Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

{"title":"Effects of Staphylococcus epidermidis on Cellular Immunity to Infection with Listeria monocytogenes","authors":"Stefan Ehlers ,&nbsp;Arne C. Rodloff ,&nbsp;Helmut Hahn","doi":"10.1016/S0176-6724(88)80155-X","DOIUrl":"10.1016/S0176-6724(88)80155-X","url":null,"abstract":"<div><p>With the present study, the effects of intravenous applications of <em>Staphylococcus epidermidis</em> (SE) on the course of experimental infections of mice with <em>Listeria monocytogenes</em> were evaluated. SE treatment 24 h prior to <em>Listeria</em> infection led to a reduced growth of <em>Listeria</em> organisms in both livers and spleens and to an increased resistance of infected animals against a lethal <em>Listeria</em> challenge. SE treatment 24 h after <em>Listeria</em> infection resulted in an enhanced growth of and retarded elimination of <em>Listeria</em> organisms from animal organs as well as in a reduction of delayed-type hypersensitivity to soluble <em>Listeria</em> antigen. Adoptive immunotherapy accomplished by transferring immune peritoneal exudate T-lymphocyte-enriched cells (PETLEs) to <em>Listeria</em>-infected recipients 24 h before SE treatment did not prevent the delay in clearance of <em>Listeria</em> organisms. When <em>Listeria</em>-infected recipients compromised in their immune response by SE treatment were infused with immune PETLEs either immediately or 24 h after the application of SE, the immunosuppression induced by SE proved to be reversible. It is concluded that, in analogy to other bacterial immunomodulators, <em>Staphylococcus epidermidis</em> is able to either nonspecifically activate macrophages or interfere with T-lymphocyte functions.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 200-212"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80155-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lektin-typisierung als leistungsfähiges epidemiologisches markersystem für Neisseria gonorrhoeae-infektionen","authors":"Hans Christian Korting ,&nbsp;Dietrich Abeck","doi":"10.1016/S0176-6724(88)80073-7","DOIUrl":"10.1016/S0176-6724(88)80073-7","url":null,"abstract":"<div><p>A total of 102 <em>Neisseria gonorrhoeae</em> isolates from Munich with known nutritional requirements were examined for lectin agglutination patterns using Taxonolectin panels containing 14 different plant originated lectins with known specificity. 29 different lectin agglutination patterns were found (in comparison auxotyping showed 17 different groups). All strains reacted with Concanavalin A and <em>Trichosantbes kinlowii</em> and did not show positive reactions with <em>Limax flavus</em> and <em>Ulex europaeus</em> I. 49 Isolates (48%) had lectin agglutination patterns associated with only four lectin groups (in comparison the four major auxo typing groups comprised 58 (57%) of the tested isolates). A correlation between auxotype and lectin agglutination pattern could not be demonstrated. Reproducibility of lectin agglutination patterns was excellent.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 506-512"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80073-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127387736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection and Immunity to Vibrio cholerae, Salmonella typhimurium and Escherichia coli in a Rabbit Model","authors":"Piet A.M. Guinée ,&nbsp;Wim H. Jansen,&nbsp;Sjoerd G.T. Rijpkema","doi":"10.1016/S0176-6724(88)80162-7","DOIUrl":"10.1016/S0176-6724(88)80162-7","url":null,"abstract":"<div><p>Cholera disease can be induced in the rabbit by duodenal inoculation (DI) of <em>Vibrio cholerae</em> organisms after ligation of the cecum (C) (DIC model). When ligation of the cecum is omitted, no disease symptoms develop. In contrast, the animals are primed which becomes apparent as vibriocidal protection upon challenge with <em>V. cholerae</em> in the DIC model. This protection coincides with high anti-O antigen IgA levels in the bile. The O antigen was shown to be the protective antigen and it must be presented by live organisms. A non-enterotoxigenic mutant of <em>V. cholerae</em> induced protective immunity in the rabbit but was reported to cause mild diarrhea in human volunteers. Looking for alternatives, we applied cholera toxin, known as a mucosal