Current Eye Research最新文献

{"title":"Bioinformatics for the Identification of STING-Related Genes in Diabetic Retinopathy.","authors":"Yu Wang, Siyan Liu, Qi Zhou, Yalin Feng, Qin Xu, Linbi Luo, Hongbin Lv","doi":"10.1080/02713683.2024.2430223","DOIUrl":"https://doi.org/10.1080/02713683.2024.2430223","url":null,"abstract":"<p><strong>Purpose: </strong>Diabetic retinopathy (DR) is the most common complication of diabetes mellitus. Stimulator of interferon genes (STING) plays an important regulatory role in the transcription of several genes. This study aimed to mine and identify hub genes relevant to STING in DR.</p><p><strong>Methods: </strong>The STING-related genes (STING-RGs) were extracted from MSigDB database. Differentially expressed STING-RGs (DE-STING-RGs) were filtered by overlapping differentially expressed genes (DEGs) between DR and NC specimens and STING-RGs. A PPI network was established to mine hub genes. The ability of the hub genes to differentiate between DR and NC specimens was evaluated. Additionally, a ceRNA network was established to investigate the regulatory mechanisms of hub genes. Subsequently, the discrepancies in immune infiltration between DR and NC specimens were further explored. Additionally, we performed drug predictions. Finally, RT-qPCR of peripheral blood samples was used to validate the bioinformatics results.</p><p><strong>Results: </strong>A grand total of four genes (IKBKG, STAT6, NFKBIA, and FCGR2A) related to STING were identified for DR. The AUC values of all four hub genes were greater than 0.7, which indicated that the diagnostic value was acceptable. The ceRNA network contained four hub genes, 170 miRNAs, and 135 lncRNAs. In addition, immunoinfiltration analysis demonstrated that the abundance of activated B cells was notably different between the DR and NC specimens. Moreover, 32 drugs were included in the drug-gene network, with twelve drugs targeting STAT6, nine drugs targeting NFKBIA, four drugs targeted IKBKG, and seven drugs targeted FCGR2A. The expression of the four hub genes in blood samples determined by RT-qPCR was consistent with our analysis.</p><p><strong>Conclusion: </strong>In conclusion, four hub genes (IKBKG, STAT6, NFKBIA, and FCGR2A) related to STING with a diagnostic value for DR were identified by bioinformatics analysis, which might provide new insights into the evaluation and treatment of DR.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-14"},"PeriodicalIF":1.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142863416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Lens Properties on Visual Acuity, Aniridia Associated Keratopathy and Secondary Glaucoma in Congenital Aniridia Subjects.","authors":"Annamária Náray, Fabian N Fries, Cristian Munteanu, Mária Csidey, Tanja Stachon, Neil Lagali, Achim Langenbucher, Barbara Käsmann-Kellner, Berthold Seitz, Nóra Szentmáry","doi":"10.1080/02713683.2024.2438687","DOIUrl":"https://doi.org/10.1080/02713683.2024.2438687","url":null,"abstract":"<p><strong>Purpose: </strong>The potential risks and benefits of cataract surgery, in context of congenital aniridia (CA), are not widely understood, yet. Our purpose was to investigate the effect of lens properties on visual acuity (VA), aniridia-associated keratopathy (AAK) stage and presence of glaucoma at the Homburg Aniridia Center.</p><p><strong>Methods: </strong>CA subjects, examined at the Department of Ophthalmology of Saarland University between June 2003 and January 2022, were included. VA, slit-lamp examination, AAK grade, and glaucoma evaluation data were extracted from the medical records, from the first visit to the center. Eyes were categorized as clear lens, cataract, pseudophakic, aphakic, or subluxated lens. Patients were grouped by age (0-10, 10-20, 20-40, 40+ years).</p><p><strong>Results: </strong>In 553 eyes of 286 CA subjects (age 19.9 ± 19.9 (0-83) years, 46.1% males), analysis revealed significant differences in VA and mean IOP (ANOVA <i>p</i> < 0.0001; <i>p</i> = 0.001, respectively) with lens status. Lens status was strongly associated with AAK Grade and glaucoma presence (<i>p</i> < 0.0001 for both). In age subgroups, AAK Stage was strongly associated with lens status in the 0-10 years (<i>p</i> < 0.001), 10-20 years (<i>p</i> < 0.001), and 40+ years (<i>p</i> = 0.02) groups and lens status was strongly associated with glaucoma presence in the 0-10 years (<i>p</i> = 0.003) and 20-40 years (<i>p</i> = 0.002) groups. AAK Stage was the most advanced in pseudophakic and aphakic eyes and presence of glaucoma was more pronounced in pseudophakic, aphakic and subluxated lens eyes.</p><p><strong>Conclusions: </strong>In a large population of CA, previous cataract surgery was associated with higher AAK Grade and presence of secondary glaucoma both in postoperatively pseudophakic and aphakic eyes. Our data indicate that caution is warranted with cataract surgery in congenital aniridia.