Cells Tissues Organs最新文献

{"title":"Comparative Morphology and Immunohistochemistry of the Adipose Tissues at the Posteromedial Tibial Border and the Heel Fat Pad in the Human.","authors":"Takumi Okunuki, Masafumi Ohsako, Hirai Suito, Tomonobu Ishigaki, Toshihiro Maemichi, Hajime Ishida, Hiroki Yabiku, Chinatsu Azuma, Kotaro Nishida, Tsukasa Kumai","doi":"10.1159/000549193","DOIUrl":"10.1159/000549193","url":null,"abstract":"<p><strong>Introduction: </strong>A previous study confirmed the presence of adipose tissue along the posteromedial tibial border. This tissue may be the cause of medial shin pain, such as in medial tibial stress syndrome. However, the morphological and immunohistochemical characteristics of adipose tissue along the posteromedial tibial border remain unclear. In this study, we investigated the morphological and immunohistochemical characteristics of this tissue and compared them to those of the heel fat pad.</p><p><strong>Methods: </strong>Seven cadavers were fixed in 10% formalin solution, and specimens were sampled from the adipose tissue along the posteromedial tibial border and heel fat pad.</p><p><strong>Results: </strong>Scanning electron microscopy images showed a basket-like fibrous structure surrounding the adipocytes. This fibrous structure formed a three-dimensional network connecting adipocytes in both the posteromedial tibial border and the heel fat pad. The area and perimeter of the adipocytes were not significantly different between the adipose tissue along the posteromedial tibial border and the heel fat pads. Additionally, immunohistochemical staining confirmed that the fibres around the adipocytes were type I and III collagen fibres. These adipose tissues also reacted positively to substance P.</p><p><strong>Conclusion: </strong>The morphological and immunohistochemical characteristics of the adipose tissue along the posteromedial tibial border may be a structure adapted to carry mechanical stress. Additionally, the presence of substance P indicated that the adipose tissue along the posteromedial tibial border may be a source of pain, such as that experienced in medial tibial stress syndrome.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"205-215"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocytotic Albumin Uptake Pathways in Human Adipose Stem Cells and Connection to Intracellular Calcium Oscillations and the Neonatal Fc receptor.","authors":"Md Tanvir Morshed, Anne Bernhardt, Maria Wartenberg, Heinrich Sauer","doi":"10.1159/000545773","DOIUrl":"10.1159/000545773","url":null,"abstract":"<p><p><p>Introduction: Intracellular Ca2+ oscillations of unknown function occur in human adipose tissue stem cells (ASCs). In the present study, we investigated whether Ca2+ oscillations in ASCs from subcutaneous fat tissue derived from female patients were driving albumin endocytosis by involving the neonatal Fc receptor (FcRn), which is mediating the recycling of albumin and IgG.</p><p><strong>Methods: </strong>Intracellular Ca2+ oscillations were monitored by Fluo-4 microfluorometry. Uptake of fluorescence-labeled albumin was assessed by confocal laser scanning microscopy in the presence of endocytotic pathway inhibitors. FcRn was inactivated either by use of the antagonizing antibody nipocalimab or siRNA technology.</p><p><strong>Results: </strong>Endocytosis of albumin occurred by macropinocytosis (inhibition by cytochalasin D, wortmannin, and ethylisopropylamiloride [EIPA]), caveolae-dependent (inhibition by genistein and nystatin) and clathrin-mediated (inhibition by Dyngo-4a) pathways. In serum-free medium, Ca2+ oscillations were absent, but were induced by the addition of either fetal bovine serum, albumin, IgG or a stimulating antibody against FcRn. Consequently, FcRn expression was demonstrated in ASCs by Western blot analysis and immunohistochemistry, and colocalized with endocytosed albumin. Ca2+ oscillations were inhibited by the Ca2+ chelating agent BAPTA, the store-operated Ca2+ entry inhibitor SKF96365, the Ca2+-sensing receptor (CaSR) inhibitor NPS-2143, the macropinocytosis inhibitors cytochalasin D, wortmannin, and EIPA, the caveolae-dependent endocytosis inhibitors genistein and nystatin, and the clathrin-mediated endocytosis inhibitor Dyngo-4a. Uptake of fluorescence-labeled albumin was inhibited by agents interfering with Ca2+ oscillations and endocytosis blockers. Notably, not only intracellular albumin and IgG accumulation, but also Ca2+ oscillations were inhibited by the FcRn-blocking antibody nipocalimab, which interferes with the IgG binding site of FcRn. Moreover, siRNA-mediated downregulation of FcRn protein expression significantly reduced intracellular albumin content, the number of cells displaying Ca2+ oscillations, and the duration and amplitude of Ca2+ signals.