Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

{"title":"Mutanolysin-induced lysis of actinomyces pyogenes determined by aggregometry","authors":"Ch. Lämmler ,&nbsp;Ch. Frede","doi":"10.1016/S0176-6724(88)80066-X","DOIUrl":"10.1016/S0176-6724(88)80066-X","url":null,"abstract":"<div><p>The lytic activity of mutanolysin from <em>Streptomyces globisporus</em> on 42 cultures of <em>Actinomyces pyogenes</em> could be effectively analyzed in an aggregometer. It was expressed as increase of transmittance at 546 nm after 20 min and 2 h at 37°C. The <em>A. pyogenes</em> cultures revealed no uniform lysis pattern. Most of the cultures were lyzed within 20 to 40 min at 37°C, others were lyzed only moderately or weakly within 2 h of incubation. The lytic activity was optimal at low (0.01 mol/1) molarity of the lysis buffer between pH 5.7 and 7 and could be inhibited by HgCl₂. <em>A. pyogenes</em> was not lyzed by lysostaphin or lysozyme.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 447-453"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80066-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison between Methods of Identification and Serotyping of Encapsulated Strains of Haemophilus influenzae","authors":"Irene Susanne Taubitz,&nbsp;Henning Brandis","doi":"10.1016/S0176-6724(88)80144-5","DOIUrl":"10.1016/S0176-6724(88)80144-5","url":null,"abstract":"<div><p>Seven methods of serotyping of <em>Haemophilus influenzae</em> were evaluated. Comparing slide agglutination, staphylococcal coagglutination, latex agglutination, counterimmunoelectrophoresis, immunofluorescence, capsular swelling, and cultivation on antiserum agar the commercial coagglutination test was most reliable, most rapid, and easiest to perform. To identify all six serotypes this coagglutination test had to be combined with slide agglutination. With most methods best results were achieved by using cultures incubated at 37°C for 6 h. As nonencapsulated strains often agglutinated unspecifically, selection of probably typeable strains was useful. Differentiation with help of colonial morphology and opalescent growth was facilitated by cultivation on Brain Heart Infusion (BHI) Chocolate Agar and testing of growth factor requirements on translucent BHI Agar with strips containing the growth factors V, X, and VX, respectively. In broth turbid growth was a hint for encapsulation. Nigrosin staining, a negative capsule staining, proved to be useful if specific antisera are not available.</p><p>From 252 clinical isolates of <em>H. influenzae</em> 216 were not typeable. 36 strains could be serotyped. 27 (75%) belonged to serotype b, 6 (16.6%) were serotype e, 3 (8.3%) were serotype f. Serotype e and f were most difficult to identify. Spectrum of patients and diseases were corresponding to the findings of other authors. Less well-known infections like cellulitis (erysipelas of the cheeks) and arthritis were observed, too. Rapid identification of at least <em>H. influenzae</em> type b could render treatment in some cases more effective by early application of a suitable antibiotic.</p></div><div><p>Sieben Methoden für die Serotypisierung von <em>Haemophilus influenzae</em> wurden erprobt. Beim Vergleich von Objektträgeragglutination, Staphylokokken-Koagglutination, Latexagglutination, Gegenstromimmunelektrophorese, Immunfluoreszenz, Kapselquellung und Kultivierung auf Antiserumagar erwies sich der kommerzielle Koagglutinationstest als am zuverlässigsten, schnellsten und am einfachsten durchzuführen. Um alle sechs Serotypen zu identifizieren, war bei diesem Koagglutinationstest eine Kombination mit der Objektträgeragglutination notwendig. Bei nahezu allen Methoden wurden beste Ergebnisse bei Verwendung von 6 h lang bei 37°C bebrüteten Kulturen erzielt. Da unbekapselte Stämme häufig unspezifisch agglutinierten, war eine Vorauswahl wahrscheinlich typisierbarer Stämme nützlich. Differenzierung anhand von Koloniemorphologie und -Opaleszenz wurde erleichtert durch Kultivierung auf Brain Heart Infusion (BHI) Chocolate Agar und Wuchsfak-tortestung auf klarem BHI-Agar mit Teststreifen für die Wuchsstoffe. Trübes Wachstum in Bouillon war ein Hinweis auf Bekapselung. Die Nigrosinfärbung, eine negative Kapseldarstellung, erwies sich als nützlich, wenn keine spezifischen Antiseren zur Verfügung standen.