Current Eye Research最新文献

{"title":"Research Best Practices for Eye Stroke Vision Rehabilitation: A Qualitative Study.","authors":"Kimberly Hreha, Nathan A Boucher, Brian Mac Grory, April Martina Burch, Emmanuel Acheampong, Heather E Whitson","doi":"10.1080/02713683.2025.2456788","DOIUrl":"10.1080/02713683.2025.2456788","url":null,"abstract":"<p><strong>Purpose: </strong>Central retinal artery occlusion, also known as an eye stroke, results in visual impairment and functional challenges. Our study objectives were to identify meaningful measures and factors that indicate or enable successful recovery after eye stroke and to determine optimal processes to support research, including exploring barriers and facilitators to successful research participation.</p><p><strong>Methods: </strong>We used qualitative methods including the 5Ts Framework (target population identification, team composition, time considerations, tips to accommodate older adults, tools for inclusive enrollment of older adults) to provide a guide to the development of the semi-structured interviews and to help facilitate the research process such as the set-up of interviews. We enrolled three groups: individuals living with the sequelae of eye stroke, care partners, and health care providers. We conducted a descriptive content analysis with an inductive approach.</p><p><strong>Results: </strong>Twenty-five people participated. This group included 10 eye stroke survivors (median age, 62.5; range, 56-84 years; 20% were women), 4 care partners (median age, 57 years; range, 56-59 years; 100% were women), and 11 health care providers (median age, 33 years; 25-60 years; 54.5% were women). Four themes emerged from the data: (1) returning to full independence, (2) family support, (3) strategies for participation, and (4) lack of accessibility.</p><p><strong>Conclusions: </strong>The themes identified by participant groups indicated that strategies such as providing sufficient lighting and incorporating family support are key for research participation by individuals with vision impairment related to eye stroke. Furthermore, it is essential that researchers determine ways to address inequities, including lack of accessibility to research sites. This work has implications for how research teams develop research processes and implement research findings into clinical settings for eye stroke survivors.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-7"},"PeriodicalIF":1.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Performance of Topical Insulin in Persistent Corneal Epithelial Defects and Persistent Corneal Ulcers - A Case Series.","authors":"Amalia Mester, Maximilian Gabriel, Ingrid Boldin, Haleh Aminfar, Nora Woltsche, Astrid Heidinger, Andreas Guttmann, Paul Wintersteller, Jutta Horwath-Winter","doi":"10.1080/02713683.2025.2456784","DOIUrl":"https://doi.org/10.1080/02713683.2025.2456784","url":null,"abstract":"<p><strong>Purpose: </strong>To retrospectively describe the performance of topical insulin in persistent corneal epithelial defects (CED) and persistent corneal ulcers.</p><p><strong>Methods: </strong>We reviewed cases of patients treated for persistent CED and persistent corneal ulcers using topical insulin in a concentration of 25 IU per milliliter three times per day. The closure rate of CED and corneal ulcers was the main outcome measure.</p><p><strong>Results: </strong>Thirty-seven episodes of 29 patients treated with topical insulin were reviewed. There was a wide range of additionally used medication, underlying pathologies as well as ocular and systemic comorbidities in our cohort. On average, insulin drops were started after 36 days of conventional therapy (SD 59, range 0-193) and were used for 42 days (SD 38, range 3-130). Therapy success was achieved in 15 of 28 (53.5%) cases with CED and in 4 of 9 (44%) cases with corneal ulcers. While insulin generally showed a good safety profile, one patient reported intolerable discomfort related to the use of topical insulin.</p><p><strong>Conclusion: </strong>Topical insulin may be considered as a treatment option in complicated cases refractory to conventional treatment, but outcomes may be less favorable than previously reported.