</p><p><strong>Conclusion: </strong>Our data suggest that Ca2+ oscillations in human ASCs regulate albumin uptake and presumably IgG recycling via FcRn. </p>.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"117-136"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12140596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of Signal Transducer and Activator of Transcription 4 Contributes to Impaired Osteogenic Differentiation of Human Bone Marrow Stem Cells during in vitro Expansion.","authors":"Weiqiong Rong, Yuanying Yuan, Shaomian Yao","doi":"10.1159/000544952","DOIUrl":"10.1159/000544952","url":null,"abstract":"<p><strong>Introduction: </strong>In vitro expansion of primary human bone marrow stem cells (hBMSCs) is necessary to obtain sufficient cells for therapeutic uses. Unfortunately, hBMSCs rapidly lose their osteogenic differentiation potential during expansion, significantly limiting their applications. Signal transducer and activator of transcription 4 (STAT4) is known to play roles in cell migration, proliferation, and differentiation. This study aimed to determine the expression and the role of STAT4 during the expansion of hBMSCs.</p><p><strong>Methods: </strong>STAT4 expression in different passages of hBMSCs was evaluated using qRT-PCR and Western blotting. RNA interference and adeno-associated virus serotype 2-mediated gene overexpression were employed to assess the function of STAT4. RNA samples from STAT4-overexpressing hBMSCs were analyzed by RNA-seq to identify differentially expressed genes (DEGs), followed by bioinformatics analyses to determine the pathways affected by STAT4.</p><p><strong>Results: </strong>STAT4 expression progressively decreases during the in vitro expansion of hBMSCs, concomitant with the loss of osteogenic differentiation potential. STAT4 knockdown in early passage hBMSCs significantly inhibits their osteogenic differentiation, evidenced by markedly reduced calcium deposition and downregulation of osteogenic markers. STAT4 knockdown also reduces hBMSCs' proliferation ability. Conversely, STAT4 overexpression notably increases calcium deposition in passage 3 to passage 7 cells, suggesting that enhanced STAT4 expression can mitigate the loss of osteogenic potential during hBMSC expansion. Transcriptomic analysis revealed DEGs in STAT4-overexpressing hBMSCs. Subsequent bioinformatics analyses indicated that some of these DEGs are involved in pathways regulating cell differentiation and senescence.</p><p><strong>Conclusion: </strong>The in vitro expansion of hBMSCs leads to the downregulation of STAT4, which contributes to the impairment of their osteogenic potential and may affect cell self-renewability. This study provides insight into the molecular mechanisms underlying the loss of osteogenic differentiation during hBMSC expansion and identifies STAT4 as a potential target for hBMSC-based bone regeneration therapies.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"27-41"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12353826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Biomaterials in Enhancing Cell Therapy.","authors":"Roaa Hadi, Justin Putman, Jorge Almodovar","doi":"10.1159/000551247","DOIUrl":"10.1159/000551247","url":null,"abstract":"<p><strong>Background: </strong>Cell-based therapies are revolutionizing medicine by offering regenerative and immunomodulatory capabilities beyond traditional treatments. These therapies hold promise for diseases, such as cancer, autoimmune disorders, and diabetes. However, clinical translation is challenged by immune rejection, reduced cell viability, and poor control over therapeutic delivery.</p><p><strong>Summary: </strong>Biomaterials can provide innovative solutions to these barriers by creating supportive environments, enhancing cell survival, and enabling targeted, sustained delivery. This review highlights advances in biomaterial strategies - including lipid and polymeric nanoparticles, hydrogels, fibrous scaffolds, and layer-by-layer assemblies - and their application across T-cell, macrophage, stem cells, and islet cell therapies. Each material class offers unique physicochemical and/or mechanical properties that can be tuned to meet the specific needs of different cell types and therapeutic contexts.