</p><p>Von 252 klinischen Isolaten von <em>H. influen","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 83-97"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80144-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of mycoplasma infections in cell cultures and a comparison of detection methods","authors":"Göran Bölske","doi":"10.1016/S0176-6724(88)80176-7","DOIUrl":"10.1016/S0176-6724(88)80176-7","url":null,"abstract":"<div><p>A total of 1424 cell cultures was assayed for mycoplasmas by microbiological culture and fluorescent DNA staining. Of these cultures, 412 (29%) were infected with mycoplasmas. The most frequently occurring mycoplasma species were <em>Mycoplasma orale</em> (34%), <em>M. hyorhinis</em> (26%), <em>M. arginini</em> (21%), <em>M. fermentans</em> (13%) and <em>Acholeplasma laidlawii</em> (5%). A few isolates each of <em>M. hominis, M. pulmonis</em> and <em>M. bovis</em> were also detected. When detection methods were compared, microbiological culture produced false-negative results for 0.7% (3 of 412) of the infected cell cultures. DNA staining performed directly on the cells was falsely negative in 2.4% (5/207) of the mycoplasma-infected cultures that were compared, DNA staining performed on indicator cells was falsely negative in 3.1% (7/226). False positives appeared in direct DNA-staining in 1.8% (7/386) of the mycoplasma-free cultures and with DNA staining on indicator cells in 0.5% (3/620). For 11% of the cell cultures, the reading of the DNA staining was ambiguous. With DNA staining on indicator cells, 10% of the test results were ambiguous, but by further passage and staining on new indicator cells it was possible to get a definite diagnosis.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 331-340"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80176-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14275974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Localization and characterization of fibronectin-binding to group A streptococci an electron microscopic study using protein-gold-complexes","authors":"Barbara Wagner ,&nbsp;Karl-Hermann Schmidt ,&nbsp;Manfred Wagner ,&nbsp;Torkel Wadström","doi":"10.1016/S0176-6724(88)80070-1","DOIUrl":"10.1016/S0176-6724(88)80070-1","url":null,"abstract":"<div><p>The location and nature of the binding sites for fibronectin (Fn) and its N-terminal 29 K fragment (FnF) on group A streptococci were studied by electron microscopy using these proteins labelled with colloidal gold. The investigated strains exhibited a different labelling intensity as well as a different labelling pattern varying from a strong regular distribution to a weak focal binding. Binding of Fn and FnF was inhibited by itself as well as by lipoteichoic acid (LTA), anti-LTA and concanavalin A. Simultaneous labelling of the bacteria with marker complexes of FnF, human serum albumin and fibrinogen revealed separate receptor sites for each protein. Our results confirmed LTA to be mainly responsible for the binding of Fn on group A streptococci.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 479-491"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80070-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14198103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Side Effects of Antibiotics on Immune Response Parameters and their Possible Implications in Antimicrobial Chemotherapy","authors":"G. Gillissen","doi":"10.1016/S0176-6724(88)80154-8","DOIUrl":"10.1016/S0176-6724(88)80154-8","url":null,"abstract":"<div><p>Antibiotics may influence immune response by quite different ways. By screening the multitude of publications on this subject, the aim of this overview was to arrive at a basic generalizing statement on the relationship between chemical structure or mode of action of antibiotics and the effect on immune response and to get an indication on whether certain in vitro and/or ex vivo parameters could represent comparable effects under clinical conditions. — The influence of antibiotics on immune response may arise by direct effects on immunocompetent cells, i.e. in the absence of microorganisms, or indirectly by changes in structure or metabolic products of germs induced by subminimal inhibitory concentrations (subMIC's). In the former case, stimulatory and inhibitory effects have been observed on phagocytosis