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-4"},"PeriodicalIF":1.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome's Implications in Age-Related Macular Degeneration.","authors":"Ali Torkashvand, Amir Hajrasouliha","doi":"10.1080/02713683.2025.2457105","DOIUrl":"https://doi.org/10.1080/02713683.2025.2457105","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to conduct a mini review of published literature concerning the role of exosomes in the field of ophthalmology, with a specific focus on Age-Related Macular Degeneration (AMD).</p><p><strong>Methods: </strong>In this study, a comprehensive search was conducted using PubMed and Google Scholar to identify relevant publications. Additionally, trials submitted to clinicaltrials.gov were reviewed to identify further relevant articles. The selected studies specifically focused on the ocular implications of exosomes in Age-Related Macular Degeneration.</p><p><strong>Results: </strong>Exosomes, small extracellular vesicles measuring less than 200 nm, play a crucial role in cell signaling and are involved in various physiological and pathological processes. Recent research has focused on utilizing exosomes for disease detection and treatment. Studies have investigated the ocular implications of exosomes, particularly in AMD. Exosomes found in aqueous fluid and blood have been examined as potential markers for AMD and as indicators of treatment response. Additionally, research in animal models has indicated the potential of exosomes in preventing AMD, as well as their promise for targeted and efficient drug delivery. This mini review primarily emphasizes the clinical aspects of publications related to AMD, rather than focusing solely on basic science.</p><p><strong>Conclusions: </strong>Exosomes have a great potential for understanding Age-related Macular Degeneration and effective and targeted treatment for retinal diseases.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Retinal Segmentation Metrics with Machine Learning for Predictions from Mouse SD-OCT Scans.","authors":"Maide Gözde İnam, Onur İnam, Xiangjun Yang, Qun Zeng, Gülgün Tezel","doi":"10.1080/02713683.2025.2456783","DOIUrl":"10.1080/02713683.2025.2456783","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to initially test whether machine learning approaches could categorically predict two simple biological features, mouse age and mouse species, using the retinal segmentation metrics.</p><p><strong>Methods: </strong>The retinal layer thickness data obtained from C57BL/6 and DBA/2J mice were processed for machine learning after segmenting mouse retinal SD-OCT scans. Twenty-two models were trained to predict the mouse groups. The best neural network model was optimized for better outcomes. Prediction accuracy, the area under the curve, sensitivity, specificity, precision, and F-1 score values were obtained.</p><p><strong>Results: </strong>The Wilcoxon Signed-Rank test provided significantly higher validation accuracy for neural networks than decision trees, discriminant analysis, support vector machines, and k-nearest neighbor classifiers (<i>p</i> = 0.005 for all). For C57BL/6-DBA/2J classification, a mean validation accuracy of 88.11 ± 3.92% (95% CI: 86.99-89.22) was achieved for the neural network when the optimized neural network had 92.31% final test accuracy with an area under the curve value of 0.9762, 94.44% sensitivity, 90.48% specificity, 89.47% precision, and 0.92 F-1 score. The optimized neural network model for age group differentiation had a final test accuracy of 82.05% with a 0.9064 area under the curve value, 77.27% sensitivity, 88.24% specificity, 89.47% precision, and 0.83 F-1 score.</p><p><strong>Conclusions: </strong>These findings validate that machine learning, using segmentation metrics instead of images, can effectively analyze retinal OCT scans in mice for categorical predictions in experimental models. Expanding this approach with additional features, including histopathological and functional correlations, is expected to improve the prediction power further, promising valuable applications to predict more complex outcomes in experimental and clinical studies.