Current Eye Research最新文献

{"title":"Comparison of Keratoconus Progression Rate between Adolescents Aged 19-24 Years and Young Adults: Impact on Indication for Crosslinking.","authors":"Pinar Kosekahya, Elias Flockerzi, Cristian Munteanu, Haris Sideroudi, Berthold Seitz","doi":"10.1080/02713683.2025.2470408","DOIUrl":"10.1080/02713683.2025.2470408","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the keratoconus characteristics and progression rate in a patient group of adolescents aged 19-24 years and to compare the results with young adults aged 25-30 years.</p><p><strong>Methods: </strong>A total of 158 keratoconic eyes (82 and 76 eyes in the adolescent and young adult groups) of the Homburg Keratoconus Center, which were examined by Scheimpflug tomography at least two times, were included in this retrospective study. The visual characteristics, corneal tomography, and biomechanical measurements were noted at the initial visit, 6 months, 12 months, and 24 months after the initial visit. Progression rates and the amounts of change were calculated and compared between groups.</p><p><strong>Results: </strong>Baseline visual, tomographical, and biomechanical characteristics along with keratoconus staging were similar between groups at the initial visit (<i>p</i> > .05 for all values). Progression rate was 76.8% vs 14.9% with respect to Kmax change, 72.3% vs 12.0% with respect to ABC parameters, and 26.4% vs 16.4% with respect to E staging in the adolescent vs young adult groups, respectively (<i>p</i> < .001, <i>p</i> < .001, and <i>p</i> = .25).</p><p><strong>Conclusions: </strong>Keratoconus may worsen more rapidly in adolescent patients aged 19-24 years compared to young adult patients aged 25-30 years. This study emphasizes the high progression rate in adolescent patients and the consideration of early cross-linking and/or at least closer follow-ups in this age group.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"572-578"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Biomarkers for Screening Retinal Detachment Associated with Choroidal Detachment Using DIA-MS-Based Proteomics.","authors":"Pingping Li, Mengyao Han, Rui Zhang, Fangyu Chen, Yanzi Li, Jing Yuan, Ning Ma, Lu Li, Jianhua Wu","doi":"10.1080/02713683.2025.2469228","DOIUrl":"10.1080/02713683.2025.2469228","url":null,"abstract":"<p><strong>Purpose: </strong>Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is known for its rapid progression and poor prognosis, making it a subject of significant clinical interest due to its complex pathogenesis. This study aims to utilize mass spectrometry for proteomic analysis of vitreous humor to identify proteins and biomarkers critical to the pathophysiology of RRDCD.</p><p><strong>Methods: </strong>Data-independent acquisition (DIA) mass spectrometry was employed to analyze vitreous humor samples from RRDCD and Rhegmatogenous retinal detachment (RRD) patients. The analysis focused on identifying differentially expressed proteins (DEPs) and determining their functional roles. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore the biological processes and pathways associated with these DEPs. DEPs were validated using ELISA to confirm the reliability of the mass spectrometry results.</p><p><strong>Results: </strong>A total of 237 DEPs were identified, including 63 upregulated and 174 downregulated proteins. GO functional analysis showed enrichment in terms related to molecular function regulators, biological adhesion, and the extracellular region. KEGG pathway analysis revealed significant associations with the Extracellular environment (ECM)-receptor interaction, complement and coagulation cascades, and lysosome pathways. Receiver operating characteristic (ROC) analysis further confirmed that Serum amyloid A-4 protein (SAA4), Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1), and Vitronectin (VTN) exhibit excellent performance in the diagnosis of RRDCD. Both VTN and SAA4 showed positive correlations with BCVA at 6 months post-surgery.</p><p><strong>Conclusion: </strong>RRDCD activates a variety of cellular pathways, not only complement and inflammation, but also the remodeling of the extracellular matrix and the activation of lysosome-related pathways disrupt normal retinal cell function. SAA4, ITIH1, and VTN in vitreous fluid can serve as effective biomarkers for diagnosing patients with RRDCD. Additionally, both VTN and SAA4 are correlated with post-operative visual outcomes.