Cells Tissues Organs最新文献_第5页

Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid. 培养中的肌母细胞分化：关注血清和 GABA。

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-03-03 DOI: 10.1159/000529839

Guzel Sibgatullina, Rahaf Al Ebrahim, Karina Gilizhdinova, Anna Tokmakova, Artem Malomouzh

{"title":"Differentiation of Myoblasts in Culture: Focus on Serum and Gamma-Aminobutyric Acid.","authors":"Guzel Sibgatullina, Rahaf Al Ebrahim, Karina Gilizhdinova, Anna Tokmakova, Artem Malomouzh","doi":"10.1159/000529839","DOIUrl":"10.1159/000529839","url":null,"abstract":"There are many facts about the possible role of gamma-aminobutyric acid (GABA) in the development and differentiation of cells not only in nervous but also in muscle tissue. In the present study, a primary culture of rat skeletal muscle myocytes was used to evaluate the correlation between the content of GABA in the cytoplasm and the processes of myocyte division and their fusion into myotubes. The effect of exogenous GABA on the processes of culture development was also estimated. Since the classical protocol for working with myocyte cultures involves the use of fetal bovine serum (FBS) to stimulate cell division (growth medium) and horse serum (HS) to activate the differentiation process (differentiation medium), the studies were carried out both in the medium with FBS and with HS. It was found that cells grown in medium supplemented with FBS contain more GABA compared to cultures growing in medium supplemented with HS. Addition of exogeneous GABA leads to a decrease in the number of myotubes formed in both media, while the addition of an amino acid to the medium supplemented with HS had a more pronounced inhibitory effect. Thus, we have obtained data indicating that GABA is able to participate in the early stages of skeletal muscle myogenesis by modulating the fusion process.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9083148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum. 勘误。

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2024-04-15 DOI: 10.1159/000538569

引用次数: 0

Coupled Mutual Inhibition and Mutual Activation Motifs as Tools for Cell-Fate Control. 作为细胞命运控制工具的耦合相互抑制和相互激活图案

IF 2.9 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-02-09 DOI: 10.1159/000529558

Burhanuddin Sabuwala, Kishore Hari, Abhishek Shanmuga Vengatasalam, Mohit Kumar Jolly

{"title":"Coupled Mutual Inhibition and Mutual Activation Motifs as Tools for Cell-Fate Control.","authors":"Burhanuddin Sabuwala, Kishore Hari, Abhishek Shanmuga Vengatasalam, Mohit Kumar Jolly","doi":"10.1159/000529558","DOIUrl":"10.1159/000529558","url":null,"abstract":"Multistability is central to biological systems. It plays a crucial role in adaptation, evolvability, and differentiation. The presence of positive feedback loops can enable multistability. The simplest of such feedback loops are (a) a mutual inhibition (MI) loop, (b) a mutual activation (MA) loop, and (c) self-activation. While it is established that all three motifs can give rise to bistability, the characteristic differences in the bistability exhibited by each of these motifs is relatively less understood. Here, we use dynamical simulations across a large ensemble of parameter sets and initial conditions to study the bistability characteristics of these motifs. Furthermore, we investigate the utility of these motifs for achieving coordinated expression through cyclic and parallel coupling amongst them. Our analysis revealed that MI-based architectures offer discrete and robust control over gene expression, multistability, and coordinated expression among multiple genes, as compared to MA-based architectures. We then devised a combination of MI and MA architectures to improve coordination and multistability. Such designs help enhance our understanding of the control structures involved in robust cell-fate decisions and provide a way to achieve controlled decision-making in synthetic systems.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9252904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild Synovitis Impairs Chondrogenic Joint Environment. 轻度滑膜炎损害关节软骨环境

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-07-31 DOI: 10.1159/000532008

Sharada Paudel, Tyler Feltham, Lumanti Manandhar, Yi Guo, Lew Schon, Zijun Zhang

引用次数: 0

Emergence of Spatial Scales and Macroscopic Tissue Dynamics in Active Epithelial Monolayers. 活性上皮单层中空间尺度和宏观组织动力学的出现

IF 2.9 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-04-12 DOI: 10.1159/000528501

