The International journal of developmental biology最新文献_第9页

Visualization of primordial germ cells in the fertilized pelagic eggs of the barfin flounder Verasper moseri. 棘牙鲆远洋受精卵中原始生殖细胞的可视化。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150008rg

R. Goto, Taiju Saito, Y. Kawakami, Tomoe Kitauchi, Misae Takagi, T. Todo, K. Arai, E. Yamaha

{"title":"Visualization of primordial germ cells in the fertilized pelagic eggs of the barfin flounder Verasper moseri.","authors":"R. Goto, Taiju Saito, Y. Kawakami, Tomoe Kitauchi, Misae Takagi, T. Todo, K. Arai, E. Yamaha","doi":"10.1387/ijdb.150008rg","DOIUrl":"https://doi.org/10.1387/ijdb.150008rg","url":null,"abstract":"Primordial germ cells (PGCs) appear during early embryogenesis and differentiate into gametes through oogenesis or spermatogenesis. Teleost PGCs can be visualized by injecting RNA transcribed from the fusion product of a fluorescent protein gene attached to the 3' untranslated region (3'UTR) of zebrafish nanos3 (zf-nos3). Although this method has been widely applied to teleost PGCs, the visualization of PGCs in pelagic species that have eggs with a hard chorion is more problematic due to the technical difficulty of microinjection into their eggs. In this study, we developed a reliable method for microinjection of fertilized eggs in a pelagic species, the barfin flounder. Using a microneedle with a constriction \"brake\", we were able to introduce gfp-nos3 3'UTR mRNA into embryos and to determine the origin and migration route of PGCs. We also isolated the barfin flounder nos3 (bf-nos3) gene to compare its 3'UTR sequence with that of zebrafish. The 3'UTR of the bf-nos3 sequence was longer than that of zf-nos3. However, PGCs were also visualized after injection of gfp-bf-nos3 3'UTR mRNA both in zebrafish and barfin flounder. These results suggest that the function of nos3 is conserved between these species regardless of the sequence differences. The method developed here for labeling PGCs with gfp-nos3 mRNA will provide a means to study PGC development in the embryos of a wide range of marine fish species.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"11 1","pages":"465-70"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81978449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Chicken primordial germ cell motility in response to stem cell factor sensing. 干细胞因子感应对鸡原始生殖细胞运动的影响。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.140287ct

Thanida Srihawong, T. Kuwana, K. Siripattarapravat, C. Tirawattanawanich

{"title":"Chicken primordial germ cell motility in response to stem cell factor sensing.","authors":"Thanida Srihawong, T. Kuwana, K. Siripattarapravat, C. Tirawattanawanich","doi":"10.1387/ijdb.140287ct","DOIUrl":"https://doi.org/10.1387/ijdb.140287ct","url":null,"abstract":"Avian primordial germ cells (PGCs) are destined to migrate a long distance from their extra embryonic region via the vascular system to the gonadal ridges where they form the germ cells. Although PGC migration is crucial for a genetic continuation to the next generation, the factors and mechanisms that control their migration remain largely unknown. In the present study the chemotactic effect of stem cell factor (SCF) was examined on chicken blood circulating PGCs (cPGC), employing 3D chemotaxis slides and time-lapsed imaging analyses as an in vitro study model. Upon in vitro exposure to an SCF gradient, 77.1% (54 out of 70) of cPGCs showed a clear response, of which 48.1% (26 out of 54) polarized with the consecutive formation of a persistent membrane protrusion and significant directional migration towards the gradient and the others showed transient membrane protrusions. In contrast, the controls and apparently SCF unresponsive cPGCs and c-kit-negative red blood cells (RBCs) showed only cytoplasmic cycling with random formations of membrane blebbing and no directional migration. Significant (p < 0.05) differences between the SCF-treated and control cPGCs and RBCs were found in the migration parameters of eccentricity, accumulated and Euclidean distances, and migration velocity. The SCF-treated PGCs also revealed a chemotactic response, as judged by their significant displacement of center of mass and Rayleigh test. Complete inhibition of all the SCF-induced responses in PGCs was found following pretreatment of the cPGCs with 10 µM of the c-kit inhibitor, STI57l, prior to SCF exposure. In addition, cPGCs were found to be positive for c-kit expression using a polyclonal goat anti-mouse c-kit primary antibody, suggesting that the cPGCs were capable of SCF sensing and the potential involvement of SCF/c-kit in the chemotactic migration. Therefore, SCF is suggested to function as a chemoattractant in the migration of chicken cPGC.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"125 1","pages":"453-60"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80006838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

CILP1 is dynamically expressed in the developing musculoskeletal system of the trout. CILP1在鳟鱼发育中的肌肉骨骼系统中动态表达。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150136pr

C. Rallière, M. Frétaud, V. Thermes, P. Rescan

引用次数: 3

iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt. 基于itraq的东方蝾螈肢体再生适应性反应的蛋白质组学分析。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150363cx

