Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

{"title":"GasPak plus versus anaerocult a — two carbon dioxide/hydrogen systems for cultivation of anaerobes","authors":"Wolfgang R. Heizmann ,&nbsp;Herbert Werner","doi":"10.1016/S0176-6724(89)80022-7","DOIUrl":"10.1016/S0176-6724(89)80022-7","url":null,"abstract":"<div><p>Two disposable carbon dioxide/hydrogen gas-generating systems (GasPak Plus and Anaerocult A) were compared by assessing growth of obligate anaerobic bacteria. Eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 Anaerocult A and 2 GasPak Plus systems. Bacterial growth was expressed as colony-forming units per ml (CFU/ml), reproducibility of the seeding procedure was checked, and the potential interference of H₂S-producing bacteria with operation of the carbon dioxide/hydrogen systems was investigated. The presence of H₂S only inhibited bacterial growth in the case of <em>Peptostreptococcus asaccharolyticus</em> — an effect observed in both systems. Reproducibility of the seeding procedure using the spiral plater was within acceptable range. Differences between the systems were not apparent when comparing total CFU/ml of a given species and the systems therefore provide equally effective environments for incubation of anaerobic bacteria. In both systems, however, growth varied from one species or strain to another. In mixed infections, detection of certain species of anaerobes may therefore be difficult using either system.</p></div><div><p>Zwei Einmalsysteme (GasPak Plus und Anaerocult A) zur Erzeugung einer anaeroben Atmosphäre wurden hinsichtlich der Wachstumsausbeute anaerober Bakterien miteinander verglichen. Die 80 Teststämme (28 Spezies verschiedener Körperregionen) wurden mittels eines Spiralplaters auf vier Hirn-Herz-Kochblutplatten ausgesät und jeweils zwei Platten im Anaerocult-A- bzw. im GasPak-Plus-System bebrütet. Neben dem Parameter der koloniebildenden Einheiten pro ml (KBE/ml) wurde die Reproduzierbarkeit der Ergebnisse des Spiralplaters und der Einfluß H₂S-produzierender Bakterien untersucht. Nur bei <em>Peptostreptococcus asaccharolyticus</em> kam es in Gegenwart von H₂S in beiden Systemen zu einer Verminderung der KBE/ml. Die Reproduzierbarkeit der Ergebnisse des Spiralplaters war gut. Beim Vergleich der Gesamtzahl der KBE/ml konnten zwischen den beiden Systemen keine Unterschiede festgestellt werden, so daß beide Systeme als äquivalent zu betrachten sind. Die unterschiedliche Bandbreite der KBE/ml, sowohl auf Speziesebene als auch der Stämme innerhalb einer Spezies, wies auf eine Inhomogenität des Wachstums hin. Möglicherweise kann hierdurch bei Mischkulturen die Isolierung einzelner Anerobier erschwert sein.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 511-516"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80022-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13642764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lectinophagocytosis mediated by bacterial surface lectins","authors":"Itzhak Ofek","doi":"10.1016/S0176-6724(89)80015-X","DOIUrl":"10.1016/S0176-6724(89)80015-X","url":null,"abstract":"<div><p>The evidence showing that non-opsonic recognition of bacteria by phagocytes involve interaction between bacterial surface lectin and sugars on the phagocytic cells is summarized. This process, termed lectinophagocytosis, probably occur in vivo as evident from experimental infections with mixed phenotypes one of which express mannose specific (MS) lectin which mediate lectinophagocytosis of the bacteria and the other does not. In all cases studied, the lectin bearing phenotype survived better in phagocytes-poor sites and the phenotype which does not express this lectin survived better in phagocytes-rich sites. Due to the phase variation phenomenon, an off-on switch allowing the bacterial clone to alternate between lectin expressing and non-expressing phenotypes, the invading bacteria grow as a mixture of phenotypes. The phenotype expressing fimbrial lectin for which receptors are accessible on phagocytic cells undergo lectinophagocytosis. The phenotypes not expressing fimbrial lectin or expressing lectin for which receptors are not available on phagocytic cells may escape phagocytosis and proliferate. It is postulated that pathogenesis of inflammation and tissue damage following infections with MS lectin bearing bacteria may be partly due to both bacterial proliferation resulting in the release of toxic products and to lectinophagocytosis associated with the release of inflammatory agents.