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SMP30 Alleviates Oxidative Stress and Regulates Ca²⁺-ATPase Activity in UVR-B-Induced Cataracts in Rats.","authors":"Tian Lan, Yongshun Liang, Qingqiao Gan, Hao Liang","doi":"10.1080/02713683.2024.2441253","DOIUrl":"https://doi.org/10.1080/02713683.2024.2441253","url":null,"abstract":"<p><strong>Purpose: </strong>Oxidative stress, ultraviolet radiation, and calcium imbalance are key components in the onset and advancement of cataract, which continue to be the leading cause of blindness globally. An important newly discovered aging maker, Senescence marker protein 30 (SMP30) regulates calcium and participates in mitigating oxidative stress damage. Here, we examined the beneficial role of SMP30 in protecting against ultraviolet radiation type B (UVR-B)-induced cataract in rats.</p><p><strong>Methods: </strong>Wistar rats (2 months) were arbitrarily assigned into 4 groups of 10 rats. These groups included the Control group, UVR-B group, adeno-associated virus 2 vectors negative control (AAV2-NC) group, and adeno-associated virus 2-mediated overexpression of SMP30 (AAV2-SMP30) group. The control group received Phosphate-buffered saline (PBS) <i>via</i> injection, while the AAV2-NC group and AAV-SMP30 group were separately injected with AAV2-NC and AAV2-SMP30 vectors. In addition to the control group, the remaining three experimental groups were subjected to ultraviolet light exposure 4 weeks post-injection. The lens opacity was examined by stereoscopic microscope, and the lenses were separated to measure oxidative damage parameters particularly superoxide dismutase (SOD), glutathione peroxidase (GPX), and Ca²⁺-ATPase activity. The localization and expression of SMP30 and Ca²⁺-ATPase in the lenses were determined using immunohistochemistry and RT-qPCR.</p><p><strong>Results: </strong>After UVR-B irradiation, the AAV2-SMP30 group exhibited a substantial decrease in lens opacity compared to the UVR-B group. The results revealed a notable downregulation of SMP30 expression and the activities of SOD, GPX, and Ca²⁺-ATPase of rat lens following exposure to UVR-B radiation. However, SMP30 overexpression partially reversed these effects. <i>In vivo</i> experiments demonstrated SMP30 overexpression attenuated the UVR-B-induced decline in SOD, GPX, and Ca²⁺-ATPase activities.</p><p><strong>Conclusion: </strong>This study demonstrates that SMP30 has the potential to reduce lens opacity caused by UVR-B by increasing antioxidant stress and regulating Ca²⁺-ATPase activity. SMP30 might be a cutting-edge target for the treatment of cataracts.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of miR-21/NLRP3 on Blue Light-Induced Retinal Neurodegeneration in Mice.","authors":"Yi Zhang, Xingzhao Xu","doi":"10.1080/02713683.2024.2419684","DOIUrl":"https://doi.org/10.1080/02713683.2024.2419684","url":null,"abstract":"<p><strong>Purpose: </strong>Age-related macular degeneration (AMD) is a chronic retinal disease that can lead to blindness. While the NLR family pyrin domain containing 3 (NLRP3) inflammasome is implicated in AMD, the specific roles of miR-21 and NLRP3 in AMD-related inflammation remain unclear. Therefore, this study aimed to investigate the roles of miR-21 and NLRP3 in blue light-induced neurodegeneration in the mouse retina.</p><p><strong>Methods: </strong>A mouse model of retinal light damage was established through three months of blue light exposure (BLE). The experimental groups comprised the Control (Ctrl), BLE, BLE + miR-nc, and BLE + miR-21 inhibitor groups. The microRNAs were administered <i>via</i> intravitreal injections once per week. After successful modeling, changes in visual function and retinal morphology were investigated by using electroretinography and hematoxylin and eosin staining, respectively. Photoreceptor apoptosis was assessed using the TdT-mediated dUTP nick-end labeling assay. Immunofluorescence was used to detect and locate microglia and NLRP3 expression in the mouse retina. The expression of miR-21, NLRP3, and downstream factors in the retinas of each group was measured using qRT-PCR and western blotting.</p><p><strong>Results: </strong>In the BLE and BLE + miR-nc groups, there was a decrease in visual function and retinal thickness, an increase in retinal ganglion cell injury and photoreceptor cell apoptosis, and elevated microglia activity in the retina, as evidenced by their migration to the outer retinal layer. In addition, the expression of miR-21, NLRP3, and downstream factors was increased in the BLE and BLE + miR-nc groups compared to that in the control group. However, intravitreal injection of the miR-21 inhibitor reduced miR-21 expression in the retina and significantly inhibited the activation of the NLRP3 inflammasome, effectively alleviating retinal photodamage caused by BLE.