工程技术最新文献

{"title":"Genome editing in Sub-Saharan Africa: a game-changing strategy for climate change mitigation and sustainable agriculture.","authors":"Peter Amoah, Abdoul-Razak Oumarou Mahamane, Moise Hubert Byiringiro, Neo Jeremiah Mahula, Nyimasata Manneh, Yetunde Ruth Oluwasegun, Abebawork Tilahun Assfaw, Hellen Mawia Mukiti, Abubakar Danlami Garba, Felicity Kido Chiemeke, Omena Bernard Ojuederie, Bunmi Olasanmi","doi":"10.1080/21645698.2024.2411767","DOIUrl":"10.1080/21645698.2024.2411767","url":null,"abstract":"<p><p>Sub-Saharan Africa's agricultural sector faces a multifaceted challenge due to climate change consisting of high temperatures, changing precipitation trends, alongside intensified pest and disease outbreaks. Conventional plant breeding methods have historically contributed to yield gains in Africa, and the intensifying demand for food security outpaces these improvements due to a confluence of factors, including rising urbanization, improved living standards, and population growth. To address escalating food demands amidst urbanization, rising living standards, and population growth, a paradigm shift toward more sustainable and innovative crop improvement strategies is imperative. Genome editing technologies offer a promising avenue for achieving sustained yield increases while bolstering resilience against escalating biotic and abiotic stresses associated with climate change. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein (CRISPR/Cas) is unique due to its ubiquity, efficacy, alongside precision, making it a pivotal tool for Sub-Saharan African crop improvement. This review highlights the challenges and explores the prospect of gene editing to secure the region's future foods.</p>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":"15 1","pages":"279-302"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Golden opportunities? How marketing expectations drive purchase intentions of golden rice in Bangladesh and the Philippines.","authors":"Dilshad Zahan Ethen, Maimuna Begum, Berre Deltomme, Md Rasheduzzaman, Josefina F Ballesteros, Riza Abilgos-Ramos, Mohammad Jahangir Alam, Alice Onek Atimango, Hans De Steur","doi":"10.1080/21645698.2024.2418161","DOIUrl":"https://doi.org/10.1080/21645698.2024.2418161","url":null,"abstract":"<p><p>Golden Rice (GR), genetically modified (GM) rice enriched with provitamin A, holds promise to address micronutrient deficiencies in developing countries. However, its success hinges on market acceptance. This study investigates how the marketing aspects of GR influence consumers' purchase intentions in Bangladesh and the Philippines. The Expectation Confirmation Theory (ECT) is employed to analyze the role of expectations regarding the marketing mix components (i.e. product, price, place, promotion), risk perceptions, performance expectations, and expected satisfaction on consumers' purchase intentions. Data from online surveys in Bangladesh (<i>n</i> = 391) and the Philippines (<i>n</i> = 354), collected using convenience sampling, were analyzed using structural equation modeling. Findings reveal that positive expectations toward the marketing mix, performance, and satisfaction increase consumers' purchase intention of GR, whereas risk perceptions have a negative influence. Additionally, it was found that expectations toward all four marketing mix components significantly affect purchase intention in Bangladesh. However, only product and promotion have a notable influence in the Philippines. These results emphasize the importance of effectively addressing consumers' marketing expectations to help ensure a successful implementation. This study is novel as it delves into consumers' purchase intentions for a GM biofortified crop and their expectations for different aspects of its future marketing (i.e. product, price, place, promotion), performance, and satisfaction. If GR is commercialized, future research should validate these expectations based on actual consumer experiences. Additionally, longitudinal studies could track changes in consumer expectations over time, identifying consistently valued marketing elements and offering a valuable technique for product development before launch.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"316-335"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing the impact of CPSF30 gene disruption on TuMV infection in <i>Arabidopsis thaliana</i>.","authors":"Yanping Wei, Quan Yuan, Dalal Sulaiman Alshaya, Abdul Waheed, Kotb A Attia, Sajid Fiaz, Muhammad Shahid Iqbal","doi":"10.1080/21645698.2024.2403776","DOIUrl":"10.1080/21645698.2024.2403776","url":null,"abstract":"<p><p>CPSF30, a key polyadenylation factor, also serves as an m⁶A reader, playing a crucial role in determining RNA fate post-transcription. While its homologs mammals are known to be vital for viral replication and immune evasion, the full scope of CPSF30 in plant, particular in viral regulation, remains less explored. Our study demonstrates that CPSF30 significantly facilitates the infection of turnip mosaic virus (TuMV) in <i>Arabidopsis thaliana</i>, as evidenced by infection experiments on the engineered <i>cpsf30</i> mutant. Among the two isoforms, CPSF30-L, which were characterized with m⁶A binding activity, emerged as the primary isoform responding to TuMV infection. Analysis of m⁶A components revealed potential involvement of the m⁶A machinery in regulating TuMV infection. In contrast, CPSF30-S exhibited distinct subcellular localization, coalescing with P-body markers (AtDCP1 and AtDCP2) in cytoplasmic granules, suggesting divergent regulatory mechanisms between the isoforms. Furthermore, comprehensive mRNA-Seq and miRNA-Seq analysis of Col-0 and <i>cpsf30</i> mutants revealed global transcriptional reprogramming, highlighting CPSF30's role in selectively modulating gene expression during TuMV infection. In conclusion, this research underscores CPSF30's critical role in the TuMV lifecycle and sets the stage for further exploration of its function in plant viral regulation.