Current Eye Research最新文献

{"title":"Mitochondrial Dysfunction During TGF-β1-Induced Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells.","authors":"Xinyi Cheng, Xunyi Gu, Fang Wang","doi":"10.1080/02713683.2025.2464783","DOIUrl":"10.1080/02713683.2025.2464783","url":null,"abstract":"<p><strong>Purpose: </strong>Epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells contributes to the epiretinal membrane development in proliferative vitreoretinopathy (PVR). This study aimed at investigating changes in mitochondrial function during EMT in PVR.</p><p><strong>Methods: </strong>Transmission electron microscopy (TEM) was utilized to examine the mitochondrial morphology in human PVR epiretinal membranes and retinal pigment epithelium of human donor eyes. Utilizing TGF-β1 induced EMT in ARPE-19 cells as an <i>in vitro</i> model, we assessed mitochondrial morphology using transmission electron microscopy (TEM), evaluated mitochondrial function through various assays including detection and analysis of mitochondrial membrane potential (MMP), mitochondrial deoxyribonucleic acid (mtDNA), reactive oxygen species (ROS), ATP, oxygen consumption rate (OCR), and extracellular acidification rate (ECAR). RNA sequencing was performed to identify differentially expressed genes (DEGs) related to mitochondrial function and PVR pathogenesis.</p><p><strong>Results: </strong>Mitochondrial morphological damage was observed in human PVR epiretinal membranes. TGF-β1 treatment led to morphological changes in mitochondria, increased oxidative stress, mitochondrial membrane depolarization, and reduction in mtDNA, mitochondrial respiration, and ATP production, indicating mitochondrial dysfunction in EMT ARPE-19 cells. Furthermore, RNA sequencing data highlighted the dysfunction, showing downregulation of mitochondria-related pathways and mitochondrial transcription factor A (TFAM), crucial for mtDNA maintenance.</p><p><strong>Conclusion: </strong>Our findings indicated that TGF-β1 treatment induced mitochondrial dysfunction in RPE cells during EMT, providing insights into the molecular mechanisms of PVR development.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"527-535"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Approach for Suprachoroidal Space Injection: An Experimental Evaluation.","authors":"Jinyi Long, Xue Wu, Ping Hu, Ning Cui, Ling Xu, Yuhong Ye, Hao Chen, Qinghua Peng, Xinmin Lu, Da Long","doi":"10.1080/02713683.2025.2469829","DOIUrl":"10.1080/02713683.2025.2469829","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to describe an innovative suprachoroidal space injection technique using a combination of 30 G and 22 G needles attached to a 1 ml injector. The efficacy and applicability of this technique in suprachoroidal injections are evaluated.</p><p><strong>Methods: </strong>In this study, we conducted both <i>in vitro</i> and <i>in vivo</i> injection experiments using isolated porcine eyes and live SD rats, respectively. The injector needle was inserted into the sclera with the bevelled tip facing the sclera and parallel to the corneal limbus, and the methylene blue solution was injected into the suprachoroidal space. The accuracy of the injection was confirmed by OCT imaging and frozen section microscopy, demonstrating that the dye was successfully delivered to the suprachoroidal space.</p><p><strong>Results: </strong>The suprachoroidal injector successfully injected the solution into the suprachoroidal space of isolated porcine eyes and live rats without entering the vitreous cavity.</p><p><strong>Conclusion: </strong>The 22 G needle demonstrated sufficient rigidity and stability to ensure proper scleral compression, supporting the consistency of the injection depth. The 30 G needle exhibited exceptional sharpness and precision, allowing for more accurate control of the drug dosage. The appropriate force applied to the sclera facilitated the precise depth of injection. The angle of the needle, parallel to the corneal limbus, helped avoid penetration into the vitreous or subretinal space, reducing the risk of complications. This device offers new tools and methods for ophthalmic research and clinical practice, with significant clinical application prospects.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"536-543"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postoperative Use of Pilocarpine After Kahook Dual Blade Goniotomy in Mild to End-Stage Glaucoma.","authors":"Julia L Xia, Dhillon Zaver, Emmeline J Kim, Jennifer L Patnaik, Monica K Ertel, Cara E Capitena, Jeffrey R SooHoo, Malik Y Kahook, Mina B Pantcheva, Leonard K Seibold","doi":"10.1080/02713683.2025.2464795","DOIUrl":"10.1080/02713683.2025.2464795","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to determine if using pilocarpine postoperatively affects clinical outcomes after goniotomy.