防腐剂能否阻止2023年铜绿假单胞菌人工泪液相关眼部感染的爆发?

IF 2 4区 医学 Q3 OPHTHALMOLOGY
Eric G Romanowski, Michael E Zegans, Robert M Q Shanks
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引用次数: 0

摘要

目的:2023年爆发的广泛耐药(XDR)铜绿假单胞菌(PA)眼部感染与使用在线购买的非保存的多用途人工泪液有关。缺乏防腐剂被认为是爆发的主要因素。这使我们开展了当前的研究,目的是确定普通的眼科防腐剂是否可以消除XDRPA和其他PA角膜炎分离株的爆发。材料和方法:采用muler - hinton broth (MHB)和phosphate buffered saine (PBS)对包括XDRPA暴发菌株在内的3株PA菌株进行了时效研究。评估的防腐剂为苯扎氯铵(BAK) 0.04%、0.01%和0.004%、聚季铵盐-1 (Polyquad) (PQ) 0.001%、过硼酸钠(SP) 0.1%和EDTA 0.1%。此外,使用保存的和非保存的市售人工泪液进行时效测定。结果:BAK分别为0.04%和0.01%,PQ在4 h后将两种培养基中的PA分离株全部清除。0.004%的BAK和SP在PBS中1 h杀灭了所有的PA菌株,但在24 h内对MHB没有产生99.9%的杀菌作用。EDTA在两种介质中均不产生杀菌作用。含有0.25%山梨酸和0.1%乙酸二钠的人工泪液,PQ、SP和PURITE在24 h内消除了PA菌株。结论:几种常用防腐剂可有效杀灭包括爆发菌株CDC1270在内的PA菌株。与PBS相比,富培养基(MHB)中的防腐效果被延迟或抑制。这些结果表明,可以通过在人工泪液中使用一种常见的防腐剂来预防2023年的爆发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Could a Preservative Have Prevented the Extremely-Drug Resistant Pseudomonas aeruginosa Artificial Tears Associated Eye Infection Outbreak of 2023?

Purpose: The 2023 outbreak of extensively drug resistant (XDR) Pseudomonas aeruginosa (PA) eye infections was linked to the use of non-preserved, multi-use artificial tears purchased online. The lack of preservatives has been attributed to be the predominant factor in the outbreak. This led us to the current study for which the goal was to determine whether common ophthalmic preservatives could eliminate the outbreak XDRPA and other PA keratitis isolates.

Materials and methods: Time-kill studies were performed in Mueller-Hinton broth (MHB) and phosphate buffered saine (PBS) on 3 PA strains including the XDRPA outbreak strain. Preservatives assessed were benzalkonium chloride (BAK) 0.04%, 0.01%, and 0.004%, Polyquaterium-1 (Polyquad) (PQ) 0.001%, Sodium Perborate (SP) 0.1%, and EDTA 0.1%. In addition, time-kill assays were performed using preserved and a non-preserved commercially-available artificial tears.

Results: BAK 0.04% and 0.01%, and PQ eliminated all PA isolates in both media after 4 h. BAK 0.004% and SP eliminated all PA strains in PBS at 1 h but did not produce bactericidal 99.9% decreases in MHB over 24 h. EDTA did not produce a bactericidal effect in either media. Artificial tears containing sorbic acid 0.25% and edetate disodium 0.1%, PQ, SP, and PURITE eliminated the PA strains within 24 h.

Conclusions: Several common preservatives were effective in eliminating PA strains including the outbreak strain CDC1270. Preservative efficacy was delayed or inhibited in rich media (MHB) compared to PBS. These results suggest that the 2023 outbreak could have been prevented by using a common preservative in the artificial tears.

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来源期刊
Current Eye Research
Current Eye Research 医学-眼科学
CiteScore
4.60
自引率
0.00%
发文量
163
审稿时长
12 months
期刊介绍: The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.
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