延长晶状体近视对小鼠视网膜结构和功能的影响。

IF 2 4区 医学 Q3 OPHTHALMOLOGY
Yajing Yang, Yohei Tomita, Deokho Lee, Shin-Ichi Ikeda, Xiaoyan Jiang, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
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引用次数: 0

摘要

目的:研究小鼠长晶状体性近视模型中视网膜结构和功能的时间依赖性改变。方法:从出生后第3周开始,采用双眼- 30d晶状体植入雄性C57BL/6J小鼠,诱导近视。小鼠分为长期诱导组(3-9周龄)和短期诱导组(3-6周龄)。另一组装有0 - D透镜作为对照。在第3、6和9周使用红外折射仪和光谱域光学相干断层扫描测量眼部参数,包括屈光度、轴向长度、脉络膜厚度和视网膜厚度。长期组和短期组分别在第9周和第6周用暗适应视网膜电图评估视网膜功能。统计分析包括双向重复测量方差分析,然后用Bonferroni事后检验进行组间和时间点的比较,以及双尾非配对t检验或单因素方差分析,用Fisher’s LSD事后检验进行组间各时间点的比较。结果:近视诱导6周后,长期近视组屈光、眼轴长度延长、脉络膜厚度变薄明显降低。视网膜内厚度明显减少,视网膜电图显示振荡电位的振幅衰减和隐式时间延迟。结论:小鼠长时间晶状体性近视可引起视网膜结构和功能的改变,尤其是内层的改变。该模型强调了长期近视条件下早期视网膜监测的重要性,并为研究视网膜易损性和制定长期保护策略提供了平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of Extended Lens-Induced Myopia on Retinal Structure and Function in Mice.

Purpose: To investigate time-dependent structural and functional retinal alterations in a mouse model of prolonged lens-induced myopia.

Methods: Myopia was induced in male C57BL/6J mice by fitting -30 D lenses on both eyes starting at postnatal week 3. Mice were assigned to either a long-term induction group (3-9 weeks of age) or a short-term induction group (3-6 weeks of age). Separate groups fitted with 0 D lenses served as controls. Ocular parameters, including refraction, axial length, choroidal thickness, and retinal thickness were measured at weeks 3, 6, and 9 using an infrared photorefractor and spectral-domain optical coherence tomography. Retinal function was assessed by dark-adapted electroretinography at week 9 for the long-term group and at week 6 for the short-term group. Statistical analyses included two-way repeated-measures ANOVA followed by Bonferroni's post hoc test for comparisons across groups and time points, and two-tailed unpaired t-test or one-way ANOVA with Fisher's LSD post hoc test for group comparisons at individual time points.

Results: After 6 weeks of myopia induction, the long-term myopia group exhibited significant reduction in refraction, axial length elongation, and choroidal thickness thinning. Inner retinal thickness was markedly reduced, and electroretinography revealed attenuated amplitudes and delayed implicit times of the oscillatory potentials.

Conclusion: Prolonged lens-induced myopia in mice induces time-dependent structural and functional retinal alterations, particularly in the inner layers. This model underscores the significance of early retinal monitoring under long-term myopic conditions and serves as a platform for investigating retinal vulnerability and developing preservation strategies over time.

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来源期刊
Current Eye Research
Current Eye Research 医学-眼科学
CiteScore
4.60
自引率
0.00%
发文量
163
审稿时长
12 months
期刊介绍: The principal aim of Current Eye Research is to provide rapid publication of full papers, short communications and mini-reviews, all high quality. Current Eye Research publishes articles encompassing all the areas of eye research. Subject areas include the following: clinical research, anatomy, physiology, biophysics, biochemistry, pharmacology, developmental biology, microbiology and immunology.
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