Journal of Cartilage & Joint Preservation最新文献

{"title":"Postoperative imaging of cartilage: where are we in 2023?","authors":"Zachary E. Stewart ,&nbsp;F. Joseph Simeone ,&nbsp;Ali Guermazi ,&nbsp;Erik Berkson ,&nbsp;Mark Price ,&nbsp;Connie Y. Chang","doi":"10.1016/j.jcjp.2023.100150","DOIUrl":"10.1016/j.jcjp.2023.100150","url":null,"abstract":"<div><p>Imaging, particularly magnetic resonance imaging, and cartilage restoration surgery have both made substantial progress since the beginning of the 21st century. This era has seen high-field (3 T) magnets become ubiquitous in large centers, the development of novel quantitative imaging tools, and the advent of new generations of autologous cartilage implantation techniques. Here, we provide an update on postoperative imaging after cartilage restoration surgeries.</p><p>Postoperative magnetic resonance imaging of cartilage restorative procedures is used for both confirming the success of the repair and identifying potential complications. There are no standard protocols for postoperative imaging following cartilage restorative procedures; however, in general, imaging can be routinely used 3 to 6 months postoperatively to assess for integration of repair and, depending on the technique, at approximately 6 to 12 months to assess for defect filling, integration with the host, and maturation of repair tissue. Imaging permits longitudinal assessments of cartilage repair and the adjacent osteochondral unit and is essential to assess the success of cartilage restoration.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"4 1","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000562/pdfft?md5=804d137b9d83c1e1969ec429e26a3760&pid=1-s2.0-S2667254523000562-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123187372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Similar short-term results between scaffold implanted mesenchymal stem cells vs acellular scaffolds with concentrated bone marrow aspirate augmentation for the repair of chondral defects of the knee: evidence from a meta-analysis","authors":"Zachariah Gene Wing Ow ,&nbsp;Katelyn Kaye-Ling Lim ,&nbsp;Shant Qinxiang Sin ,&nbsp;Hannah Li Xin Cheang ,&nbsp;Dean Wang ,&nbsp;Denny Tjiauw Tjoen Lie ,&nbsp;James Hoi Po Hui ,&nbsp;Keng Lin Wong","doi":"10.1016/j.jcjp.2023.100128","DOIUrl":"10.1016/j.jcjp.2023.100128","url":null,"abstract":"<div><h3>Background</h3><p>Scaffold-implanted mesenchymal stem cells (MSCs) are gaining popularity for the treatment of cartilage defects. However, there is little evidence comparing its efficacy against the currently well-established technique of utilizing acellular scaffolds (ACs) with concentrated bone marrow aspirate (cBMA) for treating knee chondral defects.</p></div><div><h3>Objectives</h3><p>To compare scaffold-implanted MSCs against ACs with cBMA for the repair of knee cartilage lesions.</p></div><div><h3>Data Sources</h3><p>Medline and Embase.</p></div><div><h3>Study Eligibility Criteria, Participants, and Interventions</h3><p>Inclusion: (1) Studies involving patients with high grade chondral lesions on the tibiofemoral or patellofemoral articular surfaces, (2) studies involving patients that received either scaffold-implanted MSCs or ACs for treatment, and (3) studies with postoperative patient follow-up of at least 1 year.</p></div><div><h3>Study Appraisal and Synthesis Methods</h3><p>Single-arm meta-analysis of studies reporting patient-reported outcome measures, reoperation and incomplete filling rates was performed.</p></div><div><h3>Results</h3><p>Degree of postoperative International Knee Documentation Committee score improvement in patients receiving ACs augmented with cBMA was significantly higher compared to those receiving scaffold-implanted MSCs (<em>P &lt;</em> .01). Additionally, patients receiving ACs with cBMA augmentation were at significantly lower risk of incomplete defect filling when compared to patients receiving either scaffold-implanted MSCs (<em>P</em> &lt; .01), or patients receiving non-cBMA augmented ACs (<em>P</em> &lt; .01).