Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

{"title":"Antigenic analysis of vibrio cholerae O1 by crossed immunoelectrophoresis","authors":"Shahjahan Kabir","doi":"10.1016/S0176-6724(89)80004-5","DOIUrl":"10.1016/S0176-6724(89)80004-5","url":null,"abstract":"<div><p>Antigens from <em>Vibrio cholerae</em> O1 were analyzed by crossed immunoelectophoresis (CIE) using sera from immunized rabbits. Thirty different anode-migrating antigens were detected in sonicated antigen preparations of <em>V. cholerae</em>. These antigens were numbered in order to establish a reference precipitation pattern. Antigen no. 30 was identified as the lipopolysaccharide (LPS) antigen, because it reacted with (i) periodic acid-Schiff (PAS) reagent and (ii) the affinity-purified anti-LPS antibodies. Treatment with proteinase K demonstrated that most of the precipitation lines were due to proteins, a part of which were localised at the cell surface. The major outer membrane protein was found to be closely associated with the precipitation line due to the LPS (antigen no. 30). The antigenicity and immunogenicity of <em>V. cholerae</em> cells killed by different methods (merthiolate, heat, phenol, formalin) were examined. As determined by CIE, killing with merthiolate preserved most of the major components of <em>V. cholerae</em>. Heat, phenol and formalin altered the antigenic mosaic of <em>V. cholerae</em>. These results suggested that CIE can be used to analyze several aspects of <em>V. cholerae</em> antigens.</p></div><div><p>Antigene von <em>Vibrio cholerae</em> O1 wurden unter Verwendung von Kaninchenimmunseren mit der gekreuzten Immunelektophorese (CIE) untersucht. In ultraschallbehandelten Antigenpräparationen wurden 30 verschiedene Antigene ermittelt. Ein Antigen wurde als Lipopolysaccharidantigen identifiziert. Die meisten anderen Präzipitationslinien entsprechen Proteinantigenen, von denen ein Teil an der Oberfläche lokalisiert ist. Die Antigenität, Immunogenität und die Hämagglutinationsaktivität von <em>Vibrio cholerae</em>-Zellen, die mit verschiedenen Methoden (Hitze, Merthiolat, Phenol, Formalin) abgetötet worden waren, wurde vergleichend untersucht. Mit der CIE und der isoelektrischen Fokussierung wurde festgestellt, daß die Abtötung mit Merthiolat die Hauptkomponenten von <em>V. cholerae</em> am besten bewahrt. Hitze, Phenol und Formalin alterieren sowohl das Antigen-mosaik wie auch die Hämagglutinationsaktivität von <em>V. cholerae</em>.</p><p>Diese Ergebnisse zeigen, daß die CIE für die Analyse von Choleraantigen geeignet ist.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 361-372"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80004-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14065583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of borrelia burgdorferi in Ixodes ricinus ticks in Berlin (West)","authors":"Olaf Kahl ,&nbsp;Katrin Schmidt ,&nbsp;Arno Schönberg ,&nbsp;Ulrich Laukamm-Josten ,&nbsp;Wilhelm Knülle ,&nbsp;Ulrich Bienzle","doi":"10.1016/S0176-6724(89)80013-6","DOIUrl":"10.1016/S0176-6724(89)80013-6","url":null,"abstract":"<div><p>In 1986, 1711 nymphal and adult <em>Ixodes ricinus</em> originating from Berlin (West) forests were examined individually or in pools of up to 10 ticks for the presence of <em>Borrelia burgdorferi</em>, the etiologic agent of Lyme borreliosis. Detection of borreliae was carried out by means of a culture method using modified Barbour-Stoenner-Kelly-Medium (BSK II).</p><p>Tick populations from 14 out of 15 locations contained positive specimens. The calculated minimal infection rate of pooled ticks was 2.5% in nymphs (n = 1365), 10.2% in females (n = 59), and 5.3% in males (n = 114). Among those ticks examined individually, none of the nymphs (n = 49) proved to be positive but <em>B. burgdorferi</em> was isolated from 8.2% of the females (n = 73) and 7.8% of the males (n = 51). Fifty-five out of 56 isolates were identified as <em>B. burgdorferi</em> by means of an indirect immunofluorescence test (IFT) using monoclonal antibody H 5332.</p><p>From these results <em>B. burgdorferi</em> must be considered as being present in the Berlin area.