Cell calcium最新文献

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Calreticulin regulates hepatic stellate cell activation through modulating TGF-beta-induced Smad signaling 钙网蛋白通过调节 TGF-beta 诱导的 Smad 信号调节肝星状细胞活化
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-30 DOI: 10.1016/j.ceca.2024.102895
Chien-Chih Chen , Li-Wen Hsu , Kuang-Den Chen , King-Wah Chiu , Chao-Pin Kung , Shu-Rong Li , Chao-Long Chen , Kuang-Tzu Huang
{"title":"Calreticulin regulates hepatic stellate cell activation through modulating TGF-beta-induced Smad signaling","authors":"Chien-Chih Chen ,&nbsp;Li-Wen Hsu ,&nbsp;Kuang-Den Chen ,&nbsp;King-Wah Chiu ,&nbsp;Chao-Pin Kung ,&nbsp;Shu-Rong Li ,&nbsp;Chao-Long Chen ,&nbsp;Kuang-Tzu Huang","doi":"10.1016/j.ceca.2024.102895","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102895","url":null,"abstract":"<div><p>Liver fibrosis is characterized by excessive deposition of extracellular matrix (ECM) as a wound healing process. Activated hepatic stellate cells (HpSCs) are the major producer of the ECM and play a central role in liver fibrogenesis. It has been widely accepted that elimination of activated HpSCs or reversion to a quiescent state can be a feasible strategy for resolving the disease, further highlighting the urgent need for novel therapeutic targets. Calreticulin (CRT) is a molecular chaperone that normally resides in the endoplasmic reticulum (ER), important in protein folding and trafficking through the secretory pathway. CRT also plays a critical role in calcium (Ca<sup>2+</sup>) homeostasis, with its Ca<sup>2+</sup> storage capacity. In the current study, we aimed to demonstrate its function in directing HpSC activation. In a mouse liver injury model, CRT was up-regulated in HpSCs. In cellular experiments, we further showed that this activation was through modulating the canonical TGF-β signaling. As down-regulation of CRT in HpSCs elevated intracellular Ca<sup>2+</sup> levels through a form of Ca<sup>2+</sup> influx, named store-operated Ca<sup>2+</sup> entry (SOCE), we examined whether moderating SOCE affected TGF-β signaling. Interestingly, blocking SOCE had little effect on TGF-β-induced gene expression. In contrast, inhibition of ER Ca<sup>2+</sup> release using the inositol trisphosphate receptor inhibitor 2-APB increased TGF-β signaling. Treatment with 2-APB did not alter SOCE but decreased intracellular Ca<sup>2+</sup> at the basal level. Indeed, adjusting Ca<sup>2+</sup> concentrations by EGTA or BAPTA-AM chelation further enhanced TGF-β-induced signaling. Our results suggest a crucial role of CRT in the liver fibrogenic process through modulating Ca<sup>2+</sup> concentrations and TGF-β signaling in HpSCs, which may provide new information and help advance the current discoveries for liver fibrosis.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"121 ","pages":"Article 102895"},"PeriodicalIF":4.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140824431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Orai1/STIMs modulators in pulmonary vascular diseases 肺血管疾病中的 Orai1/STIMs 调节剂
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-24 DOI: 10.1016/j.ceca.2024.102892
Anaïs Saint-Martin Willer , David Montani , Véronique Capuano , Fabrice Antigny
{"title":"Orai1/STIMs modulators in pulmonary vascular diseases","authors":"Anaïs Saint-Martin Willer ,&nbsp;David Montani ,&nbsp;Véronique Capuano ,&nbsp;Fabrice Antigny","doi":"10.1016/j.ceca.2024.102892","DOIUrl":"10.1016/j.ceca.2024.102892","url":null,"abstract":"<div><p>Calcium (Ca<sup>2+</sup>) is a secondary messenger that regulates various cellular processes. However, Ca<sup>2+</sup> mishandling could lead to pathological conditions. Orai1 is a Ca<sup>2+</sup>channel contributing to the store-operated calcium entry (SOCE) and plays a critical role in Ca<sup>2+</sup> homeostasis in several cell types. Dysregulation of Orai1 contributed to severe combined immune deficiency syndrome, some cancers, pulmonary arterial hypertension (PAH), and other cardiorespiratory diseases. During its activation process, Orai1 is mainly regulated by stromal interacting molecule (STIM) proteins, especially STIM1; however, many other regulatory partners have also been recently described. Increasing knowledge about these regulatory partners provides a better view of the downstream signalling pathways of SOCE and offers an excellent opportunity to decipher Orai1 dysregulation in these diseases. These proteins participate in other cellular functions, making them attractive therapeutic targets. This review mainly focuses on Orai1 regulatory partners in the physiological and pathological conditions of the pulmonary circulation and inflammation.