</p></div><div><h3>Conclusion</h3><p>This meta-analysis demonstrates that cartilage repair with ACs with cBMA augmentation yields marginally better short-term outcomes when compared to scaffold-implanted MSCs. This suggests that even with the advent of MSCs increasing in availability as a treatment modality, ACs with cBMA augmentation remains as a competitive, cost-effective option for cartilage repair.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100128"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000331/pdfft?md5=438d29489865046824105eaf2b2f611e&pid=1-s2.0-S2667254523000331-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127180269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma is similar to platelet-rich plasma plus hyaluronic acid for the treatment of knee osteoarthritis at 2 years: a randomized controlled trial","authors":"Eric A. Branch ,&nbsp;Joshua J. Cook ,&nbsp;Achraf Cohen ,&nbsp;Hillary Plummer ,&nbsp;Alireza Emami ,&nbsp;Jessica Truett ,&nbsp;Adam W. Anz","doi":"10.1016/j.jcjp.2023.100129","DOIUrl":"10.1016/j.jcjp.2023.100129","url":null,"abstract":"<div><h3>Introduction</h3><p>Autologous platelet-rich plasma (PRP) and hyaluronic acid (HA) are common treatments for knee osteoarthritis (OA). Several studies have demonstrated PRP as a safe and effective treatment for OA and, in some studies, produces a better outcome than HA.</p></div><div><h3>Objective</h3><p>The primary objective of this study was to determine if HA injected with PRP would improve the efficacy of treatment of symptomatic knee OA compared to PRP alone. We hypothesized that the addition of HA would improve the efficacy of (PRP).</p></div><div><h3>Methods</h3><p>A total of 64 participants with Kellgren-Lawrence (KL) Grades 1 to 4 knee OA were randomized into 2 groups and scheduled to receive 3-injections. Group 1 [PRP] received injections of PRP. Group 2 [HA+PRP] received the same treatment, with an additional HA injection for the first 2 injections. Both groups were blindfolded for the first 2 injections. To evaluate the efficacy of the regimen, participants completed patient reported outcome measures (PROMs). PROMs were completed prior to injections and at 1, 3, 6, 12, 18, and 24 months after the final injection.</p></div><div><h3>Results</h3><p>Participants in both groups felt pain consistent with standard-of-care HA injection regimens with no difference in perceived pain. No infections or complications were observed. All PROM scores demonstrated improvement over baseline from 1-24 months post injections. There were no statistically significant differences between the groups.</p></div><div><h3>Conclusions</h3><p>For the treatment of OA, PRP alone or PRP concomitantly with HA performed similarly out to 24 months. PRP in combination with HA was not superior to PRP alone.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100129"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000343/pdfft?md5=9e38ffee59b44bdf24b467c2044e8673&pid=1-s2.0-S2667254523000343-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126562414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes","authors":"Haixiang Liang ,&nbsp;Dan Li ,&nbsp;Eric V. Neufeld ,&nbsp;Michael J. Sayegh ,&nbsp;Adam Kiridly ,&nbsp;Pablo Palacios ,&nbsp;Henintsoa Fanjaniaina Andriamifidy ,&nbsp;Pooja Swami ,&nbsp;Kenneth R. Zaslav ,&nbsp;Nicholas A. Sgaglione ,&nbsp;Daniel A. Grande","doi":"10.1016/j.jcjp.2023.100146","DOIUrl":"10.1016/j.jcjp.2023.100146","url":null,"abstract":"<div><h3>Introduction</h3><p>Extracellular vesicles (EVs) released by mesenchymal stem cells (MSCs) are theorized to mediate cartilage regeneration through paracrine actions. It is hypothesized that EVs derived from synovial fluid MSCs (SF-MSCs) will promote anabolic gene expressions and decrease catabolic markers of in vitro osteoarthritic chondrocytes. These EVs will also demonstrate increased cartilage regeneration as measured in a rat osteoarthritis (OA) model.</p></div><div><h3>Objectives</h3><p>This study tested the in-vitro anti-inflammation effects of using EVs from SF-MSCs on chondrocytes and in vivo study on chondroprotective effects of EVs with OA environment.