The International journal of developmental biology最新文献_第10页

Ceratitis capitata transformer-2 gene is required to establish and maintain the autoregulation of Cctra, the master gene for female sex determination. Cctra是决定女性性别的主基因，它的自动调节需要Cctra -2基因的建立和维持。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082681ms

M. Salvemini, M. Robertson, B. Aronson, P. Atkinson, L. C. Polito, G. Saccone

{"title":"Ceratitis capitata transformer-2 gene is required to establish and maintain the autoregulation of Cctra, the master gene for female sex determination.","authors":"M. Salvemini, M. Robertson, B. Aronson, P. Atkinson, L. C. Polito, G. Saccone","doi":"10.1387/ijdb.082681ms","DOIUrl":"https://doi.org/10.1387/ijdb.082681ms","url":null,"abstract":"In Drosophila melanogaster, transformer-2 (TRA-2) which is a non-sex-specific auxiliary splicing factor, is required to promote female sexual differentiation by interaction with the female-specific TRA. The two proteins positively regulate the splicing of both doublesex (dsx) and fruitless (fru) pre-mRNAs, which in turn regulate phenotypic and behavioural sexual dimorphism. In the Mediterranean fruitfly Ceratitis capitata, the female-specific CcTRA is similarly required not only for Ccdsx splicing, but also to exert a novel autoregulatory function that consists of promoting female-specific splicing of Cctra pre-mRNA. This study reports the isolation and functional analysis of the C. capitata homologue of the Drosophila transformer-2 gene (Cctra-2). Transient RNAi against Cctra-2 during embryonic development causes the full sex reversal of XX flies in adult fertile pseudo-males, as well as changes in the splicing pattern of Cctra, Ccdsx and Ccfruitless (Ccfru). We propose that: 1) Cctra-2, as in Drosophila, is necessary for promoting Ccdsx and putative Ccfru pre-mRNA female-specific splicing and that 2) unlike in Drosophila, Cctra-2 appears to be necessary for establishing female sex determination in early XX embryos and for maintaining the positive feedback regulation of Cctra during development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"47 1","pages":"109-20"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84528573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 126

The 2-cell block occurring during development of outbred mouse embryos is rescued by cytoplasmic factors present in inbred metaphase II oocytes. 远交系小鼠胚胎发育过程中发生的2细胞阻滞被近交系中期卵母细胞中的细胞质因子所挽救。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082617mz

M. Zanoni, S. Garagna, C. Redi, M. Zuccotti

{"title":"The 2-cell block occurring during development of outbred mouse embryos is rescued by cytoplasmic factors present in inbred metaphase II oocytes.","authors":"M. Zanoni, S. Garagna, C. Redi, M. Zuccotti","doi":"10.1387/ijdb.082617mz","DOIUrl":"https://doi.org/10.1387/ijdb.082617mz","url":null,"abstract":"In mice, completion of preimplantation development in vitro is restricted to certain crosses between inbred strains. Most of the outbred and inbred strains cease development at the 2-cell stage, a phenomenon known as the \"2-cell block\". Reciprocal mating between blocking and non-blocking strains has shown that the 2-cell block is dependent upon female, but not male, developmental information. One question that still remains unanswered is whether the genome of the metaphase II (MII) oocyte is genetically programmed to express, during the very early stages of development, some factor(s) required to determine developmental competence beyond the 2-cell stage. In the present study, we have addressed this question by performing reciprocal MII-chromosome plate transfer between MII oocytes of a non-blocking inbred strain and MII oocytes of a blocking outbred strain. Here, we report that development beyond the 2-cell stage does not depend on the MII genome, but instead it relies on a cytoplasmic factor(s) already present in ovulated non-blocking oocytes, but absent, inactive or quantitatively insufficient in blocking oocytes. Further evidence of the ooplasmic origin of this component(s) was obtained by transferring a small quantity of ooplasm from non-blocking MII oocytes to blocking MII oocytes or 2-cell embryos. Following the transfer, a high percentage of blocking oocytes/embryos acquired developmental competence beyond the 2-cell stage and reached the blastocyst stage. This study shows that development beyond the 2-cell stage relies also on a factor(s) already present in the ovulated oocyte.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"10 1","pages":"129-34"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88526229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Neural stem cells at the crossroads: MMPs may tell the way. 神经干细胞处于十字路口:MMPs可能会指明方向。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082573gt