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"121 ","pages":"Article 102892"},"PeriodicalIF":4.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000502/pdfft?md5=aaccf561946fb9b4e10e4964cedbd2af&pid=1-s2.0-S0143416024000502-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140760190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A deep learning-based approach for efficient detection and classification of local Ca²⁺ release events in Full-Frame confocal imaging 基于深度学习的全帧共焦成像中局部 Ca²⁺ 释放事件的高效检测和分类方法
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-24 DOI: 10.1016/j.ceca.2024.102893
Prisca Dotti , Miguel Fernandez-Tenorio , Radoslav Janicek , Pablo Márquez-Neila , Marcel Wullschleger , Raphael Sznitman , Marcel Egger
{"title":"A deep learning-based approach for efficient detection and classification of local Ca²⁺ release events in Full-Frame confocal imaging","authors":"Prisca Dotti ,&nbsp;Miguel Fernandez-Tenorio ,&nbsp;Radoslav Janicek ,&nbsp;Pablo Márquez-Neila ,&nbsp;Marcel Wullschleger ,&nbsp;Raphael Sznitman ,&nbsp;Marcel Egger","doi":"10.1016/j.ceca.2024.102893","DOIUrl":"10.1016/j.ceca.2024.102893","url":null,"abstract":"<div><p>The release of Ca<sup>2+</sup> ions from intracellular stores plays a crucial role in many cellular processes, acting as a secondary messenger in various cell types, including cardiomyocytes, smooth muscle cells, hepatocytes, and many others. Detecting and classifying associated local Ca<sup>2+</sup> release events is particularly important, as these events provide insight into the mechanisms, interplay, and interdependencies of local Ca<sup>2+</sup>release events underlying global intracellular Ca<sup>2+</sup>signaling. However, time-consuming and labor-intensive procedures often complicate analysis, especially with low signal-to-noise ratio imaging data.</p><p>Here, we present an innovative deep learning-based approach for automatically detecting and classifying local Ca<sup>2+</sup> release events. This approach is exemplified with rapid full-frame confocal imaging data recorded in isolated cardiomyocytes.</p><p>To demonstrate the robustness and accuracy of our method, we first use conventional evaluation methods by comparing the intersection between manual annotations and the segmentation of Ca<sup>2+</sup> release events provided by the deep learning method, as well as the annotated and recognized instances of individual events. In addition to these methods, we compare the performance of the proposed model with the annotation of six experts in the field. Our model can recognize more than 75 % of the annotated Ca<sup>2+</sup> release events and correctly classify more than 75 %. A key result was that there were no significant differences between the annotations produced by human experts and the result of the proposed deep learning model.</p><p>We conclude that the proposed approach is a robust and time-saving alternative to conventional full-frame confocal imaging analysis of local intracellular Ca<sup>2+</sup> events.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"121 ","pages":"Article 102893"},"PeriodicalIF":4.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000514/pdfft?md5=65d9d2a8d9dd3099c371aa508ba13fc9&pid=1-s2.0-S0143416024000514-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140776277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The tether function of the anoctamins 蛋白拮抗剂的拴系功能
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-20 DOI: 10.1016/j.ceca.2024.102875
Wei-Yin Lin, Woo Young Chung, Shmuel Muallem