</p></div><div><h3>Methods</h3><p>In vitro, rat chondrocytes were exposed to EVs collected from SF-MSC that had been either exposed or unexposed to interleukin-1β (IL-1β). Gene expressions for various anabolic and catabolic markers were measured. In vivo, rats who received anterior cruciate ligament transection as an OA model with an osteoarthritic knee joint were exposed to weekly injections of SF-MSC-derived EVs at 2 different doses. The results were evaluated with histological study and scored using the Osteoarthritis Research Society International system. Serum cytokines were analyzed via enzyme-linked immunosorbent assay.</p></div><div><h3>Results</h3><p>In vitro, chondrocytes exposed to SF-MSCs derived EVs demonstrated increased anabolic markers and decreased catabolic markers also with the EVs collected from interleukin-1β treated SF-MSCs. In vivo, rats that received EVs displayed lower Osteoarthritis Research Society International scores, more collagen type II alpha 1, and less matrix metalloproteinase-13 than the control groups. This anti-inflammation potential was systemic, as demonstrated by the decrease of serum proinflammatory cytokines.</p></div><div><h3>Conclusions</h3><p>SF-MSCs-derived EVs were effective at promoting the expression of anabolic markers while simultaneously attenuating the production of proinflammatory markers in vitro and in vivo. These anti-inflammation effects were dose-dependent in vivo. The injection of EVs in the knee joint changed the cytokines in the serum.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100146"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000525/pdfft?md5=d3597df5e39a6b8e3b0bbc0f354dcfdd&pid=1-s2.0-S2667254523000525-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122508222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondromalacia patellae: Cartilaginous lesions of the patella in the setting of anterior knee pain","authors":"Benjamin J. Levy ,&nbsp;Charles Cossell ,&nbsp;Nathan L. Grimm ,&nbsp;Andrew E. Jimenez ,&nbsp;Leslie J. Bisson","doi":"10.1016/j.jcjp.2022.100096","DOIUrl":"10.1016/j.jcjp.2022.100096","url":null,"abstract":"<div><h3>Introduction</h3><p>Chondromalacia patellae (CP) is common among patients with anterior knee pain (AKP), characterized by softening and deterioration of the patellar cartilage. Although the exact etiology is unknown, risk factors include lower extremity muscle weakness, obesity, and female sex. Further, patellar maltracking may play a role in developing CP. Treatment algorithms for CP remain vague and inconsistent as the significance of the diagnosis remains in question. Initial treatment is typically nonoperative with various adjunct modalities. However, surgery may have a role for recalcitrant cases.</p></div><div><h3>Objectives</h3><p>Although CP is a common diagnosis, there is still much to be learned about the significance of this diagnosis and subsequent treatment strategies. Knowledge of this will help to guide providers moving forward.</p></div><div><h3>Methods</h3><p>The authors completed a thorough literature search of available published work through December 31, 2021, discussing CP and its relationship with AKP, and distilled this information into current concepts regarding history, diagnosis, treatment, and future directions.</p></div><div><h3>Results</h3><p>Although much is still unknown about the significance and best treatment strategy for patients with AKP pain and CP, it is likely that in many cases chondromalacia is a finding in the presence of AKP, rather than the cause of the AKP.</p></div><div><h3>Conclusions</h3><p>More work must be done to fully elucidate the underlying causes of CP. However, some of these cases are likely related to subtle maltracking and perhaps can be surgically corrected. In most cases, extensive nonoperative measures and searching for other causes of AKP is most prudent.