</p><p><strong>Conclusions: </strong>This study indicates that miR-21 may mitigate blue-light-induced retinal neurodegeneration by reducing the activation of the NLRP3 inflammasome in the mouse retina.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of Visual Acuity After Cataract Surgery by Deep Learning Methods Using Clinical Information and Color Fundus Photography.","authors":"Che-Ning Yang, Yi-Ting Hsieh, Hsu-Hang Yeh, Hsiao-Sang Chu, Jo-Hsuan Wu, Wei-Li Chen","doi":"10.1080/02713683.2024.2430212","DOIUrl":"https://doi.org/10.1080/02713683.2024.2430212","url":null,"abstract":"<p><strong>Purpose: </strong>To examine the performance of deep-learning models that predicts the visual acuity after cataract surgery using preoperative clinical information and color fundus photography (CFP).</p><p><strong>Methods: </strong>We retrospectively collected the age, sex, and logMAR preoperative best corrected visual acuity (preoperative-BCVA) and CFP from patients who underwent cataract surgeries from 2020 to 2021 at National Taiwan University Hospital. Feature extraction of CFP was performed using a pre-existing image classification model, Xception. The CFP-extracted features and pre-operative clinical information were then fed to a downstream neural network for final prediction. We assessed the model performance by calculating the mean absolute error (MAE) between the predicted and the true logMAR of postoperative BCVA. A nested 10-fold cross-validation was performed for model validation.</p><p><strong>Results: </strong>A total of 673 fundus images from 446 patients were collected. The mean preoperative BCVA and postoperative BCVA was 0.60 ± 0.39 and 0.14 ± 0.18, respectively. The model using age and sex as predictors achieved an MAE of 0.121 ± 0.016 in postoperative BCVA prediction. The inclusion of CFP as additional predictor in the model (predictors: age, sex and CFP) did not further improve the predictive performance (MAE = 0.120 ± 0.023, <i>p</i> = 0.375), while adding the preoperative BCVA as an additional predictor resulted in a 4.13% improvement (predictors: age, sex and preoperative BCVA, MAE = 0.116 ± 0.016, <i>p</i> = 0.013).</p><p><strong>Conclusions: </strong>Our multimodal models including both CFP and clinical information achieved excellent accuracy in predicting BCVA after cataract surgery, while the learning models input with only clinical information performed similarly. Future studies are needed to clarify the effects of multimodal input on this task.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Chronic Heavy Metal Exposure by Analysis of Human Cataract Specimens and the Relationship to Metabolic Profiles.","authors":"Matthew Li, Michelle Gende, Alyssa Tovar, Martina Schmeling, Susanne Tidow-Kebritchi, Bruce I Gaynes","doi":"10.1080/02713683.2024.2421932","DOIUrl":"https://doi.org/10.1080/02713683.2024.2421932","url":null,"abstract":"<p><strong>Purpose: </strong>With age, the mammalian lens forms successive layers of crystallin protein fibers which infoliate with lens growth and development. As heavy metals generally bind to tissue protein, heavy metals are posited to sequester within the lens with age. Therefore, this study aims to compare heavy metals in human crystalline lens of older adults to known physiologic blood and urine levels and assess the association between concentrations in the lens and metabolic biomarkers.</p><p><strong>Methods: </strong>Consecutive lens specimens obtained during cataract surgery by phacoemulsification were subjected to atomic spectrometry for heavy metal content. A one-sample <i>t</i>-test compared heavy metals in lens to known physiologic blood and urine concentrations. Linear regression models assessed the association between heavy metals and biomarkers of metabolic function. Linear discriminant analysis assessed the classification of gender and smoking status based on multiple and individual heavy metals.</p><p><strong>Results: </strong>All heavy metal levels were elevated in lens specimens compared to blood and urine with the exception of iron (<i>p</i> < 0.0001). Lens titanium and copper were positively associated with blood-urea nitrogen (Titanium: <math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.14, <i>p</i> = 0.04, Copper: <math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.12, <i>p</i> = 0.03. Lens copper was positively associated with creatinine (<math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.10; <i>p</i> = 0.02), but negatively associated with glomerular filtration rate (<math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 0.89; <i>p</i> = 0.02). Lens chromium and lead were positively associated with albumin (Chromium: <math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.03, <i>p</i> = 0.03; Lead: <math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.02, <i>p</i> = 0.04). Lens nickel was positively associated with bilirubin (<math><mrow><mrow><mrow><mover><mrow><mi>β</mi></mrow><mo>̂</mo></mover></mrow></mrow></mrow></math> = 1.14; <i>p</i> = 0.03). Classification based on multiple or individual heavy metals for gender and smoking status was not statistically significant.