X. Geng, Jianlin Guo, Xiayan Zang, Jingyan Sun, Pengfei Li, Fu-chun Zhang, Cunshuan Xu

{"title":"iTRAQ-based proteomic analysis of adaptive response in the regenerating limb of the Cynops orientalis newt.","authors":"X. Geng, Jianlin Guo, Xiayan Zang, Jingyan Sun, Pengfei Li, Fu-chun Zhang, Cunshuan Xu","doi":"10.1387/ijdb.150363cx","DOIUrl":"https://doi.org/10.1387/ijdb.150363cx","url":null,"abstract":"The newt has the powerful capacity to regenerate lost limbs following amputation, and represents an excellent model organism to study regenerative processes. However, the molecular basis of the adaptive response in the regenerating limb of the Chinese fire-bellied newt Cynops orientalis immediately after amputation remains unclear. To better understand the adaptive response immediately after limb amputation at the protein level, we used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS methods to analyze changes in the proteome of the regenerating newt limb that occurred 2 h and 8 h after amputation. We identified 152 proteins with more than 1.5-fold change in expression compared to control. GO annotation analysis classified these proteins into several categories such as signaling, Ca(2+) binding and translocation, transcription and translation, immune response, cell death, cytoskeleton, metabolism, etc. Further ingenuity pathway analysis (IPA) showed that several signaling pathways were significantly changed at 2 h and 8 h after amputation, including EIF2 signaling, acute phase response signaling, tight junction signaling and calcium signaling, suggesting these pathways may be closely related to the adaptive response immediately after limb amputation. This work provides novel insights into understanding the molecular processes related to newt limb regeneration immediately after amputation, and a basis for further study of regenerative medicine.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"79 6 1","pages":"487-96"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76806351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

TRPM channels and magnesium in early embryonic development. TRPM通道和镁在早期胚胎发育中的作用。

The International journal of developmental biology Pub Date : 2015-01-01 DOI: 10.1387/ijdb.150196lr

Y. Komiya, L. Runnels

{"title":"TRPM channels and magnesium in early embryonic development.","authors":"Y. Komiya, L. Runnels","doi":"10.1387/ijdb.150196lr","DOIUrl":"https://doi.org/10.1387/ijdb.150196lr","url":null,"abstract":"Magnesium (Mg(2+)) is the second most abundant cellular cation and is essential for all stages of life, from the early embryo to adult. Mg(2+) deficiency causes or contributes to many human diseases, including migraine headaches, Parkinson's disease, Alzheimer's disease, hypotension, type 2 diabetes mellitus and cardiac arrhythmias. Although the concentration of Mg(2+) in the extracellular environment can vary significantly, the total intracellular Mg(2+) concentration is actively maintained within a relatively narrow range (14 - 20 mM) via tight, yet poorly understood, regulation of intracellular Mg(2+)by Mg(2+) transporters and Mg(2+)-permeant ion channels. Recent studies have continued to add to the growing number of Mg(2+) transporters and ion channels involved in Mg(2+) homeostasis, including TRPM6 and TRPM7, members of the transient receptor potential (TRP) ion channel family. Mutations in TRPM6, including amino acid substitutions that prevent its heterooligomerization with TRPM7, occur in the rare autosomal-recessive disease hypomagnesemia with secondary hypocalcemia (HSH). Genetic ablation of either gene in mice results in early embryonic lethality, raising the question of whether these channels' capacity to mediate Mg(2+) influx plays an important role in embryonic development. Here we review what is known of the function of Mg(2+) in early development and summarize recent findings regarding the function of the TRPM6 and TRPM7 ion channels during embryogenesis.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"82 1","pages":"281-8"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90596340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Corrigendum: Hox collinearity - a new perspective (vol 55, pg 899, 2011) 勘误表:Hox共线性——一个新的视角(第55卷，第899页，2011)

The International journal of developmental biology Pub Date : 2012-04-24 DOI: 10.1387/IJDB.123515CO

A. Durston, H. Jansen, P. I. D. Rieden, M. Hooiveld

引用次数: 1

CORRIGENDUM: Persistent expression of Twist1 in chondrocytes causes growth plate abnormalities and dwarfism in mice 更正:软骨细胞中Twist1的持续表达导致小鼠生长板异常和侏儒症

The International journal of developmental biology Pub Date : 2012-04-24 DOI: 10.1387/IJDB.123532CO

Rosa M. Guzzo, V. Andreeva, D. Spicer, M. Drissi

引用次数: 0

In Memoriam - Miguel Guirao (1924-2010)

The International journal of developmental biology Pub Date : 2010-01-01 DOI: 10.1387/IJDB.113284JA

J. Aréchaga

引用次数: 0

Rotation in Xenopus laevis embryos during the second cell cycle. 非洲爪蟾胚胎在第二次细胞周期中的旋转。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.062266ss

S. M. Starodubov, Vladimir A Golychenkov

引用次数: 2

Hedgehog signalling is required for cloacal development in the zebrafish embryo. 斑马鱼胚胎的泄殖腔发育需要Hedgehog信号。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082669cp

C. Parkin, C. Allen, P. Ingham

{"title":"Hedgehog signalling is required for cloacal development in the zebrafish embryo.","authors":"C. Parkin, C. Allen, P. Ingham","doi":"10.1387/ijdb.082669cp","DOIUrl":"https://doi.org/10.1387/ijdb.082669cp","url":null,"abstract":"The Hedgehog (Hh) family of signalling molecules is essential for a wide range of developmental processes. Mammalian studies have implicated the Hedgehog pathway in the aetiology of anorectal malformations (ARMs), relatively common congenital anomalies caused by failures in the development of the cloaca. In this study we demonstrate that Hh signalling is absolutely required for the formation of the zebrafish cloaca and that the severity of the posterior gut abnormalities induced by a reduction in Hh activity is dependent on the levels of Hh signal transduction. The complete loss of all Hh activity results in the most severe defects and the critical period for Hh activity is between 34 and 74 hours post fertilisation. Using a range of mutant genotypes that cause notochord and floorplate abnormalities, we show that the source of the Hh signals required for posterior gut formation is the endoderm and not the notochord, as previously postulated in mammalian models of ARMs. We show that Adriamycin, a drug known to cause ARMs in rat, but not chick embryos, has no effect on the development of the zebrafish gastrointestinal tract. These studies establish the zebrafish as a model for ARMs, and for the elucidation of other pathways involved in hindgut developmental processes.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"61 1","pages":"45-57"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74069019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34