adjuvant, together with killed <em>V. cholerae</em> cells to rabbits. Unfortunately, the minimum adjuvant dose was equal to the minimum toxic dose. A Salmonella typhimurium strain expressing also the <em>V. cholerae</em> O antigen induced systemic rather than local immunity which was not protective. Several Escherichia coli strains were able to elicit a local immune response, but the animal to animal differences were considerable. Therefore, <em>V. cholerae</em> itself was thought to be the most appropriate carrier organism. Some non-enterotoxigenic and auxotrophic mutants of <em>V. cholerae</em> were able to prime and did not show any undesired side-effects in the DIC model. Therefore, further attenuation of non-toxigenic <em>V. cholerae</em> strains by means of stable deletions in nutritional genes seems to be the most promising way to obtain acceptable vaccine candidates.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 260-269"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80162-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13610551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leptospiral lipopolysaccharide presence in the outer envelope: Electrophoretic evidence and immunological specificity","authors":"Marina Cinco ,&nbsp;Elena Banfi ,&nbsp;Enrico Panfili","doi":"10.1016/S0176-6724(88)80171-8","DOIUrl":"10.1016/S0176-6724(88)80171-8","url":null,"abstract":"<div><p>Purified preparations of lipopolysaccharides (LPS) extracted from two different strains of <em>Leptospira interrogans</em> have been electrophoretically analyzed in order to determine their location at the level of outer envelope (OE). Evidence has been collected for the presence of some LPS fractions in the OE, suggesting that a part of this molecule is embedded in the membrane structure. The serological specificity of the LPS has been in addition tested by means of monoclonal antiserovar antibodies (Moabs); the results indicated that the LPS structure is endowed of the immunodeterminants of the serovar. The remarkable relevance of this finding for the <em>Leptospira</em> taxonomy is discussed.</p></div><div><p>Die aus <em>Leptospira interrogans</em> Serovar <em>copenhageni</em> bzw. <em>hardjo</em> isolierten und gereinigten Lipolysaccharide (LPS) wurden elektrophoretisch untersucht, um damit ihre Lokalisation in der äußeren Hülle zu bestimmen. Der Nachweis von LPS-Fraktionen in der äußeren Hülle legt die Annahme nahe, das ein Teil des Moleküls in die Membran-Struktur eingebaut ist. Mittels monoklonaler, serovarspezifischer Antikörper wurde gezeigt, daß LPS zu der immundominanten Struktur der Serovare beiträgt.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 277-283"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80171-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13609796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral DNA from Cells Infected with Porcine Circovirus","authors":"Ilse Tischer ,&nbsp;Hans-Jörg Buhk","doi":"10.1016/S0176-6724(88)80164-0","DOIUrl":"10.1016/S0176-6724(88)80164-0","url":null,"abstract":"<div><p>It has been demonstrated earlier that the genome of the porcine circovirus (PCV) isolated from virions is a circular single-stranded DNA (ssDNA). This report describes the isolation and characterization of PCV-specific DNA from infected cell cultures. Two types of doublestranded DNA (dsDNA) were observed which behaved like supercoiled and relaxed circular molecules, respectively. Both types of dsDNA displayed infectivity in transfection experiments and are regarded as replicative forms (RF) of the viral genome. In addition to complete single-stranded viral genomes and RF DNA molecules, PCV-specific subgenomic fractions of DNA molecules with a sedimentation coefficient of about 5 S were isolated from infected tissue culture cells. This 5 S DNA was also isolated from purified virions. It is single-stranded and represents a defined region of the viral genome.