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-Analysis of the Association Between <i>VEGF</i>-2578C/A Polymorphism and Susceptibility to Type 2 Diabetic Retinopathy.","authors":"Dingyong Wu, Wanting Li","doi":"10.1080/02713683.2025.2451616","DOIUrl":"https://doi.org/10.1080/02713683.2025.2451616","url":null,"abstract":"<p><strong>Purpose: </strong>to investigate the association between vascular endothelial growth factor (<i>VEGF)</i>-2578C/A polymorphism and susceptibility to type 2 diabetic retinopathy (T2DR) by meta-analysis.</p><p><strong>Methods: </strong>According to the search strategy, Four databases were retrieved to identify the literature on the relationship between <i>VEGF</i> polymorphism and the risk of T2DR from inception to July 2024. Stata 15.0 was used for data processing.</p><p><strong>Results: </strong>Ten articles were involved in this review, covering 1390 cases and 1306 controls. The pooled results exhibited that the risk of T2DR was associated with <i>VEGF</i>-2578C/A polymorphism under the allele model (A/C: OR= 1.33, 95%CI: 1.04-1.72, <i>p</i> = 0.025) and dominant models (AA+CA/CC: OR= 1.38, 95%CI: 1.00-1.91, <i>p</i> = 0.047). However, in recessive, homozygous, and heterozygous models, no significant difference was observed (all <i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>The <i>VEGF</i>-2578C/A polymorphism is associated with susceptibility to T2DR. In particular, allele A and genotype AA+CA at the <i>VEGF</i>-2578C/A locus were significantly associated with an increased risk of T2DR.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normalized Reflectivity of Middle Limiting Membrane on SD-OCT: A Measure of Acute Ischemia in CRVO.","authors":"Sebastian Bemme, Sheila Büchel, Hans Hoerauf, Nicolas Feltgen, Christian van Oterendorp","doi":"10.1080/02713683.2025.2452941","DOIUrl":"https://doi.org/10.1080/02713683.2025.2452941","url":null,"abstract":"<p><strong>Purpose: </strong>To validate the quantification of the prominent middle limiting membrane (PMLM) sign, a marker of mild-to-moderate acute ischemic damage on optical coherence tomography (OCT), by measuring middle limiting membrane (MLM) reflectivity in patients with central retinal vein occlusion (CRVO) and to investigate the prognostic impact of this measure.</p><p><strong>Methods: </strong>Spectral Domain (SD)-OCT B-scans of 30 patients with CRVO, either sole CRVO or combined central retinal artery and vein occlusion (CCRAVO), were analyzed retrospectively and graded as PMLM present or absent. Normalized MLM reflectivity was calculated as a ratio of the maximum reflectivity within a MLM target layer and the average reflectivity of the retinal pigment epithelium (RPE).</p><p><strong>Results: </strong>Discrimination between OCT B-scans graded as PMLM present or absent based on ROC analysis was far superior with normalized MLM reflectivity (AUC >0.90) than using MLM reflectivity without normalization (AUC = 0.70). Normalized MLM reflectivity was significantly higher in study eyes than healthy fellow eyes as well as in patients with CCRAVO versus sole CRVO. Although normalized MLM reflectivity correlated significantly with baseline and outcome BCVA, we found no significant difference comparing patients with ischemic CRVO versus non-ischemic CRVO.</p><p><strong>Conclusions: </strong>Quantifying MLM reflectivity requires normalization due to shadowing, which is usually caused by retinal haemorrhages in CRVO. RPE reflectivity proved to be a suitable reference layer. Greater normalized MLM reflectivity as a measure of mild-to-moderate acute ischemic damage indicated a worse prognosis regarding visual function in CRVO, however, it allows no risk assessment regarding the development of capillary non-perfusion or anterior segment neovascularization.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computed Tomography - Dacryocystography Guided Management of Traumatic Secondary Acquired Lacrimal Duct Obstruction: SALDO Update Study (SUP) - Paper V.","authors":"Ayushi Agarwal, Mohammad Javed Ali, Nandini Bothra","doi":"10.1080/02713683.2025.2452914","DOIUrl":"https://doi.org/10.1080/02713683.2025.2452914","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the role of computed tomography-dacryocystography (CT-DCG) in the management of traumatic secondary acquired lacrimal duct obstruction (SALDO) and study its correlation with the intra-operative findings.