</p></div><div><p>Im Jahre 1986 wurden 1711 <em>Ixodes ricinus</em> (Nymphen und Adulte) aus Berlin (West) einzeln oder in Gruppen von maximal 10 Zecken auf <em>Borrelia burgdorferi</em>, dem Erreger der Lyme-Borreliose, untersucht. Der Borreliennachweis erfolgte kulturell in modifiziertem Barbour-Stoenner-Kelly-Medium (BSK II).</p><p>In 14 von 15 aufgesuchten Forstlokalitäten wurden positive Zecken gefunden. Die minimale Durchseuchungsrate der in Gruppen untersuchten Zecken betrug bei Nymphen 2,5% (n = 1365), bei Weibchen 10,2% (n = 59) und bei Männchen 5,3% (n = 114). Von einzeln untersuchten Zecken waren alle Nymphen (n = 49) negativ, jedoch wurde aus 8,2% der Weibchen (n = 73) und aus 7,8% der Männchen (n = 51) <em>B. burgdorferi</em> isoliert. Fünfundzwanzig von insgesamt 56 Isolaten wurden mit einem indirekten Immunofluoreszenztest (IFT) unter Verwendung von monoklonalen Antikörpern H 5332 als <em>B. burgdorferi</em> identifiziert.</p><p>Auf Grund dieser Ergebnisse muß damit gerechnet werden, daß <em>B. burgdorferi</em> im gesamten Berliner Raum in <em>I. ricinus</em> vorkommt.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 434-440"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80013-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14063916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ability of smooth and rough variants of mycobacterium avium and M. intracellulare to multiply and survive intracellularly: role of C-mycosides","authors":"Nalin Rastogi ,&nbsp;Veronique Levy-Frebault,&nbsp;Marie-Christine Blom-Potar,&nbsp;Hugo L. David","doi":"10.1016/S0176-6724(89)80003-3","DOIUrl":"10.1016/S0176-6724(89)80003-3","url":null,"abstract":"<div><p>A spontaneous rough (Rg) mutant of <em>M. avium</em> ATCC 15769 was mutagenized using N-methyl-N-nitro-nitrosoguanidine (MNNG). Out of 54 clones initially chosen on the basis of their morphological appearance on the 7H11 agar, seven Rg clones were selected on the basis of their response to current biochemical tests, and were later characterized for their cell wall amphiphilic contents (analysis of loosely-bound surface lipids for mycosides, peptidolipids, phospholipids, and mycolic acids by thin layer chromatography, and fatty acids by gas chromatography), and ability to grow intracellularly inside J-774 macrophages. A parallel study was also performed on a previously reported Rg mutant of <em>M. intracellulare</em> serovar 20 (<em>W. W. Barrow</em> and <em>P. J. Brennan</em>, J. Bact. 150 (1982) 381–384) which lacked the ability to synthesize mycosides C (MYC⁻). The results obtained were compared to parental smooth (Sm) colony-types of the respective <em>M. avium-intracellulare</em> strains. Our results showed that neither the ninhydrin-reacting lipids (probably peptidolipids) nor the glycopeptidolipids (mycosides C) were primary factors responsible for the intracellular survival and multiplication of these bacteria. Ultrastructural studies showed that although the polysaccharide-rich outer wall layer (POL) in case of MYC⁻ Rg strain was not uniformly distributed and contained blebs, these bacteria formed the characteristic electron-transparent zone (ETZ) upon phagocytosis by macrophages. Furthermore, the multiplication of MYC⁻ Rg strain inside macrophages did not result in intracellular selection of MYC⁺ bacteria, nor in Rg to Sm transition.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 345-360"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80003-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14065582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and significance of clostridium difficile in faecal specimens of hospitalized children","authors":"Wolfgang Karsch ,&nbsp;Elke Strelau,&nbsp;Wolf-Dieter Grahlow,&nbsp;Evelin Fischer,&nbsp;Rotraud Schulz","doi":"10.1016/S0176-6724(89)80014-8","DOIUrl":"10.1016/S0176-6724(89)80014-8","url":null,"abstract":"<div><p>Stool specimens from 766 hospitalized children, 418 with diarrhoea and 348 controls, were investigated for <em>C. difficile</em>. In both groups the rate of isolation was highest (about 30%) during the first year of life, dropping to nearly 5% in older children. There was no significant difference in the frequency of <em>C. difficile</em> in children with diarrhoea and the controls nor was there a significant influence of previous antibiotic therapy on the rate of isolation. 