SDF-1控制体肌和血管的形成。

Aisha Abduelmula, Ruijin Huang, Q. Pu, H. Tamamura, Gabriela Morosan-Puopolo, B. Brand-Saberi
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引用次数: 10

摘要

基质细胞衍生因子-1 (SDF-1)是趋化因子受体CXCR4的唯一配体,参与骨骼肌的发育。然而,其在somite细胞增殖、分化和迁移中的作用尚不清楚。本文通过功能获得和功能丧失实验,研究了其在鸡胚体发育过程中的功能。SDF-1的过表达是通过将SDF-1构建物电穿孔到体细胞的腹外侧部分,或将表达SDF-1的细胞注射到HH14-16期鸡胚胎的体细胞中来实现的。我们发现,增强的SDF-1信号可以诱导体细胞增殖。这导致肌瘤细胞和内皮细胞的数量增加。相反,抑制SDF-1/CXCR4信号导致肌瘤细胞减少。将产生SDF-1的细胞注射到小体中诱导硬核组肌瘤细胞异位定位。尽管许多表达sdf -1的体细胞定植在肢体上,但只有少数发育成肌肉细胞。这导致了肢体肌肉量的减少。这意味着,由于体细胞中SDF-1信号的高浓度,大多数肌源性祖细胞停止了向肢体的迁移。在肢体中发现的表达sdf -1的somite细胞大部分是内皮细胞,它们有助于肢体血管的增加。这些结果表明,SDF-1促进体原肌祖细胞和血管生成祖细胞的增殖,并控制肌瘤的形成。此外,SDF-1以不同的方式控制肢体肌肉和血管的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SDF-1 controls the muscle and blood vessel formation of the somite.
Stromal-cell-derived factor-1 (SDF-1), the only ligand of the chemokine receptor CXCR4, is involved in skeletal muscle development. However, its role in the proliferation, differentiation and migration of somite cells is not well understood. Here, we investigated its function during somite development in chicken embryos by using gain-of-function and loss-of-function experiments. Overexpression of SDF-1 was performed by electroporating SDF-1 constructs into the ventrolateral part of the somite, or by injecting SDF-1-expressing cells into the somites of stages HH14-16 chicken embryos. We found that enhanced SDF-1 signaling induced cell proliferation in the somite. This resulted in an increase in number of both myotomal and endothelial cells. In contrast, inhibition of SDF-1/CXCR4 signaling led to a reduction of myotomal cells. Injection of SDF-1 producing cells into the somite induced ectopic localization of myotomal cells in the sclerotome. Although many SDF-1-expressing somite cells colonized the limb, only a few of them developed into muscle cells. This resulted in a reduction of the limb muscle mass. This means that most myogenic progenitors were stopped on their migration towards the limb due to the high concentration of the SDF-1 signal in the somite. Most of the SDF-1-expressing somite cells found in the limb were of endothelial cell fate and they contributed to the increase in limb blood vessels. These results reveal that SDF-1 promotes the proliferation of both myogenic and angiogenic progenitor cells of the somite and controls myotome formation. Furthermore, SDF-1 controls muscle and blood vessel formation in the limb in different ways.
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