and intracellular killing activity, on antibody production including IgE, on different parameters of cellular immunity (e.g. foodpad swelling reaction, MIF-production, mitogen/antigen induced lymphocyte proliferation and delayed type hypersensitivity skin reaction), on mediator production as interleukins or prostaglandins and the expression of corresponding receptors on immunocompetent cells as well as on the course of experimental infections with primary resistant microorganisms. — Indirect effects are related to the influence of subMIC's of antibiotics on the morphology and structure of microorganisms, on their antigenicity/immunogenicity or on their serosensitivity and enzyme and toxin production. — This overview shows that — according to the actual knowledge — antibiotics may exhibit immunological side effects which, however, can not strictly be attributed to certain chemical structures or to a certain mode of action. — It has to be considered that a literary study comparing the results of different authors is rendered difficult by the often non-homogeneity of experimental procedures and the fact that little is known yet about immunological side effects of antibiotics in man, i.e. under clinical conditions.</p></div><div><p>Antibiotika können die Immunantwort auf ganz verschiedene Weise beeinflussen. Es wurde versucht, die Vielzahl von Untersuchungen zu dieser Fragestellung zu sichten mit dem Ziel zu einer grundsätzlichen, verallgemeinernden Aussage zu gelangen über eine Beziehung zwischen chemischer Struktur oder Wirkungsmechanismus von Antibiotika und dem Effekt auf die Immunabwehr, sowie zu der Frage, welche der in vitro oder ex vivo nachgewiesenen Wirkungen einen Effekt unter klinischen Bedingungen repräsentieren können. — Die Beeinflussung der Immunantwort durch Antibiotika kann zustande kommen durch eiaen unmittelbaren Effekt auf immunkompetente Zellen, also in Abwesenheit von Mikroorganismen, oder indirekt über einen Einfluß von subminimalen Hemmkonzentrationen (subMHK) auf Struktur und Stoffwechselfunktion von Keimen. Im ersteren Fall wurden stimulierende oder hemmende Effekte beobachtet auf Phagozytose und die intrazelluläre","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 171-199"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80154-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14198107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Staphylococcus epidermidis on Cellular Immunity to Infection with Listeria monocytogenes","authors":"Stefan Ehlers ,&nbsp;Arne C. Rodloff ,&nbsp;Helmut Hahn","doi":"10.1016/S0176-6724(88)80155-X","DOIUrl":"10.1016/S0176-6724(88)80155-X","url":null,"abstract":"<div><p>With the present study, the effects of intravenous applications of <em>Staphylococcus epidermidis</em> (SE) on the course of experimental infections of mice with <em>Listeria monocytogenes</em> were evaluated. SE treatment 24 h prior to <em>Listeria</em> infection led to a reduced growth of <em>Listeria</em> organisms in both livers and spleens and to an increased resistance of infected animals against a lethal <em>Listeria</em> challenge. SE treatment 24 h after <em>Listeria</em> infection resulted in an enhanced growth of and retarded elimination of <em>Listeria</em> organisms from animal organs as well as in a reduction of delayed-type hypersensitivity to soluble <em>Listeria</em> antigen. Adoptive immunotherapy accomplished by transferring immune peritoneal exudate T-lymphocyte-enriched cells (PETLEs) to <em>Listeria</em>-infected recipients 24 h before SE treatment did not prevent the delay in clearance of <em>Listeria</em> organisms. When <em>Listeria</em>-infected recipients compromised in their immune response by SE treatment were infused with immune PETLEs either immediately or 24 h after the application of SE, the immunosuppression induced by SE proved to be reversible. It is concluded that, in analogy to other bacterial immunomodulators, <em>Staphylococcus epidermidis</em> is able to either nonspecifically activate macrophages or interfere with T-lymphocyte functions.