Padmalochini Selvamani, Raghunath Chelakkot, Amitabha Nandi, Mandar M Inamdar

{"title":"Emergence of Spatial Scales and Macroscopic Tissue Dynamics in Active Epithelial Monolayers.","authors":"Padmalochini Selvamani, Raghunath Chelakkot, Amitabha Nandi, Mandar M Inamdar","doi":"10.1159/000528501","DOIUrl":"10.1159/000528501","url":null,"abstract":"Migrating cells in tissues are often known to exhibit collective swirling movements. In this paper, we develop an active vertex model with polarity dynamics based on contact inhibition of locomotion (CIL). We show that under this dynamics, the cells form steady-state vortices in velocity, polarity, and cell stress with length scales that depend on polarity alignment rate (ζ), self-motility (v0), and cell-cell bond tension (λ). When the ratio λ/v0 becomes larger, the tissue reaches a near jamming state because of the inability of the cells to exchange their neighbors, and the length scale associated with tissue kinematics increases. A deeper examination of this jammed state provides insights into the mechanism of sustained swirl formation under CIL rule that is governed by the feedback between cell polarities and deformations. To gain additional understanding of how active forcing governed by CIL dynamics leads to large-scale tissue dynamics, we systematically coarse-grain cell stress, polarity, and motility and show that the tissue remains polar even on larger length scales. Overall, we explore the origin of swirling patterns during collective cell migration and obtain a connection between cell-level dynamics and large-scale cellular flow patterns observed in epithelial monolayers.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9295460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Variability in Cell and Nucleus Shapes. 关于细胞和细胞核形状的变化。

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2022-10-31 DOI: 10.1159/000527825

Anusha Devulapally, Varun Parekh, Clint Pazhayidam George, Sreenath Balakrishnan

{"title":"On the Variability in Cell and Nucleus Shapes.","authors":"Anusha Devulapally, Varun Parekh, Clint Pazhayidam George, Sreenath Balakrishnan","doi":"10.1159/000527825","DOIUrl":"10.1159/000527825","url":null,"abstract":"Cell morphology is an important regulator of cell function. Many abnormalities in cellular behavior can be discerned from changes in the shape of the cell and its organelles, typically the nucleus. Two major challenges for developing such phenotypic assays are reconstructing 3D surfaces of individual cells and nuclei from confocal images and developing characterizations of these surfaces for comparisons. We demonstrate two algorithms - 3D active contours and 3D condensed-attention UNet - to segment cells and nuclei from confocal images. The cell and nuclear surfaces are then converted into vectors using a reversible, spherical transform - i.e., shapes can be recovered from the vectors. Typical methods for characterizing shapes using size, shape, and image parameters such as area, volume, shape factor, solidity, and pixel intensities are not amenable to such reverse transformation. Our vector representation's principal component analysis shows that the significant modes of variability among cell and nucleus shapes are scaling and flattening. We benchmark these modes using a known mechanical model for nucleus morphology. Subsequent modes alter the eccentricity of the nucleus and translate and rotate it with respect to the cell. Our vector-space representation of cell and nucleus shape helps physically interpret the variability sources. It may further help to guide mechanical models and identify molecular mechanisms driving cell and nuclear shape changes.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40659483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocyte Growth Factor Promotes Differentiation Potential and Stress Response of Human Stem Cells from Apical Papilla. 肝细胞生长因子促进乳头尖部人类干细胞的分化潜能和应激反应

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2022-09-28 DOI: 10.1159/000527212