</p><p><strong>Methods: </strong>Retrospective interventional case series. Eighty-five lacrimal drainage systems (LDS) of 79 patients diagnosed with traumatic SALDO, who underwent pre-operative CT-DCG, between January 2019 and June 2023, were analyzed. The lacrimal intervention included endoscopic dacryocystorhinostomy (En-DCR), external DCR(Ex-DCR), or dacryocystectomy (DCT) based on the clinical presentation, CT-DCG findings, local and systemic factors. Anatomical and functional outcomes were assessed.</p><p><strong>Results: </strong>Eighty-five LDS of 79 patients with a mean age of 32 years and male predominance (<i>n</i> = 70, 89%) were studied. The median time of trauma to clinical presentation was 12 months and the duration of epiphora was 8 months. Naso-orbito-ethmoid fractures were seen in 56 (66%) cases and cribriform plate fracture in 5 (6%) patients. CT-DCG revealed a dilated sac in 60 (71%) LDS, shrunken in 13 (15%), while the sac could not be visualized in 3 (4%) LDS. Relative lacrimal sac displacement was seen in 51 (64%) LDS of which 21 (41%) were displaced posteriorly, 18 (35%) superiorly, 5 (10%) inferiorly, and 8 (16%) into the anterior orbit. Sac - duct junction was the most common location of obstruction (<i>n</i> = 78, 92%). At a mean follow up period of 3.5 months, 62 of the 63 LDS surgeries performed (98%) demonstrated anatomical and functional success. Of the 63 operated LDS, CT-DCG findings corroborated with intra-operative findings in 60 (95%) LDS.</p><p><strong>Conclusion: </strong>CT-DCG helps decide the surgical approach, possible complications, intra-operative course and hence has the potential to influence the outcomes. A thorough understanding of CT-DCG, therefore, should be a part of a Dacryologist's armamentarium for managing complex SALDO.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Retinal and Choroidal Microvascularity in Patients with Benign Essential Blepharospasm.","authors":"Husna Topcu, Fatma Poslu Karademir, Mehmet Goksel Ulas, Burcu Kemer Atik, Ayse Cetin Efe, Kubra Serefoglu Cabuk","doi":"10.1080/02713683.2025.2451612","DOIUrl":"https://doi.org/10.1080/02713683.2025.2451612","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the retinal and choroidal microvascular changes in patients with benign essential blepharospasm (BEB) and to investigate the factors that may be effective on microvascularity.</p><p><strong>Methods: </strong>This study included patients with BEB and healthy controls. All participants underwent a comprehensive examination followed by optical coherence tomography angiography (OCTA). Macular vascular perfusion density (VPD), foveal avascular zone (FAZ), and subfoveal choroidal thickness (CT) were measured. The clinical findings of the BEB group and the administration of botulinum toxin A (BTx-A) prior to OCTA imaging were recorded retrospectively from the medical records.</p><p><strong>Results: </strong>A total of 16 patients with BEB and 20 healthy controls were included in this study. VPD values in all quadrants of the superficial and deep macular capillary plexus were significantly different in the BEB group compared to the control group (each <i>p</i>-value <0.05). In the choriocapillaris layer, VPD was significantly higher in the superior, nasal, and temporal quadrants compared to the control group (each <i>p</i>-value <0.05). The BEB group exhibited significantly smaller superficial and deep FAZ values compared to the control group, consistent with the observed increase in vascular density (each <i>p</i>-value <0.05). CT was thinner in the BEB group, although not statistically significant (<i>p</i> = 0.138). No correlation was found between the total dose of BTx-A administered to the BEB group and OCTA parameters (each <i>p</i>-value >0.05).</p><p><strong>Conclusion: </strong>Benign essential blepharospasm may be associated with increased retinal vascular density in the superficial and deep capillary plexus and decreased FAZ area.