Protective Effect of Vaccinium myrtillus Extract on X-Ray Irradiation-Induced Retinal Toxicity via eNOS and 8-OHdG expression.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2023-07-28 DOI:10.1159/000532011
Hüseyin Fındık, Mehmet Gökhan Aslan, Murat Okutucu, Adnan Yılmaz, Levent Tümkaya, Tolga Mercantepe, Kerimali Akyıldız, Feyzahan Uzun
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引用次数: 0

Abstract

Every year, hundreds of thousands of cancer patients receive radiotherapy treatment. Oxidative stress is observed in healthy tissues due to irradiation exposure. The present study is the first to address the effects of Vaccinium myrtillus (whortleberry, WB) against the effects of X-ray irradiation on retinal tissue. Twenty-four Sprague-Dawley rats were randomly allocated into 4 groups: (1) control group: rats without any treatment, (2) X-ray irradiation group: 8 Gray (Gy) RT for 2 days, (3) 100 mg WB extract + X-ray irradiation group: 8 Gy irradiation for 2 days and followed by intraperitoneal (IP) WB extract (100 mg/kg) supplementation for 10 days, (4) 200 mg WB extract + X-ray irradiation group: 8 Gy irradiation for 2 days and followed by IP WB extract (200 mg/kg) supplementation for 10 days. Eyes were enucleated on the 10th day after RT for histopathological, immunohistochemical (8-hydroxy-2'-deoxyguanosine [8-OHdG], endothelial nitric oxide synthase [eNOS]), and biochemical analyses (glutathione peroxidase [GSH], and malondialdehyde [MDA]). The GSH levels significantly decreased and MDA levels and 8-OHdG staining increased after X-ray irradiation compared to the control group. Combined X-ray irradiation + WB treatment significantly increased GSH levels and significantly decreased MDA production and 8-OHdG staining. However, eNOS staining was not affected in any of the groups. Besides, X-ray irradiation significantly increased cell losses and edematous areas. The WB significantly reversed the cellular damage in ganglion cells, inner nuclear, and outer nuclear layers in quantitative analyses. The X-ray irradiation caused significant retinal impairment, and additional WB therapy provided protective effects against radiation-induced retinopathy. These results may suggest WB extract as an adjuvant therapy to reverse retinal impairments after X-ray irradiation.

越桔提取物通过 eNOS 和 8-OHdG 表达对 X 射线照射诱导的视网膜毒性的保护作用
每年,数十万癌症患者接受放射治疗。在健康组织中也能观察到因照射而产生的氧化应激。本研究首次探讨了欧洲越橘(WB)对 X 射线照射视网膜组织的影响。24 只 Sprague-Dawley 大鼠被随机分为 4 组:(1) 对照组:未接受任何治疗的大鼠;(2) X 射线辐照组:(3) 100 毫克 WB 提取物 + X 射线照射组:(3) 100 毫克 WB 提取物 + X 射线照射组:8 Gy 照射 2 天,然后腹腔注射(IP)WB 提取物(100 毫克/千克)10 天;(4) 200 毫克 WB 提取物 + X 射线照射组:8 Gy 照射 2 天,然后腹腔注射(IP)WB 提取物(100 毫克/千克)10 天:8 Gy 照射 2 天,然后补充 IP WB 提取物(200 毫克/千克)10 天。辐照后第 10 天,眼球去核,进行组织病理学、免疫组化(8-羟基-2'-脱氧鸟苷 [8-OHdG]、内皮一氧化氮合酶 [eNOS])和生化分析(谷胱甘肽过氧化物酶 [GSH] 和丙二醛 [MDA])。与对照组相比,X射线照射后GSH水平明显下降,MDA水平和8-OHdG染色增加。X射线照射+WB联合治疗可明显提高GSH水平,明显降低MDA生成和8-OHdG染色。然而,eNOS染色在所有组别中均未受到影响。此外,X 射线照射明显增加了细胞损失和水肿面积。在定量分析中,WB 能明显逆转神经节细胞、核内层和核外层的细胞损伤。X 射线照射导致视网膜明显受损,而额外的 WB 治疗对辐射诱导的视网膜病变具有保护作用。这些结果表明 WB 提取物可作为一种辅助疗法来逆转 X 射线照射后的视网膜损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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