</p><p><strong>Methods: </strong>Retrospective series comparing 532 Kahook Dual Blade goniotomy (KDB-G) procedures with (P+) or without (P-) pilocarpine use in the postoperative period. This study includes patients with both primary and secondary glaucoma ranging from mild to end-stage, undergoing KDB-G with or without phacoemulsification (phaco). The primary outcome measure was surgical success defined as intraocular pressure (IOP) < 21 mmHg in addition to either a reduction of >20% and/or the reduction of ≥1 topical glaucoma medications. Secondary outcomes were mean IOP, number of medications, and rate of hyphema and IOP spike at one week postoperatively.</p><p><strong>Results: </strong>The success rate was significantly greater in Group P + at postoperative month 1 (<i>p</i> = .02), 3 (<i>p</i> = .01), 12 (<i>p</i> = .048), and 16 (<i>p</i> = .02). The differences in mean IOP (<i>p</i> = .084) and number of medications (<i>p</i> = .15) were not significantly different at one year. However, Group P + required significantly less medications than Group P - at nearly all time points (months 1, 3, 6, 20, and 24). There were no differences in rates of hyphema (<i>p</i> = .30) or postoperative week 1 IOP spikes (<i>p</i> = .66) between groups.</p><p><strong>Conclusion: </strong>The use of pilocarpine postoperatively may improve surgical success and may reduce the number of glaucoma medications needed after goniotomy.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"490-495"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Bench to Bedside: Unraveling Cerebral Malaria and Malarial Retinopathy by Combining Clinical and Pre-Clinical Perspectives.","authors":"Shannon McDonnell, Ian J MacCormick, Kevin Harkin, Reinhold J Medina, Ana Rodriguez, Alan W Stitt","doi":"10.1080/02713683.2025.2463142","DOIUrl":"10.1080/02713683.2025.2463142","url":null,"abstract":"<p><p>Infection with <i>Plasmodium falciparum</i> carries a significant risk of cerebral malaria (CM). Children are particularly susceptible to human CM (HCM) which manifests as an acute neurovascular encephalopathy leading to high levels of mortality. Occurring in parallel with CM, malarial retinopathy (MR) is readily detected on ophthalmoscopy as one or more of: white-centered retinal hemorrhage, retinal whitening, and vessel discoloration. It leads to several distinct types of blood retinal barrier (BRB) breakdown. The precise molecular mechanisms underpinning CM and MR remain ill-defined, but parasitemia is known to drive progressive neurovascular obstruction and inflammation leading to cerebral and retinal edema and ischemia. Extensive clinical studies in patients with CM have shown that retinal examination is a useful approach for understanding pathology and an indicator for risk of mortality and morbidity. Fully understanding the cellular and molecular mechanisms that underpin CM and MR is important for developing new therapeutic approaches and in this regard the murine model of experimental CM (ECM) has proved to offer considerable value. Much is known about brain pathology in this model although much less is understood about the retina. In this review, we seek to evaluate MR in clinical scenarios and make comparisons with the retina from mice with ECM. Through detailed <i>in vivo</i> and post-mortem studies in the mouse and human retina, this review highlights the links between CM and MR and how this will aid our understanding of the disease progression and pathogenesis.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"512-526"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Best Practices for Eye Stroke Vision Rehabilitation: A Qualitative Study.","authors":"Kimberly Hreha, Nathan A Boucher, Brian Mac Grory, April Martina Burch, Emmanuel Acheampong, Heather E Whitson","doi":"10.1080/02713683.2025.2456788","DOIUrl":"10.1080/02713683.2025.2456788","url":null,"abstract":"<p><strong>Purpose: </strong>Central retinal artery occlusion, also known as an eye stroke, results in visual impairment and functional challenges. Our study objectives were to identify meaningful measures and factors that indicate or enable successful recovery after eye stroke and to determine optimal processes to support research, including exploring barriers and facilitators to successful research participation.</p><p><strong>Methods: </strong>We used qualitative methods including the 5Ts Framework (target population identification, team composition, time considerations, tips to accommodate older adults, tools for inclusive enrollment of older adults) to provide a guide to the development of the semi-structured interviews and to help facilitate the research process such as the set-up of interviews. We enrolled three groups: individuals living with the sequelae of eye stroke, care partners, and health care providers. We conducted a descriptive content analysis with an inductive approach.