Bufalin Regulates STAT3 Signaling Pathway to Inhibit Corneal Neovascularization and Fibrosis After Alkali Burn in Rats.

IF 1.7 4区医学 Q3 OPHTHALMOLOGY

Current Eye Research Pub Date : 2024-10-02 DOI:10.1080/02713683.2024.2408392

Chao Wu, Lu Shi, Yan Deng, Hongping Chen, Ying Lu, Xiaoyan Xiong, Xiaolong Yin

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引用次数: 0

Abstract

Purpose: Bufalin (BU) is a bioactive ingredient extracted from the skin and parotid venom glands of Bufo raddei, which can effectively inhibit angiogenesis. The aim of this study was to investigate whether BU could affect corneal neovascularization (CoNV).

Methods: A rat CoNV model (right eye) was constructed by administration of NaOH, and the left eye served as a control. Corneal damage scores of rats were detected. Hematoxylin & eosin, TUNEL, and Masson staining examined pathological changes, apoptosis, and fibrosis of corneal tissues. Immunohistochemistry and western blotting assessed the expression of proteins.

Results: BU intervention resulted in a significant reduction in corneal inflammatory cells, repair of corneal epithelial hyperplasia, significant reduction in stromal edema, and reduction in vascular proliferation. BU can inhibit corneal neovascularization.

Conclusion: This study demonstrated that BU inhibits CoNV, fibrosis, and inflammation by modulating the STAT3 signaling pathway, elucidating the intrinsic mechanism of its protective effect. BU has great potential in the treatment of CoNV caused by corneal alkali burns.

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布法林调控 STAT3 信号通路，抑制大鼠碱烧伤后角膜新生血管形成和纤维化

目的：Bufalin（BU）是从蟾蜍皮肤和腮腺毒液中提取的一种生物活性成分，能有效抑制血管生成。本研究旨在探讨 BU 能否影响角膜新生血管（CoNV）：方法：用 NaOH 建立大鼠 CoNV 模型（右眼），左眼作为对照。检测大鼠角膜损伤评分。血红素和伊红、TUNEL 和 Masson 染色检查角膜组织的病理变化、凋亡和纤维化。免疫组织化学法和免疫印迹法评估了蛋白质的表达：结果：BU干预后，角膜炎症细胞明显减少，角膜上皮增生得到修复，基质水肿明显减轻，血管增生减少。结论：BU 可抑制角膜新生血管：本研究表明，BU通过调节STAT3信号通路抑制CoNV、纤维化和炎症，阐明了其保护作用的内在机制。BU在治疗角膜碱烧伤引起的CoNV方面具有巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Eye Research 医学-眼科学

CiteScore

4.60

自引率

0.00%

发文量

163

审稿时长

12 months

期刊介绍： The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.