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100096"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254522000592/pdfft?md5=f4d3bf0babecfa4a6133386ee393cc70&pid=1-s2.0-S2667254522000592-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134205693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging evaluation of physical activity effect on knee articular cartilage","authors":"Marcus Vinicius Danieli ,&nbsp;Arnaldo Eder Kist ,&nbsp;Luan Possani Rodrigues ,&nbsp;Ian Walter Stegmann Filho ,&nbsp;Arthur Gomes Rossito ,&nbsp;Felipe Santos Lima ,&nbsp;João Luiz Francisconi ,&nbsp;Paulo Roberto Bignardi ,&nbsp;Carlos Eduardo Abascal Shiguihara ,&nbsp;João Paulo Fernandes Guerreiro","doi":"10.1016/j.jcjp.2023.100138","DOIUrl":"10.1016/j.jcjp.2023.100138","url":null,"abstract":"<div><h3>Introduction</h3><p>Hyaline cartilage is a complex tissue with great load absorbing-function. Studies showed that moderate exercise can have a positive effect on chondral matrix composition but excessive can be harmful to cartilage.</p></div><div><h3>Objectives</h3><p>Evaluate knee cartilage thickness before and after physical activity (running) in healthy young individuals.</p></div><div><h3>Methods</h3><p>Ten healthy young adult male volunteers aged between 18 and 21 years old were selected to undergo magnetic resonance imaging examination of the right knee to measure the cartilage thickness of the anterior, medial, and lateral compartments before and after 1 hour of continuous running. Cartilage thickness measurements were performed by a radiologist who did not know the identity of the volunteers or the moment of the evaluation (before or after the exercise).</p></div><div><h3>Results</h3><p>The subjects' mean age, height, and weight were 19.5 years, 1.80 m, and 75.2 kg, respectively, with a mean body mass index of 23.14. There was a decrease in chondral thickness after running on all surfaces evaluated. The changes that were relevant (<em>P</em> &lt; .05) were all the medial femoral condyle, the anterior portion of the lateral femoral condyle, the anterior portion of the lateral plateau, the entire surface of the patella and trochlea. The greatest difference in thickness between before and after exercise was observed in the lateral facet of the patella (−0.224 mm) followed by the anterior surface of the medial femoral condyle (−0.186 mm). In proportional values, the highest decrease occurred in the anterior portion of the medial femoral condyle (−11.42%).</p></div><div><h3>Conclusion</h3><p>There was a decrease in knee chondral thickness after 60 minutes of running, being statistically significant in the medial femoral condyle, anterior surface of the lateral femoral condyle, and lateral plateau and patellofemoral joint (patella and trochlea).</p></div><div><h3>Level of evidence</h3><p>IV (Case Series).</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000446/pdfft?md5=0446ff4842db4198fd54cc93b7acc8b4&pid=1-s2.0-S2667254523000446-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133515374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MACI (autologous cultured chondrocytes on porcine collagen membrane) in patients 40 years and older: short-term clinical outcomes and patient satisfaction","authors":"Liana Leja ,&nbsp;Tom Minas","doi":"10.1016/j.jcjp.2023.100102","DOIUrl":"10.1016/j.jcjp.2023.100102","url":null,"abstract":"<div><h3>Introduction</h3><p>Cartilage restoration in the middle-aged population (40-60 years) is understudied.</p></div><div><h3>Objectives</h3><p>The purpose of this study is to report the clinical outcomes and satisfaction of patients over the age of 40 years old who underwent autologous cultured chondrocytes on porcine collagen membrane (MACI) for the treatment of degenerative articular cartilage lesions in the knee.</p></div><div><h3>Methods</h3><p>The study included 30 patients who were over 40 years of age (mean 48.6 ± 5.5 years, range 41-60 years). Patients included were treated by a single surgeon from 2017 to 2020. Clinical outcome data was available for 29 patients at a minimum 2-year follow-up (mean 39.6 ± 9.6 months). All patients with intact MACI grafts completed a satisfaction questionnaire. The mean lesion surface area transplanted was 11.5 ± 6.6 cm². The mean number of defects transplanted was 2.5 ± 1.0. Treatment failure was defined as delamination of the MACI graft with associated clinical symptoms and/or progression of osteoarthritis.