Current Eye Research最新文献

{"title":"<i>Bacillus Coagulans</i> Secretes Extracellular Vesicles and Modulates the Proliferation of Conjunctival Epithelial Cells via the P53/CDKN1A Signaling Pathway.","authors":"Jingyu Zhang, Ruiming Yang, Jiamian Liang, Qiudan Huang, Amy Michelle Huang, Wangjian Qiu, Fang Li, Li Wang, Yue Liang, Zhiping Liu","doi":"10.1080/02713683.2025.2513556","DOIUrl":"https://doi.org/10.1080/02713683.2025.2513556","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the microbial strains in pterygium patients' tear samples <i>via</i> 16S rRNA sequencing and evaluate their impact on human conjunctival epithelial cells (HConEpics) and pterygium fibroblasts (HPFs) proliferation.</p><p><strong>Methods: </strong>Tear samples were cultured aerobically and anaerobically on blood agar plates. Bacterial colonies were characterized by 16S rRNA sequencing. Proliferation of HConEpics and HPFs was assessed using CCK-8 and EDU assays. The role of bacterial extracellular vesicles (EVs) was explored using the exosome inhibitor GW4869. EVs were isolated and characterized by transmission electron microscopy and nanoparticle tracking analysis (NTA), with DIL staining confirming their internalization by host cells. Transcriptomic sequencing and the SIRT2-IN-11 inhibitor were used to elucidate the molecular mechanisms and regulatory pathways.</p><p><strong>Results: </strong>16S rRNA analysis revealed a significant reduction in the concentration of <i>Bacillus coagulans</i> (BC) in pterygium patients. BC significantly promoted HConEpics proliferation while inhibiting HPFs proliferation. GW4869 significantly reduced the stimulatory effect of BC culture supernatant on HConEpics proliferation, confirming EVs-medicated regulation. BC-derived EVs, isolated by ultracentrifugation, were internalized by HConEpics, promoting proliferation and inducing G1 phase cell cycle accumulation. These EVs also inhibited TGF-β-induced damage to HConEpics. Transcriptomic sequencing identified the p53 pathway as a key regulatory pathway, further clarified by SIRT2-IN-11.</p><p><strong>Conclusions: </strong>This study offers novel insights into pterygium pathogenesis and identifies potential therapeutic targets.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of Compensation Algorithm in Healthy Volunteers Using Spectral Domain Optical Coherence Tomography Angiography.","authors":"Giulia Corradetti, Zhongdi Chu, Louay Almidani, Federico Corvi, Ruikang K Wang, SriniVas R Sadda","doi":"10.1080/02713683.2025.2512581","DOIUrl":"https://doi.org/10.1080/02713683.2025.2512581","url":null,"abstract":"<p><strong>Purpose: </strong>To generate and validate a spectral domain optical coherence tomography angiography (SD-OCTA) device-specific algorithm to compensate for signal attenuation in choriocapillaris (CC) en face OCTA slabs.</p><p><strong>Methods: </strong>Healthy subjects were imaged on SD-OCTA with a 6 × 6 mm pattern at the Doheny-UCLA Eye Centers. The CC slabs were segmented at the level of the Bruch's membrane using a 16-μm thick slab with the inner boundary located 4 μm below the Bruch's membrane. The CC slabs were exported and normalized. The percentage of CC flow deficits (CC FD %) was computed on binarized slabs before and after the application of an SD-OCTA specific compensation algorithm. On the same cohort, we also tested the compensation algorithm previously developed for SS-OCTA CC slabs.</p><p><strong>Results: </strong>Twenty-eight eyes from 28 subjects were included in the analysis. The mean (SD) CC FD % was 13.15 (3.91) for the normalized and compensated (SD-OCTA specific) images and 13.73 (4.20) for the normalized and uncompensated images (<i>p</i> = 0.1). The compensated and uncompensated CC FD were strongly correlated (<i>p</i> < 0.001). Bland-Altman plots demonstrated good agreement between the compensated and uncompensated CC slabs for the quantification of CC FD. When applying the SS-OCTA compensation algorithm on the SD-OCTA CC slabs, there was a statistically significant difference between CC FD% measured on the compensated and uncompensated CC slabs (<i>p</i> < 0.0001).</p><p><strong>Conclusions: </strong>An SD-OCTA specific compensation algorithm can be applied which does not cause the loss of artifactual FD in healthy eyes, with similar CC FD% values in compensated and uncompensated images.