</p><p><strong>Results: </strong>Twenty-five people participated. This group included 10 eye stroke survivors (median age, 62.5; range, 56-84 years; 20% were women), 4 care partners (median age, 57 years; range, 56-59 years; 100% were women), and 11 health care providers (median age, 33 years; 25-60 years; 54.5% were women). Four themes emerged from the data: (1) returning to full independence, (2) family support, (3) strategies for participation, and (4) lack of accessibility.</p><p><strong>Conclusions: </strong>The themes identified by participant groups indicated that strategies such as providing sufficient lighting and incorporating family support are key for research participation by individuals with vision impairment related to eye stroke. Furthermore, it is essential that researchers determine ways to address inequities, including lack of accessibility to research sites. This work has implications for how research teams develop research processes and implement research findings into clinical settings for eye stroke survivors.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"544-550"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the Impact of AI-Assisted Portable Slit Lamps on Rural Primary Ophthalmic Medical Service.","authors":"Sile Yu, Chunhong Yan, Guanghao Qin, Emmanuel Eric Pazo, Xiangdong He, Peng Qi, Mingze Li, Dongming Han, Wei He, Xingru He","doi":"10.1080/02713683.2025.2458131","DOIUrl":"10.1080/02713683.2025.2458131","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effect of an AI-assisted portable slit lamp (iSpector) and basic ophthalmology training on cataract detection, referral, and surgery rate in rural areas.</p><p><strong>Methods: </strong>This randomized control trial randomly assigned 63 village doctors to either the AI-assisted group (providing iSpector and training) or the control group (providing training). Doctors were followed for 1 year before intervention as a baseline and 1 year after to make the comparison. Multivariable Poisson regression was applied to compare the difference in cataract detection, referral, and surgery rate between the two groups, adjusted for primary doctors' baseline characteristics. We further conducted subgroup analysis to estimate the change after the intervention.</p><p><strong>Results: </strong>Compared to the control group, the detection, referral, and surgery rate of cataracts among the AI-assisted group was comparable, 1.7 times higher, and 4.9 times higher, respectively. Providing iSpector and training increased the detection, referral, and surgery rate of cataracts. However, only based on training to elevate the detection rate of cataracts did not change the referral and surgery rate.</p><p><strong>Conclusions: </strong>iSpector helps village doctors detect and refer cataract patients appropriately, thus increasing the probability that patients receive cataract surgery.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"551-558"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Outcomes of Two Lenticule Thickness Types in Descemet Stripping Automated Endothelial Keratoplasty: One-Year Results.","authors":"Neal Rangu, Michael Heath, Michael Szkarlat, Kai Ding, Roya Garakani, Kenan Bakri, Evan Pike, Hooman Harooni, Christopher Chow, Surendar Dwarakanathan, Liam Redden, Kamran M Riaz","doi":"10.1080/02713683.2025.2458152","DOIUrl":"10.1080/02713683.2025.2458152","url":null,"abstract":"<p><strong>Purpose: </strong>To compare clinical outcomes of eyes undergoing Descemet stripping automated endothelial keratoplasty (DSAEK) with 51-99 μm and <50 μm lenticules.</p><p><strong>Methods: </strong>Retrospective, multi-center case series of 480 eyes undergoing DSAEK with precut tissue from a single eye bank between 2019 and 2022 performed by five surgeons at five centers. Eyes were divided into 51-99 μm or <50 μm groups according to post-cut graft thickness. The main outcome measure was inter-group comparison according to best-corrected distance visual acuity (BCDVA); additional outcome measures were primary graft failure (PGF), rebubbling, and cystoid macular edema (CME) rates in context of specific preoperative risk factors, intraoperative characteristics, and vision-limiting vs. non-vision-limiting morbidities.</p><p><strong>Results: </strong>BCDVA at one year postoperatively was 0.5 ± 0.5 and 0.4 ± 0.4 logMAR for the 51-99 μm and <50 μm groups, respectively (<i>p</i> = 0.692). Regraft rate was 3.21% for 51-99 μm and 1.89% for <50 μm grafts (<i>p</i> = 0.4854). Rebubbling rate was 8.56% for the 51-99 μm group and 13.21% for the <50 μm group (<i>p</i> = 0.151). Analysis of rebubbled eyes showed no difference in rate of sulfur hexafluoride tamponade (<i>p</i> = 0.201), lenticule insertion method (<i>p</i> = 0.293), and glaucoma surgery history (<i>p</i> = 0.996). Higher rebubbling rates occurred in eyes with previous scleral-fixated intraocular lenses (<i>p</i> < 0.001). The effect of potential preoperative and intraoperative risk factors (e.g. insertion method (<i>p</i> = 0.979) and concurrent cataract surgery (<i>p</i> = 0.701)) on rebubbling was not significantly different between the two groups. CME rate was 4.01% for 51-99 μm and 7.54% for <50 μm groups (<i>p</i> = 0.131).