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 200-212"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80155-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a Dot Blot Hybridization Assay for the Diagnosis of CMV Infection or Reactivation","authors":"Elisabeth Stöckl,&nbsp;Therese Popow,&nbsp;Franz Xaver Heinz ,&nbsp;Christian Kunz","doi":"10.1016/S0176-6724(88)80165-2","DOIUrl":"10.1016/S0176-6724(88)80165-2","url":null,"abstract":"<div><p>Dot blot hybridization was performed for the detection of cytomegalovirus (CMV) genomes in urine samples. This assay was applied to the diagnosis of CMV infection in transplant patients, who were tested continuously after transplantation and the results were compared to the detection of early antigen (EA) in fibroblasts inoculated with urine specimens as well as to serological methods.</p><p>It turned out that discrepancies between EA-detection and dot blot hybridization are partially caused by the different appearance and disappearance of the two parameters at different time points. In most cases the dot blot hybridization assay proved to be an earlier marker than EA-detection in the course of infection.</p><p>In several patients, however, hybridization showed positive signals although there was no sign for a productive CMV infection.</p></div><div><p>Dot Blot Hybridisierung wurde zum Nachweis von Zytomegalievirus-(CMV)-Genomen in Harnproben von Transplantationspatienten angewandt. Die Patienten wurden in regelmäßigen Intervallen vom Zeitpunkt der Transplantation an getestet, und die Ergebnisse wurden mit serologischen Daten und dem CMV-Frühantigen-(FA)-Nachweis in den mit Harnproben inokulierten Fibroblasten verglichen.</p><p>Unterschiede zwischen FA-Nachweis und Dot Blot Hybridisierung waren offensichtlich größtenteils dadurch bedingt, daß beide Tests in verschiedenen Stadien des Krankheitsverlaufs positive Resultate lieferten. In den meisten Fällen gab die Dot Blot Hybridisierung früher positive Resultate als der FA-Nachweis. Allerdings wurden auch bei einigen Patienten, die sicher nicht produktiv infiziert waren, ebenfalls CMV-Genome gefunden.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 288-294"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80165-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13989389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Granulocyte Activating Factor Released from Propionibacterium acnes. A Possible Mediator of Inflammation in Acne vulgaris","authors":"G. Pulverer ,&nbsp;W. Roszkowski ,&nbsp;H.J. Beuth ,&nbsp;H.L. Ko ,&nbsp;P. Quie","doi":"10.1016/S0176-6724(88)80160-3","DOIUrl":"10.1016/S0176-6724(88)80160-3","url":null,"abstract":"<div><p>Incubation of <em>Propionibacterium acnes</em> but not of <em>Propionibacterium granulosum</em> or <em>Propionibacterium avidum</em> (for 30 min at 37°C in physiological saline) released a soluble factor that produced enhanced chemiluminescence response of human granulocytes as well as increased chemotactic motility of these cells. Sephadex G-25 filtration of the granulocyte activating factor (GAF) revealed its low molecular weight and apparent peptide character. Thus, GAF may be a stimulus for inflammation in acne vulgaris since low molecular weight chemotactic factors can be expected to penetrate follicular walls.</p></div><div><p>Inkubation von <em>Propionibacterium acnes</em> in physiologischer Pufferlösung (30 Min., 37 °C) bewirkt eine massive Freisetzung eines löslichen, Granulozyten aktivierenden Faktors (GAF); <em>Propionibacterium avidum</em> und <em>Propionibacterium granulosum</em> besaßen diese Fähigkeit hingegen nicht. Im Chemilumineszenz- und Chemotaxis-Test mit menschlichen Granulozyten konnte eine stark gesteigerte Aktivität der Zellen nach Inkubation mit GAF nachgewiesen werden. Sephadex G-25 Filtration des GAF offenbarte ein niedriges Molekulargewicht. Es wird vermutet, daß es sich um ein Peptid handelt. GAF ist offenbar einer der Stimuli für Entzündungsreaktionen in Akne vulgaris. Aufgrund des niedrigen Molekulargewichtes ist anzunehmen, daß GAF die Follikelwand penetrieren und Entzündungserscheinungen initiieren und aufrechterhalten kann.