Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology最新文献

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Genome type analysis of certain adenovirus serotypes of subgenus D D亚属腺病毒血清型基因组型分析
Th. Adrian , R. Wigand
{"title":"Genome type analysis of certain adenovirus serotypes of subgenus D","authors":"Th. Adrian ,&nbsp;R. Wigand","doi":"10.1016/S0176-6724(89)80024-0","DOIUrl":"10.1016/S0176-6724(89)80024-0","url":null,"abstract":"<div><p>The DNA of 19 wild strains of adenovirus types 10 (AV10), 13, 19, 24, and 27 was analysed by five restriction endonucleases (BamHI, BglII, BstEII, HindIII, and SmaI) for their genetic variability and compared wih their respective prototypes. Six “AV19a” strains were identical among each other, even with five additional enzymes, but widely different from the prototype. Strains of AV10, 24, and 27 were identical or only slightly different from the respective prototypes. In contrast, three AV13 strains differed in all enzyme patterns from those of the prototype and from one another.</p></div><div><p>Die DNA von 19 Wildstämmen der Adenovirustypen 10(AV10), 13, 19, 24 und 27 wurde mit 5 Restriktionsendonukleasen (BamHI, BglII, BstEII, Hindlll und SmaI) untersucht und mit derjenigen der entsprechenden Prototypen verglichen, um die genetische Variabilität dieser Typen zu beurteilen. Sechs “AV19a”-Stämme waren untereinander einheitlich, und dies sogar mit 5 weiteren Enzymen, aber stark abweichend vom Prototyp. Stämme von AV10, 24 und 27 waren entweder gleich oder nur geringfügig verschieden von den zugehörigen Prototypen. Dagegen unterscheiden sich drei AV13-Stämme in sämtlichen Enzymen vom Prototyp und untereinander.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 527-533"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80024-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13684314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Immunodiagnosis of human Anisakiasis by use of larval excretory-secretory antigen 利用幼虫排泄-分泌抗原对人异丝虫病进行免疫诊断
Ulrich Poggensee , Gabriele Schommer, Rolf Jansen-Rosseck, Hermann Feldmeier
{"title":"Immunodiagnosis of human Anisakiasis by use of larval excretory-secretory antigen","authors":"Ulrich Poggensee ,&nbsp;Gabriele Schommer,&nbsp;Rolf Jansen-Rosseck,&nbsp;Hermann Feldmeier","doi":"10.1016/S0176-6724(89)80021-5","DOIUrl":"10.1016/S0176-6724(89)80021-5","url":null,"abstract":"<div><p>L<sub>3</sub> larvae of <em>Anisakis simplex</em>, isolated from freshly caught herrings were cultured in Medium 199 at different temperatures to obtain excretory-secretory (ES) antigen. Larvae have been cultured in vitro for a period of 11 months. A crude extract of <em>A. simplex</em> larvae was compared with ES antigen obtained under 4 different culture conditions for the determination of IgG and IgE antibodies by ELISA and a modified RAST, respectively. 1 serum from a patient with histopathologically proved anisakiasis and 4 samples from persons with presumptive clinical disease were positive in the ELISA. Two sera were positive in the RAST. A combination of both tests is suggested for the serodiagnosis of human anisakiasis.</p></div><div><p>L<sub>3</sub> Larven von <em>Anisakis simplex</em> aus frisch gefangenen Heringen wurden unter vier verschiedenen Temperaturbedingungen in Medium 199 zur Gewinnung von exkretorisch-sekretorischem (ES-) Antigen in Kultur gehalten. Seit 11 Monaten werden die Larven in vitro kultiviert. Rohantigen von <em>A. simplex</em> und die unterschiedlichen ES-Antigenpräparationen wurden im ELISA und einem modifizierten RAST zur Bestimmung von IgG und IgE eingesetzt und miteinander verglichen. Das Serum eines Patienten, der an einer histopathologisch erwiesenen Anisakiasis litt, und Seren von 4 Patienten, bei denen der klinische Verdacht einer Anisakiasis bestand, waren im ELISA positiv. IgE Antikörper wurden in zwei Fällen nachgewiesen. Eine Kombination beider Tests erscheint sinnvoll für die Diagnosestellung der Anisakisasis.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 503-510"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80021-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13851474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Detection and interspecies-transformation of a β-Lactamase-encoding plasmid from Pasteurella haemolytica 溶血巴氏杆菌β-内酰胺酶编码质粒的检测及种间转化