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pneumatic Trabeculectomy: A Modification in Conventional Trabeculectomy.","authors":"Shikha Gupta, Monika Arora, Karthikeyan Mahalingam, Viney Gupta, Pradeep Venkatesh","doi":"10.1080/02713683.2025.2513554","DOIUrl":"https://doi.org/10.1080/02713683.2025.2513554","url":null,"abstract":"<p><strong>Purpose: </strong>We developed a novel technique called \"pneumatic trabeculectomy\"(PTB) which uses constant pressure air infusion <i>via</i> the Constellation Vision System (Alcon. Laboratories, Inc., Fort Worth, TX, USA) system to prevent anterior chamber collapse/intraocular pressure fluctuations during trabeculectomy in high-risk cases. In this study, we compared the efficacy and safety of PTB with conventional trabeculectomy surgery.</p><p><strong>Methods: </strong>In this prospective, interventional, nonrandomized, comparative pilot study, certain high-risk cases planned for trabeculectomy were assigned to Group A (conventional trabeculectomy) or Group B (pneumatic trabeculectomy). The primary outcome measure was to assess the anterior chamber (AC) fluctuations (through assessing anterior chamber shallowing/collapse), the frequency of need to form AC, and the occurrence of any intra-operative/early postoperative complications.</p><p><strong>Results: </strong>Of the 48 eligible participants, 24 were assigned to Group A and Group B. The mean surgical time required to complete the surgery was 17.63 ± 1.44 min in group A and 17.21 ± 1.50 min in group B (<i>p</i> = 0.83). In group A, 83.3% (20/24)eyes, anterior chamber collapsed during the step of scleral osteotomy/iridectomy compared to 4.17% (1/24) eyes in group B (<i>p</i> < 0.001) (Fischer's test). The anterior chamber remained stable throughout the surgery in Group B, while AC formation was required at least twice (mean 2.3 ± 0.7) in eyes undergoing conventional trabeculectomy in group A (<i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>PTB in high-risk cases had a significantly lower incidence of intra-operative anterior chamber collapse, requiring less frequent AC reformations, making it a safer alternative to conventional trabeculectomy.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.7,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects and Molecular Mechanisms of KD025 in Intraocular Pressure Regulation.","authors":"Cong Guo, Yalong Dang","doi":"10.1080/02713683.2025.2512622","DOIUrl":"https://doi.org/10.1080/02713683.2025.2512622","url":null,"abstract":"<p><strong>Aim: </strong>To quantitatively evaluate the IOP-lowering efficacy of topical KD025 in normotensive and steroid-induced ocular hypertensive rat models, and to elucidate its mechanisms concerning TM cytoskeletal remodeling, fibrotic modulation, and cell behavior using primary human TM cells.</p><p><strong>Methods: </strong>Normotensive and steroid-induced (dexamethasone) ocular hypertensive Sprague-Dawley rats received topical KD025; IOP was monitored using rebound tonometry. TM morphology was assessed by H&E staining. In primary human TM cells, functional effects (wound healing) and the expression of α-smooth muscle actin (α-SMA), fibronectin (FN), F-actin, and myocilin (a steroid-response marker) were analyzed <i>via</i> immunofluorescence and Western blotting following KD025 and/or dexamethasone treatment.</p><p><strong>Results: </strong>KD025 significantly reduced IOP in normotensive rats, with peak effects at 6 h post-administration (though no clear dose-dependency was observed between 10-25 µM). In steroid-induced hypertensive rats, 20 µM KD025 achieved significantly greater IOP reduction versus vehicle (<i>p</i> < 0.01). Histological analysis suggested potential TM structural relaxation. <i>In vitro</i>, KD025 significantly inhibited TM cell migration and downregulated dexamethasone-induced α-SMA expression. Paradoxically, and differing from typical pan-ROCK inhibitor effects, KD025 treatment increased total cellular FN protein (<i>p</i> < 0.01 vs DEX alone) and further exacerbated dexamethasone-induced F-actin protein levels (<i>p</i> < 0.05 vs DEX alone).</p><p><strong>Conclusions: </strong>KD025 demonstrates effective IOP-lowering capabilities in rodent models. Its mechanism likely involves modulating TM cell contractility (<i>via</i> α-SMA reduction) and inhibiting cell migration. The unexpected findings on fibronectin and F-actin suggest that selective ROCK2 inhibition by KD025 induces complex and distinct effects on TM extracellular matrix dynamics and cytoskeletal organization compared to non-selective ROCK inhibitors. These results highlight ROCK2 as a promising therapeutic target for glaucoma, though its distinct cellular actions warrant further investigation.