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"641-650"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Famciclovir Eye Drop Formulations for Herpes Zoster Infections.","authors":"Iris M Domenech-Monsell, Adrián M Alambiaga-Caravaca, Lucía Bernat-Just, Virginia Merino, Vicent Rodilla, Cristina Balaguer-Fernández, Alicia López-Castellano","doi":"10.1080/02713683.2025.2472363","DOIUrl":"10.1080/02713683.2025.2472363","url":null,"abstract":"<p><strong>Purpose: </strong>Herpes zoster ophthalmicus, a manifestation of the varicella-zoster virus in the eye, presents significant clinical challenges. As there is no direct ocular treatment for this infection, new therapeutic options should be studied. Famciclovir, a widely used antiviral drug for herpes zoster, offers higher bioavailability than acyclovir (a common herpes zoster treatment). However, this drug is currently limited to oral dosage forms, which are associated with inherent limitations such as variable absorption or gastrointestinal side effects. This study aimed to formulate new famciclovir eye drops for controlled drug release and targeted delivery to the cornea and sclera.</p><p><strong>Method: </strong>Eye drop formulations containing various polymers (polyvinyl alcohol, hydroxypropyl methyl cellulose and chitosan) were developed and evaluated for physicochemical properties, irritation index and stability. The best performing formulation was used in <i>ex-vivo</i> diffusion and retention studies with rabbit cornea and sclera. The results were analyzed with a new HPLC validation method.</p><p><strong>Results: </strong>The formulation with polyvinyl alcohol exhibited superior properties regarding transparency, turbidity and absence of bubbles. It maintained a physiological pH over time and had an appropriate viscosity of 19.97 ± 0.25 mPa<b>^.</b>s. Non-irritancy was confirmed by the HET-CAM test, and the formulation was stable at room temperature (25 °C), fridge (4 °C) and freezer (-80 °C). <i>Ex- vivo</i> diffusion studies revealed higher diffusion through the sclera compared to the cornea, with greater drug accumulation in sclera.</p><p><strong>Conclusions: </strong>These findings suggest that famciclovir eye drops may offer a viable treatment for ocular herpes zoster infections. Famciclovir also diffuses and retains in targeted membranes, possibly enhancing its therapeutic effects.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"590-599"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tear-Driven Phase Transition Microemulsion for Ocular Delivery of Dexamethasone in the Effective Treatment of Uveitis.","authors":"Nivedita Gautam, Priya Sharma, Neelima Yadav, Jothimani Rajeswari, Karthikeyan Kesavan","doi":"10.1080/02713683.2025.2469243","DOIUrl":"10.1080/02713683.2025.2469243","url":null,"abstract":"<p><strong>Purpose: </strong>The goal of this study was to develop dexamethasone-loaded tear-driven phase transition microemulsions (PTMEs) to effectively treat uveitis.</p><p><strong>Methods: </strong>PTMEs were prepared using the oil titration method. Physicochemical parameters, <i>in vitro</i> release, and ocular irritation studies were performed. The <i>in vivo</i> study, total cell count, and total protein content were estimated on the rabbit eye model.</p><p><strong>Results: </strong>The study revealed that developed PTMEs had nanoglobule sizes, acceptable physicochemical properties, and prolonged drug release. Ex-vivo and in-vivo studies concluded that higher permeability and improved anti-inflammatory properties were observed for PTMEs compared to marketed formulation.</p><p><strong>Conclusion: </strong>The prepared PTMEs showed a sustained release pattern and enhanced therapeutic effectiveness, making them a promising alternative to conventional eye drops for treating uveitis.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"631-640"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-Analysis of the Association Between <i>VEGF</i>-2578C/A Polymorphism and Susceptibility to Type 2 Diabetic Retinopathy.","authors":"Dingyong Wu, Wanting Li","doi":"10.1080/02713683.2025.2451616","DOIUrl":"10.1080/02713683.2025.2451616","url":null,"abstract":"<p><strong>Purpose: </strong>to investigate the association between vascular endothelial growth factor (<i>VEGF)</i>-2578C/A polymorphism and susceptibility to type 2 diabetic retinopathy (T2DR) by meta-analysis.