different countries in Europe. Symptoms of DED were assessed using the visual analog scale (VAS) global discomfort score, and signs <i>via</i> corneal fluorescein staining (CFS), lissamine green staining, Schirmer's test, and tear osmolarity. HLA-DR expression was evaluated <i>via</i> flow cytometry from impression cytology of conjunctival cells using the EyePrim™ device sampled from 205 DED patients.</p><p><strong>Results: </strong>HLA-DR expression was detected in a mean of 3.4% of cells at baseline. HLA-DR expression was significantly associated with corneal staining severity at baseline (<i>p</i> < 0.01). HLA-DR expression was not significantly associated with DED symptoms (VAS global discomfort score), nor lissamine green staining, Schirmer's test, tear osmolarity, or Sjogren's syndrome.</p><p><strong>Conclusion: </strong>HLA-DR was present in a wide range of percentages among DED patients indicating the heterogeneous nature of DED. HLA-DR was significantly associated with corneal fluorescein staining suggesting a correlation of the biomarker with ocular surface damage.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"567-571"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Keratoconus Progression Rate between Adolescents Aged 19-24 Years and Young Adults: Impact on Indication for Crosslinking.","authors":"Pinar Kosekahya, Elias Flockerzi, Cristian Munteanu, Haris Sideroudi, Berthold Seitz","doi":"10.1080/02713683.2025.2470408","DOIUrl":"10.1080/02713683.2025.2470408","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the keratoconus characteristics and progression rate in a patient group of adolescents aged 19-24 years and to compare the results with young adults aged 25-30 years.</p><p><strong>Methods: </strong>A total of 158 keratoconic eyes (82 and 76 eyes in the adolescent and young adult groups) of the Homburg Keratoconus Center, which were examined by Scheimpflug tomography at least two times, were included in this retrospective study. The visual characteristics, corneal tomography, and biomechanical measurements were noted at the initial visit, 6 months, 12 months, and 24 months after the initial visit. Progression rates and the amounts of change were calculated and compared between groups.</p><p><strong>Results: </strong>Baseline visual, tomographical, and biomechanical characteristics along with keratoconus staging were similar between groups at the initial visit (<i>p</i> > .05 for all values). Progression rate was 76.8% vs 14.9% with respect to Kmax change, 72.3% vs 12.0% with respect to ABC parameters, and 26.4% vs 16.4% with respect to E staging in the adolescent vs young adult groups, respectively (<i>p</i> < .001, <i>p</i> < .001, and <i>p</i> = .25).</p><p><strong>Conclusions: </strong>Keratoconus may worsen more rapidly in adolescent patients aged 19-24 years compared to young adult patients aged 25-30 years. This study emphasizes the high progression rate in adolescent patients and the consideration of early cross-linking and/or at least closer follow-ups in this age group.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"572-578"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Biomarkers for Screening Retinal Detachment Associated with Choroidal Detachment Using DIA-MS-Based Proteomics.","authors":"Pingping Li, Mengyao Han, Rui Zhang, Fangyu Chen, Yanzi Li, Jing Yuan, Ning Ma, Lu Li, Jianhua Wu","doi":"10.1080/02713683.2025.2469228","DOIUrl":"10.1080/02713683.2025.2469228","url":null,"abstract":"<p><strong>Purpose: </strong>Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is known for its rapid progression and poor prognosis, making it a subject of significant clinical interest due to its complex pathogenesis. This study aims to utilize mass spectrometry for proteomic analysis of vitreous humor to identify proteins and biomarkers critical to the pathophysiology of RRDCD.</p><p><strong>Methods: </strong>Data-independent acquisition (DIA) mass spectrometry was employed to analyze vitreous humor samples from RRDCD and Rhegmatogenous retinal detachment (RRD) patients. The analysis focused on identifying differentially expressed proteins (DEPs) and determining their functional roles. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore the biological processes and pathways associated with these DEPs. DEPs were validated using ELISA to confirm the reliability of the mass spectrometry results.</p><p><strong>Results: </strong>A total of 237 DEPs were identified, including 63 upregulated and 174 downregulated proteins. GO functional analysis showed enrichment in terms related to molecular function regulators, biological adhesion, and the extracellular region. KEGG pathway analysis revealed significant associations with the Extracellular environment (ECM)-receptor interaction, complement and coagulation cascades, and lysosome pathways. Receiver operating characteristic (ROC) analysis further confirmed that Serum amyloid A-4 protein (SAA4), Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1), and Vitronectin (VTN) exhibit excellent performance in the diagnosis of RRDCD. Both VTN and SAA4 showed positive correlations with BCVA at 6 months post-surgery.</p><p><strong>Conclusion: </strong>RRDCD activates a variety of cellular pathways, not only complement and inflammation, but also the remodeling of the extracellular matrix and the activation of lysosome-related pathways disrupt normal retinal cell function. SAA4, ITIH1, and VTN in vitreous fluid can serve as effective biomarkers for diagnosing patients with RRDCD. Additionally, both VTN and SAA4 are correlated with post-operative visual outcomes.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"641-650"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}