Zhenhai Liu, Na Yan, Ying Chen, Bin Hu

{"title":"Hepatocyte Growth Factor Promotes Differentiation Potential and Stress Response of Human Stem Cells from Apical Papilla.","authors":"Zhenhai Liu, Na Yan, Ying Chen, Bin Hu","doi":"10.1159/000527212","DOIUrl":"10.1159/000527212","url":null,"abstract":"Harsh local microenvironment, such as hypoxia and lack of instructive clues for transplanted stem cells, presents the serious obstacle for stem cell therapies' efficacy. Therefore, continued efforts have been taken to improve stem cells' viability and plasticity. Hepatocyte growth factor (HGF) has previously been reported to mitigate the complications of various human diseases in animal model studies and in some clinical trials. Besides, human stem cells from the root apical papilla (SCAP) are deemed a better resource of mesenchymal stem cells due to derived stem cells holding greater amplification ability in vitro compared with those from other dental resources. To move forward, evaluating effects and understanding underlying molecular mechanisms of HGF on SCAP for periodontal regeneration are needed. In this study, HGF was transgenically expressed in SCAP, and it was found that HGF enhanced osteo/dentinogenic differentiation capacity of SCAP compared with those of non-treated control in an ectopic mineralization model. Moreover, HGF reduced the apoptosis of SCAP under both normoxic and hypoxic conditions, whereas the combination of HGF and hypoxia exposure had inhibitory effects on cell proliferation during an 8-day in vitro culture period. Transcriptome analysis further revealed that suppressed cell cycle progression and activated BMP/TGFβ, Hedgehog, WNT, FGF, HOX, and other morphogen family members result upon HGF overexpression, which may render SCAP recapitulate part of neural crest stem cell characteristics. Moreover, strengthened stress response modulation such as unfolded protein response, macroautophagy, and anti-apoptotic molecules might explain the increased viability of SCAP. In all, our results imply that these potential mechanisms underlying HGF-promoting SCAP differentiation could be further elucidated and harnessed to improve periodontal tissue regeneration.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40381565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Momordica charantia Enhances Tendon Healing in Rats: An Experimental Study. Momordica charantia Enhances Tendon Healing in Rats：一项实验研究。

IF 2.9 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-08-16 DOI: 10.1159/000533644

Furkan Erdoğan, Arife Ahsen Kaplan, Hüseyin Sina Coşkun, Gamze Altun, Berrin Zuhal Altunkaynak, Ebru Kelsaka, Suleyman Kaplan, Ahmet Pişkin

{"title":"Momordica charantia Enhances Tendon Healing in Rats: An Experimental Study.","authors":"Furkan Erdoğan, Arife Ahsen Kaplan, Hüseyin Sina Coşkun, Gamze Altun, Berrin Zuhal Altunkaynak, Ebru Kelsaka, Suleyman Kaplan, Ahmet Pişkin","doi":"10.1159/000533644","DOIUrl":"10.1159/000533644","url":null,"abstract":"Momordica charantia (MC) is a traditional plant widely used since ancient times for wound healing. This study evaluated its potential effects on tendon healing. Adult male Wistar albino rats (n = 32, 8 rats in each group) were anesthetized, and their Achilles tendons were prepared for surgical procedures. Group 1 (Cont = control group) was not subjected to any surgery and was used as a control group for baseline values. Group 2 (PR = primary repair group) underwent primary repair (PR) with a monofilament suture after a full-thickness incision of the Achilles tendon. A full-thickness incision was also made to the Achilles tendon of group 3 (CT = collagen tube-administered group), followed by PR and collagen tube insertion. In group 4 (MC = M. charantia-administered group), 1 mL of MC extract was applied locally on the collagen tube in addition to the surgical procedure applied to group 3. The Achilles tendons were excised on the postoperative 40th day and examined stereologically, histologically, and bioinformatically. Data showed that the total volume of the collagen fibers was higher in MC and CT groups than in the PR group. The total volume of the tendon was decreased in MC and CT groups than in the Cont group. The ratios between the volumes of the collagen fibers and total tendon in the MC and CT groups were significantly different from PR, but not different from the Cont group. Additionally, MC improved tenoblastic activity, collagen production, and neovascularization. Bioinformatic interactions showed that the proteases of MC could trigger the signals playing a role on vasculogenesis, reducing inflammation, and contributing to tenoblast activation and collagen remodeling. MC extract ameliorates the healing of injured tendon and can provide satisfactory tendon repair. Further works are recommended to explore the healing capacity of MC.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10367404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life of the B10 Mouse: A View from the Hair Follicles and Tissue Stem Cells. B10 小鼠的生命：从毛囊和组织干细胞看小鼠的一生

IF 2.9 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2023-09-13 DOI: 10.1159/000533779