</p><p><strong>Conclusions: </strong>Our results suggest the human crystalline lens accumulates heavy metals with age and demonstrate the correlation between abnormality of metabolic function and heavy metal deposition in older adult lens.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OCTA and Microperimetry Changes Preceding the Appearance of Diabetic Retinopathy in Patients with Type 1 Diabetes.","authors":"Daniela Rego-Lorca, Manel Mateu-Salat, Ana Chico, Alberto Molina-Montero, Jesús Díaz-Cascajosa, José Ignacio Vela-Segarra","doi":"10.1080/02713683.2024.2435357","DOIUrl":"https://doi.org/10.1080/02713683.2024.2435357","url":null,"abstract":"<p><strong>Purpose: </strong>to evaluate changes in retinal microvasculature and sensitivity (RS) preceding the appearance of diabetic retinopathy (DR) among patients with type 1 diabetes (T1D).</p><p><strong>Methods: </strong>in this observational cross-sectional cohort study, vascular parameters measured by OCTA and RS evaluated by microperimetry were assessed in patients with T1D without DR (no-DR), T1D with mild DR (m-DR), and healthy controls.</p><p><strong>Results: </strong>Sixty-two eyes of 31 patients with T1D and 40 eyes of 20 healthy patients were included. OCTA examinations did not yield any significant differences in terms of perfusion density (PD), vascular density (VD), foveal avascular zone (FAZ) area, FAZ perimeter or FAZ circularity between patients with diabetes (no-DR <i>vs.</i> m-DR). However, comparisons between healthy controls and patients with diabetes (both no-DR and m-DR groups) revealed statistically significant differences in PD, VD, and FAZ area. Similarly, no significant differences were observed between no-DR and m-DR groups regarding RS, gaze fixation stability (GFS), or macular integrity (MI). Nevertheless, mean RS and MI were significantly impaired in patients with T1D, both in no-DR and m-DR groups, compared to healthy controls. A statistically significant positive correlation was observed between RS and PD and between FAZ area and RS.</p><p><strong>Conclusion: </strong>although no differences were found between patients with diabetes without DR and those with mild DR, these patients already demonstrated some degree of retinal impairment, both structural and functional, when compared to healthy controls. Our data support the hypothesis that neurodegeneration occurs together with microvascular damage at early stages of diabetes.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-5"},"PeriodicalIF":1.7,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting CCL5 Attenuates Fibrosis via Activation of PI3k/Akt Signaling Axis After Glaucoma Filtration Surgery.","authors":"Baixue Zhu, Ran Wei, Xinying Li, Qingyun Bi","doi":"10.1080/02713683.2024.2432399","DOIUrl":"https://doi.org/10.1080/02713683.2024.2432399","url":null,"abstract":"<p><strong>Purpose: </strong>Glaucoma filtration surgery (GFS) stands as a paramount clinical intervention for glaucoma. Nonetheless, the prevalent cause of GFS failure is filtration bleb scarring, and the role of inflammation and immune response in contributing to fibrosis remains elusive.</p><p><strong>Methods: </strong>The study employed 30 female Sprague-Dawley rats (8 weeks old, 200-250 g) to assess the anti-scarring impact of the Chemokine (C-C motif) receptor 5 (CCR5)-Chemokine (C-C motif) ligand 5 (CCL5) antibody after GFS. Additionally, anti-fibrotic effects on HConFs were examined, creating an intra-operative inflammatory response using damaged-HConFs supernatant medium (DHSM). <i>In vitro</i> and <i>in vivo</i> validation aimed to elucidate the potential anti-fibrotic molecular mechanism of the CCR5-CCL5 antibody.</p><p><strong>Results: </strong>The CCR5-CCL5 antibody effectively prolonged filtration bleb duration and enhanced the functionality of the filtered bleb. Improved postoperative intraocular pressure values (IOP) and morphological images were observed in the CCR5-CCL5 antibody-treated group. Histochemical staining and cellular experiments confirmed the antifibrotic function of the CCR5-CCL5 antibody. Notably, M2-type macrophage polarization was reduced in the CCR5-CCL5 antibody-treated model. CCL5-induced fibrosis in HConFs was mediated through the PI3K/Akt signaling pathway. Consistently, inhibition of PI3K/Akt significantly attenuated the profibrotic effects of CCR5-CCL5. Mechanistically, the CCL5 antibody exerts its antifibrotic effect by targeting CCR5 on HConFs, leading to the inhibition of the PI3K/Akt mechanism.