G. Tonti, F. Mannello, E. Cacci, S. Biagioni

{"title":"Neural stem cells at the crossroads: MMPs may tell the way.","authors":"G. Tonti, F. Mannello, E. Cacci, S. Biagioni","doi":"10.1387/ijdb.082573gt","DOIUrl":"https://doi.org/10.1387/ijdb.082573gt","url":null,"abstract":"Matrix metalloproteinases (MMP) constitute a family of more than 25 enzymes which process a large number of pericellular substrates. Even though initially reported to have an ability to degrade almost all of the extracellular components, MMP are now known to play roles which are not limited to the breakdown of extracellular barriers. In fact, MMPs regulate many biological processes, being involved not only in physiological events, but also in pathological processes. Strikingly, MMPs have been found to be involved in the physiology of the Central Nervous System (CNS), taking part and playing important roles in several processes such as repair and ontogeny, as well as in pathological conditions of the CNS. Initially considered to be a static structure, lacking regenerative capability, the CNS has been considered for a long time to be a system without renewal capabilities. Recently, the discovery of constant neural replacement has changed our way of considering the adult brain, and the finding of the existence of neural stem cells has opened the way to exciting and fascinating perspectives of the CNS. So, could MMPs, originally found during metamorphosis in tadpoles, and now amazingly identified in the CNS, have something to do in neuronal function? In this review we take into consideration the possible roles of two metalloproteinases, MMP-2 and MMP-9, also called gelatinases, in controlling several aspects of CNS organization, including the modulation of neural stem cell properties and the differentiation of their progeny, both under normal and pathophysiological conditions.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"1 1","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89513435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Generation of a reporter-null allele of Ppap2b/Lpp3and its expression during embryogenesis. Ppap2b/ lpp3报告基因缺失等位基因的产生及其在胚胎发生过程中的表达。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082745de

D. Escalante-Alcalde, Sara Morales, C. Stewart

{"title":"Generation of a reporter-null allele of Ppap2b/Lpp3and its expression during embryogenesis.","authors":"D. Escalante-Alcalde, Sara Morales, C. Stewart","doi":"10.1387/ijdb.082745de","DOIUrl":"https://doi.org/10.1387/ijdb.082745de","url":null,"abstract":"Our knowledge of how bioactive lipids participate during development has been limited principally due to the difficulties of working with lipids. The availability of some of these lipids is regulated by the Lipid phosphate phosphatases (LPPs). The targeted inactivation of Ppap2b, which codes for the isoenzyme Lpp3, has profound developmental defects. Lpp3 deficient embryos die around E9.5 due to extraembryonic vascular defects, making difficult to analyze its participation in later stages of mouse development. To gain some predictive information regarding the possible participation of Lpp3 in later stages of development, we generated a Ppap2b null reporter allele and it was used to establish its expression pattern in E8.5-13.5 embryos. We found that Ppap2b expression during these stages was highly dynamic with significant expression in structures where multiple inductive interactions occur such as the limb buds, mammary gland primordia, heart cushions and valves among others. These observations suggest that Lpp3 expression may play a key role in modulating/integrating multiple signaling pathways during development.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"1 1","pages":"139-47"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78484416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Transcriptional regulation by Pax3 and TGFbeta2 signaling: a potential gene regulatory network in neural crest development. Pax3和TGFbeta2信号的转录调控:神经嵴发育的潜在基因调控网络。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082682hn

H. Nakazaki, Yueh-wei Shen, B. Yun, Anvesh C. Reddy, Anvesh C. Reddy, Varun Khanna, B. Mania‐Farnell, S. Ichi, David G. McLone, T. Tomita, C. Mayanil

引用次数: 14

Genetic evidence against a role for W-linked histidine triad nucleotide binding protein (HINTW) in avian sex determination. 反对w -连锁组氨酸三核苷酸结合蛋白(HINTW)在鸟类性别决定中的作用的遗传证据。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082742cs

C. Smith, K. Roeszler, A. Sinclair

{"title":"Genetic evidence against a role for W-linked histidine triad nucleotide binding protein (HINTW) in avian sex determination.","authors":"C. Smith, K. Roeszler, A. Sinclair","doi":"10.1387/ijdb.082742cs","DOIUrl":"https://doi.org/10.1387/ijdb.082742cs","url":null,"abstract":"Birds have a ZZ/ZW sex chromosome system, but the mechanism of sex determination remains unknown. The heterogametic sex is female (ZW) and one hypothesis holds that the W chromosome carries a dominant-acting ovary-determining gene. The strongest candidate ovary-determinant on the W chromosome is HINTW, which encodes an aberrant nucleotide hydrolase enzyme. HINTW is conserved amongst all carinate (flying) birds and it is strongly expressed in the gonads and other tissues of female chicken embryos. This and other lines of circumstantial evidence support the proposal that HINTW is the female-determining gene in birds. However, in vivo gain-of-function or loss-of-function studies have not hitherto been reported to test this hypothesis. We tested the potential role of HINTW by mis-expressing it in genetically male (ZZ) embryos, using the RCASBP avian retroviral vector. Strong, widespread expression was delivered throughout the embryo, including the urogenital system, as assessed by whole mount in situ hybridisation. This expression pattern mimicked that seen in normal ZW females, in which HINTW is widely expressed. Strong mis-expression was observed throughout the gonads of genetic male (ZZ) embryos. However, despite strong HINTW expression, ZZ gonads developed normally as bilateral testes. In tissue sections of ZZ urogenital systems transgenic for HINTW, normal testicular histology was observed. Female (ZW) gonads over-expressing HINTW also developed normally, with normal ovarian structure and left/right asymmetry. These results provide genetic evidence against a dominant role for HINTW in avian sex determination.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"94 1","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85181262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Organelles in developing neurons: essential regulators of neuronal morphogenesis and function. 神经元发育中的细胞器:神经元形态发生和功能的重要调节因子。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.082618ss