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 280-287"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80164-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13989388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas aeruginosa Lectins as a Model for Lectin Production, Properties, Applications and Functions","authors":"Nechama Gilboa-Garber","doi":"10.1016/S0176-6724(88)80135-4","DOIUrl":"10.1016/S0176-6724(88)80135-4","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is one of the most troublesome human pathogens in the antibiotic consuming era. It produces lectins and lectinoid adhesins as secondary metabolites. The production of these compounds is genetically determined and is highly sensitive to changing environmental conditions. These dictate the type of the lectin produced [“type” variation], the lectin level [“on-off” variation], and its localization [“in-out” variation]. PA-I [galactophilic] and PA-II [fucose and mannose-binding] <em>P. aeruginosa</em> lectins are sensitive to EDTA and exhibit biophysical properties, resembling those of classical plant lectins. They exert similar in vitro biological effects and have an equal applicative potential. Lectin deficient strains and mutants of <em>P. aeruginosa</em> may be used for studies on lectin role in “conditioning” the bacterium lytic and toxic activities in its attacks on cells or macromolecules. The <em>Pseudomonas</em> lectins cofunction with lytic and toxic activities: We suggest that they serve the homing and “condition” the lytic enzyme optimal activity on cellular and macromolecular targets. Namely their role resembles that of “positioning sites” of lytic enzymes and “receptor-binding” domains of powerful microbial, plant and animal toxic or lytic systems [including immunoglobulins, which “condition” the lytic activities of complement and phagocytes], as well as certain hormones, which condition limited key lytic activities, and thereby trigger a cascade of metabolic reactions.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 3-15"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80135-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of chlamydia trachomatis by isolation in cell culture and enzyme amplified immunoassay","authors":"T.F. Schwarz ,&nbsp;G. Zoulek,&nbsp;M. Roggendorf","doi":"10.1016/S0176-6724(88)80177-9","DOIUrl":"10.1016/S0176-6724(88)80177-9","url":null,"abstract":"<div><p>188 (139 ♀ ; 49 ♂) genital swabs from patients with urogenital complaints (&gt; 6 weeks), 69 (33 ♀ ; 36 ♂) conjunctival swabs from patients with chronic conjunctivitis and 14 swabs from newborns with acute conjunctivitis were tested for the presence of <em>Chlamydia trachomatis</em> (Ct) by inoculation in cell culture and visualisation by indirect immunfluorescence (IFT) with a monoclonal antibody and enzyme immunoassay (ELISA). Of the 271 specimens 20 (7.4%) were Ct positive by cell culture (IFT) and 18 (6.6%) were positive by ELISA. In 184 (97.9%) urogenital specimens results between cell culture (IFT) and ELISA agreed. With ELISA 4 further genital specimens were found to be Ct positive. In 59 (85.5%) conjunctival swabs of patients with chronic conjunctivitis results between cell culture (IFT) and ELISA agreed. By inoculation of cell culture (IFT) 6 (8.7%) more Ct positive specimens could be identified which were negative in ELISA. There was complete agreement between positive and negative Ct detection with cell culture (IFT) and ELISA in the cases of acute conjunctivitis.</p></div><div><p>188 (139 ♀ ; 49 ♂) Genitalabstriche von Patienten mit urogenitalen Beschwerden (≥ 6 Wochen), 69 (33 ♀ ; 36 ♂) Konjunktivalabstriche von Patienten mit einer chronischen Konjunktivitis und 14 Konjunktivalabstriche von Neugeborenen mit einer akuten Konjunktivitis wurden auf <em>Chlamydia trachomatis</em> (Ct) untersucht. Der Ct-Nachweis erfolgte durch Inokulation in Zellkultur und anschließender indirekter Immunfluoreszenz (IFT) oder mit einem Enzymimmunoassay (ELISA) zum direkten Erregernachweis. Ct wurde bei 20 (7,4%) von insgesamt 271 Abstrichen mittels Zellkultur (IFT) und bei 18 (6,6%) mittels ELISA nachgewiesen. Bei 184 (97,9%) Urogenitalabstrichen konnte eine Übereinstimmung der positiven und negativen Ergebnisse zwischen Zellkultur und ELISA erzielt werden. Mittels ELISA wurden vier weitere Abstriche positiv bewertet. Die Ergebnisse der vergleichenden Untersuchung der Konjunktivalabstriche der Patienten mit chronischer Konjunktivitis stimmten bei 59 Proben (85,5%) überein. Durch Zellkulturnachweis (IFT) wurden 6 (8,7%) weitere Ct positive Abstriche, die im ELISA negativ waren, entdeckt. Eine absolute Übereinstimmung zwischen positiven und negativen Ergebnissen ergab die Untersuchung von Abstrichen von Neugeborenen mit akuter Konjunktivitis.