</p><p><strong>Key messages: </strong>Biomaterials provide critical tools for enhancing the efficacy and precision of cell-based therapies. Despite substantial progress, challenges remain with selecting the appropriate biomaterial for specific applications and retaining biocompatibility long term. The ongoing development of patient-specific and adaptable biomaterials holds promise for further breakthroughs in regenerative medicine. This review underscores the potential of biomaterials to drive forward the field of cell therapy, opening new avenues for treating a wide range of diseases.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"306-322"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147303059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes Derived from Antler Stem Cell Promote Regenerative Burn Healing by the Facilitation of Angiogenesis and the Polarization of M2 Macrophages.","authors":"Jianwei Zhao, Jue Zhou, Xunsheng Li, Jinpeng Lv, Yidi Jiang, Yimin Wang, Hang Zhang, Bo Cui, Hongmei Sun","doi":"10.1159/000549520","DOIUrl":"10.1159/000549520","url":null,"abstract":"<p><strong>Introduction: </strong>The underlying mechanisms by which these exosomes facilitate healing in deep burns remain unclear. This study aimed to investigate the effect of antler stem cell (AnSC)-derived exosomes (AnSC-exos) on burn wound healing and to provide new insights for the clinical treatment of deep burn wounds.</p><p><strong>Methods: </strong>AnSC-exos were isolated through ultracentrifugation and subsequently administered to rat models with severe third-degree burn injuries. Wound closure rates, histological analysis, immunohistochemistry results and quantitative real-time PCR analysis were performed. In vitro, we assessed the effects of AnSC-exos on the proliferation and migration of human umbilical vein endothelial cells (HUVECs).</p><p><strong>Results: </strong>Our results convincingly demonstrate that AnSC-exos significantly enhance the speed and quality of healing in burn wounds. At 28 days post-burn injury, we found elevated levels of CD31 and CD163 in the healing tissues, accompanied by a reduction in iNOS levels. Additionally, the relative mRNA expression levels of ColA2/Col3A1 and TGF-β1 were significantly lower than the AnSC-exos group compared to the control group (p < 0.001, p < 0.0001), while the relative mRNA levels of MMP3 were significantly higher (p < 0.05). In vitro, AnSC-exos promotes the proliferation and migration of HUVECs.</p><p><strong>Conclusion: </strong>These results suggest that AnSC-exos facilitate burn wound repair by modulating M2 macrophage polarization, promoting angiogenesis, inhibiting myofibroblast production, and enhancing collagen deposition.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"230-243"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Temperature-Dependent Endocytic Regulation of Notch Signaling in Chick and Mouse Neural Progenitors.","authors":"Kai-Wei Lin, Pei-Rong Wu, Cheng-Chang Chen, Alexander Halim, Bo-Kai Liao, Pei-Shan Hou","doi":"10.1159/000551895","DOIUrl":"10.1159/000551895","url":null,"abstract":"<p><strong>Introduction: </strong>Temperature plays a crucial role in embryonic development, particularly in oviparous species that experience natural fluctuations during incubation. Unlike viviparous embryos developing under stable maternal conditions, chick embryos are exposed to external temperature variability that can influence cellular regulatory processes. Notch signaling, a conserved pathway essential for maintaining the neural progenitor cell (NPC) pool, is regulated by endosomal recycling; however, the mechanism by which temperature modulates this process across species remains poorly understood.</p><p><strong>Methods: </strong>To model environmental challenges experienced by chick embryos, we examined the effects of a physiologically relevant low temperature (30°C) on Dll1-mediated Notch signaling in chick and mouse NPCs.</p><p><strong>Results: </strong>Under hypothermic conditions, Dll1 reporter signals increased in chick NPCs but decreased in mouse NPCs, correlating with corresponding changes in Notch activity. Chick NPCs maintained progenitor gene expression at low temperature, whereas mouse NPCs showed reduced expression. Mechanistic analyses revealed that distinct recycling pathways are differentially required for Notch signaling across temperatures and species. Chick NPCs maintained Notch signaling through both fast recycling (FR) and slow recycling (SR) under normothermia and additional degradation under hypothermia, whereas mouse NPCs relied on SR and degradation under normothermia and showed impaired recycling under hypothermia.</p><p><strong>Conclusion: </strong>Together, these findings reveal species-specific adaptations in the temperature-dependent endocytic regulation of Notch signaling, highlighting how environmental temperature influences the maintenance of neural progenitors across species.