{"title":"Protocol-Dependent Morphological Changes in Human Embryonic Stem Cell Aggregates during Differentiation toward Early Pancreatic Fate.","authors":"Elmira Rezaei Zonooz, Zahra Ghezelayagh, Azadeh Moradmand, Hossein Baharvand, Yaser Tahamtani","doi":"10.1159/000527863","DOIUrl":"10.1159/000527863","url":null,"abstract":"<p><p>Cell therapy is one of the promising approaches used against type 1 diabetes. Efficient generation of human embryonic stem cell (hESC)-derived pancreatic progenitors (PPs) is of great importance. Since signaling pathways underlying human pancreas development are not yet fully understood, various differentiation protocols are conducted, each considering variable duration, timing, and concentrations of growth factors and small molecules. Therefore, we compared two PP differentiation protocols in static suspension culture. We tested modified protocols developed by Pagliuca et al. (protocol 1) and Royan researchers (protocol 2) until early PP stage. The morphological changes of hESC aggregates during differentiation, and also gene and protein expression after differentiation, were evaluated. Different morphological structures were formed in each protocol. Quantitative gene expression analysis, flow cytometry, and immunostaining revealed a high level of PDX1 expression on day 13 of Royan's differentiation protocol compared to protocol 1. Our data showed that using protocol 2, cells were further differentiated until day 16, showing higher efficiency of early PPs. Moreover, protocol 2 is able to produce hESCs-PPs in a static suspension culture. Since protocol 2 is inexpensive in terms of media, growth factors, and chemicals, it can be used for massive production of PPs using static and dynamic suspension cultures.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40465157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cells Tissues OrgansPub Date : 2024-01-01Epub Date: 2023-04-27DOI: 10.1159/000529974
Göksel Doğan, Nedim Karagenç, Kerem Esmen, Bengi Çınar Kul, Hasan Yeşilkaya, Şakir Akgün, Mehmet Nurullah Orman, Mustafa Sandıkçı, Ülker Eren, Hümeyra Ünsal, Levent Karagenç
{"title":"Expression of Toll-Like Receptors in the Lung Tissue of Mouse Fetuses Generated by in vitro Embryo Culture and Embryo Transfer.","authors":"Göksel Doğan, Nedim Karagenç, Kerem Esmen, Bengi Çınar Kul, Hasan Yeşilkaya, Şakir Akgün, Mehmet Nurullah Orman, Mustafa Sandıkçı, Ülker Eren, Hümeyra Ünsal, Levent Karagenç","doi":"10.1159/000529974","DOIUrl":"10.1159/000529974","url":null,"abstract":"<p><p>Mouse fetuses generated by in vitro embryo culture and embryo transfer exhibit impaired lung development, altered composition of pulmonary epithelial cells associated with downregulation of several genes involved in lung development and toll-like receptor (TLR) signaling pathway. The aims of the present study were to determine the expression of all TLRs and to examine if the expression of TLRs, along with genes involved in TLR signaling pathway, is altered in the lung tissue of mouse fetuses generated through embryo culture and embryo transfer. Two experimental (EGs) and one control (CG) group were included in the study. Embryos cultured at 5% CO2-95% air for 95 h or less than 24 h were transferred to pseudo-pregnant females to obtain fetuses comprising EGin vitro (n = 18) and EGin vivo (n = 18), respectively. Fetuses obtained from naturally ovulating females on day 18 of pregnancy served as the CG (n = 18). Western blot and immunohistochemistry were used to determine the expression of TLR proteins. The expression of transcripts encoding TLRs, and the genes involved in TLR signaling pathway (Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos), was determined using qRT-PCR. While all TLRs were expressed by cells lining the bronchial/bronchiolar epithelium of lung tissues in all groups, some of the TLRs were expressed in a specific pattern. When compared to CG, the expression of transcripts encoding TLR-2, -3, -4, -5, -7, -8, -9, -12, -13, Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos was significantly downregulated in both EGs. It appears that stress imposed on embryos at preimplantation stages of development is associated with downregulation of TLRs, along with some of the genes involved in TLR signaling pathway, in the lung tissue during the perinatal period. It remains to be determined if downregulation of TLRs, along with the genes involved in TLR signaling pathway, has any functional consequences in the adult lung tissue.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9726318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryan J. Gilbert, NY Usa Gujon Troy, Sheng, Japan Christoph Kumamoto, Göttingen Germany Stefan Viebahn, Tübingen Germany Kacey G Liebau, Marra, S. Yip, N. Wang, R. Sugimura, Hong Kong
{"title":"Prelims","authors":"Ryan J. Gilbert, NY Usa Gujon Troy, Sheng, Japan Christoph Kumamoto, Göttingen Germany Stefan Viebahn, Tübingen Germany Kacey G Liebau, Marra, S. Yip, N. Wang, R. Sugimura, Hong Kong","doi":"10.1159/000534119","DOIUrl":"https://doi.org/10.1159/000534119","url":null,"abstract":"","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138618353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R.A.D. Kamath, M. D. Benson, Akhavan Rahnama, Soufi Zomorrod, S. K. W. Eghbalsaied, Isfahan, G. C. Baan, H. Maas, Amsterdam
{"title":"Contents 2023 Vol. 212","authors":"R.A.D. Kamath, M. D. Benson, Akhavan Rahnama, Soufi Zomorrod, S. K. W. Eghbalsaied, Isfahan, G. C. Baan, H. Maas, Amsterdam","doi":"10.1159/000535530","DOIUrl":"https://doi.org/10.1159/000535530","url":null,"abstract":"","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139025002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Front & Back Matter","authors":"R. Gilbert, Guojun Sheng, L. Bartolo, N. Vrana","doi":"10.1159/000533217","DOIUrl":"https://doi.org/10.1159/000533217","url":null,"abstract":"","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46537586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Gilbert, Guojun Sheng, George J. Christ, L. Bartolo, N. Vrana
{"title":"Front & Back Matter","authors":"R. Gilbert, Guojun Sheng, George J. Christ, L. Bartolo, N. Vrana","doi":"10.1159/000531363","DOIUrl":"https://doi.org/10.1159/000531363","url":null,"abstract":"","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43246692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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