</p><p><strong>Conclusions: </strong>51-99 μm and <50 μm DSAEK grafts offer similar visual outcomes. Both lenticule thicknesses have similar regrafting, rebubbling, and CME rates.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"481-489"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to the Letter to the Editor: Expression of PAX6 and Keratocyte-Characteristic Markers in Human Limbal Stromal Cells of Congenital Aniridia and Healthy Subjects, <i>In Vitro</i>.","authors":"Zhen Li, Tanja Stachon, Julia Zimmermann, Simon Trusen, Fabian N Fries, Maximilian Berger, Shweta Suiwal, Ning Chai, Berthold Seitz, Lei Shi, Maryam Amini, Nóra Szentmáry","doi":"10.1080/02713683.2025.2497335","DOIUrl":"https://doi.org/10.1080/02713683.2025.2497335","url":null,"abstract":"","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-2"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Driven Identification of Hematological and Immunological Biomarkers for Predicting Proliferative Diabetic Retinopathy Progression.","authors":"Sibo Zhao","doi":"10.1080/02713683.2025.2498035","DOIUrl":"https://doi.org/10.1080/02713683.2025.2498035","url":null,"abstract":"<p><strong>Purpose: </strong>Proliferative Diabetic Retinopathy (PDR) is a severe complication of diabetes characterized by neovascularization and retinal detachment, leading to significant vision loss. This study investigates the predictive power of hematological and immunological markers in PDR progression.</p><p><strong>Methods: </strong>Data from 126 patients were analyzed using advanced machine learning techniques, including LASSO regression, elastic net modeling, and backward stepwise regression.</p><p><strong>Results: </strong>The findings identified age, gender, IL-1, and lymphocyte count (LYM) as significant predictors of PDR, with a high AUC value of 0.839 from the ROC curve analysis. These markers, particularly cytokines in the aqueous humor and peripheral blood, offer a convenient and rapid method for early detection and risk assessment of PDR.</p><p><strong>Conclusions: </strong>Despite the limitations of being a cross-sectional study with a relatively small sample size, the results highlight the clinical significance of these biomarkers and underscore the need for further validation in larger, more diverse populations. This study contributes to the development of targeted interventions and improved management strategies for diabetic retinopathy, emphasizing the importance of immunological health in disease progression.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Efficacy of an Assistive Device for Blind People: A Prospective, Non-Randomized, Single Arm, and Open Label Clinical Trial.","authors":"Yiming Zhao, Shuo Yang, Yong Tao, Hao Kang","doi":"10.1080/02713683.2025.2495212","DOIUrl":"https://doi.org/10.1080/02713683.2025.2495212","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the efficacy of an assistive device (BrainPort^® Vision Pro) for blind people in object recognition and orientation-and-mobility.</p><p><strong>Methods: </strong>This prospective, single arm, and open label clinical trial enrolled 24 participants who had profound visual impairment (visual acuity < 1/60 and/or visual field constriction <5°). After a three-hour training of BrainPort Vision Pro device instruction, participants were instructed to perform functional tasks including an assistive walking program and object recognition project. Five chances were given for each task, and success was defined as correctly completing at least three times. Success rates were used to evaluate the effectiveness of the device.</p><p><strong>Results: </strong>The success rates of participants wearing the device were significantly higher for both orientation-and-mobility task and objects/place-setting recognition tasks, compared to those without the aid. Device-related adverse events did not occur throughout the study.</p><p><strong>Conclusion: </strong>This is the first attempt to evaluate the efficacy of the BrainPort Vision Pro for blind people in China. It is shown as a viable assistive device for blind individuals to directly interact with their environments through a real perception from tongue stimulation, without remarkable side effects in the current study. With the advancement and popularization, more blind people will have access to and benefit from the BrainPort Vision Pro in the future.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143984194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}