</p>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":"15 1","pages":"1-17"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructure and properties of underwater in-situ wire-based laser additive manufactured duplex stainless steel","authors":"Congwei Li, Jialei Zhu, Caimei Wang, Caiyan Deng, Lei Cui, Xiaochun Zhang, Chenglu Zhao, Xiangdong Jiao","doi":"10.1080/17452759.2024.2401925","DOIUrl":"https://doi.org/10.1080/17452759.2024.2401925","url":null,"abstract":"A novel underwater in-situ wire-based laser additive manufacturing (ULAM) technology is proposed for the in-service repair of underwater components in nuclear power plant. Duplex stainless steel (DSS) obtained in air and underwater environments were analysed using material characterisation and testing methods. The effects of underwater additive environments on the microstructure evolution, mechanical properties and corrosion resistance of the specimens were investigated. The results show that the laser heat input is consumed to balance the heat loss of the water-cooled base material during the underwater laser additive manufacturing process, leading to a reduction in the heat input to the molten pool. Underwater specimen exhibit a two-phase balance, with small ferrite grain boundary angles, resulting in better tensile strength and corrosion resistance. Laser reheat treatment leads to a phase change in microstructure, which can enhance the microhardness and the tensile strength. The ULAM system can meet the requirements of actual engineering for cladding layer.","PeriodicalId":10,"journal":{"name":"ACS Central Science","volume":"14 1","pages":""},"PeriodicalIF":10.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An environmental risk assessment of IPD079Ea: a protein derived from <i>Ophioglossum pendulum</i> with activity against <i>Diabrotica</i> spp.In maize.","authors":"Bridget F O'Neill, Chad Boeckman, Kristine LeRoy, Chris Linderblood, Taylor Olson, Rachel Woods, Mary Challender","doi":"10.1080/21645698.2023.2299503","DOIUrl":"10.1080/21645698.2023.2299503","url":null,"abstract":"<p><p>Farmers in North America face significant pressure from insects in their maize fields, particularly from corn rootworm (<i>Diabrotica</i> spp.). Research into proteins capable of insecticidal activity has found several produced by ferns. One protein, IPD079Ea, was derived from <i>Ophioglossum pendulum</i> and has shown activity against corn rootworm. An environmental risk assessment was conducted for maize event DP-915635-4, which provides control of corn rootworms via expression of the IPD079Ea protein. This assessment focused on IPD079Ea and characterized potential exposure and hazard to non-target organisms (NTOs). For exposure, estimated environmental concentrations (EECs) were calculated. For hazard, laboratory dietary toxicity studies were conducted with IPD079Ea and surrogate non-target organisms. Environmental risk was characterized by comparing hazard and exposure to calculate the margin of exposure (MOE). Based on the MOE values for DP-915635-4 maize, the IPD079Ea protein is not expected to result in unreasonable adverse effects on beneficial NTO populations at environmentally relevant concentrations.</p>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":"15 1","pages":"15-31"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from TNF-α-treated bone marrow mesenchymal stem cells ameliorate myocardial infarction injury in mice.","authors":"Shuo Wang, Rubin Wu, Qincong Chen, Tao Liu, Liu Li","doi":"10.1080/15476278.2024.2356341","DOIUrl":"10.1080/15476278.2024.2356341","url":null,"abstract":"<p><p>Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) exhibit considerable therapeutic potential for myocardial regeneration. In our investigation, we delved into their impact on various aspects of myocardial infarction (MI), including cardiac function, tissue damage, inflammation, and macrophage polarization in a murine model. We meticulously isolated the exosomes from TNF-α-treated BMSCs and evaluated their therapeutic efficacy in a mouse MI model induced by coronary artery ligation surgery. Our comprehensive analysis, incorporating ultrasound, serum assessment, Western blot, and qRT-PCR, revealed that exosomes from TNF-α-treated BMSCs demonstrated significant therapeutic potential in reducing MI-induced injury. Treatment with these exosomes resulted in improved cardiac function, reduced infarct area, and increased left ventricular wall thickness in MI mice. On a mechanistic level, exosome treatment fostered M2 macrophage polarization while concurrently suppressing M1 polarization. Hence, exosomes derived from TNF-α-treated BMSCs emerge as a promising therapeutic strategy for alleviating MI injury in a mouse model.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":"20 1","pages":"2356341"},"PeriodicalIF":1.