Kimihiko Sugaya

{"title":"Life of the B10 Mouse: A View from the Hair Follicles and Tissue Stem Cells.","authors":"Kimihiko Sugaya","doi":"10.1159/000533779","DOIUrl":"10.1159/000533779","url":null,"abstract":"In our series of studies, the changes in the skin characteristics of mice caused by aging were investigated in correlation with the stem cells for keratinocytes and melanocytes in the natural hair cycle until middle age. The aim of the present review was to investigate these characteristics of hair follicles (HFs) at older age and complete the analysis of these changes as a study throughout the mouse lifetime. In addition, stem cells for keratinocytes and melanocytes were evaluated for changes in skin characteristics caused by aging. Postnatal day 200 (P200) appears to be the age of complete maturation of skin and the onset of aging with regard to HFs. Keratin 15-positive keratinocyte stem cells complete their localization as a quantitatively sufficient amount of progenitor in the hair bulge region and orchestrate the regeneration of hairs in every anagen phase thereafter. Although their frequency is low, an unusual structure of HFs, curved HFs, appear for the first time at P200. Thereafter, abnormal hair curvature continues to increase throughout life. In contrast, HF characteristics derived from melanocytes begin to show a high frequency of hypopigmented hair bulbs at P200 and appear to lead to a significant increase in the number of white hairs. Curved HFs and white hairs were considered biomarkers of aging in mice. The number of tyrosinase-related protein 2-positive melanocyte stem cells in the hair bulge is extremely low and may be one cause underlying not only the induction of melanocyte-derived characteristics by aging but possibly also that of keratinocyte-derived characteristics. These results provide insight into the mechanisms of the actions of stem cells on hair regeneration through the aging process.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10234382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered Expression of Pulmonary Epithelial Cell Markers in Fetal and Adult Mice Generated by in vitro Embryo Culture and Embryo Transfer. 通过体外胚胎培养和胚胎移植产生的胎儿和成年小鼠肺上皮细胞标记物的表达改变。

IF 2.7 4区生物学

Cells Tissues Organs Pub Date : 2024-01-01 Epub Date: 2022-09-14 DOI: 10.1159/000527044

Göksel Doğan, Murat Öztürk, Didar Tuğçe Karakulak, Levent Karagenç

{"title":"Altered Expression of Pulmonary Epithelial Cell Markers in Fetal and Adult Mice Generated by in vitro Embryo Culture and Embryo Transfer.","authors":"Göksel Doğan, Murat Öztürk, Didar Tuğçe Karakulak, Levent Karagenç","doi":"10.1159/000527044","DOIUrl":"10.1159/000527044","url":null,"abstract":"Lung development is impaired in mice generated through transfer of in vitro-derived blastocysts. The main objective of the current study was to determine if the composition of epithelial cells in the fetal and adult lung tissue is altered in mice generated through transfer of in vitro-derived blastocysts. The study comprised two experimental (EGs) and two control (CGs) groups. Fetuses (18.5 d.p.c.) and adult mice (8 weeks old) of the EGs (EGfetus, n = 18; EGadult, n = 15) were produced by the transfer of day 5 F2 blastocysts to pseudo-pregnant females. F2 fetuses and adult mice derived from naturally ovulating females served as the CGs (CGfetus, n = 18; CGadult, n = 15). The expression of Tuba-1a (a marker of ciliated cells), Foxj-1 (a marker of motile ciliated cells), Uch-L1 (a marker of neuroendocrine cells), Cldn-10 (a marker of club cells), Aqp-5 (a marker of type I alveolar cells), and Sp-C (a marker of type II alveolar cells) was determined using Western blot, immunohistochemistry/immunofluorescence, and quantitative RT-PCR analyses. Weight of fetuses as well as adult mice is decreased in mice comprising the EGs. Impaired lung development observed in EGfetus was associated with altered expression of Tuba-1a, Foxj-1, Cldn-10, Uch-L1, Sp-C, and Aqp-5. Morphology of the adult lung tissue was similar between the groups except for a significant increase in the thickness of the epithelia in EGadult. The expression of Cldn-10 and Sp-C was also altered in EGadult. It remains to be determined whether altered expression of these genes has any long-term impact on epithelial cell functions in the adult lung tissue.","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40356924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0