111/135 strains (82.2%) produced toxin B and 58/135 strains (43%) produced toxin A measured by Y-1- cell culture (toxin B) and rabbit ileal loop test (toxin A). We did not find any significant difference in the toxin production between strains isolated from diarrhoeal children and from the controls. A total of 285 stool specimens was investigated for toxin B production in vivo. There was no significant difference of toxin B in the stool specimens of both groups.</p></div><div><p>Stuhlproben von 766 hospitalisierten Kindern, 418 mit Enteritis und 348 Kontrollen, wurden auf <em>C. difficile</em> untersucht. In beiden Gruppen war die Isolierungrate mit ca. 30% am höchsten im ersten Lebensjahr und fiel auf ca. 5% bei älteren Kindern. Die Isolierungshäufigkeit zeigte in beiden Gruppen keinen signifikanten Unterschied. In beiden Gruppen wurde kein signifikanter Einfluß einer vorherigen antibiotischen Therapie auf die Isolierungsrate von <em>C. difficile</em> festgestellt. 111/135 Stämmen (82,2%) bildeten Toxin B und 58/135 Stämmen (43%) bildeten Toxin A. Es wurde kein signifikanter Unterschied in der Häufigkeit der Toxinbildung zwischen Stämmen von Kindern mit Enteritis und Stämmen aus der Kontrollgruppe gefunden. In 285 Stuhlproben wurde Toxin B in vivo bestimmt. Es bestand auch hier kein signifikanter Unterschied in der Häufigkeit der Bildung von Toxin B zwischen beiden Gruppen.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 441-448"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80014-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13641258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibodies against toxic shock syndrome toxin No. 1 (TSST-1) in Poland","authors":"Małgorzata Bulanda ,&nbsp;Gerhard Kunstmann ,&nbsp;Gottfried Mauff ,&nbsp;Marian Kurek ,&nbsp;Gerhard Pulverer ,&nbsp;Piotr B. Heczko","doi":"10.1016/S0176-6724(89)80008-2","DOIUrl":"10.1016/S0176-6724(89)80008-2","url":null,"abstract":"<div><p>A total of 174 sera from healthy Polish individuals of different age groups were tested for TSST-1 antibody by the micro-ELISA method. Over 90% of sera contained the antibody with titres equal to above 1:10², even in children and adolescents. The data suggest that the majority of the Polish population acquires TSST-1-antibody during early childhood, whereas a small portion of the population seems to remain anti-TSST-1-negative.</p></div><div><p>Eine Stichprobe von 174 Seren aus der polnischen Bevölkerung wurde im Mikro-ELISA auf das Vorhandensein von TSST-1 Antikörpern untersucht. Bei über 90% der Probanden einchließlich von Kindern und Jugendlichen fand sich ein Antikörpertiter von mindestens 1:10². Die Befunde berechtigen zu der Annahme, daß die überwiegende Mehrheit der Probanden TSST-1-Antikörper bereits in der frühen Kindheit erwirbt, während ein kleiner Anteil der Bevölkerung auch langfristig keine antitoxische Immunität zu erwerben scheint.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 396-399"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80008-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14063913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of neutrophil adherence to toxocara canis larvae by the C3 component of complement and IgG antibodies","authors":"Martin Huwer ,&nbsp;Sabine Sanft,&nbsp;Jabbar S. Ahmed","doi":"10.1016/S0176-6724(89)80011-2","DOIUrl":"10.1016/S0176-6724(89)80011-2","url":null,"abstract":"<div><p>In the present study, the binding of human neutrophils to <em>Toxocara canis</em> larvae was examined in vitro. It was found that IgG and/or the C₃ component of complement were able to enhance the attachment of the cells to the parasites. This was associated with the generation of strong chemiluminescence reactions.</p><p>Despite the binding of the cells, the larvae were able to escape and to maintain their infectivity. This was apparent, since the parasites could survive and migrate into different organs including the brain after having been inoculated into mice.