</p><p><strong>Methods: </strong>According to the search strategy, Four databases were retrieved to identify the literature on the relationship between <i>VEGF</i> polymorphism and the risk of T2DR from inception to July 2024. Stata 15.0 was used for data processing.</p><p><strong>Results: </strong>Ten articles were involved in this review, covering 1390 cases and 1306 controls. The pooled results exhibited that the risk of T2DR was associated with <i>VEGF</i>-2578C/A polymorphism under the allele model (A/C: OR= 1.33, 95%CI: 1.04-1.72, <i>p</i> = 0.025) and dominant models (AA+CA/CC: OR= 1.38, 95%CI: 1.00-1.91, <i>p</i> = 0.047). However, in recessive, homozygous, and heterozygous models, no significant difference was observed (all <i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>The <i>VEGF</i>-2578C/A polymorphism is associated with susceptibility to T2DR. In particular, allele A and genotype AA+CA at the <i>VEGF</i>-2578C/A locus were significantly associated with an increased risk of T2DR.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"559-566"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Effects of Timolol, Brinzolamide, Brimonidine and Netarsudil in a Rabbit Model of Water Loading-Induced Ocular Hypertension.","authors":"Junya Maruoka, Yasuhiro Takeji, Daisuke Yamashita, Keisuke Hino, Shinsaku Itoh, Yusuke Kakumoto, Kazushi Sakurai, Hiroki Urashima","doi":"10.1080/02713683.2025.2472365","DOIUrl":"10.1080/02713683.2025.2472365","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to compare the intraocular pressure (IOP)-lowering effects of timolol (a β-blocker), brinzolamide (a carbonic anhydrase inhibitor), brimonidine (an α2-agonist) and netarsudil (a rho kinase inhibitor) in rabbits.</p><p><strong>Methods: </strong>The experiments were performed on 52 female New Zealand white rabbits. The IOP was measured in normotensive rabbits and in water loading-induced ocular hypertension model rabbits. Thirty microliters of timolol, brinzolamide, brimonidine, netarsudil or saline was administered topically to the randomly chosen eye. Ocular hypertension was induced by the oral administration of 60 mL/kg of tap water.</p><p><strong>Results: </strong>In normotensive rabbits, the maximum IOP-lowering effects of timolol, brinzolamide, brimonidine, and netarsudil were 2.9 mmHg (2 h), 5.2 mmHg (1 h), 5.7 mmHg (2 h), and 3.3 mmHg (4 h), respectively. In water loading-induced ocular hypertension model rabbits, the maximum IOP-lowering effects of timolol, brinzolamide, brimonidine, and netarsudil were 3.6, 5.0, 12.2, and 5.0 mmHg, respectively. The IOP-lowering effects of brimonidine and netarsudil were sustained until 90 min after water loading.</p><p><strong>Conclusion: </strong>This study showed that timolol, brinzolamide, brimonidine and netarsudil have IOP-lowering effects in normotensive rabbits and in water loading-induced ocular hypertension model rabbits. In an ocular hypertension rabbit model, brimonidine and netarsudil, which promote aqueous humor outflow, exhibited stronger IOP-lowering effects than timolol and brinzolamide, which suppress aqueous humor production. These results could provide data for characterizing each medication. These data may aid in the development of new glaucoma medications through the combination of existing medications.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"618-623"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Objective Quality Assessment of the Capsulorhexis - A Novel Augmented Reality Tool for Trainees and Experts.","authors":"Daniel Rudolf Muth, Pauline Scherm, Federico Acosta, Miroslav Brêzik, Fernando Benito, Martin Dirisamer, Siegfried Georg Priglinger, Frank Blaser, Sandrine Anne Zweifel, Wolfgang Johann Mayer","doi":"10.1080/02713683.2025.2504569","DOIUrl":"10.1080/02713683.2025.2504569","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the usability of a smartphone-based augmented reality tool for capsulorhexis training.</p><p><strong>Methods: </strong>This is a single-center, prospective, clinical study conducted at the Department of Ophthalmology, University Hospital, LMU Munich, Munich, Germany. Surgery-naïve residents were included and divided into three subgroups: (1) augmented reality supported by the Custom Surgical MicroREC system during their capsulorhexis procedures; (2) Expert support supported by an experienced cataract surgeon; (3) No support without support. Capsulorhexis quality was graded by circularity, centration, and radius.