</p></div><div><h3>Results</h3><p>Of the 29 patients, 6 (21%) had a graft failure. Three patients went on to osteochondral allograft transplantation, 1 patient experienced symptomatic improvement with debridement, and 2 patients underwent total knee arthroplasty. Twenty-seven of the 29 patients (93%) maintained their native knee. Of those with intact MACI grafts, 17 out of 23 (74%) rated their knee as “good” or “excellent” and 16 out of 23 (70%) were satisfied with their surgery.</p></div><div><h3>Conclusions</h3><p>MACI is effective in the short-term in patients over the age of 40 years old with degenerative articular cartilage lesions to maintain their native knee. A moderate degree of satisfaction was achieved in most patients.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000045/pdfft?md5=b7016bbc6aca30057b50040000625354&pid=1-s2.0-S2667254523000045-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123082411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-rich plasma induced healing of a chronic osteochondral defect of the knee","authors":"Michael R. Baria ,&nbsp;Ryan Barnes ,&nbsp;David Flanigan ,&nbsp;Christopher Kaeding","doi":"10.1016/j.jcjp.2023.100132","DOIUrl":"10.1016/j.jcjp.2023.100132","url":null,"abstract":"<div><h3>Introduction</h3><p>Platelet-rich plasma (PRP) has increasingly been used in sports medicine to promote tissue healing. No previous study has demonstrated the effect of PRP alone for treatment of osteochondral defects of the knee.</p></div><div><h3>Case presentation</h3><p>We describe the case of a 37-year-old male who was diagnosed with osteochondritis dissecans of the medial femoral condyle in 2000. At that time, he underwent arthroscopic repair. In 2018, he presented for increased medial knee pain and catching. He elected to proceed with an initial PRP injection in 2018 and returned for 3 more injections through 2021. After the 4 injections, magnetic resonance imaging (MRI) demonstrated interval healing of the osteochondral defect with markedly smaller dimensions than the original 2018 MRI [0.7 cm × 0.5 cm].</p></div><div><h3>Conclusion</h3><p>To our knowledge, this is the first case of MRI-proven healing of an osteochondral defect with PRP (without accompanying cartilage restoration surgery). Ongoing treatment with an anabolic therapy like PRP may facilitate symptom improvement and structural healing.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000379/pdfft?md5=b58d595182e70ded7deca747607594f3&pid=1-s2.0-S2667254523000379-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123828638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gap-filling, regenerative implant for open-wedge osteotomy","authors":"Margot Rikkers ,&nbsp;H. Chien Nguyen ,&nbsp;Nasim Golafshan ,&nbsp;Mylène de Ruijter ,&nbsp;Riccardo Levato ,&nbsp;Lucienne A. Vonk ,&nbsp;Nienke van Egmond ,&nbsp;Miguel Castilho ,&nbsp;Roel J.H. Custers ,&nbsp;Jos Malda","doi":"10.1016/j.jcjp.2023.100117","DOIUrl":"10.1016/j.jcjp.2023.100117","url":null,"abstract":"<div><h3>Introduction</h3><p>In patients suffering from unilateral osteoarthritis in the knee, an osteotomy can provide symptomatic relief and postpone the need for replacement of the joint. Nevertheless, open-wedge osteotomies (OWOs) around the knee joint face several challenges like postoperative pain and bone nonunion.</p></div><div><h3>Objectives</h3><p>In this study, the aim was to design, fabricate, and evaluate a gap-filling implant for OWO using an osteoinductive and degradable biomaterial.</p></div><div><h3>Methods</h3><p>Design of porous wedge-shaped implants was based on computed tomography scans of cadaveric legs. Implants were 3-dimensionally printed using a magnesium strontium phosphate-polycaprolactone (MgPSr-PCL) biomaterial ink. Standardized scaffolds with different inter-fiber spacing (IFS) were mechanically characterized and osteoinductive properties of the biomaterial were assessed in vitro. Finally, human-sized implants with different heights (5 mm, 10 mm, 15 mm) were designed and fabricated for ex vivo implantation during 3 OWO procedures in human cadaveric legs.