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 449-455"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80015-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13793675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demonstration of capsules in Clostridium difficile","authors":"Elke Strelau ,&nbsp;Barbara Wagner ,&nbsp;Manfred Wagner ,&nbsp;Wolfgang Karsch","doi":"10.1016/S0176-6724(89)80016-1","DOIUrl":"10.1016/S0176-6724(89)80016-1","url":null,"abstract":"<div><p>In four strains of <em>Clostridium difficile</em> the formation of capsules was demonstrated by light and electron microscopy.</p></div><div><p>Bei vier Stämmen von <em>Clostridium difficile</em> wurde durch Licht- und Elektronenmikroskopie eine Kapselbildung nachgewiesen.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 456-461"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80016-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13851473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The significance of the Ipazyme IgA and IgG antibody test in the diagnosis of urogenital chlamydial infections","authors":"Helmut Näher ,&nbsp;Detlef Petzoldt","doi":"10.1016/S0176-6724(89)80005-7","DOIUrl":"10.1016/S0176-6724(89)80005-7","url":null,"abstract":"<div><p>Urogenital specimens from 200 male and female patients were cultivated for the detection of <em>C. trachomatis</em>. For comparison, serum of the same patients was investigated with the Ipazyme IgA and IgG test. This comparison of culture and serological tests revealed a sensitivity of the IgA Ipazyme test of 56% and a specificity of 81%. For the IgG Ipazyme test, the corresponding values were 77% and 39%. The sensitivity of the conventional immunofluorescence test reached 46% and its specificity was 50% for the same group of patients. Antibiotic treatment of 13 IgA-positive patients resulted in a significant decrease of the titre in only one case. The introduction of the Ipazyme test does not open a new aspect in chlamydial serology, i.e. the diagostic value of serology for the detection of a current chlamydial infection remains low.</p></div><div><p>Das Ergebnis der Anzüchtung von <em>C. trachomatis</em> aus urogenitalem Abstrichmaterial von 200 Patienten und Patientinnen wurde mit dem Ergebnis des Ipazyme IgG- und IgA-Antikörpertests bei der Untersuchung der zugehörigen Seren verglichen. Für den Ipazyme IgA-Test wurde eine Sensitivität von 56% ermittelt, die Spezifität lag bei 81%. Die entsprechenden Parameter betrugen für den Ipazyme IgG-Test 77% bzw. 39%. Zum Vergleich: Die Sensititivät des konventionellen IgG-IFT lag bei der identischen Klientel bei 46% und die Spezifität bei 50%. Eine antibiotische Therapie bei 13 IgA-positiven Patienten führte innerhalb von 3–11 Monaten nur in einem Fall zu einem signifikanten Titerabfall. Durch die Einführung des Ipazyme-Tests ergibt sich kein neuer Aspekt für die Chlamydiendiagnostik, d.h. daß die Serologie nach wie vor für die Diagnose einer bestehenden urogenitalen Chlamydieninfektion nur eine untergeordnete Rolle spielt.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 373-378"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80005-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13790804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative assessment of human neutrophil chemiluminescence induced by opsonized Escherichia coli K-12","authors":"Thomas W. Jungi ,&nbsp;Anton Schmid ,&nbsp;Andreas Morell ,&nbsp;Peter J. Spaeth ,&nbsp;Ernst Peterhans","doi":"10.1016/S0176-6724(89)80010-0","DOIUrl":"10.1016/S0176-6724(89)80010-0","url":null,"abstract":"<div><p>The interaction of opsonized <em>E. coli</em> K-12 bacteria and polymorphonuclear leukocytes (PMN) was quantified, using luminol-enhanced chemiluminescence (CL) as a parameter of PMN stimulation. On a double-logarithmic scale light emission depended on the opsonin concentration used during pre-opsonisation. The most potent CL-inducing agent was fresh human serum, and its stimulatory activity depended on an intact complement (C) system. Both inactivation of C by heating or blocking the classical C pathway with EGTD decreased the CL-inducing potency by a factor of 8 to 16. Hypogammaglobulinemic heated serum mediated little CL. IgG for intravenous use mediated CL generation, but reduction/alkylation and sulphitolysis reduced the stimulatory power. Evidence is presented that the anti-K-12 antibodies within commercial IgG and IgM used for substitution do not improve the stimulatory power of IgG-deficient, IgM- and C-sufficient serum, unless very high Ig concentrations are substituted.