St. Schwarz , U. Spies , B. Reitz , H.-M. Seyfert , Ch. Lämmler , H. Blobel
{"title":"Detection and interspecies-transformation of a β-Lactamase-encoding plasmid from Pasteurella haemolytica","authors":"St. Schwarz ,&nbsp;U. Spies ,&nbsp;B. Reitz ,&nbsp;H.-M. Seyfert ,&nbsp;Ch. Lämmler ,&nbsp;H. Blobel","doi":"10.1016/S0176-6724(89)80017-3","DOIUrl":"10.1016/S0176-6724(89)80017-3","url":null,"abstract":"<div><p><em>Pasteurella haemolytica</em>-cultures, isolated from cattle with respiratory diseases, were investigated for their biotype, serotype, antimicrobial resistance and plasmid content. A plasmid encoding a β-lactamase could be demonstrated in 9 of 19 <em>Pasteurella haemolytica</em>-cultures. These 9 cultures, all belonging to biotype A and serotype 1, were resistant to ampicillin, carbenicillin, penicillin G and ticarcillin. The plasmid of the respective cultures proved to be identical upon Southern blot hybridization. It could be transformed into <em>Escherichia coli</em> 490 A where it expressed again a β-lactamase-activity.</p></div><div><p><em>Pasteurella haemolytica</em>-Kulturen von Rindern mit respiratorischen Krankheiten wurden hinsichtlich ihres Biotyps, Serotyps, ihrer Antibiotika-Resistenz und ihres Plasmidgehaltes untersucht.</p><p>Bei 9 von 19 <em>Pasteurella haemolytica</em>-Isohtm konnte ein Plasmid nachgewiesen werden, das für eine β-Lactamaseaktivität codiert. Diese 9 Kulturen, die alle zum Biotyp A und Serotyp 1 gehören, waren resistent gegenüber Ampicillin, Carbenicillin, Penicillin G und Ticarcillin. Durch Southern blot-Hybridisierung konnte gezeigt werden, daß die gefundenen Plasmide identisch sind. In <em>Escherichia coli</em> 490 A übertragen entfaltet dieses <em>Pasteurella haemolytica</em>-Plasmid ebenfalls eine β-Lactamaseaktivität.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 462-469"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80017-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13793522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Comparison of different serotests for specific toxoplasma IgM-antibodies (ISAGA, SPIHA, IFAT) and detection of circulating antigen in two cases of laboratory acquired toxoplasma infection 2例实验室获得性弓形虫感染不同血清igm抗体(ISAGA、SPIHA、IFAT)和循环抗原检测的比较
Kurt Hermentin , Andreas Hassl , Otto Picher , Horst Aspöck
{"title":"Comparison of different serotests for specific toxoplasma IgM-antibodies (ISAGA, SPIHA, IFAT) and detection of circulating antigen in two cases of laboratory acquired toxoplasma infection","authors":"Kurt Hermentin ,&nbsp;Andreas Hassl ,&nbsp;Otto Picher ,&nbsp;Horst Aspöck","doi":"10.1016/S0176-6724(89)80025-2","DOIUrl":"10.1016/S0176-6724(89)80025-2","url":null,"abstract":"<div><p>Two symptomatic <em>Toxoplasma</em> infections of laboratory personnel have been serologically followed up for 5.5 and 10 months, respectively. Results obtained by commonly used test systems (indirect fluorescent antibody tests for IgG and IgM antibodies, complement fixation test) were compared with those of two recently developed and improved tests for IgM detection (immunosorbent agglutination assay [ISAGA] and solid-phase indirect haemadsorption assay [SPIHA] as well as with those of a test designed for the detection of circulating antigen (cag-ELISA).</p></div><div><p>Zwei klinisch apparente Laborinfektionen mit <em>Toxoplasma gondii</em> wurden 5,5 bzw. 10 Monate lang serologisch verfolgt. Die in den herkömmlichen Testsystemen (Indirekter Immunfluoreszenztest für IgG-, IgM-Antikörper, Komplementbindungsreaktion) erbrachten Ergebnisse wurden mit jenen Ergebnissen verglichen, die in zwei kürzlich entwickelten und verbesserten Tests zur Bestimmung von IgM-Antikörpern (immunosorbent agglutination assay [ISAGA], solid-phase indirect haemadsorption assay [SPIHA]) erzielt wurden. Außerdem wurden die Ergebnisse eines Enzymimmuntests zum Nachweis von zirkulierendem Antigen (cag-ELISA) in die vergleichenden Untersuchungen einbezogen.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 534-541"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80025-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13793525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Inhaltsverzeichnis 目录。