</p></div><div><h3>Results</h3><p>Implants printed with an interior of IFS-1.0 resulted in scaffolds that maintained top and bottom porosity, while the interior of the implant exhibited significant mechanical stability. Bone marrow concentrate and culture expanded mesenchymal stromal cells attached to the MgPSr-PCL material and proliferated over 21 days in culture. The production of osteogenic markers alkaline phosphatase activity, calcium, and osteocalcin was promoted in all culture conditions, independent of osteogenic induction medium. Finally, 3 OWO procedures were planned and fabricated wedges were implanted ex vivo during the procedures. A small fraction of one side of the wedges was resected to assure fit into the proximal biplanar osteotomy gap. Preplanned wedge heights were maintained after implantation as measured by micro-computed tomography.</p></div><div><h3>Conclusion</h3><p>To conclude, personalized implants for implantation in OWOs were successfully designed and manufactured. The implant material supported osteogenesis of mesenchymal stromal cells and bone marrow concentrate in vitro and full-size implants were successfully implemented into the surgical procedure without compromising preplanned wedge height.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000197/pdfft?md5=a5e4085339c07797afaa60e8703f80de&pid=1-s2.0-S2667254523000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133335155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteochondral allograft and xenograft immunogenicity decrease following ex vivo tissue culture","authors":"Kate E. Birdwhistell ,&nbsp;Samuel P. Franklin ,&nbsp;David J. Hurley ,&nbsp;Bradley D. Heins ,&nbsp;John F. Peroni","doi":"10.1016/j.jcjp.2023.100115","DOIUrl":"10.1016/j.jcjp.2023.100115","url":null,"abstract":"<div><h3>Introduction/Objective</h3><p>Osteochondral allografting can be an effective option for the treatment of focal cartilage injuries. However, wide scale applicability of this technique is limited by availability of donor tissue. To overcome this limitation, osteochondral xenografting has been proposed as a potential alternative, but immune-mediated rejection precludes its clinical application at this time. We hypothesized that removing much of the marrow cell content through tissue culture would result in a decrease in xenograft immunogenicity. We believed this could be tested in a preclinical animal model.</p></div><div><h3>Methods</h3><p>Fresh porcine femoral condyles were harvested and cultured for 0, 14, or 28 days before coculture with either allogeneic enriched porcine T-cells or xenogeneic enriched equine T-cells for 5 days. After 5 days, these cocultures were pulsed with 5-ethynyl-2′deoxyuridine then analyzed by flow cytometry to assess the level of cell proliferation. Coculture supernatant samples were also collected to evaluate the levels of prostaglandin-E2 and monocyte chemotactic protein-1 (MCP-1) as markers of inflammation.</p></div><div><h3>Results</h3><p>The osteochondral tissue induced significantly less T-cell proliferation, prostaglandin-E2, and MCP-1 production when the tissue had been cultured for either 14 or 28 days prior to the coculture assay (<em>P</em> &lt; .0001).</p></div><div><h3>Conclusions</h3><p>We conclude that T-cell responses to either allogeneic or xenogeneic osteochondral tissues were significantly reduced by 14 days of preculture prior to exposure to T-cells.</p></div>","PeriodicalId":100760,"journal":{"name":"Journal of Cartilage & Joint Preservation","volume":"3 4","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667254523000173/pdfft?md5=08e4533d0f9ff6b3ca8661fb07facc17&pid=1-s2.0-S2667254523000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123805830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}