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 406-417"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80010-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13790806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mikrobielle besiedlung kariöser progressionsstadien im dentin menschlicher zähne — eine kontrollierte therapiestudie","authors":"Susanne Kneist,&nbsp;Roswitha Heinrich,&nbsp;W. Künzel","doi":"10.1016/S0176-6724(89)80007-0","DOIUrl":"10.1016/S0176-6724(89)80007-0","url":null,"abstract":"<div><p>In a controlled clinical trial the microflora of the cavity floor of 70 primary lower second molars with deep carious lesions was determined after caries excavation. The teeth were extracted and pulpal status was evaluated after 16 month of microbial control to determine the etiopathogenic role of germs for carious progression in dentine.</p><p>67% of the primary molars were free from pulpal inflammations. Soft carious dentine were significantly higher infected than the clinically acceptable hard dentine. Only in 40% of the cavity floors the microorganisms were eliminated. In the infected teeth basophilic microorganisms were found in causality to pulps without inflammations; acidogenic streptococci and lactobacilli were involved in pulpal inflammations. Results indicate that the latter genera of microorganisms are of etiological significance for carious progression in dentine.</p></div><div><p>In einer klinisch-kontrollierten Therapiestudie zur Vitalerhaltung des Milchzahnendo-dontes wurde die Mikroflora am Kavitätenboden von 70 zweiten unteren Milchmolaren mit symptomloser Caries profunda nach Kariesexkavation erfaßt und ihre ätiopathogenetische Bedeutung für die Kariesprogression in das Dentin durch nachfolgende morphologische Untersuchungen der Zähne nach einem Therapiezeitraum von 16 Monaten bewertet. 67% der Milchmolaren zeigten einen entzündungsfreien Pulpastatus.</p><p>Geringgradig erweichtes Dentin erwies sich im Vergleich zu klinisch akzeptablen harten Dentin des Kavitätenbodens als deutlich höher besiedelt und nur 40% der Kavitätenboden waren nach mikrobiologischer Kontrolle befundfrei.</p><p>In den noch geringgradig infizierten Zähnen korrelierte aber eine entzündungsfreie Pulpa mit einem basophilen Besiedlungsmuster und acidogene und acidurische Streptokokken (<em>S. mutans</em>) und Laktobazillen wurden bei chronischen Pulpainflammationen aufgefunden, so daß letztere Gattungen von Mikroorganismen ätiopathogenetish für die kariöse Progression in das Dentin bedeutungsvoll sein dürften.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 385-395"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80007-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121357862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity testing of listeria strains isolated from food in fertilized hen's eggs","authors":"Christine Lattmann ,&nbsp;Andreas Schwarzkopf ,&nbsp;Heinz P.R. Seeliger","doi":"10.1016/S0176-6724(89)80009-4","DOIUrl":"10.1016/S0176-6724(89)80009-4","url":null,"abstract":"<div><p>Pathogenicity testing of 10-day-old fertilized hen's eggs infected with <em>Listeria</em> species demonstrated that <em>L. monocytogenes</em> isolated from cheese and human samples caused death in 100% of the injected chicken embryos within 96 h. In contrast, <em>L. innocua</em> isolated from cheese was fatal for only 17% of the infected embryos. All embryos infected with <em>L. seeligeri</em> survived. The results confirmed statements of previous authors about the pathogenicity of <em>L. monocytogenes</em>, regardless of its origin.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 400-405"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80009-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13641257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of toxic shock syndrome toxin-1 production among staphylococcus aureus strains and clinical implications","authors":"A. Satyanarayan Naidu ,&nbsp;Carl Kamme,&nbsp;Åsa Ljungh,&nbsp;Torkel Wadström","doi":"10.1016/S0176-6724(89)80002-1","DOIUrl":"10.1016/S0176-6724(89)80002-1","url":null,"abstract":"<div><p>Among 250 <em>S. aureus</em> clinical isolates, the incidence of TSST-1 production was 18.0%. <em>S. aureus</em> var. <em>hominis</em> strains were predominant (95.6%), producing high levels of toxin <em>in vitro</em>, within the range of 0.6 to 4.3 μg/ml and exhibiting crystal violet binding with C/D pattern. No correlation was found between the level of TSST-1 production <em>in vitro</em> and the clinical course. Two (3.4%) of the var. <em>bovis</em> strains produced toxin in amounts less than 0.6 μg/ml and did not bind crystal violet. None of the 24 var. <em>canis</em> isolates produced TSST-1. Fifty five per cent of the isolates of phage group I produced TSST-1 and corresponded to 57.8% of the toxigenic strains. Two of the 250 patients developed toxic shock syndrome.