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 3","pages":"Pages 341-345"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80177-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14197165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Shwartzman Phenomenon on the Pathogenesis of Experimental Acute Pyelonephritis in Rat","authors":"Rudolf Sonak ,&nbsp;Michael Balduf","doi":"10.1016/S0176-6724(88)80149-4","DOIUrl":"10.1016/S0176-6724(88)80149-4","url":null,"abstract":"<div><p>It has been shown that the mere presence of gram-negative bacteria within the kidney parenchyma does not lead to kidney damage. Various accessory manipulations modifying the resistance of the urinary tract should be used to induce kidney lesions. We present the results of our experiments with sterile bacterial filtrate applied accordingly to the technique of Shwartzman phenomenon. This way we produced experimental acute pyelonephritis in rats by an ascending route of infection. A significant pathogenic activity of sterile bacterial filtrate in comparison with saline was shown producing clinically relevant infections of the urinary tract in rats infected by concentrations of <em>E. coli</em> suspensions of 10⁴ and 10⁵ cfu/ml.</p></div><div><p>Die gram-negativen Bakterien allein rufen in den Nieren von Versuchstieren kaum einen entzündlichen Prozeß hervor. Verschiedene zusätzliche Manipulationen, durch welche die Resistenz des Harnwegsystems modifiziert wird, sind hierzu notwendig. In dieser Arbeil werden die Ergebnisse unserer Versuche mit sterilem bakteriellem Filtrat dargelegt. Nach der Shwartzman-Phänomen-Technik wurde das Filtrat zur Einleitung einer experimentellen Ratten-Pyelonephritis, hervorgerufen durch aszendierende bakterielle Infektion, verwendet Eine signifikante pathogenetische Wirkung des bakteriellen Filtrates gegenüber den Kontrollen mit physiologischer NaCl-Lösung konnte gezeigt werden (p &lt; 0,01). Die auf diese Weise erzeugten klinisch-relevanten Harnwegsinfektionen wurden bei den Ratten durch die Suspensionen von <em>E. coli</em> in den Konzentrationen von 10⁴ und 10⁵ cfu/ml hervorgerufen.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 131-137"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80149-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14198105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stomatococcus mucilaginosus as an Agent of CAPD Peritonitis","authors":"Helen Lanzendörfer ,&nbsp;Karel Zaruba ,&nbsp;Alexander Von Graevenitz","doi":"10.1016/S0176-6724(88)80170-6","DOIUrl":"10.1016/S0176-6724(88)80170-6","url":null,"abstract":"<div><p>We report a case of bacterial CAPD peritonitis in a 57 year old immunosuppressed woman with renal insufficiency caused by a rare species of the <em>Micrococcaceae, Stomatococcus mucilaginosus</em>.</p><p>This uncommon case shows that the presence of gram-positive cocci in pairs, tetrads and clusters forming whitish-gummy colonies should remind us of the possibility of <em>Stomatococcus mucilaginosus</em>.</p></div><div><p>Beschrieben wird ein Fall einer bakteriellen CAPD-Peritonitis, der bei einer 56jährigen, immunsupprimierten Patientin mit terminaler Niereninsuffizienz auftrat und durch einen seltenen Vertreter der <em>Micrococcaceae (Stomatococcus mucilaginosus)</em> verursacht wurde.</p><p>Dieser mikrobiologisch außergewöhnliche Fall zeigt, daß bei grampositiven Kokken, die in Haufen, Tetraden und Paaren angeordnet sind und weiße, gummiartige Kolonien bilden, <em>Stomatococcus mucilaginosus</em> in Betracht gezogen werden sollte.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 1","pages":"Pages 326-328"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80170-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14350449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"I. Verzeichnis der in Band 269 enthaltenen Arbeiten","authors":"","doi":"10.1016/S0176-6724(88)80075-0","DOIUrl":"https://doi.org/10.1016/S0176-6724(88)80075-0","url":null,"abstract":"","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"269 4","pages":"Pages 566-582"},"PeriodicalIF":0.0,"publicationDate":"1988-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(88)80075-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137162369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}