</p><p><strong>Results: </strong>Twenty-one (<i>n</i>_total = 21) participants were enrolled. The augmented reality group was statistically significantly better in terms of circularity than the Expert support (Mann-Whitney's <i>U</i>-test: <i>p</i> = .00175) and the No support group (Mann-Whitney's <i>U</i>-test: <i>p</i> = .00268). The procedure duration decreased statistically significantly for all three groups when try 1 and 10 were compared (augmented reality: <i>p</i> < .0001; Expert support: <i>p</i> = .0099; No support: <i>p</i> < .0001). The other parameters did not differ statistically significantly. The questionnaires handed to the augmented reality and No support group before and after the wet lab revealed a high importance of wet lab trainings to all polled participants. The provided wet lab setup was considered adequate by both groups.</p><p><strong>Conclusion: </strong>Augmented reality can assist in cataract training. The Custom Surgical MicroREC system can have a positive influence on capsulorhexis quality in a training environment. Automated quality metrics measured by the augmented reality software provide an objective quality score of the performed surgical procedure. As the tested augmented reality system can be used in training as well as in a real-world setting, this will be a useful tool for trainees and expert surgeons alike.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Vesicles of Tears and Ocular Surface: An Enigma.","authors":"Asra Fatima, Jyothi Attem, Sandhya Esam, Geeta K Vemuganti","doi":"10.1080/02713683.2025.2503214","DOIUrl":"https://doi.org/10.1080/02713683.2025.2503214","url":null,"abstract":"<p><strong>Purpose: </strong>A stable ocular surface is crucial for maintaining ocular health by protecting against various infections. This is achieved by coordinated function of ocular structures (cornea, limbus, conjunctiva), innervation, and the tear film which forms a protective barrier over the ocular surface ensuring proper hydration, lubrication, and overall ocular comfort. This complex three-layered tear film secreted by different sources ensures its stability by adhesion to the corneal epithelium. Ocular surface fluid kinetics and tear secretion involve complex processes influenced by neural regulation, environmental factors, and molecular composition. Recent advances in cell biology and secretome has unravelled the mysteries of cellular cargo of almost every cell and system i.e. the extracellular vesicles (EVs) which facilitate intercellular communication. EVs are of different sizes, amongst which small EVs (sEVs) potentially are more informative than other EVs.</p><p><strong>Methods: </strong>An extensive review of literature on sEVs in tears and ocular surface was conducted.</p><p><strong>Results: </strong>Emerging literature on sEVs derived from ocular surface structures such as cornea and limbal stem cells contribute to corneal wound healing, regeneration and reduced fibrosis by the activation of specific proteins. A recent study documents that homeostasis between cornea and conjunctiva is maintained by the expression of specific genes triggering <i>trans</i> differentiation in diseased conditions. There is also mounting evidence on role of tear-derived sEVs in normal and diseased states. The approach in which tear layers secreted from three different sources form into a single tri-layered stable biofilm covering the entire ocular surface remains elusive. Hence not surprisingly, the tear sEVs therefore have been referred to as one entity and not attributed to any of the 3 different sources that they originate from.</p><p><strong>Conclusion: </strong>This review attempts to present the recent concepts of sEVs, ocular surface, tears and highlight the gaps in our understanding of tear-derived exosomes and its potential role in homeostasis and disease conditions.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-15"},"PeriodicalIF":1.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical Netarsudil in Childhood Glaucoma: A Systematic Review.","authors":"Omar Shaikh, Lana Kuziez, Osamah J Saeedi, Javaneh Abbasian, Abdelrahman M Elhusseiny","doi":"10.1080/02713683.2024.2445622","DOIUrl":"10.1080/02713683.2024.2445622","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effectiveness and safety of topical netarsudil 0.02% in managing childhood glaucoma.