</p></div><div><p>Die Häufigkeit der Bildung von TSST-1 bei 250 klinischen Isolaten von <em>S. aureus</em> betrug 18,0%. Es herrschten <em>S. aureus</em> var. <em>hominis</em>-Stämme vor (95,6%), die <em>in vitro</em> hohe Toxin-spiegel (Bereich 0,6–4,3 μg/ml) bildeten und Kristallviolettbildung mit einem C/D-Muster zeigten. Es wurde keine Korrelation zwischen der Höhe der TSST-1-Bildung <em>in vitro</em> und dem klinischen Verlauf festgestellt. Zwei (3,4%) der var <em>bovis</em>-Stämme bildeten Toxin in Mengen, die unter 0,6 μg/ml lagen und Kristallviolett nicht banden. Keines der 24 var. <em>canis</em>-Isolate bildete TSST-1. 55% der Isolate der Phagengruppe I bildete TSST-1, was 57,8% der toxigenen Stämme entsprach. Bei zwei der 250 Patientinnen entwickelte sich ein “Toxic Shock”-Syndrom.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 337-344"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80002-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13790803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duck hepatitis B virus: Cloning and subcloning of the viral genome","authors":"Konrad Oexle,&nbsp;Hubert E. Blum ,&nbsp;Eike Walter,&nbsp;Wolf-Bernhard Offensperger,&nbsp;Silke Offensperger,&nbsp;Hajo Grundmann,&nbsp;Karl Teubner,&nbsp;Wolfgang Gerok","doi":"10.1016/S0176-6724(89)80012-4","DOIUrl":"10.1016/S0176-6724(89)80012-4","url":null,"abstract":"<div><p>In the course of studies on the biology of hepadnavirus infections, duck hepatitis B virus (DHBV) DNA was isolated from the serum of a German Pekin duck. Viral DNA was cloned in <em>E. coli</em> using pBR 322 DNA as a vector. The cloned DHBV DNA F 12 was characterised by restriction enzyme analyses.</p><p>DHBV DNA F 1–6 was subcloned in both orientations in plasmid pSP 65 to produce strand-specific RNA probes. These probes specifically identified asymmetrically replicating nascent minus-strand DHBV DNA species or plus-strand viral RNA transcripts.</p></div><div><p>Im Rahmen von Studien zur Biologie der Hepadnavirus-Infektionen wurde Entenhepatitis B Virus (DHBV) DNA aus einer deutschen Pekingente isoliert. Mit Hilfe des Vektors pBR 322 wurde die virale DNA in <em>E. coli</em> kloniert. Die klonierte DHBV DNA F 1–6 wurde durch Restriktionsanalysen charakterisiert.</p><p>Zur Herstellung von strang-spezifischen RNA Gensonden wurde DHBV DNA F 1–6 im Plasmid pSP 65 in beiden Orientierungen subkloniert. Mit diesen RNA Gensonden wurden naszierende Minusstränge der sich asymmetrisch replizierenden DHBV DNA bzw. virale RNA Transkripte von Plusstrang-Polarität spezifisch nachgewiesen.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 424-433"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80012-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14063915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation on monoclonal antibodies against two serovars of the icterohaemorrhagiae serogroup of leptospira","authors":"Zhang Wan-He ,&nbsp;Bai Xiu-Fen,&nbsp;Deng Qing-Dong,&nbsp;Nie Di-Kai","doi":"10.1016/S0176-6724(89)80001-X","DOIUrl":"10.1016/S0176-6724(89)80001-X","url":null,"abstract":"<div><p>By means of the cell fusion technique, two hybridoma cell lines, V-1 and H₂-1 have been obtained. V-1 cells secrete monocloncal antibody against serovars <em>icterohaemorrhagiae</em> and dakota. The H₂-1 cell line secretes serovar-specific monoclonal antibody against serovar H₂.</p><p>These monoclonal antibodies have been successfully used in serovar-typing of leptospires isolated in China. The results of identification of leptospires by using monoclonal antibodies showed total coincidence with that by the traditional cross agglutinin absorption test and factor antiserum method.</p><p>It was confirmed by using monoclonal antibody that the serological agglutination totally paralleled with animal protection. On the basis of the study, a concept was proposed that the agglutination in vitro and the protection in vivo are different manifestations in different reaction systems from the same antibody (antibodies) stimulated by a component(s) of the surface antigen of leptospires.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 329-336"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80001-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116173420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}