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tear Global Metabolomic Differences in Pre-Operative Cataract Patients With and Without Dry Eye Disease.","authors":"Morten Gundersen, Christian Nilsen, Per Jensen, Mazyar Yazdani, Øygunn Utheim, Elise Sandås Sand, Helge Rootwelt, Kjell Gunnar Gundersen, Katja Benedikte Prestø Elgstøen","doi":"10.1080/02713683.2025.2472369","DOIUrl":"10.1080/02713683.2025.2472369","url":null,"abstract":"<p><strong>Purpose: </strong>The primary aim of this article was to investigate differences in the metabolomic profile of tear fluid obtained from pre-operative cataract patients, with or without dry eye disease. The objective was to look for metabolomic signatures that might discriminate between the two groups.</p><p><strong>Methods: </strong>A total of 222 patients were enrolled in the study. Eighty-one were randomly selected for metabolomic analysis from both dry eye positive and dry eye negative groups, categorized prior to cataract surgery. Tear film was collected using Schirmer-1 strips and analyzed using an optimized method developed for low-volume Schirmer samples and allowing for repeated analyses, including other -omics approaches at a later stage. Metabolomic data were collected using a global liquid chromatography-mass spectrometry method. Samples were compared using principal component analysis and volcano plots to look for overall global differences as well as group-specific metabolic signatures.</p><p><strong>Results: </strong>All samples were analyzed with a high number of features identified. No group-specific clustering was observed in principal component analysis for the dry eye positive or dry eye negative groups. However, volcano plots revealed that a majority of the metabolomic features had lower concentration in the dry eye positive group compared to the dry eye negative group. Four of these features had a Log₂-fold change ≤ -1 and <i>p</i> value ≤.05. These warrant further study.</p><p><strong>Conclusion: </strong>Although no overall global difference was observed on the principal component analysis plots, a general trend of lower metabolite concentrations in the dry eye disease group was shown. Moreover, several metabolites of interest were discovered with significantly different signal intensities between the groups. These metabolites may aid future diagnostics and serve as possible biomarkers and therapeutic targets for dry eye disease in pre-operative cataract patients.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"579-589"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Cyclosporine Eye Drop Use on the Success of Punctoplasty in Acquired Punctal Stenosis.","authors":"Melek Mutlu Sönmez, Elif Ertan Baydemir","doi":"10.1080/02713683.2025.2466659","DOIUrl":"10.1080/02713683.2025.2466659","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of cyclosporine-A (CsA) 0.05% eye drops for management of acquired punctal stenosis, either alone or in combination with punctoplasty.</p><p><strong>Methods: </strong>In this prospective study patients referred to the oculoplastics clinic with acquired punctal stenosis (APS) were divided into three groups. Group A consisted of patients treated with CsA, Group B consisted of patients who underwent punctoplasty, and Group C included patients treated with both CsA and punctoplasty. Munk score, fluorescein dye disappearence test (FDDT), punctal opening grading were evaluated at baseline, and at the 1st and 6th month follow-ups.</p><p><strong>Results: </strong>One hundred-eleven eyes of 80 patients were included in the study. While the punctum grade was significantly higher, the FDDT grade and Munk scores were significantly lower in group C compared to groups A and B at the 1st and 6th month follow-ups. The functional success rate was 69%, 79%, and 88% (<i>p</i> = .01), and the anatomical success rate was 77%, 77%, and 90% (<i>p</i> = .03) in Group A, B, and C, respectively, at the 6th month follow-up.</p><p><strong>Conclusions: </strong>In the treatment of APS, the use of CsA alone and the punctoplasty procedure demonstrated comparable anatomical and functional success rates at the 6th month follow-up. However, combining CsA with punctoplasty enhances both anatomical and functional success rates.