A Proteomic Study of Different Types of Diabetic Macular Edema.

IF 2 4区 医学 Q3 OPHTHALMOLOGY
Current Eye Research Pub Date : 2025-10-01 Epub Date: 2025-07-22 DOI:10.1080/02713683.2025.2516806
Shuang Wu, Xin Zhou, Libo Wang, Dongyi Yu, Zhiyue Wang, Xuguo Xu
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引用次数: 0

Abstract

Purpose: This study investigates distinct protein markers associated with different morphologic features of diabetic macular edema, focusing on diffuse retinal thickening, cystoid macular edema, and cystoid macular degeneration.

Methods: A total of 48 eyes of 33 patients with diabetic macular edema were included in the study and categorized into the aforementioned groups. Preoperative aqueous humor samples were collected from nine diabetic macular edema patients and subjected to tandem mass spectrometry labeling using tandem mass tags quantitative proteomics analysis to compare protein profiles in the aqueous humor among the different morphologic features of diabetic macular edema.

Results: The analysis revealed that the efficacy of anti-vascular endothelial growth factor treatment was significantly better in the diffuse retinal thickening and cystoid macular edema groups compared to the cystoid macular degeneration group (P < 0.05). Differential expression analysis identified 31 upregulated and 43 downregulated proteins in the diffuse retinal thickening group compared to the cystoid macular degeneration group, with upregulated proteins associated with vasoconstriction, apoptotic processes, complement system, and peptidase inhibitors, and downregulated proteins associated with protein kinase and positive regulation of cell cycle regulation. Similarly, the cystoid macular edema group showed 31 upregulated and 32 downregulated proteins compared to the cystoid macular degeneration group, with upregulated proteins associated with the Wnt signaling pathway and extracellular matrix (ECM)-receptor interaction, and downregulated proteins associated with high-density lipoprotein (HDL), cholesterol positive regulation. The screening criteria for differentially expressed proteins were fold change >1.2 or <0.83 and P < 0.05.

Conclusion: Associations were found between cystoid macular degeneration formation and factors like the complement system, apoptotic pathway, and lipid metabolizing factors, providing insights for further understanding diabetic macular edema pathogenesis.

不同类型糖尿病黄斑水肿的蛋白质组学研究。
目的:本研究探讨与糖尿病黄斑水肿不同形态学特征相关的不同蛋白标志物,重点关注视网膜弥漫性增厚、囊样黄斑水肿和囊样黄斑变性。方法:将33例糖尿病性黄斑水肿患者48只眼分为上述两组。收集9例糖尿病黄斑水肿患者术前房水样本,采用串联质谱标记定量蛋白质组学分析,比较房水中不同形态特征的糖尿病黄斑水肿的蛋白质谱。结果:分析显示,视网膜弥漫性增厚组和囊样黄斑水肿组抗血管内皮生长因子治疗的疗效明显优于囊样黄斑变性组(P < 1.2)或P < 1.2)。发现囊样黄斑变性的形成与补体系统、凋亡通路、脂质代谢因子等因素有关,为进一步了解糖尿病黄斑水肿的发病机制提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Eye Research
Current Eye Research 医学-眼科学
CiteScore
4.60
自引率
0.00%
发文量
163
审稿时长
12 months
期刊介绍: The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.
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