</p><p><strong>Methods: </strong>A literature search in the electronic databases of PubMed CENTRAL, Google Scholar, EMBASE, the Register of Controlled Trials, and Ovid MEDLINE from January 2017 to August 2023 using one or a combination of the following terms: \"netarsudil,\" \"rhopressa,\" \"Rho-kinase,\" \"pediatric glaucoma,\" \"childhood glaucoma,\" \"intraocular pressure\" was conducted.</p><p><strong>Results: </strong>Eight publications (four retrospective studies, one prospective study, and three case reports) were identified evaluating the outcomes of topical netarsudil in childhood glaucoma. Six publications were conducted in the United States, and two publications were conducted in India. Studies included a heterogeneous cohort of primary and secondary childhood glaucoma with a variable range of follow-up (1 week-26 months). The mean IOP reduction after the initiation of topical netarsudil 0.02% in childhood glaucoma patients varies from 0.8 ± 13.2 to 12.0 ± 0.0 mmHg. The most common ocular adverse event was conjunctival hyperemia, seen in 27 out of 82 eyes (32.9%), followed by corneal honeycombing/reticular epithelial edema, seen in 13 out of 82 eyes (15.9%).</p><p><strong>Conclusion: </strong>Limited literature is currently available on using topical netarsudil in childhood glaucoma. However, in children with refractory glaucoma on maximum topical medications, netarsudil may serve as an adjunctive treatment option, potentially delaying the need for further surgical interventions in some patients. Careful corneal examination is needed before and after initiation of netarsudil treatment for early detection of corneal adverse events that may compromise the vision.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"461-466"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142920794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Retinal Segmentation Metrics with Machine Learning for Predictions from Mouse SD-OCT Scans.","authors":"Maide Gözde İnam, Onur İnam, Xiangjun Yang, Qun Zeng, Gülgün Tezel","doi":"10.1080/02713683.2025.2456783","DOIUrl":"10.1080/02713683.2025.2456783","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to initially test whether machine learning approaches could categorically predict two simple biological features, mouse age and mouse species, using the retinal segmentation metrics.</p><p><strong>Methods: </strong>The retinal layer thickness data obtained from C57BL/6 and DBA/2J mice were processed for machine learning after segmenting mouse retinal SD-OCT scans. Twenty-two models were trained to predict the mouse groups. The best neural network model was optimized for better outcomes. Prediction accuracy, the area under the curve, sensitivity, specificity, precision, and F-1 score values were obtained.</p><p><strong>Results: </strong>The Wilcoxon Signed-Rank test provided significantly higher validation accuracy for neural networks than decision trees, discriminant analysis, support vector machines, and k-nearest neighbor classifiers (<i>p</i> = 0.005 for all). For C57BL/6-DBA/2J classification, a mean validation accuracy of 88.11 ± 3.92% (95% CI: 86.99-89.22) was achieved for the neural network when the optimized neural network had 92.31% final test accuracy with an area under the curve value of 0.9762, 94.44% sensitivity, 90.48% specificity, 89.47% precision, and 0.92 F-1 score. The optimized neural network model for age group differentiation had a final test accuracy of 82.05% with a 0.9064 area under the curve value, 77.27% sensitivity, 88.24% specificity, 89.47% precision, and 0.83 F-1 score.</p><p><strong>Conclusions: </strong>These findings validate that machine learning, using segmentation metrics instead of images, can effectively analyze retinal OCT scans in mice for categorical predictions in experimental models. Expanding this approach with additional features, including histopathological and functional correlations, is expected to improve the prediction power further, promising valuable applications to predict more complex outcomes in experimental and clinical studies.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"502-511"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}