IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-11-14 DOI: 10.1080/21645698.2024.2423441

Sophia Edelmann, Christian Savini, Dominik Moor, Jörn Lämke, Kathrin Lieske, Marco Mazzara, Hendrik Emons, Joachim Mankertz, Christopher Weidner

{"title":"Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?","authors":"Sophia Edelmann, Christian Savini, Dominik Moor, Jörn Lämke, Kathrin Lieske, Marco Mazzara, Hendrik Emons, Joachim Mankertz, Christopher Weidner","doi":"10.1080/21645698.2024.2423441","DOIUrl":"10.1080/21645698.2024.2423441","url":null,"abstract":"Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"352-360"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consumers' response to genetically modified food: an Italian case study. 消费者对转基因食品的反应：意大利案例研究。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-11-06 DOI: 10.1080/21645698.2024.2417473

Federica DeMaria, Simona Romeo Lironcurti, Federica Morandi, Alessandra Pesce, Laura Gazza, Francesca Nocente

{"title":"Consumers' response to genetically modified food: an Italian case study.","authors":"Federica DeMaria, Simona Romeo Lironcurti, Federica Morandi, Alessandra Pesce, Laura Gazza, Francesca Nocente","doi":"10.1080/21645698.2024.2417473","DOIUrl":"10.1080/21645698.2024.2417473","url":null,"abstract":"The agricultural sector could benefit from biotechnologies in addressing challenges such as pests, droughts, and food supply issues. Genetically modified (GM) crops have been developed to offer not only economic advantages to farmers but also to contribute positively to the environment, human health, and consumer well-being. However, consumers' hesitancy in buying GM food may stem from societal reactions to how biotechnologies in agriculture have been regulated so far. The legislative debate that led, in early 2024, to the approval of Commission's proposal (COM(2023) 411 final) - aimed at simplifying the authorization process for plants obtained with certain new genomic techniques (NGTs) - has sparkled public discussion in the European Union on the application of biotechnologies in agriculture. This work aims to investigate Italian consumers' acceptance toward GM food. Through data collected from an original survey (N = 564), we tested a) their level of knowledge of GM techniques; b) if they are aware of differences between established techniques based on classical approaches of crossing and selection and more precise biotechnological techniques; c) their propensity to buy GM food, with a specific focus on food safety and environmental sustainability issues. By using a Multinomial Logit Model (MNL), starting from three hypotheses, the study highlights a gap in knowledge transfer and, in general, the communication process. This results in widespread misinformation that hinders informed consumer choices. The study also emphasizes consumers' sensitivity to food safety, including environmental issues, but still related to food safety issues.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"303-315"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-based directed energy deposition of Monel K-500 and Stellite 6 multi-materials 基于激光的定向能沉积 Monel K-500 和 Stellite 6 复合材料

IF 10.6 1区工程技术

Virtual and Physical Prototyping Pub Date : 2024-12-31 DOI: 10.1080/17452759.2024.2399789

Ze Chen, Zhongji Sun, Yang Qi, Wei Fan, Verner Soh Qun Liang, Sastry Yagnanna Kandukuri, Paulo Jorge Da Silva Bartolo, Kun Zhou

{"title":"Laser-based directed energy deposition of Monel K-500 and Stellite 6 multi-materials","authors":"Ze Chen, Zhongji Sun, Yang Qi, Wei Fan, Verner Soh Qun Liang, Sastry Yagnanna Kandukuri, Paulo Jorge Da Silva Bartolo, Kun Zhou","doi":"10.1080/17452759.2024.2399789","DOIUrl":"https://doi.org/10.1080/17452759.2024.2399789","url":null,"abstract":"Laser-based directed energy deposition (L-DED) offers significant advantages for repairing metal structures, particularly in the marine and offshore industry where corrosion-resistant materials like Monel K-500 (a Ni-Cu alloy) lack strength and wear resistance. This study addresses this issue by producing Monel K-500 with high-strength Stellite 6 (a Co-Cr alloy). Two types of multi-material samples were created using L-DED: interlayered and mixed powder samples. The interlayered samples experienced cracking at the material interface, while the mixed powder samples were crack-free and exhibited improved mechanical properties, with yield strength increasing from 208.3 MPa to 490.2 MPa and ultimate tensile strength from 429.7 MPa to 887.0 MPa compared to single Monel K-500 samples. This research demonstrated the potential of in-situ alloying during the L-DED process to enhance alloy properties and highlighted the challenges of abrupt compositional changes leading to cracking, suggesting mitigation strategies for successful production.","PeriodicalId":23756,"journal":{"name":"Virtual and Physical Prototyping","volume":"65 1","pages":""},"PeriodicalIF":10.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142250700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risk assessment of homologous variants of biotech trait proteins using a bridging approach. 采用桥接法对生物技术性状蛋白的同源变体进行风险评估。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-11-09 DOI: 10.1080/21645698.2024.2420412

Cunxi Wang, Jennifer Calcaterra, Beverly Anderson, Timothy Rydel, Rong Wang, Lieselot Bertho, Scott A Saracco, Kimberly Hodge-Bell, Luis Burzio, Tommi White, Bin Li