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 246-251"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80160-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14110075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactogenicity and immunogenicity of a new recombinant yeast-derived hepatitis B vaccine","authors":"Norbert Scheiermann ,&nbsp;Karl Michael Gesemann ,&nbsp;Dietrich Paar ,&nbsp;Claus Maurer","doi":"10.1016/S0176-6724(88)80184-6","DOIUrl":"10.1016/S0176-6724(88)80184-6","url":null,"abstract":"<div><p>Under randomized double-blind conditions, 220 medical students were vaccinated with either a 2.5, 5,10, or 20 μg dose of a recombinant yeast-derived hepatitis B or a 20 μg dose of a plasma-derived vaccine. Vaccines were administered at months 0, 1, and 2. After 11 months, all vaccinees received a 20 μg booster dose of the recombinant vaccine.</p><p>There were no significant differences in adverse reactions between the study groups. Induction of IgE antibodies to yeast was not observed. One month after the third vaccination, seroconversion rates reached 100% in all vaccinees. Mean anti-HBs levels varied between 150 and 1470 IU/l after 3 vaccinations, with the lowest dose resulting in the lowest titres. Following the booster vaccination, dose-dependent effects were no longer observed. Anti-HBs concentrations were reanalyzed 29 and 36 months after the study had started. The data indicate that the recombinant hepatitis B vaccine is safe and immunogenic for use in man and comparable to the plasma-derived vaccine in terms of safety and efficacy.</p></div><div><p>In einer randomisierten Doppelblindstudie wurden 220 junge, gesunde Erwachsene mit unterschiedlichen Dosen (2,5; 5; 10; 20 μg) einer neuen, rekombinanten Hepatitis-B-Vakzine auf Hefezellbasis geimpft. In einer Kontrollgruppe erfolgte die Impfung mit der 20 μg Dosis eines Plasmaderivatimpfstoffs. Die Impfungen erfolgten zu Beginn der Studie, nach einem und nach zwei Monaten (0–1–2). Elf Monate nach der ersten Impfung erhielten alle Impflinge die 20 μg Dosis der rekombinanten Vakzine.</p><p>Die Nebenwirkungen waren in den 5 Gruppen ähnlich gering. IgE Antikörper gegen Hefematerial wurden nicht induziert. Ein Monat nach der dritten Impfung wurde zu 100% Serokonversion erzielt. Die mittleren anti-HBs-Antikörperspiegel lagen nach drei Impfungen zwischen 150 und 1470 IU/l, wobei die niedrigste Dosis die niedrigsten Werte ergab. Nach der Booster-Impfung wurden keine dosisabhängigen Wirkungen mehr gesehen. In den 5 Impfgruppen lagen zum Monat 12 die mittleren anti-HBs-Werte zwischen 9230 und 51404 IU/l. Kontrolluntersuchungen erfolgten 29 und 36 Monate nach Studienbeginn. Die Studie hat gezeigt, daß die rekombinante Hepatitis B-Vakzine sicher und immunogen beim Menschen ist und vergleichbare Wirkungen wie der Plasmaderivatimpfstoff zeigt.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 411-421"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80184-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14110753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth conditions for the expression of fibronectin and collagen binding to Salmonella","authors":"Enrique A. González ,&nbsp;Suraj B. Baloda ,&nbsp;Jorge Blanco ,&nbsp;Torkel Wadström","doi":"10.1016/S0176-6724(88)80065-8","DOIUrl":"10.1016/S0176-6724(88)80065-8","url":null,"abstract":"<div><p>Binding of ¹²⁵I-fibronectin, its ¹²⁵I-labelled 29-kDa aminoterminal fragment, and ¹²⁵I-collagen to cells of 13 Salmonella strains grown in broth and agar media at three different temperatures was studied. Of the 13 strains, 7 had only smooth colony morphologies while three strains were pairs of both smooth strains and their corresponding rough variants. The three rough variants showed higher binding to fibronectin, it's 29-kDa fragment and to collagen, than the corresponding smooth forms. However, the percentage of ¹²⁵I-protein bound was greatly influenced by the growth conditions. In these three pairs of strains, there was a direct correlation between cell-surface hydrophobicity and the binding activity, but this correlation was not observed in the remaining strains. Thus, some of the strains showed high cell-surface hydrophobicity but low binding activity under optimal growth conditions. The highest binding rates of fibronectin and of it's 29-kDA fragment were obtained with bacteria grown on colonisation factor antigen (CFA) agar at 33 °C, while the binding to collagen was slighly higher when bacteria were cultured on tryptic soy agar.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 437-446"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80065-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14352513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}