{"title":"The tether function of the anoctamins","authors":"Wei-Yin Lin,&nbsp;Woo Young Chung,&nbsp;Shmuel Muallem","doi":"10.1016/j.ceca.2024.102875","DOIUrl":"10.1016/j.ceca.2024.102875","url":null,"abstract":"<div><p>The core functions of the anoctamins are Cl<sup>−</sup> channel activity and phosphatidylserine (and perhaps other lipids) scrambling. These functions have been extensively studied in various tissues and cells. However, another function of the anoctamins that is less recognized and minimally explored is as tethers at membrane contact sites. This short review aims to examine evidence supporting the localization of the anoctamins at membrane contact sites, their tether properties, and their functions as tethers.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"121 ","pages":"Article 102875"},"PeriodicalIF":4.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The physiological roles of anoctamin2/TMEM16B and anoctamin1/TMEM16A in chemical senses 鹅膏蕈素2/TMEM16B和鹅膏蕈素1/TMEM16A在化学感官中的生理作用
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-18 DOI: 10.1016/j.ceca.2024.102889
Michele Dibattista , Simone Pifferi , Andres Hernandez-Clavijo , Anna Menini
{"title":"The physiological roles of anoctamin2/TMEM16B and anoctamin1/TMEM16A in chemical senses","authors":"Michele Dibattista ,&nbsp;Simone Pifferi ,&nbsp;Andres Hernandez-Clavijo ,&nbsp;Anna Menini","doi":"10.1016/j.ceca.2024.102889","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102889","url":null,"abstract":"<div><p>Chemical senses allow animals to detect and discriminate a vast array of molecules. The olfactory system is responsible of the detection of small volatile molecules, while water dissolved molecules are detected by taste buds in the oral cavity. Moreover, many animals respond to signaling molecules such as pheromones and other semiochemicals through the vomeronasal organ. The peripheral organs dedicated to chemical detection convert chemical signals into perceivable information through the employment of diverse receptor types and the activation of multiple ion channels. Two ion channels, TMEM16B, also known as anoctamin2 (ANO2) and TMEM16A, or anoctamin1 (ANO1), encoding for Ca<sup>2+</sup>-activated Cl¯ channels, have been recently described playing critical roles in various cell types. This review aims to discuss the main properties of TMEM16A and TMEM16B-mediated currents and their physiological roles in chemical senses. In olfactory sensory neurons, TMEM16B contributes to amplify the odorant response, to modulate firing, response kinetics and adaptation. TMEM16A and TMEM16B shape the pattern of action potentials in vomeronasal sensory neurons increasing the interspike interval. In type I taste bud cells, TMEM16A is activated during paracrine signaling mediated by ATP. This review aims to shed light on the regulation of diverse signaling mechanisms and neuronal excitability mediated by Ca-activated Cl¯ channels, hinting at potential new roles for TMEM16A and TMEM16B in the chemical senses.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102889"},"PeriodicalIF":4.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140649837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
VSI: The anoctamins: Structure and function VSI:营养素:结构与功能
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-17 DOI: 10.1016/j.ceca.2024.102888
Karl Kunzelmann, Jiraporn Ousingsawat, Rainer Schreiber
{"title":"VSI: The anoctamins: Structure and function","authors":"Karl Kunzelmann,&nbsp;Jiraporn Ousingsawat,&nbsp;Rainer Schreiber","doi":"10.1016/j.ceca.2024.102888","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102888","url":null,"abstract":"<div><p>Plasma membrane localized anoctamin 1, 2 and 6 (TMEM16A, B, F) have been examined in great detail with respect to structure and function, but much less is known about the other seven intracellular members of this exciting family of proteins. This is probably due to their limited accessibility in intracellular membranous compartments, such as the endoplasmic reticulum (ER) or endosomes. However, these so-called intracellular anoctamins are also found in the plasma membrane (PM) which adds to the confusion regarding their cellular role. Probably all intracellular anoctamins except of ANO8 operate as intracellular phospholipid (PL) scramblases, allowing for Ca<sup>2+</sup>-activated, passive transport of phospholipids like phosphatidylserine between both membrane leaflets. Probably all of them also conduct ions, which is probably part of their physiological function. In this brief overview, we summarize key findings on the biological functions of ANO3, 4, 5, 7, 8, 9 and 10 (TMEM16C, D, E, G, H, J, K) that are gradually coming to light. Compartmentalized regulation of intracellular Ca<sup>2+</sup> signals, tethering of the ER to specific PM contact sites, and control of intracellular vesicular trafficking appear to be some of the functions of intracellular anoctamins, while loss of function and abnormal expression are the cause for various diseases.