{"title":"Inhaltsverzeichnis","authors":"","doi":"10.1016/S0176-6724(89)80026-4","DOIUrl":"https://doi.org/10.1016/S0176-6724(89)80026-4","url":null,"abstract":"","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 542-546"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80026-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137400904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolierung und identifizierung von Mykoplasmen und Chlamydien 菌斑和衣原体的分离和识别
Deutsche Gesellschaft für Hygiene und Mikrobiologie
{"title":"Isolierung und identifizierung von Mykoplasmen und Chlamydien","authors":"Deutsche Gesellschaft für Hygiene und Mikrobiologie","doi":"10.1016/S0176-6724(89)80018-5","DOIUrl":"10.1016/S0176-6724(89)80018-5","url":null,"abstract":"","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 470-486"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80018-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125179578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Monoclonal antibodies to protein I for serotyping of Neisseria gonorrhoeae: Correlation of serotype with bactericidal activity 用于淋病奈瑟菌血清分型的蛋白I单克隆抗体:血清型与杀菌活性的相关性
Peter K. Kohl , Duane A. Olsen , Thomas M. Buchanan
{"title":"Monoclonal antibodies to protein I for serotyping of Neisseria gonorrhoeae: Correlation of serotype with bactericidal activity","authors":"Peter K. Kohl ,&nbsp;Duane A. Olsen ,&nbsp;Thomas M. Buchanan","doi":"10.1016/S0176-6724(89)80023-9","DOIUrl":"10.1016/S0176-6724(89)80023-9","url":null,"abstract":"<div><p>Seven monoclonal antibodies have been used for the serotyping of one hundred <em>Neisseria gonorrhoeae</em> wild strains, randomly selected from nine U.S. cities, and seven serotype reference strains by the co-agglutination method. As determined by gel-immunoradioassay, the monoclonal antibodies recognized the protein I trimer of a single or a limited subset of serotype reference strains. All but three strains were typable by one or two of the antibodies. The most common serotypes were 1,3 (26%), 1 (20%), 5 (17%), 5,7 (11%) and 9 (10%). To correlate typing results with ability for killing of these antibodies, susceptibility of typed and non-typed strains to killing was studied. Susceptibility was significantly associated with typing by the serotype 7 (p = 0.011) and serotype 9 (p = 0.033) specific monoclonal antibodies. Reaction of antibodies recognizing epitopes on the protein IB molecule with a given strain predicted in an average of 43% of strains (49% of strains of serotype 5, 62% of serotype 7, 29% of serotype 8, and 33% of serotype 9) its susceptibility to killing by the typing antibodies. In contrast, only 15% of the strains (15% of strains of serotype 1 and 15% of serotype 3) were killed by their typing antibodies, recognizing epitopes on the protein IA molecule. These monoclonal antibodies might prove to be important for the isolation and structural characterization of epitopes responsible for susceptibility of the gonococcus to killing and thus for the development of a vaccine against invasive gonococcal disease.</p></div><div><p>Sieben monoklonale Antikörper wurden mit Hilfe der Co-Agglutinationsmethode zur Serotypisierung von einhundert <em>Neisseria gonorrhoeae</em> Wildstämmen, die willkürlich in neun amerikanischen Städten ausgewählt wurden, und sieben Serotyp-Referenzstämme verwendet. Die monoklonalen Antikörper erkannten, wie mit Hilfe des Gel-immunoradioassays bestimmt, das Protein I-Trimer eines einzelnen oder einer begrenzten Untereinheit von Serotyp-Referenstämmen. Alle außer drei Stämmen waren mit einem oder zwei Antikörpern typisierbar. Die häufigsten Serotypen waren 1,3 (26%), 1 (20%), 5 (17%), 5,7 (11%) und 9 (10%). Um die Typisierung mit der Fähigkeit dieser Antikörper zur Abtötung zu vergleichen, wurden typisierte und