</p></div><div><p>In-vitro-Untersuchungen zur Interaktion neutrophiler Granulozyten vom Menschen mit infektiösen Larven von <em>Toxocara canis</em> wurden durchgeführt. Die Ergebnisse von Zellanlagerungs- und Immunfluoreszenztests zeigen, daß sowohl IgG, als auch die C₃-Komponente des Komplementsystems an der Zellaggregation am Parasiten beteiligt sind. Im Chemilumineszenztest war als Ausdruck der Aktivierung der phagozytischen Zellen eine ausgeprägte Reaktion zu beobachten. Trotz der starken Zellanlagerung überlebten die Larven und behielten ihre Infektiosität. Nach Inokulation in Mäuse wurden die Larven im Gehirn, sowie in verschiedenen anderen Organen gefunden.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 418-423"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80011-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14063914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of group B streptococci in vaginal swabs of parturients by Latex Particle Agglutination","authors":"Jörg E. Hoppe ,&nbsp;Christiane Lindenau,&nbsp;Wolfram Höfler","doi":"10.1016/S0176-6724(89)80006-9","DOIUrl":"10.1016/S0176-6724(89)80006-9","url":null,"abstract":"<div><p>Duplicate high vaginal swabs were obtained from 200 parturient women at Abeokuta (Nigeria). By culture on a selective agar twenty-two women (11%) were found to harbour group B streptococci. The second swab was subjected to a 45 min enzymatic extraction procedure and then tested in a latex agglutination test for group B streptococcal antigen (Wellcogen Strep B; Wellcome Diagnostics, Dartford, UK). The latex test permitted the detection of three out of 22 colonized women (overall sensitivity 13.6%). The sensitivity for detection of heavy colonization was 50% (three out of six women). It is concluded that the sensitivity of the method employed for rapid detection of group B streptococcal antigen in vaginal swabs is still unsatisfactory.</p></div><div><p>Doppelte hohe Vaginalabstriche wurden entnommen bei 200 vor der Geburt stehenden Frauen in Abeokuta (Nigeria). Durch Anzüchtung auf einem Selektivagar wurde Besiedelung mit Gruppe-B-Streptokokken bei 22 Frauen festgestellt (11%). Der zweite Tupfer wurde nach einer 45-minütigen enzymatischen Extraktionsbehandlung mit einem Latex-Agglutinationstest (Wellcogen Strep B; Wellcome Diagnostics, Dartford, Großbritannien) auf das Vorhandensein von Gruppe-B-Streptokokken-Antigen untersucht. Der Latex-Test war positiv bei drei von 22 besiedelten Frauen (Gesamtsensitivität 13,6%). Die Sensitivität für den Nachweis einer starken Besiedelung war 50% (drei von sechs Frauen). Aus den Ergebnissen wird gefolgert, daß die verwendete Methode zum Schnellnachweis von Gruppe-B-Streptokokken-Antigen in Vaginalabstrichen noch nicht ausreichend sensitiv ist.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 3","pages":"Pages 379-384"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80006-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13790805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutanolysin-induced lysis of actinomyces pyogenes determined by aggregometry","authors":"Ch. Lämmler ,&nbsp;Ch. Frede","doi":"10.1016/S0176-6724(88)80066-X","DOIUrl":"10.1016/S0176-6724(88)80066-X","url":null,"abstract":"<div><p>The lytic activity of mutanolysin from <em>Streptomyces globisporus</em> on 42 cultures of <em>Actinomyces pyogenes</em> could be effectively analyzed in an aggregometer. It was expressed as increase of transmittance at 546 nm after 20 min and 2 h at 37°C. The <em>A. pyogenes</em> cultures revealed no uniform lysis pattern. Most of the cultures were lyzed within 20 to 40 min at 37°C, others were lyzed only moderately or weakly within 2 h of incubation. The lytic activity was optimal at low (0.01 mol/1) molarity of the lysis buffer between pH 5.7 and 7 and could be inhibited by HgCl₂. <em>A. pyogenes</em> was not lyzed by lysostaphin or lysozyme.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 447-453"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80066-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex Typing of Methicillin-resistant Staphylococcus aureus (MRSA)","authors":"Wolfgang Witte ,&nbsp;Richard R. Marples ,&nbsp;Judith F. Richardson","doi":"10.1016/S0176-6724(88)80143-3","DOIUrl":"10.1016/S0176-6724(88)80143-3","url":null,"abstract":"<div><p>To discriminate between methicillin-resistant <em>Staphylococcus aureus</em> from 5 nosocomial outbreaks and from sporadic nosocomial infections, the efficacy of a complex typing scheme by phage typing, biochemical typing, resistance phenotype, plasmid profiles, plasmid patterns and attribution of resistance determinants to the chromosome was studied. In addition to the International Basic Set and experimental phages 88–93,10 experimental phages from the Public Health Laboratory Service, Colindale, London, were used for phage-typing. The 10 experimental phages from PHLS in particular, in combination with plasmid profiles and plasmid patterns, were of special discriminative value.