Sayaka Sekine, M. Miura, T. Chihara

引用次数: 16

Arabidopsis monomeric G-proteins, markers of early and late events in cell differentiation. 拟南芥单体g蛋白，细胞分化早期和晚期事件的标记。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.072488mb

Mariette Bedhomme, C. Mathieu, A. Pulido, Y. Henry, C. Bergounioux

{"title":"Arabidopsis monomeric G-proteins, markers of early and late events in cell differentiation.","authors":"Mariette Bedhomme, C. Mathieu, A. Pulido, Y. Henry, C. Bergounioux","doi":"10.1387/ijdb.072488mb","DOIUrl":"https://doi.org/10.1387/ijdb.072488mb","url":null,"abstract":"In Schizosaccharomyces pombe, septum formation is intricately controlled by proteins which constitute the SIN (Septum Initiation Network) signalling cascade. The SIN ensures the coordination between mitotic exit and cytokinesis. Yeast spg1p is a core component of the SIN pathway and we have previously characterized the two orthologs of this G-protein in Arabidopsis thaliana (named AtSGP1 and 2). In this work, the cell and tissue expression of AtSGP genes during plant development has been analysed using AtSGP promoter::GUS fusions in stably transformed A. thaliana lines. AtSGP1 promoter activity was restricted to the quiescent centre, collumella cells, stomata guard cells and the stele while AtSGP2 promoter activity was detected in atrichoblasts, trichomes and pollen. The observed promoter activities are in accordance with publicly available pollen, stomata guard cell and root transcriptome data. Two-hybrid experiments previously evidenced an interaction between AtMAP3Kepsilon1 and AtSGP1. The AtMAP3Kepsilon1 promoter activity was detected in root apices, trichomes and ovule integuments. A genetic approach involving both markers of these specialized cells and mutant backgrounds was used to reinforce our hypothesis. It appears that, although highly conserved between plants and fungi, the spg1p G-protein has evolved in plants to perform a function different from the SIN pathway. Interestingly, cells expressing AtSGPs possessed limited or null mitotic activity. Our data suggests that AtSGP are crucial signalling components involved either in early cell fate specification, or in the final steps of cell differentiation. This is an interesting starting point for a wider study devoted to functional experiments designed to test these hypotheses.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"36 1","pages":"177-85"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76146150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Triiodothyronine (T3) action on aquatic locomotor behavior during metamorphosis of the bullfrog Rana catesbeiana. 三碘甲状腺原氨酸(T3)对牛蛙变形过程中水中运动行为的影响。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.072307mf

Marisabel Fernández-Mongil, Celia J Venza, Amelia Rivera, J. Lasalde-Dominicci, Warren Burggren, L. Rojas

{"title":"Triiodothyronine (T3) action on aquatic locomotor behavior during metamorphosis of the bullfrog Rana catesbeiana.","authors":"Marisabel Fernández-Mongil, Celia J Venza, Amelia Rivera, J. Lasalde-Dominicci, Warren Burggren, L. Rojas","doi":"10.1387/ijdb.072307mf","DOIUrl":"https://doi.org/10.1387/ijdb.072307mf","url":null,"abstract":"Thyroid hormones--particularly triiodothyronine, T3--play a critical role in the morphological transformations comprising metamorphosis in larval bullfrogs (Rana catesbeiana). Traditional staging criteria for anuran larvae incompletely distinguish physiological and behavioral changes during growth. We therefore first developed a new parameter to describe larval growth, the developmental index (DI), which is simply the ratio between the tail length of the larva and its head diameter. Using the DI we were able to identify two distinct populations classifying the larvae during growth along a continuous linear scale with a cutoff value of DI at 2.8. Classification based on the DI, used in this study, proved an effective complement to existing classifications based on developmental staging into pre- or pro-metamorphic stages. Exposure to T3 in the water induced a rapid (beginning within 5 min) and significant decrease (approximately 20-40%) in locomotor activity, measured as total distance traversed and velocity. The largest decrease occurred in more developed larvae (DI<2.8). To determine correlated changes in the neuromuscular junctions during metamorphosis and apoptotic tail loss, miniature endplate currents from tail muscle were recorded during acute exposure to a hypertonic solution, which simulates an apoptotic volume decrease. Our results support a role for T3 in regulating larval locomotor activity during development, and suggest an enhanced response to volume depletion at the neuromuscular junction of older larvae (DI<2.8) compared to younger animals (DI> or =2.8). We discuss the significance of the possible role of an apoptotic volume decrease at the level of the neuromuscular junction.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":"78 1","pages":"101-8"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83639659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Xenopus tropicalis HNF6/Onecut-1. 热带爪蟾HNF6/Onecut-1的表达。

The International journal of developmental biology Pub Date : 2009-01-01 DOI: 10.1387/ijdb.072472ke

K. Haworth, B. Latinkić

引用次数: 8