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"651-655"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function of AMPK/mTOR Signaling in TGF-β1-Induced Pterygium Fibroblast Proliferation and Transdifferentiation.","authors":"Yun Hua, Xinrong Zhao, Na Tang, Huijuan Wan, Haidong Lian, Ting Yuan, Chao Si","doi":"10.1080/02713683.2025.2470410","DOIUrl":"10.1080/02713683.2025.2470410","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the regulatory role of the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin (mTOR) signaling pathway in mediating transforming growth factor-beta 1 (TGF-β1)-induced cellular proliferation and transdifferentiation processes in human pterygium fibroblasts (HPFs).</p><p><strong>Methods: </strong>HPFs were stimulated with TGF-β1 <i>in vitro</i>. Cell viability was assessed using the CCK-8 assay at 12/24/48-h post-stimulation, while migratory capacity was evaluated through standardized wound healing assays. Quantitative real-time PCR (qPCR) and western blotting analyses were employed to evaluate the expression of proliferation marker proliferating cell nuclear antigen (PCNA) and myofibroblast transdifferentiation biomarker α-smooth muscle actin (α-SMA). Western blotting further characterized the activation status of AMPK/mTOR signaling by quantifying phosphorylated AMPK (p-AMPK) and phosphorylated mTOR (p-mTOR), with total AMPK and mTOR levels serving as loading controls. To establish mechanistic causality, TGF-β1-primed HPFs were modulated using the AMPK inhibitor Compound C and activator AICAR for 24 h. Functional consequences were analyzed through CCK-8 viability assays and wound healing assays, while molecular correlates were assessed <i>via</i> qPCR and western blotting for PCNA, α-SMA, and pathway components. This comprehensive approach delineated the AMPK/mTOR axis as a critical regulator of TGF-β1-driven fibrotic phenotype acquisition in HPFs.</p><p><strong>Results: </strong>Following TGF-β1 pretreatment-induced activation of human HPFs, both cell viability and migratory capacity were markedly enhanced, with concomitant upregulation of PCNA and α-SMA. Compound C-mediated AMPK inhibition potentiated the TGF-β1-induced enhancements in HPFs viability and migration rate, concomitant with reduced p-AMPK/AMPK ratio and elevated expression of PCNA, α-SMA, and p-mTOR/mTOR ratio. Conversely, AICAR-driven AMPK activation attenuated TGF-β1-stimulated effects, demonstrating diminished viability, suppressed migratory capacity, increased p-AMPK/AMPK ratio, and decreased expression of PCNA, α-SMA, and p-mTOR/mTOR ratio.</p><p><strong>Conclusions: </strong>This study demonstrates the critical regulatory role of the AMPK/mTOR signaling pathway in controlling TGF-β1-induced proliferation and transdifferentiation in HPFs, thereby providing a potential mechanistic framework for developing novel therapeutic interventions targeting fibrotic ocular surface disorders.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"600-609"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choriocapillaris Density Remains Constant in Healthy Older Adults After 24 Weeks of High-Speed Circuit Training.","authors":"Kevin K Zhou, Ava-Gaye Simms, Alessandro Gregori, Rosalia Parrino, Abdulrahman Mamoon Allaf, Joseph F Signorile, Jianhua Wang, Hong Jiang","doi":"10.1080/02713683.2025.2469226","DOIUrl":"10.1080/02713683.2025.2469226","url":null,"abstract":"<p><strong>Purpose: </strong>The goal of this study was to assess the changes in choriocapillaris perfusion density (CCD) after 24 weeks of structured high-speed circuit resistance training (HSCT) in healthy older adults.</p><p><strong>Methods: </strong>A total of 30 healthy, cognitively normal adults, 60 years old and above, were recruited for the study. The participants were randomized to either the HSCT group (<i>n</i> = 15) or the control group (CON, <i>n</i> = 15). The HSCT group trained 3 times a week on non-consecutive days for 24 weeks, while the CON group did not perform formal training. Optical coherence tomography angiography (OCTA) was used to image both eyes of each study participant at baseline and 24-week follow-up. The follow-up session was completed within 3 days of the last exercise session for the HSCT group. The CCD was measured within a 2.5-mm in diameter centered on the fovea.</p><p><strong>Results: </strong>There were no significant differences in CCD between baseline and 24-week follow-up in either the HSCT group or the CON group. In the HSCT group, the CCD was 62.6 ± 6.1% (mean ± SD) at baseline and 63.7 ± 6.1% at the 24-week follow-up (<i>p</i> = 0.21). In the CON group, the CCD was 62.8 ± 5.6% at baseline and 63.8 ± 4.9% at 24 weeks (<i>p</i> = 0.18).