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 76-82"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80143-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison between Methods of Identification and Serotyping of Encapsulated Strains of Haemophilus influenzae","authors":"Irene Susanne Taubitz,&nbsp;Henning Brandis","doi":"10.1016/S0176-6724(88)80144-5","DOIUrl":"10.1016/S0176-6724(88)80144-5","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Seven methods of serotyping of &lt;em&gt;Haemophilus influenzae&lt;/em&gt; were evaluated. Comparing slide agglutination, staphylococcal coagglutination, latex agglutination, counterimmunoelectrophoresis, immunofluorescence, capsular swelling, and cultivation on antiserum agar the commercial coagglutination test was most reliable, most rapid, and easiest to perform. To identify all six serotypes this coagglutination test had to be combined with slide agglutination. With most methods best results were achieved by using cultures incubated at 37°C for 6 h. As nonencapsulated strains often agglutinated unspecifically, selection of probably typeable strains was useful. Differentiation with help of colonial morphology and opalescent growth was facilitated by cultivation on Brain Heart Infusion (BHI) Chocolate Agar and testing of growth factor requirements on translucent BHI Agar with strips containing the growth factors V, X, and VX, respectively. In broth turbid growth was a hint for encapsulation. Nigrosin staining, a negative capsule staining, proved to be useful if specific antisera are not available.&lt;/p&gt;&lt;p&gt;From 252 clinical isolates of &lt;em&gt;H. influenzae&lt;/em&gt; 216 were not typeable. 36 strains could be serotyped. 27 (75%) belonged to serotype b, 6 (16.6%) were serotype e, 3 (8.3%) were serotype f. Serotype e and f were most difficult to identify. Spectrum of patients and diseases were corresponding to the findings of other authors. Less well-known infections like cellulitis (erysipelas of the cheeks) and arthritis were observed, too. Rapid identification of at least &lt;em&gt;H. influenzae&lt;/em&gt; type b could render treatment in some cases more effective by early application of a suitable antibiotic.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;p&gt;Sieben Methoden für die Serotypisierung von &lt;em&gt;Haemophilus influenzae&lt;/em&gt; wurden erprobt. Beim Vergleich von Objektträgeragglutination, Staphylokokken-Koagglutination, Latexagglutination, Gegenstromimmunelektrophorese, Immunfluoreszenz, Kapselquellung und Kultivierung auf Antiserumagar erwies sich der kommerzielle Koagglutinationstest als am zuverlässigsten, schnellsten und am einfachsten durchzuführen. Um alle sechs Serotypen zu identifizieren, war bei diesem Koagglutinationstest eine Kombination mit der Objektträgeragglutination notwendig. Bei nahezu allen Methoden wurden beste Ergebnisse bei Verwendung von 6 h lang bei 37°C bebrüteten Kulturen erzielt. Da unbekapselte Stämme häufig unspezifisch agglutinierten, war eine Vorauswahl wahrscheinlich typisierbarer Stämme nützlich. Differenzierung anhand von Koloniemorphologie und -Opaleszenz wurde erleichtert durch Kultivierung auf Brain Heart Infusion (BHI) Chocolate Agar und Wuchsfak-tortestung auf klarem BHI-Agar mit Teststreifen für die Wuchsstoffe. Trübes Wachstum in Bouillon war ein Hinweis auf Bekapselung. Die Nigrosinfärbung, eine negative Kapseldarstellung, erwies sich als nützlich, wenn keine spezifischen Antiseren zur Verfügung standen.&lt;/p&gt;&lt;p&gt;Von 252 klinischen Isolaten von &lt;em&gt;H. influen","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 83-97"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80144-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}