{"title":"Risk assessment of homologous variants of biotech trait proteins using a bridging approach.","authors":"Cunxi Wang, Jennifer Calcaterra, Beverly Anderson, Timothy Rydel, Rong Wang, Lieselot Bertho, Scott A Saracco, Kimberly Hodge-Bell, Luis Burzio, Tommi White, Bin Li","doi":"10.1080/21645698.2024.2420412","DOIUrl":"10.1080/21645698.2024.2420412","url":null,"abstract":"A transgenic protein is frequently expressed as different homologous variants in genetically modified crops due to differential processing of targeting peptides or optimization of activity and specificity. The aim of this study was to develop a science-based approach for risk assessment of homologous protein variants using dicamba mono-oxygenase (DMO) as a case study. In this study, DMO expressed in the next-generation dicamba-tolerant maize, sugar beet and soybean crops exhibited up to 27 amino acid sequence differences in the N-terminus. Structure modeling using AlphaFold, ESMFold and OpenFold demonstrates that these small N-terminal extensions lack an ordered secondary structure and do not disrupt the DMO functional structure. Three DMO variants were demonstrated to have equivalent immunoreactivity and functional activity ranging from 214 to 331 nmol/min/mg. Repeated toxicity studies using each DMO variant found no test substance-related adverse effects. These results support that homologous protein variants, which have demonstrated physicochemical and functional equivalence, can leverage existing safety data from one variant without requiring additional de novo safety assessments.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"336-351"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in the Application of Organoids-On-A-Chip in Diseases. 器官芯片在疾病中的应用进展。

IF 1.6 4区生物学

Organogenesis Pub Date : 2024-12-31 Epub Date: 2024-08-10 DOI: 10.1080/15476278.2024.2386727

Qiao Geng, Yanyan Xu, Yang Hu, Lu Wang, Yi Wang, Zhimin Fan, Desong Kong

引用次数: 0

Biotechnology executive order opens door for regulatory reform and social acceptance of genetically engineered microbes in agriculture. 生物技术行政命令为农业转基因微生物的监管改革和社会接受打开了大门。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-07-27 DOI: 10.1080/21645698.2024.2381294

Jabeen Ahmad, Amy Grunden, Jennifer Kuzma

引用次数: 0

Risk-appropriate regulations for gene-editing technologies. 基因编辑技术的风险适当监管。

IF 3.9 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-01-12 DOI: 10.1080/21645698.2023.2293510

Graham Brookes, Stuart J Smyth

引用次数: 0

Regulatory framework for genetically modified organisms in the Kingdom of Eswatini. 埃斯瓦提尼王国转基因生物监管框架。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-07-04 DOI: 10.1080/21645698.2024.2375664

Bongani Z Nkhabindze, Cebisile N Magagula, Diana Earnshaw, Calsile F Mhlanga, Sipho N Matsebula, Isaac G Dladla

引用次数: 0

Effects of path patterns on residual stresses and deformations of directed energy deposition-arc built blocks 路径模式对定向能沉积弧形构件残余应力和变形的影响

IF 10.6 1区工程技术

Virtual and Physical Prototyping Pub Date : 2024-12-31 DOI: 10.1080/17452759.2023.2296128

Donghong Ding, Rong Huang, Tao Liu, Lei Yuan, Chuan Liu

引用次数: 0

Twenty-eight years of GM Food and feed without harm: why not accept them? 转基因食品和饲料二十八年无危害：为什么不接受它们？

IF 3.9 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2024-12-31 Epub Date: 2024-03-12 DOI: 10.1080/21645698.2024.2305944

Richard E Goodman

{"title":"Twenty-eight years of GM Food and feed without harm: why not accept them?","authors":"Richard E Goodman","doi":"10.1080/21645698.2024.2305944","DOIUrl":"10.1080/21645698.2024.2305944","url":null,"abstract":"Since the first genetically engineered or modified crops or organisms (GMO) were approved for commercial production in 1995, no new GMO has been proven to be a hazard or cause harm to human consumers. These modifications have improved crop efficiency, reduced losses to insect pests, reduced losses to viral and microbial plant pathogens and improved drought tolerance. A few have focused on nutritional improvements producing beta carotene in Golden Rice. Regulators in the United States and countries signing the CODEX Alimentarius and Cartagena Biosafety agreements have evaluated human and animal food safety considering potential risks of allergenicity, toxicity, nutritional and anti-nutritional risks. They consider risks for non-target organisms and the environment. There are no cases where post-market surveillance has uncovered harm to consumers or the environment including potential transfer of DNA from the GMO to non-target organisms. In fact, many GMOs have helped improve production, yield and reduced risks from chemical insecticides or fungicides. Yet there are generic calls to label foods containing any genetic modification as a GMO and refusing to allow GM events to be labeled as organic. Many African countries have accepted the Cartagena Protocol as a tool to keep GM events out of their countries while facing food insecurity. The rationale for those restrictions are not rational. Other issues related to genetic diversity, seed production and environmental safety must be addressed. What can be done to increase acceptance of safe and nutritious foods as the population increases, land for cultivation is reduced and energy costs soar?","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"40-50"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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