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best practices for acceptability of GM crops field trials conclusions: lessons for Africa.","authors":"Paul Chege, Julia Njagi, John Komen, Godfrey Ngure, John Muriuki, Margaret Karembu","doi":"10.1080/21645698.2024.2376415","DOIUrl":"10.1080/21645698.2024.2376415","url":null,"abstract":"<p><p>The ability to transfer information about the performance, safety, and environmental impacts of a genetically modified (GM) crop from confined field trials (CFTs) conducted in one location to another is increasingly gaining importance in biosafety regulatory assessment and decision-making. The CFT process can be expensive, time-consuming, and logistically challenging. Data transportability can help overcome these challenges by allowing the use of data obtained from CFTs conducted in one country to inform regulatory decision-making in another country. Applicability of transported CFT data would be particularly beneficial to the public sector product developers and small enterprises that develop innovative GM events but cannot afford to replicate redundant CFTs, as well as regulatory authorities seeking to improve the deployment of limited resources. This review investigates case studies where transported CFT data have successfully been applied in biosafety assessment and decision-making, with an outlook of how African countries could benefit from a similar approach.</p>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":"15 1","pages":"222-232"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11236291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of media reportage on GMOs in Ghana following approval of first GM crop.","authors":"Joseph Opoku Gakpo, Dennis Baffour-Awuah","doi":"10.1080/21645698.2024.2365481","DOIUrl":"10.1080/21645698.2024.2365481","url":null,"abstract":"<p><p>Ghana's parliament in 2011 passed the Biosafety Act to allow for the application of genetically modified organism (GMO) technology in the country's agriculture. In a vibrant democracy, there have been extensive media discussions on whether GM crops will benefit or harm citizens. In June 2022, the state GMO regulator, the National Biosafety Authority (NBA), approved the country's first GM crop (Bt cowpea) for environmental release, declaring the crop does not present an altered environmental risk or a food/feed safety concern. This study identified 3 of the country's most vibrant digital news outlets and did a content analysis of all GMO stories reported 18 months pre- and post-approval to assess whether the approval changed the focus of GMO issues the media reports on. 91 articles were identified. The results show media reports on the likely impact of GMOs on the country's food security shot up after the approval. However, media reports on the possible health, sociocultural, and environmental impact of GMOs declined. We observe the media and the public appear interested in deliberations on how the technology could address or worsen food insecurity and urge agricultural biotechnology actors in Ghana to focus on that in their sensitization activities.</p>","PeriodicalId":4,"journal":{"name":"ACS Applied Energy Materials","volume":"15 1","pages":"16-27"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Adipose Tissue-Derived Stromal Cells Ameliorate Adriamycin-Induced Nephropathy by Promoting Angiogenesis.","authors":"Xiaodi Zhao, Chengyan Ma, Lijie Li, Yuemei Yang, Sen Zhang, Xiaoli Wang","doi":"10.1080/15476278.2024.2356339","DOIUrl":"10.1080/15476278.2024.2356339","url":null,"abstract":"<p><p>This study is to investigate the therapeutical effect and mechanisms of human-derived adipose mesenchymal stem cells (ADSC) in relieving adriamycin (ADR)-induced nephropathy (AN). SD rats were separated into normal group, ADR group, ADR+Losartan group (20 mg/kg), and ADR + ADSC group. AN rats were induced by intravenous injection with adriamycin (8 mg/kg), and 4 d later, ADSC (2 × 10⁵ cells/mouse) were administrated twice with 2 weeks interval time (i.v.). The rats were euthanized after the 6 weeks' treatment. Biochemical indicators reflecting renal injury, such as blood urea nitrogen (BUN), neutrophil gelatinase alpha (NGAL), serum creatinine (Scr), inflammation, oxidative stress, and pro-fibrosis molecules, were evaluated. Results demonstrated that we obtained high qualified ADSCs for treatment determined by flow cytometry, and ADSCs treatment significantly ameliorated renal injuries in DN rats by decreasing BUN, Scr and NGAL in peripheral blood, as well as renal histopathological injuries, especially protecting the integrity of podocytes by immunofluorescence. Furthermore, ADSCs treatment also remarkably reduced the renal inflammation, oxidative stress, and fibrosis in DN rats. Preliminary mechanism study suggested that the ADSCs treatment significantly increased renal neovascularization via enhancing proangiogenic VEGF production. Pharmacodynamics study using in vivo imaging confirmed that ADSCs via intravenous injection could accumulate into the kidneys and be alive at least 2 weeks. In a conclusion, ADSC can significantly alleviate ADR-induced nephropathy, and mainly through reducing oxidative stress, inflammation and fibrosis, as well as enhancing VEGF production.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":"20 1","pages":"2356339"},"PeriodicalIF":1.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11135856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?","authors":"Sophia Edelmann, Christian Savini, Dominik Moor, Jörn Lämke, Kathrin Lieske, Marco Mazzara, Hendrik Emons, Joachim Mankertz, Christopher Weidner","doi":"10.1080/21645698.2024.2423441","DOIUrl":"10.1080/21645698.2024.2423441","url":null,"abstract":"<p><p>Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"352-360"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}