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"284-305"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147632517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid Binding Peptide Regulates Extracellular Matrix Deposition and Diminishes Fibroblast Contractility.","authors":"Beth Blake, Whitney Ann Ponwith, Klaus Rischka, Martin Wiesing, Tugba Ozdemir","doi":"10.1159/000544881","DOIUrl":"10.1159/000544881","url":null,"abstract":"<p><strong>Introduction: </strong>Fibroblasts are central to a variety of homeostatic events such as wound healing and tissue regeneration. However, their pathologic activation is thought to play roles in a variety of diseases not only limited to fibrosis, foreign body reaction, scleroderma but also cancer metastasis. Biophysical properties of the extracellular matrix (ECM) deposited by an activated fibroblast determine whether there is a pro-regenerative or scarring response. Compared to aged fibroblasts, embryonic fibroblasts were shown to deposit a pro-regenerative ECM characterized by early hyaluronic acid (HA) deposition and increased levels of pro-regenerative collagens such as type III collagen. Since HA is also a regulator of collagen organization, we propose that early accumulation of HA by fibroblasts can facilitate pro-regenerative matrix formation. Given that the molecular weights of HA present in pro-regenerative matrix are higher than synthetic HA, we strategize attracting HA synthesized by fibroblasts. In this study, we used a synthetic peptide sequence known to have affinity to HA as a strategy to instruct fibroblasts to retain HA on the surface. We hypothesized that hyaluronic acid binding peptide (HABP) may instruct fibroblast endogenous HA deposition onto functionalized surfaces.</p><p><strong>Methods: </strong>We functionalized silica glass surfaces with HABP using aminoorganosilane mediated chemisorption and screened primary human dermal fibroblasts (HDFs) for cell morphology, cytoskeletal arrangement, and alpha-smooth muscle actin (α-SMA) expression.</p><p><strong>Results: </strong>Our results show HABP treated surfaces retain higher levels of HA on silica glass compared to control surfaces on fibroblast-derived matrices. Analysis of α-SMA shows increased α-SMA expression on hDFs and increased stress fiber formation. HABP treated surfaces were found to have reduced α-SMA expression. The physical features of collagen fibers deposited by fibroblasts were also organized differently in the presence of HABP.</p><p><strong>Conclusion: </strong>Due to their ability to diminish fibroblast contractility and promote regenerative ECM production, HABPs are a potentially viable strategy to instruct pro-regenerative fibroblasts and can be used therapeutically to treat fibrotic diseases.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"42-55"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory Response to Menstrual Fluid Does Not Induce Fibrotic Morphogenesis Program in Human Endometrial Stromal Cells.","authors":"Roman Eremichev, Mikhail Khandokhin, Natalya Alexandrushkina, Arina Radnaeva, Peter Nimiritsky, Vsevolod Tkachuk, Pavel Makarevich","doi":"10.1159/000550511","DOIUrl":"10.1159/000550511","url":null,"abstract":"<p><strong>Introduction: </strong>Human endometrium is one of peculiar tissues capable of scarless regeneration after injury during every menstrual cycle, birth, or surgery. However, it is disputable whether this feature should be attributed to specific regulatory factors of wound environment and menstrual fluid (MF) or to tissue-specific properties of endometrial mesenchymal stromal cells (eMSCs), which are pivotal participants of wound healing. We aimed to elucidate the role of eMSC tissue specificity in wound healing.</p><p><strong>Methods: </strong>We evaluated changes of eMSC transcriptomic profile in response to MF and their potency to granulation tissue formation in vitro in comparison with those of stromal cells from scar-forming organs - dermal and adipose MSC (dMSC and adMSC).</p><p><strong>Results: </strong>We have found that MF contains numerous inflammatory factors and induces a profound inflammatory response in both eMSC and dMSC, but a tissue-specific component was identified in their transcriptome profiles. Furthermore, transcriptomic tissue specificity was stable and present prior to MF treatment as well as after it, so we validated our findings against in vivo single-cell RNA-sequencing data from Human Protein Atlas. Tissue specificity traits were related to embryonic development and morphogenesis, suggesting a putative contribution of \"developmental imprinting\" in its establishment. Using in vitro models of fibroplasia, angiogenesis, and extracellular matrix deposition, we showed that eMSC lack the ability to induce these processes in contrast to dMSC and adMSC. Finally, we refined transcription factors (TFs) from stable tissue-specific genes that may explain the unique properties of eMSC and endometrium itself and can serve as potential targets to induce regeneration in scar-forming organs.