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence.","authors":"Shichao Han, Wei Zhu, Qianqian Guo","doi":"10.1080/02713683.2025.2452919","DOIUrl":"https://doi.org/10.1080/02713683.2025.2452919","url":null,"abstract":"<p><strong>Purpose: </strong>Pterygium is a common ocular surface disease characterized by a high recurrence rate and unknown etiology.</p><p><strong>Methods: </strong>In this study, we investigated the upregulation of matrix metalloproteinase genes, including MMP1, MMP2, MMP3, MMP7, MMP9, MMP11, MMP12, MMP13, MMP23B, and MMP28, in pterygium tissue using RNA sequencing, Western blotting, and immunohistochemistry.</p><p><strong>Results: </strong>Employing the MEME tool, we identified a conserved DNA motif within the promoter regions of these matrix metalloproteinase genes. Mass spectrometry analysis revealed an interaction between the cohesin complex and this motif. Disrupting the cohesin complex through RNA interference of RAD21 cohesin complex component or structural maintenance of chromosomes 3 in primary pterygial fibroblasts led to decreased matrix metalloproteinase gene expression and reduced recruitment of twist family bHLH transcription factor 1 and transcription factor 4 to matrix metalloproteinase gene promoters.</p><p><strong>Conclusion: </strong>Overall, our findings suggest a novel epigenetic mechanism regulating matrix metalloproteinase transcription in pterygium.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Gamma-Secretase Inhibition by CHF5074 Attenuates Inflammation and Neovascularization in a Murine Model of Choroidal Neovascularization.","authors":"Fei Wang, Bohui Yang, Yuefeng Liao, Mingwei Zhao","doi":"10.1080/02713683.2024.2445656","DOIUrl":"https://doi.org/10.1080/02713683.2024.2445656","url":null,"abstract":"<p><strong>Purpose: </strong>Chronic inflammation plays an important role in the pathogenesis of choroidal neovascularization (CNV). This study aimed to investigate the effect of the CHF5074, a γ-secretase inhibitor, on angiogenesis in a laser-induced CNV model and elucidate its possible molecular mechanism.</p><p><strong>Methods: </strong>Male C57/BL6J mice aged between 6 to 8 weeks were employed to set up a laser-induced model of CNV. Then, CHF5074 was injected intraperitoneally on the day after laser modeling, as well as on the second, third, and fourth days. Immunofluorescence staining was used to evaluate the retinal and choroidal complex. The markers used were CD31 for neovascularization and IBA1 for microglia staining in ocular tissue slices. Fundus fluorescein angiography on days 3d, 7d, and 14d analyzed neovascularization and leakage areas. Inflammatory indicators were examined by Western blot (WB) and enzyme-linked immunosorbent assay (ELISA). High-throughput whole-tissue sequencing of retinal choroids identified relevant cell pathways. Key regulatory factors modulated by CHF5074 were identified <i>via</i> WB. Co-culture of BV2 cells and human umbilical vein endothelial cells (HUVEC) were used to explore the function of CHF5074 on the inhibition of tube formation.</p><p><strong>Results: </strong>CHF5074 significantly decreased CD31 expression in the choroid on 3d, 7d, and 14d post-laser modeling (<i>p</i> < 0.05) and decreased both neovascularization and leakage areas (<i>p</i> < 0.05). Additionally, CHF5074 significantly lowered TNF-α, IL-10, and IL-1β expression levels in the choroid (<i>p</i> < 0.05), as demonstrated by WB analysis and ELISA. High-throughput whole-tissue sequencing identified P38-MAPK, JNK, and Wnt signaling pathways associated with neovascularization. CHF5074 decreased P38 protein phosphorylation (<i>p</i> < 0.05) as confirmed by WB analysis. CHF5074 inhibited the tube formation of HUVECs co-cultured with LPS and ATP-treated BV2 cells.</p><p><strong>Conclusion: </strong>CHF5074 significantly suppresses angiogenesis in laser-induced choroidal neovascularization models, suggesting its potential as a novel agent for preventing and treating CNV.</p>","PeriodicalId":10782,"journal":{"name":"Current Eye Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.7,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}