</p><p><strong>Conclusions: </strong>This study unveils that CCR5-CCL5 promotes fibrosis in GFS through inflammatory stimulation of HConFs and enhanced activation of the PI3K/Akt signaling pathway. The findings suggest that intraoperative CCR5-CCL5 antibody treatment could serve as a cost-effective therapeutic agent or a useful adjuvant in preventing ocular bleb scar formation.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Laurus Nobilis</i> Eye Drop on Selenite-Induced Cataract Formation and Oxidative Stress-Related Parameters in Rabbits: An Experimental Study.","authors":"Marisa Palazzo, Marina Concilio, Luigi Ambrosone, Michele Rinaldi, Fausto Tranfa, Ciro Costagliola","doi":"10.1080/02713683.2024.2380440","DOIUrl":"10.1080/02713683.2024.2380440","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the protective role of <i>Laurus Nobilis</i> eye drops on selenite-induced cataracts in suckling rabbits.</p><p><strong>Methods: </strong>Fifteen male albino suckling rabbits with no signs of ocular inflammation were randomly assigned to three groups: controls (Group A), sodium-selenite group (Group B) and sodium-selenite plus <i>Laurus Nobilis</i> group (Group C). By selenite treatment, cataract formation was experimentally induced and then graded. The grade of oxidative stress was defined in the lens, measuring the concentration of malondialdehyde, alpha-tocopherol, oxidized glutathione, ascorbic acid and hydrogen peroxide, and in blood samples as levels of alpha-tocopherol and malondialdehyde.</p><p><strong>Results: </strong>Mean lens concentrations of GSSG, H2O2, and MDA levels in group B were significantly higher than in both group C and control. Ascorbic acid and alpha-tocopherol concentrations were lower in group B than in both group C and A. As plasma oxidative status markers, the level of MDA was higher in group B respected group C and A. The mean alpha-tocopherol levels in group B were significantly lower than in both group A and group C.</p><p><strong>Conclusions: </strong>In animals treated with <i>Laurus Nobilis</i>-based eye drops, inflammation was inhibited, and lipid peroxidation was significantly reduced. <i>Laurus nobilis</i> leaves extract represents a good source of antioxidant components that may contrast sodium selenite-induced cataractogenesis in suckling rabbits.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1247-1252"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ripasudil Hydrochloride on Epithelial Repair in a Mouse Cornea.","authors":"Yukihisa Takada, Shingo Yasuda, Takayoshi Sumioka, Yuka Okada, Tadahiko Tamura, Osamu Yamanaka, Shizuya Saika","doi":"10.1080/02713683.2024.2378018","DOIUrl":"10.1080/02713683.2024.2378018","url":null,"abstract":"<p><strong>Purpose: </strong>Effect of topical administration of a Rho kinase inhibitor, ripasudil, on epithelial wound healing in a mouse cornea was investigated. Effects of treatment of cultured human corneal epithelial cell (HCEC) line and organ-cultured corneal epithelium with ripasudil on expression of p-ERK was also examined.</p><p><strong>Methods: </strong>Epithelial defects with a diameter of 2.0 mm were prepared in the central corneas of C57BL/6 mice with or without 1-week travoprost pre-treatment, to which ripasudil or PBS as a control was instilled every 6 h immediately after preparation. The mice eyes were cultured with or without travoprost for 24-hrs. The expression levels of p-ERK in epithelium of mice eyes were compared by immunostaining after further 24-hrs culture with or without ripasudil for 24-hrs. HCEC were cultured with or without ripasudil and processed for examination for proliferation activity and protein expression of p-ERK by either immunostaining or Western blotting. The cells were also treated with or without travoprost for 24-hrs, and were further cultured with or without ripasudil. Expression levels of p-ERK were examined by Western blotting.</p><p><strong>Results: </strong>Ripasudil treatment suppressed post-debridement epithelial healing in association with reduced proliferation activity in peripheral (limbal) epithelium in cornea with or without pre-treatment with travoprost. Ripasudil treatment accelerated p-ERK expression. Ripasudil supplementation upregulated proliferation with increased p-ERK in HCEC.</p><p><strong>Conclusion: </strong>Ripasudil treatment promotes wound healing of the mouse corneal epithelium by enhancing cell proliferation on peripheral (limbal) epithelium.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1215-1222"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}