</p><p><strong>Conclusion: </strong>eMSCs possess tissue-specific properties including stable expression of TFs that may explain the scarless regeneration of endometrium.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"271-283"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Statement.","authors":"","doi":"10.1159/000549820","DOIUrl":"10.1159/000549820","url":null,"abstract":"<p><p>The article \"Bathysa cuspidata Extract Modulates the Morphological Reorganization of the Scar Tissue and Accelerates Skin Wound Healing in Rats: A Time-Dependent Study\" [Cells Tissues Organs. 2015; 199(4):266-277. https://doi.org/10.1159/000365504] by Reggiani V. Gonçalves, Rômulo D. Novaes, Marli C. Cupertino, Bruna M. Araújo, Emerson F. Vilela, Aline T. Machado, João P.V. Leite and Sérgio L.P. Matta has been retracted by the Publisher and the Editors.After the publication of this article, concerns were raised about the integrity of some of the data presented. Specifically, 4 panels in Figure 5 of this article are the same as 4 panels of Figure 1 of a subsequently published article by the same author group, representing different treatment groups, with image rotation and cropping in some instances, [1]. In addition, 2 panels of Figure 5 of this article are the same as 2 panels of Figure 4 of a previously published paper by the authors, representing different treatment groups, with image rotation [2].When asked to comment, the authors responded to the above concerns and stated that these errors occurred during the formatting of the figures due to the use of previously published figures as templates during training. Editorial review concluded that the errors highlighted issues in the data management and, consequently, the conclusions based on that data may not be reliable, and therefore, this article is being retracted.The authors have not responded to our correspondence regarding this retraction despite multiple attempts of contact.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"81-82"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145942270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Media Comparisons for the Differentiation of Human Induced Pluripotent Stem Cells into the Otic Lineage.","authors":"Stefanie Klingenstein, Nils Hollweg, Stefan Liebau, Moritz Klingenstein","doi":"10.1159/000550296","DOIUrl":"10.1159/000550296","url":null,"abstract":"<p><strong>Introduction: </strong>Otic organoids differentiated from human pluripotent stem cells are three-dimensional in vitro cultures that broadly mimic the complexity and functionality of the human inner ear. They provide a valuable model for developmental and disease-related studies. However, current protocols differ substantially in efficiency and reproducibility. In this study, we investigated whether different stem cell maintenance media influence the differentiation of keratinocyte-derived induced pluripotent stem cells (kiPSCs) into the otic lineage.</p><p><strong>Methods: </strong>kiPSCs were cultured in either a self-made FTDA medium or the commercially available PeproGrow™ human embryonic stem cell medium and subsequently subjected to an established otic differentiation protocol. Early developmental stages, including the pre-placodal region, the otic placode, and pro-neural sensory regions, were analyzed using immunofluorescence and gene expression profiling.</p><p><strong>Results: </strong>While no significant differences were observed in iPSC maintenance or pluripotency between the two media, distinct differences emerged during otic differentiation. Media composition influenced the expression of placodal, otic, and pro-sensory markers at multiple stages, indicating differential responsiveness to otic induction cues.</p><p><strong>Conclusion: </strong>Our findings demonstrate that stem cell maintenance media composition is a critical determinant of subsequent otic lineage differentiation. These results provide guidance for optimizing stem cell culture conditions and improving the reproducibility of otic organoid differentiation protocols.</p>","PeriodicalId":9717,"journal":{"name":"Cells Tissues Organs","volume":" ","pages":"254-270"},"PeriodicalIF":2.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145948294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}