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102888"},"PeriodicalIF":4.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000460/pdfft?md5=f58aed71b2a266518534bd100bcaf091&pid=1-s2.0-S0143416024000460-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140640944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The anoctamins: Structure and function 营养素结构与功能
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-15 DOI: 10.1016/j.ceca.2024.102885
Rainer Schreiber, Jiraporn Ousingsawat, Karl Kunzelmann
{"title":"The anoctamins: Structure and function","authors":"Rainer Schreiber,&nbsp;Jiraporn Ousingsawat,&nbsp;Karl Kunzelmann","doi":"10.1016/j.ceca.2024.102885","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102885","url":null,"abstract":"<div><p>When activated by increase in intracellular Ca<sup>2+</sup>, anoctamins (TMEM16 proteins) operate as phospholipid scramblases and as ion channels. Anoctamin 1 (ANO1) is the Ca<sup>2+</sup>-activated epithelial anion-selective channel that is coexpressed together with the abundant scramblase ANO6 and additional intracellular anoctamins. In salivary and pancreatic glands, ANO1 is tightly packed in the apical membrane and secretes Cl<sup>−</sup>. Epithelia of airways and gut use cystic fibrosis transmembrane conductance regulator (CFTR) as an apical Cl<sup>−</sup> exit pathway while ANO1 supports Cl<sup>−</sup> secretion mainly by facilitating activation of luminal CFTR and basolateral <em>K</em><sup>+</sup> channels. Under healthy conditions ANO1 modulates intracellular Ca<sup>2+</sup> signals by tethering the endoplasmic reticulum, and except of glands its direct secretory contribution as Cl<sup>−</sup> channel might be small, compared to CFTR. In the kidneys ANO1 supports proximal tubular acid secretion and protein reabsorption and probably helps to excrete HCO<sub>3</sub><sup>−</sup>in the collecting duct epithelium. However, under pathological conditions as in polycystic kidney disease, ANO1 is strongly upregulated and may cause enhanced proliferation and cyst growth. Under pathological condition, ANO1 and ANO6 are upregulated and operate as secretory channel/phospholipid scramblases, partly by supporting Ca<sup>2+</sup>-dependent processes. Much less is known about the role of other epithelial anoctamins whose potential functions are discussed in this review.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102885"},"PeriodicalIF":4.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000435/pdfft?md5=6adf751f62121e312218f7d0a3c538d0&pid=1-s2.0-S0143416024000435-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140620607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
pH regulating mechanisms of astrocytes: A critical component in physiology and disease of the brain 星形胶质细胞的 pH 值调节机制:大脑生理和疾病的关键组成部分
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-08 DOI: 10.1016/j.ceca.2024.102882
Shefeeq M. Theparambil , Gulnaz Begum , Christine R. Rose
{"title":"pH regulating mechanisms of astrocytes: A critical component in physiology and disease of the brain","authors":"Shefeeq M. Theparambil ,&nbsp;Gulnaz Begum ,&nbsp;Christine R. Rose","doi":"10.1016/j.ceca.2024.102882","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102882","url":null,"abstract":"<div><p>Strict homeostatic control of pH in both intra- and extracellular compartments of the brain is fundamentally important, primarily due to the profound impact of free protons ([<em>H</em><sup>+</sup>]) on neuronal activity and overall brain function. Astrocytes, crucial players in the homeostasis of various ions in the brain, actively regulate their intracellular [<em>H</em><sup>+</sup>] (pH<sub>i</sub>) through multiple membrane transporters and carbonic anhydrases. The activation of astroglial pH<sub>i</sub> regulating mechanisms also leads to corresponding alterations in the acid-base status of the extracellular fluid. Notably, astrocyte pH regulators are modulated by various neuronal signals, suggesting their pivotal role in regulating brain acid-base balance in both health and disease. This review presents the mechanisms involved in pH regulation in astrocytes and discusses their potential impact on extracellular pH under physiological conditions and in brain disorders. Targeting astrocytic pH regulatory mechanisms represents a promising therapeutic approach for modulating brain acid-base balance in diseases, offering a potential critical contribution to neuroprotection.