nicht typisierte Stämme in einem Bakterizidietest untersucht. Die Abtötbarkeit war signifikant assoziiert mit der Typisierung durch die Serotyp 7 (p = 0,011) und Serotyp 9 (p = 0,033) spezifischen monoklonalen Antikörper. Die Reaktion eines bestimmten Stammes mit Antikörpern, die Epitope auf dem Protein IB Molekül erkennen, sagte in durchschnittlich 43% der Fälle (49% der Serotyp 5-Stämme, 62% der Serotyp 7-Stämme, 29% der Serotyp 8-Stämme und 33% der Serotyp 9-Stämme) die Abtötbarkeit durch den typisierenden Antikörper voraus. Im Gegensatz dazu wurden nur 15% der Stämme abgetötet (15% der Serotyp 1-Stämme und 15% der Serotyp 3-Stämme), die mit monoklonalen Antikörpern reagieren, die Epitope auf de","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 517-526"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80023-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13642765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Rapid diagnosis of intestinal Bacteroides species by means of the immunofluorescence 免疫荧光法快速诊断肠道拟杆菌
Claus Höhne , Dieter Sandow, Barbara Wolters, Gertraude Hübner
{"title":"Rapid diagnosis of intestinal Bacteroides species by means of the immunofluorescence","authors":"Claus Höhne ,&nbsp;Dieter Sandow,&nbsp;Barbara Wolters,&nbsp;Gertraude Hübner","doi":"10.1016/S0176-6724(89)80020-3","DOIUrl":"10.1016/S0176-6724(89)80020-3","url":null,"abstract":"<div><p>Rapid methods serving the detection of intestinal <em>Bacteroides</em> species in clinical specimens are of great significance. For this purpose, an identification of the species is possible at present only by means of immunofluorescence (IF). Using preparations of antigens of different intestinal <em>Bacteroides</em> species (<em>B. fragilis, B. thetaiotaomicron, B. uniformis, B. ovatus</em>, and <em>B. distasonis</em>) as well as immune sera prepared against them, the presented results have shown the possibility of specific identification of the species involved by means of direct and indirect IF, respectively. However, it could be demonstrated that the direct IF results in a more brillant appearance of the bacteria than the indirect procedure. Convincing results could be obtained also in the investigation of clinical specimens by means of the direct IF using a pool of conjugated immunoglobulin fractions (sensitivity 88%, specificity 100%). Therefore, the direct method to detect intestinal <em>Bacteroides</em> species by IF should be preferred.</p></div><div><p>Für den Nachweis intestinaler <em>Bacteroides</em>-Arten in klinischen Untersuchungsproben kommt schnelldiagnostischen Methoden ein hoher Stellenwert zu. Diesbezüglich kann gegenwärtig eine Speziesidentifizierung allein mit der Immunofluoreszenz (IF) erreicht werden. Die vorgelegten Ergebnisse mit Antigenpräparationen verschiedener intestinaler <em>Bacteroides</em>-Arten (<em>B. fragilis, B. thetaiotaomicron, B. uniformis, B. ovatus</em> und <em>B. distasonis</em>) sowie mit den gegen diese Spezies hergestellten Immunseren weisen aus, daß die spezifische Erregerdarstellung sowohl mit der direkten als auch indirekten IF gelingt. Es zeigte sich jedoch, daß die direkte IF die brillantere Darstellung der Erreger ermöglicht. Unter Verwendung eines Konjugatpools der Immunoglobulinfraktionen wurden auch bei der Untersuchung klinischer Proben mit der direkten IF überzeugende Resultate erzielt (Sensitivität 88%, Spezifität 100%). Zum Schnellnachweis intestinaler <em>Bacteroides</em>-Arten mittels IF sollte daher der direkten Methode der Vorzug gegeben werden.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 492-502"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80020-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13793524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
The Bactec-system in the diagnosis of tuberculosis comparison of a conventional and the radiometric method (Bactec) for culturing, differentiation and susceptibility testing of Mycobacteria 将Bactec系统在结核病诊断中的常规方法与放射法(Bactec)进行分枝杆菌的培养、分化和药敏试验的比较
Johanna Presslich , Elisabeth Lahounik, Günther Kraus