</p><p><strong>Conclusion: </strong>CCD was unchanged after a 24-week high-speed circuit resistance training in healthy older individuals, suggesting possible regulatory maintenance of choroidal blood flow. Further research with multiple age cohorts may be necessary to corroborate these findings.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"624-630"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Pupillary Responses in Healthy Eyes and Eyes Implanted with Multifocal or Monofocal Intraocular Lenses.","authors":"Kohei Miyata, Shinichiro Kobayakawa","doi":"10.1080/02713683.2025.2469833","DOIUrl":"10.1080/02713683.2025.2469833","url":null,"abstract":"<p><strong>Purpose: </strong>Comparison of dynamic pupillary parameters (DPPs) between healthy volunteers and multi- or monofocal intraocular lens (IOL) implanted eyes, and examination of the correlation between postoperative visual acuity (VA), contrast sensitivity (CS), visual dysphotopsias (VD), and these parameters in IOL-implanted eyes.</p><p><strong>Methods: </strong>This cross-sectional study classified and analyzed DPPs from healthy volunteers (308 eyes) according to age. In the multifocal IOL group (119 eyes), 51 eyes were implanted with Lentis Comfort^® (L group), 32 with PanOptix^® (P group), and 36 with Synergy^® (S group). The monofocal IOL group included 33 eyes implanted with Vivinex Impress^® (V group). DPPs by the pupillometer (PLR-3000), distance, intermediate and near VA, and CS and VD were examined one month postoperatively in 152 eyes.</p><p><strong>Results: </strong>Significant decreases in maximum or minimum pupillary diameter (INIT, END), percentage of pupillary constriction (DELTA), latency between light stimulation and constriction (LAT), and average and maximum constriction velocity (ACV, MCV), average dilation velocity (ADV), were observed with aging in the healthy eyes group. The preoperative group showed a significant age-related decrease in DELTA and increase in LAT. The postoperative group showed a significant age-related decrease in INIT, END, and ADV. The L group exhibited a significant positive correlation between ADV and uncorrected intermediate VA (UIVA), while the P group exhibited a significant positive correlation between ACV and UIVA, and the S group exhibited a significant negative correlation between ACV and uncorrected near VA. For CS, the S group exhibited a significant positive correlation with ADV at 1.0 and 0.7 degrees, and the P group exhibited a significant negative correlation between ACV and glare.</p><p><strong>Conclusion: </strong>DPPs are greatly affected by age. DPPs for multi- or monofocal IOL eyes correlate with uncorrected VA, CS, and VD.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"610-617"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Conjunctival Cell HLA-DR Expression with the Severity of Signs and Symptoms of Dry Eye Disease at Baseline in the NORTHERN LIGHTS Phase 2 Trial.","authors":"Eric J Kuklinski, Neeta S Roy, Morten Præstegaard, Yi Wei, Penny A Asbell","doi":"10.1080/02713683.2025.2469254","DOIUrl":"10.1080/02713683.2025.2469254","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the association of HLA-DR on ocular surface conjunctival cells with the severity of signs and symptoms of dry eye disease (DED) in patients enrolled in the NORTHERN LIGHTS phase 2 trial.</p><p><strong>Methods: </strong>Patients with moderate to severe DED were recruited from 10 different countries in Europe. Symptoms of DED were assessed using the visual analog scale (VAS) global discomfort score, and signs <i>via</i> corneal fluorescein staining (CFS), lissamine green staining, Schirmer's test, and tear osmolarity. HLA-DR expression was evaluated <i>via</i> flow cytometry from impression cytology of conjunctival cells using the EyePrim™ device sampled from 205 DED patients.</p><p><strong>Results: </strong>HLA-DR expression was detected in a mean of 3.4% of cells at baseline. HLA-DR expression was significantly associated with corneal staining severity at baseline (<i>p</i> < 0.01). HLA-DR expression was not significantly associated with DED symptoms (VAS global discomfort score), nor lissamine green staining, Schirmer's test, tear osmolarity, or Sjogren's syndrome.</p><p><strong>Conclusion: </strong>HLA-DR was present in a wide range of percentages among DED patients indicating the heterogeneous nature of DED. HLA-DR was significantly associated with corneal fluorescein staining suggesting a correlation of the biomarker with ocular surface damage.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"567-571"},"PeriodicalIF":1.7,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}