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102882"},"PeriodicalIF":4.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S014341602400040X/pdfft?md5=03f3d9286d0f6ca600257cdc4408fefb&pid=1-s2.0-S014341602400040X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140555192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous TIRF imaging of subplasmalemmal Ca2+ dynamics and granule fusions in insulin-secreting INS-1 cells reveals coexistent synchronized and asynchronous release 对分泌胰岛素的 INS-1 细胞浆膜下 Ca2+ 动态和颗粒融合进行同步 TIRF 成像,发现同步和非同步释放同时存在
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-08 DOI: 10.1016/j.ceca.2024.102883
Charlotte Suckert , Carolin Zosel , Michael Schaefer
{"title":"Simultaneous TIRF imaging of subplasmalemmal Ca2+ dynamics and granule fusions in insulin-secreting INS-1 cells reveals coexistent synchronized and asynchronous release","authors":"Charlotte Suckert ,&nbsp;Carolin Zosel ,&nbsp;Michael Schaefer","doi":"10.1016/j.ceca.2024.102883","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102883","url":null,"abstract":"<div><p>The basal and glucose-induced insulin secretion from pancreatic beta cells is a tightly regulated process that is triggered in a Ca<sup>2+</sup>-dependent fashion and further positively modulated by substances that raise intracellular levels of adenosine 3′,5′-cyclic monophosphate (cAMP) or by certain antidiabetic drugs. In a previous study, we have temporally resolved the subplasmalemmal [Ca<sup>2+</sup>]<sub>i</sub> dynamics in beta cells that are characterized by trains of sharply delimited spikes, reaching peak values up to 5 µM. Applying total internal reflection fluorescence (TIRF) microscopy and synaptopHluorin to visualize fusion events of individual granules, we found that several fusion events can coincide within 50 to 150 ms. To test whether subplasmalemmal [Ca<sup>2+</sup>]<sub>i</sub> microdomains around single or clustered Ca<sup>2+</sup> channels may cause a synchronized release of insulin-containing vesicles, we applied simultaneous dual-color TIRF microscopy and monitored Ca<sup>2+</sup> fluctuations and exocytotic events in INS-1 cells at high frame rates. The results indicate that fusions can be triggered by subplasmalemmal Ca<sup>2+</sup> spiking. This, however, does account for a minority of fusion events. About 90 %-95 % of fusion events either happen between Ca<sup>2+</sup> spikes or incidentally overlap with subplasmalemmal Ca<sup>2+</sup> spikes. We conclude that only a fraction of exocytotic events in glucose-induced and tolbutamide- or forskolin-enhanced insulin release from INS-1 cells is tightly coupled to Ca<sup>2+</sup> microdomains around voltage-gated Ca<sup>2+</sup> channels.</p></div>","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102883"},"PeriodicalIF":4.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0143416024000411/pdfft?md5=85c9b28c7c9d3d4d9253198eaf4bcd8d&pid=1-s2.0-S0143416024000411-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel probe to monitor lysosome-mitochondria contact sites opens up a new path to study neurodegenerative diseases 监测溶酶体-线粒体接触点的新型探针为研究神经退行性疾病开辟了新途径
IF 4 2区 生物学
Cell calcium Pub Date : 2024-04-08 DOI: 10.1016/j.ceca.2024.102887
Mai Makino , Shuhei Nakamura
{"title":"A novel probe to monitor lysosome-mitochondria contact sites opens up a new path to study neurodegenerative diseases","authors":"Mai Makino ,&nbsp;Shuhei Nakamura","doi":"10.1016/j.ceca.2024.102887","DOIUrl":"https://doi.org/10.1016/j.ceca.2024.102887","url":null,"abstract":"","PeriodicalId":9678,"journal":{"name":"Cell calcium","volume":"120 ","pages":"Article 102887"},"PeriodicalIF":4.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140536794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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