{"title":"The Bactec-system in the diagnosis of tuberculosis comparison of a conventional and the radiometric method (Bactec) for culturing, differentiation and susceptibility testing of Mycobacteria","authors":"Johanna Presslich ,&nbsp;Elisabeth Lahounik,&nbsp;Günther Kraus","doi":"10.1016/S0176-6724(89)80019-7","DOIUrl":"10.1016/S0176-6724(89)80019-7","url":null,"abstract":"<div><p>A method developed in the USA (2, 6, 8) for the culturing, identification and differentiation of mycobacteria by means of a radiometric method (Bactec) was compared with a conventional method by examining 802 specimens received. Obviously better results were obtained with the new method: The number of positive cultures was 102(12.7%) for Bactec against 87 (10.8%) for the conventional method. When using the radiometric method, the contamination rate (5.1%) was higher than for the conventional method (3.1%). It would, however, seem that this disadvantage can be offset by an increase in the alkali concentration during pretreatment of the specimens. After elimination of all paired samples one or both specimens were found to be contaminated, 743 specimens remained for direct comparison. Of these, 101 (13.6%) were positive when the Bactec method, and 84 (11.3%), when the conventional method was used. The superiority of the new method was most obvious with sputum specimens: 14.5% were positive when Bactec, and 12.2%, when the conventional method was used. For the Bactec method, the mean period until positive results could be recognized by daily readings was 15 days against 28 days for the conventional method with weekly readings. Sensitivity testing can be completed within 8 days. Owing to the costs of the radiometric method, it is recommended to limit its use of defined situations.</p></div><div><p>Nach einem in den USA entwickelten Verfahren (2, 6, 8) zur Anzüchtung, Identifikation und Differenzierung von Mykobakterien mittels einer radiometrischen Methode (Bactec) wurde ein Vergleich zwischen dieser und einer konventionellen Methode bei 802 Einsendungen angestellt. Es zeigten sich deutlich bessere Resultate mit der neuen Methode, und zwar betrug die Zahl positiver Kulturen bei Bactec 102 (12,7%) gegenüber 87 (10,8%) bei der konventionellen Methode. Die Kontaminationsrate lag mit 5,1% bei der radiometrischen Methode höher als bei der konventionellen Methode (3,1%). Dieser Nachteil dürfte aber durch Erhöhung der Alkalikonzentration bei der Vorbehandlung der Proben zu reduzieren sein. Wenn man alle Probenpaare, bei denen eine oder beide Proben verunreinigt sind, eliminiert, stehen 743 Proben für den direkten Vergleich zur Verfügung. Von diesen Proben waren 101 (13,6%) positiv mit der Bactec-Methode und 84 (11,3) mit der konventionellen Methode. Bei Sputumproben war die Überlegenheit der neuen Methode am deutlichsten, mit 14,5% positiver Proben mit Bactec und 12,2% mit dem konventionellen Verfahren.</p><p>Die mittlere Dauer bis zur Erkennung positiver Ergebnisse betrug beim Bactec-Verfahren bei täglicher Ablesung 15 Tage gegenüber 28 Tagen bei der konventionellen Methode. Bei der letzteren Methode wurde wöchtenlich abgelesen. Eine Sensibilitätsprüfung kann nach 8 Tagen abgeschlossen sein.</p><p>Wegen der Kostspieligkeit der radiometrischen Methode empfiehlt sich eine genaue Abgrenzung ihrer Verwendung.</p></div>","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 487-491"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80019-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13642763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
II. Sachverzeichnis 2 . .Sachverzeichnis
{"title":"II. Sachverzeichnis","authors":"","doi":"10.1016/S0176-6724(89)80027-6","DOIUrl":"https://doi.org/10.1016/S0176-6724(89)80027-6","url":null,"abstract":"","PeriodicalId":101291,"journal":{"name":"Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology","volume":"270 4","pages":"Pages 547-549"},"PeriodicalIF":0.0,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0176-6724(89)80027-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137400905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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